SOX2 Antibody

Images

 
Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 (0.5ug/ml).
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.
Flow Cytometry: SOX2 Antibody [NB110-37235] - Staining of NTERA-2 cells using NB110-37235 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit IgG secondary antibody.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NB110-37235] - ICC/IF staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI ...read more

Product Details

Summary
Reactivity Hu, Mu, Sh, ChSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SOX2 Antibody Summary

Immunogen
A synthetic peptide made to the N-terminal region of human SOX2 protein (within residues 1-100). [Swiss-Prot# P48431]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOX2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:125-1:250
  • Immunohistochemistry-Paraffin 1:125-1:250
Application Notes
This SOX2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Western Blot analysis, where a band is observed at ~40 kDa representing the SOX2 protein.
Positive Control
Brain Lysate (NB820-59657)
SOX2 Lysate (NBL1-16348)
Brain Diencephalon Lysate (NB820-59471)
Control Peptide
SOX2 Peptide (NB110-37235PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB110-37235 in the following applications:

Publications
Read Publications using
NB110-37235 in the following applications:

  • 7 publications
  • IHC
    2 publications
  • WB
    5 publications

Reactivity Notes

Human, sheep and mouse. 92% sequence identity with chicken, 86% sequence identity with Xenopus, and 71% sequence identity with Zebrafish proteins.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOX2 Antibody

  • ANOP3
  • MCOPS3
  • MGC2413
  • SOX2
  • SRY (sex determining region Y)-box 2
  • SRY-related HMG-box gene 2
  • transcription factor SOX2
  • transcription factor SOX-2

Background

SOX2 (SRY-RELATED HMG-BOX GENE 2), the basic helix-loop-helix transcription factor, is indispensable for maintaining embryonic stem (ES) cell pluripotency. The gene expresses in self-renewing progenitor cells and acts to inhibit neuronal differentiation. SOX2, along with other transcription factors Oct3/4, klf4, and c-Myc are used to generate induced pluripotent stem cells from mouse fibroblasts by retrovirus mediated introduction. It is expressed throughout the human brain, including the developing hypothalamus, as well as the Rathke pouch, the developing anterior pituitary and the eye. It is necessary for the normal development of the hypothalamo-pituitary and reproductive axes in mice. Mutation of the SOX2 gene in progenitor cells leads to cerebral malformation, severe eye defects, and hypothalamo-pituitary abnormalities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX2 Antibody (NB110-37235)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(7)
IHC
(2)
WB
(5)
All Applications
Filter By Species
Human
(8)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB110-37235 Applications Species
Roberts SL, Dun XP, Doddrell RDS et al. Sox2 expression in Schwann cells inhibits myelination in vivo and induces influx of macrophages to the nerve Development 2017 Jul 25 [PMID: 28743796] (WB, Mouse) WB Mouse
Lu H, Chen I, Shimoda LA et al. Chemotherapy-Induced Ca2+ Release Stimulates Breast Cancer Stem Cell Enrichment. Cell Rep. [PMID: 28228260] (WB, Human) WB Human
Alic I, Kosi N, Kapuralin K et al. Neural stem cells from mouse strain Thy1 YFP-16 are a valuable tool to monitor and evaluate neuronal differentiation and morphology. Neurosci. Lett. Oct 3 2016 [PMID: 27712955] (ICC/IF, Mouse) ICC/IF Mouse
Zhang C, Zhi WI, Lu H et al. Hypoxia-inducible factors regulate pluripotency factor expression by ZNF217- and ALKBH5-mediated modulation of RNA methylation in breast cancer cells. Oncotarget. 2016 Aug 31 [PMID: 27590511] (WB, Human) WB Human
Liu L, Liu C, Zhang Q et al. SIRT1-Mediated Transcriptional Regulation of SOX2 Is Important for Self-Renewal of Liver Cancer Stem Cells. Hepatology Jun 16 2016 [PMID: 27312708] (IHC, Human) IHC Human
Luni C, Giulitti S, Serena E et al. High-efficiency cellular reprogramming with microfluidics. Nat. Methods. 2016 May 01 [PMID: 27088312] (ICC/IF, Human) ICC/IF Human
Rad A, Farshchian M, Forghanifard MM et al. Predicting the molecular role of MEIS1 in esophageal squamous cell carcinoma. Tumour Biol. 2015 Aug 28 [PMID: 26314854] (Human) Human
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015 Jul 30 [PMID: 26229077] (WB, Human) WB Human
Lee YC, Yu CC, Lan C et al. Plasminogen activator inhibitor-1 regulates tumor initiating cell properties in head and neck cancers Head Neck. 2015 [PMID: 25974262] (WB, Human) WB Human
El-Sayed AK, Zhang Z, Zhang L et al. Pluripotent state induction in mouse embryonic fibroblast using mRNAs of reprogramming factors. Int J Mol Sci 2014 [PMID: 25437916] (ICC/IF, Mouse) ICC/IF Mouse
Show All 15 Publications.

