SOX2 Antibody

Images

 
Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.
Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.
Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more
Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by ...read more
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Sh, ChSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SOX2 Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:125 - 1:250
  • Immunohistochemistry-Paraffin 1:125 - 1:250
  • Western Blot 0.5 ug/mL
Application Notes
This SOX2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot analysis, where a band is observed at ~40 kDa representing the SOX2 protein.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
SOX2 Overexpression Lysate
Control Peptide
SOX2 Peptide (NB110-37235PEP)
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB110-37235 in the following applications:

Publications
Read Publications using
NB110-37235 in the following applications:

  • 1 publication
  • 8 publications
  • IHC
    4 publications
  • 2 publications
  • KD
    1 publication
  • WB
    9 publications

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31690408).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOX2 Antibody

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-37235
Species: Hu, Mu, Rt, Ca, Sh, Ch
Applications: WB, Flow, ICC/IF, IHC, IHC-P

Publications for SOX2 Antibody (NB110-37235)(25)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, IHC-P, KD, WB.


Filter By Application
Flow
(1)
ICC/IF
(8)
IHC
(4)
IHC-P
(2)
KD
(1)
WB
(9)
All Applications
Filter By Species
Human
(15)
Mouse
(7)
Rat
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB110-37235 Applications Species
Lovnicki JM, Gan Y, Feng T et al. LIN28B promotes the development of neuroendocrine prostate cancer J. Clin. Invest. Jul 7 2020 [PMID: 32634132] (IHC-P, Human) IHC-P Human
Peskova L, Jurcikova D, Vanova T, Krivanek J miR-183/96/182 cluster is an important morphogenetic factor targeting PAX6 expression in differentiating human retinal organoids bioRxiv Jan 1 2020 (Flow, Human) Flow Human
Lovnicki JM LIN28B confers cancer stem-like phenotypes for neuroendocrine prostate cancer progression Thesis (IHC) IHC
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J. Clin. Invest. May 19 2020 [PMID: 32427586] (WB, KD, Human) WB, KD Human
Chien HJ, Ying TH, Hsieh SC et al. alpha-Mangostin attenuates stemness and enhances cisplatin-induced cell death in cervical cancer stem-like cells through induction of mitochondrial-mediated apoptosis J. Cell. Physiol. Jan 20 2020 [PMID: 31960449] (WB, Human) WB Human
Cornax I, Pluhar G, Clark H, O'Sullivan M Oligodendroglioma with Neuronal Differentiation in Two Boxer Dogs Journal of Comparative Pathology Oct 1 2019 [PMID: 31690408] (IHC-P, Canine) IHC-P Canine
Gagliano O, Luni C, Qin W et al. Microfluidic reprogramming to pluripotency of human somatic cells Nat Protoc Mar 1 2019 [PMID: 30809022] (ICC/IF, WB, Human) ICC/IF, WB Human
Dun XP, Carr L, Woodley PK et al. Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge Cell Rep Feb 5 2019 [PMID: 30726731] (WB, Rat) WB Rat
Saijo A, Goto H, Nakano M et al. Bone marrow-derived fibrocytes promote stem cell-like properties of lung cancer cells. Cancer Lett 2018 May 1 [PMID: 29448000] (Human) Human
Roberts SL, Dun XP, Doddrell RDS et al. Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve Development 2017 Jul 25 [PMID: 28743796] (WB, Mouse) WB Mouse
Show All 25 Publications.

Reviews for SOX2 Antibody (NB110-37235) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB110-37235:
Filter by Applications
WB
(3)
ICC
(1)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
Western Blot SOX2 NB110-37235
Enlarge
4
reviewed by:
Verified Customer
WB Human 05/18/2020
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotL-1
Western Blot SOX2 NB110-37235
Enlarge
3
reviewed by:
Verified Customer
WB Human 10/11/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman head and neck cancer cell lines
SpeciesHuman
LotL-1
Immunocytochemistry SOX2 NB110-37235
Enlarge
5
reviewed by:
Verified Customer
ICC Human 04/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedaptient derived adgerent glioblastoma stem cells,patient-derived glioblastoma stem cells (adherent on laminin coating)
SpeciesHuman
LotL-3

Comments

Commentsdilution 1:250 in antibody buffer (1% BSA, 0.1% triton X in PBS)
  5
reviewed by:
Verified Customer
WB Human 12/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedOral cancer cell line
SpeciesHuman
CommentsGood antibody for testing in Western Blot. Clear signal at 40 kDa.

Blocking

Blocking DetailsBlocking Buffer: 5% Skim Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP-conjugated anti-rabbit IgG (Perkin Elmer)

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 40 kDa

Comments

CommentsGood antibody for testing in Western Blot. Clear signal at 40 kDa.

Product General Protocols

View specific protocols for SOX2 Antibody (NB110-37235): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOX2 Antibody (NB110-37235). (Showing 1 - 3 of 3 FAQs).

  1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
    • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
  2. As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.
    • This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For SOX2 Antibody NB110-37235, the exact immunogen is available to me in our internal notes. The UniProt Blast of this gave a 100% match with the (again, unfortunately unreviewed) horse entries F6PUQ2 and B6VA41. A 100% match indicates that the antibody should be suitable for you. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.
  3. Can you advise me the pH value for the citrate buffer?
    • For the validation of this antibody in IHC-P, we performed antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0. Please note that Domyan et al 2011 from University of Wisconsin-Madison (USA) have cited the use of this antibody in IHC-P application in their experiments published in - Development (2011) 138, 971-981 Biologists site.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-37235AF350
Alexa Fluor 405 NB110-37235AF405
Alexa Fluor 488 NB110-37235AF488
Alexa Fluor 532 NB110-37235AF532
Alexa Fluor 594 NB110-37235AF594
Alexa Fluor 647 NB110-37235AF647
Alexa Fluor 700 NB110-37235AF700
Alexa Fluor 750 NB110-37235AF750
Biotin NB110-37235B
DyLight 350 NB110-37235UV
DyLight 405 NB110-37235V
DyLight 488 NB110-37235G
DyLight 550 NB110-37235R
DyLight 594 NB110-37235DL594
DyLight 650 NB110-37235C
DyLight 680 NB110-37235FR
DyLight 755 NB110-37235IR
FITC NB110-37235F
HRP NB110-37235H
Janelia Fluor 549 NB110-37235JF549
Janelia Fluor 646 NB110-37235JF646
PE NB110-37235PE

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Bioinformatics Tool for SOX2 Antibody (NB110-37235)

Discover related pathways, diseases and genes to SOX2 Antibody (NB110-37235). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for SOX2 Antibody (NB110-37235)

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Blogs on SOX2. Showing 1-10 of 12 blog posts - Show all blog posts.


  Read full blog post.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to ...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

4.3
5
2
4
1
3
1
2
0
1
0

Verified Customer
05/18/2020
Application: WB
Species: Human

Verified Customer
10/11/2018
Application: WB
Species: Human

Verified Customer
04/24/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SOX2
Entrez
Uniprot