SOX2 Antibody - BSA Free

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.

Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

• Reactivity: Hu, Mu, Rt, Ca, Sh, Ch
• Applications: WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SOX2 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]
• Localization: Nuclear
• Marker: Embryonic Stem Cell Marker
• Predicted Species: Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow (Intracellular) 1:100
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:250
  • Immunohistochemistry 1:125 - 1:250
  • Immunohistochemistry-Paraffin 1:125 - 1:250
  • Western Blot 0.5 ug/mL
• Application Notes: This SOX2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot analysis, where a band is observed at ~40 kDa representing the SOX2 protein.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  SOX2 Overexpression Lysate

• Control Peptide:

  SOX2 Peptide (NB110-37235PEP)

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 31690408).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOX2 Antibody - BSA Free

• ANOP3
• MCOPS3
• MGC2413
• SOX2
• SRY (sex determining region Y)-box 2
• SRY-related HMG-box gene 2
• transcription factor SOX2
• transcription factor SOX-2

Background

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX2 Antibody (NB110-37235)(27)

Publications using NB110-37235

• Yang Y, Chen C, Zuo Q Et al. NARF is a hypoxia-induced coactivator for OCT4-mediated breast cancer stem cell specification Sci Adv 2022-12-09 [PMID: 36490339] (WB, Human)
  
  Details:
  Citation using the DyLight 405 version of this antibody.
• Duan S, Sawyer TW, Sontz RA et al. GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming Cellular and molecular gastroenterology and hepatology 2022-07-11 [PMID: 35835391] (ICC, Mouse)
• Peskova L, Jurcikova D, Vanova T, Krivanek J miR-183/96/182 cluster is an important morphogenetic factor targeting PAX6 expression in differentiating human retinal organoids Stem Cells 2020-09-03 [PMID: 32875669]
• Lovnicki JM, Gan Y, Feng T et al. LIN28B promotes the development of neuroendocrine prostate cancer J. Clin. Invest. 2020-07-07 [PMID: 32634132] (IHC-P, Human)
• Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J Clin Invest. 2020-05-19 [PMID: 32427586] (KD, WB, Human)
• Chien HJ, Ying TH, Hsieh SC et al. alpha-Mangostin attenuates stemness and enhances cisplatin-induced cell death in cervical cancer stem-like cells through induction of mitochondrial-mediated apoptosis J. Cell. Physiol. 2020-01-20 [PMID: 31960449] (WB, Human)
• Cornax I, Pluhar G, Clark H, O'Sullivan M Oligodendroglioma with Neuronal Differentiation in Two Boxer Dogs Journal of Comparative Pathology 2019-10-01 [PMID: 31690408] (IHC-P, Canine)
• Gagliano O, Luni C, Qin W et al. Microfluidic reprogramming to pluripotency of human somatic cells Nat Protoc 2019-03-01 [PMID: 30809022] (WB, ICC/IF, Human)
• Dun XP, Carr L, Woodley PK et al. Macrophage-Derived Slit3 Controls Cell Migration and Axon Pathfinding in the Peripheral Nerve Bridge Cell Rep 2019-02-05 [PMID: 30726731] (WB, Rat)
• Saijo A, Goto H, Nakano M et al. Bone marrow-derived fibrocytes promote stem cell-like properties of lung cancer cells. Cancer Lett 2018-05-01 [PMID: 29448000] (Human)
• Roberts SL, Dun XP, Doddrell RDS et al. Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve Development 2017-07-25 [PMID: 28743796] (WB, Mouse)
• Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca 2+ Release Stimulates Breast Cancer Stem Cell Enrichment Cell Rep. 2017-02-21 [PMID: 28228260] (WB, Human)
  