Reviews for SOX2 Antibody (NB110-37235) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: ICC, WB.

Reviews using NB110-37235:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry SOX2 NB110-37235
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reviewed by:
Anonymous
ICC Human 04/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedaptient derived adgerent glioblastoma stem cells,patient-derived glioblastoma stem cells (adherent on laminin coating)
SpeciesHuman
LotL-3

Comments

Commentsdilution 1:250 in antibody buffer (1% BSA, 0.1% triton X in PBS)
 
reviewed by:
Anonymous
WB Human 12/27/2011
View

Summary

ApplicationWestern Blot
Sample TestedOral cancer cell line
SpeciesHuman
CommentsGood antibody for testing in Western Blot. Clear signal at 40 kDa.

Blocking

Blocking DetailsBlocking Buffer: 5% Skim Milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: HRP-conjugated anti-rabbit IgG (Perkin Elmer)

Details

Detection NotesDetection Method: ECL Plus, Specific Bands: 40 kDa

Comments

CommentsGood antibody for testing in Western Blot. Clear signal at 40 kDa.

Product General Protocols

View specific protocols for SOX2 Antibody (NB110-37235): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOX2 Antibody (NB110-37235). (Showing 1 - 3 of 3 FAQs).

  1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
    • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.
  2. As part of an Endometriosis-related project in horses, I would like to ask some information with regard to this. I would like to know about its tested or predicted reactivity with horse.
    • This antibody has not yet been tested with horse. This means that use of this antibody with horse would not be covered by our guarantee; however, you would be eligible for our Innovator's Reward Program. For an indication of the likely cross-reactivity (how well the antibody is likely to perform with horse), I have run UniProt Blast sequence comparison of the immunogen sequence versus horse proteins. For SOX2 Antibody NB110-37235, the exact immunogen is available to me in our internal notes. The UniProt Blast of this gave a 100% match with the (again, unfortunately unreviewed) horse entries F6PUQ2 and B6VA41. A 100% match indicates that the antibody should be suitable for you. If you would be interested in testing this novel species, please take a look at our Innovators Reward Program.
  3. Can you advise me the pH value for the citrate buffer?
    • For the validation of this antibody in IHC-P, we performed antigen retrieval using 0.01 M sodium citrate buffer, pH 6.0. Please note that Domyan et al 2011 from University of Wisconsin-Madison (USA) have cited the use of this antibody in IHC-P application in their experiments published in - Development (2011) 138, 971-981 Biologists site.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-37235AF405
Alexa Fluor 488 Labeled NB110-37235AF488
Alexa Fluor 647 Labeled NB110-37235AF647
Alexa Fluor 700 Labeled NB110-37235AF700
Biotin Labeled NB110-37235B
DyLight 405 Labeled NB110-37235V
DyLight 488 Labeled NB110-37235G
DyLight 550 Labeled NB110-37235R
DyLight 650 Labeled NB110-37235C
FITC Labeled NB110-37235F
HRP Labeled NB110-37235H
PE Labeled NB110-37235PE

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Bioinformatics Tool for SOX2 Antibody (NB110-37235)

Discover related pathways, diseases and genes to SOX2 Antibody (NB110-37235). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for SOX2 Antibody (NB110-37235)

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Blogs on SOX2.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

SOX2 - a stem cell transcription factor
The SOX gene family encodes a group of highly conserved transcription factors defined by the presence of a conserved high motility group (HMG) DNA-binding domain. They are involved in embryonic development regulation and cell fate determination. Al...  Read full blog post.

SOX2: an Important Stem Cell Transcription Factor
SOX2 is a transcription factor that is expressed by self-renewing and multipotent stem cells of the embryonic neuroepithelium. Sox-2 was found to be expressed by dividing neural progenitor cells. Constitutive expression of SOX2 has also been shown to...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Recent Reviews

5
5
2
4
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0
2
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1
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Anonymous
04/24/2017
Application: ICC
Species: Human

 
Anonymous
12/27/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol SOX2
Entrez
Uniprot