  Details:
  The positive impact of HIF inhibitors on breast cancer chemotherapy is explored through GSTO1 knockdown.
• Alic I, Kosi N, Kapuralin K et al. Neural stem cells from mouse strain Thy1 YFP-16 are a valuable tool to monitor and evaluate neuronal differentiation and morphology. Neurosci. Lett. 2016-10-03 [PMID: 27712955] (ICC/IF, Mouse)
• Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016-08-31 [PMID: 27590511] (WB, Human)
• Liu L, Liu C, Zhang Q et al. SIRT1-Mediated Transcriptional Regulation of SOX2 Is Important for Self-Renewal of Liver Cancer Stem Cells. Hepatology 2016-06-16 [PMID: 27312708] (IF/IHC, Human)
• Luni C, Giulitti S, Serena E et al. High-efficiency cellular reprogramming with microfluidics. Nat. Methods. 2016-05-01 [PMID: 27088312] (ICC/IF, Human)
• Rad A, Farshchian M, Forghanifard MM et al. Predicting the molecular role of MEIS1 in esophageal squamous cell carcinoma. Tumour Biol. 2015-08-28 [PMID: 26314854] (Human)
• Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015-07-30 [PMID: 26229077] (WB, Human)
• Lee YC, Yu CC, Lan C et al. Plasminogen activator inhibitor-1 regulates tumor initiating cell properties in head and neck cancers Head Neck. 2015-01-01 [PMID: 25974262] (WB, Human)
• Lovnicki JM LIN28B confers cancer stem-like phenotypes for neuroendocrine prostate cancer progression Thesis (IF/IHC)
• El-Sayed AK, Zhang Z, Zhang L et al. Pluripotent state induction in mouse embryonic fibroblast using mRNAs of reprogramming factors. Int J Mol Sci 2014-01-01 [PMID: 25437916] (ICC/IF, Mouse)
• Wu S, Wu Y, Zhang X, Capecchi MR. Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Proc. Natl. Acad. Sci. U.S.A. 2014-07-22 [PMID: 25002522] (ICC/IF, Mouse)
  
  Details:
  SOX2 antibody used for ICC-IF on iPS cells (derived from MEFs of strain 129Sv) that were fixed in 4% paraformaldehyde 30 minutes RT. Fig. 2B - Staining of endogenous pluripotency genes (Oct4, Sox2, Nanog, and Ssea1) on transgene-free iPSZX11-18-2 cells demonstrating that the genes are active after the loss of pMaster12 vector.
• Ghods AJ, Glick R, Braun D, Feinstein D. Beneficial actions of the anti-inflammatory dimethyl fumarate in glioblastomas. Surg Neurol Int 2013-12-24 [PMID: 24404403] (ICC/IF, Mouse, Human)
• Grigoryan T, Stein S, Qi J et al. Wnt/Rspondin/beta-catenin signals control axonal sorting and lineage progression in Schwann cell development. Proc Natl Acad Sci U S A. 2013-11-05 [PMID: 24151333] (ICC/IF, Mouse)
• Yu S-C, Xiao H-L, Jiang X-F et al. Connexin 43 Reverses Malignant Phenotypes of Glioma Stem Cells by Modulating E-Cadherin. Stem Cells (Dayton, Ohio). 2011-11-30 [PMID: 22131169]
• Domyan ET, Ferretti E, Throckmorton K et al. Signaling through BMP receptors promotes respiratory identity in the foregut via repression of Sox2. Development;138(5):971-981. 2011-03-01 [PMID: 21303850] (ICC/IF, IF/IHC, Mouse)
• Sholl Lynette M, Barletta Justine A, Yeap Beow Y et al. Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma. The American Journal of Surgical Pathology 2010-01-01 [PMID: 20631605] (IF/IHC, Human)

Reviews for SOX2 Antibody (NB110-37235) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Human.

reviewed by: Jie Lan WB Human 05/18/2020

Summary

• Application: Western Blot
• Sample Tested: Breast cancer cells
• Species: Human
• Lot: L-1

reviewed by: Haiquan Lu WB Human 10/11/2018

Summary

• Application: Western Blot
• Sample Tested: Human head and neck cancer cell lines
• Species: Human
• Lot: L-1

reviewed by: chiara pastori ICC Human 04/24/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: aptient derived adgerent glioblastoma stem cells,patient-derived glioblastoma stem cells (adherent on laminin coating)
• Species: Human
• Lot: L-3

Comments

• Comments: dilution 1:250 in antibody buffer (1% BSA, 0.1% triton X in PBS)

reviewed by: Wen- Wei Chang WB Human 12/27/2011

Summary

• Application: Western Blot
• Sample Tested: Oral cancer cell line
• Species: Human

FAQs for SOX2 Antibody (NB110-37235). (Showing 1 - 3 of 3 FAQs).

1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
   • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
2. As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.
   • This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For SOX2 Antibody NB110-37235, the exact immunogen is available to me in our internal notes. The UniProt Blast of this gave a 100% match with the (again, unfortunately unreviewed) horse entries F6PUQ2 and B6VA41. A 100% match indicates that the antibody should be suitable for you. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.
3. Can you advise me the pH value for the citrate buffer?
   • For the validation of this antibody in IHC-P, we performed antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0. Please note that Domyan et al 2011 from University of Wisconsin-Madison (USA) have cited the use of this antibody in IHC-P application in their experiments published in - Development (2011) 138, 971-981 Biologists site.

Bioinformatics

• Gene Symbol: SOX2
• Entrez:
  • 6657(Human)
  • 20674(Mouse)
• Uniprot:
  • P48431()