Nestin Antibody (196908) [Unconjugated]

Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

A172 cells were stained with Mouse Anti-Human Nestin Monoclonal Antibody (Catalog # MAB1259, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and DIV 60 labeled for total alpha -syn, PS129, TH (DA neuronal marker), nestin (NPC marker) or GAPDH show that levels of PS129-modified alpha -syn are elevated in A53T hNs at DIV 14 and remain elevated at DIV 60 relative to corrected cells. b, c Mitochondria were purified from A53T cells and genetically corrected cells at both DIV 14 and DIV 60. Lysates were then separated based on TX-100 solubility (soluble) or urea solubility (insoluble). Western analysis of A53T cells shows elevated soluble alpha -syn at the mitochondria relative to corrected cells at DIV 14 and DIV 60 (denoted by ** and * respectively). Quantification of soluble and insoluble alpha -syn levels in mitochondrial fractions normalized to Coomassie (c). Data represent mean ± s.e.m. *P < 0.05, **P < 0.01 by ANOVA followed by Tukey’s post hoc test, n = 4. d, e Micrographs of corrected and A53T-mutant NPCs expressing the negative charge probe RPRE-RFP and CL-GFP show RPRE-RFP translocation from a primarily plasma membrane localization in isogenic-corrected hNs to a punctate intracellular localization in A53T hNs in tight cellular localization with CL-GFP (d). Quantification of percent total cell number with colocalized RPRE-RFP and CL-GFP (e). Data represent mean ± s.e.m. **P < 0.0001 by t-test, n = 3 coverslips over 2 independent differentiations, DIV: 14; scale bar: 10 μm. f, g Fluorescence resonance energy transfer (FRET) from CL-GFP to RPRE-RFP in hiPSC-derived A53T and corrected hNs or hESC-derived WT, A53T and E46K hNs was assessed (f) and mean FRET intensity was quantified (g). Data represent mean ± s.e.m. **P < 0.01 by ANOVA followed by Tukey’s post hoc test, for hiPSCs, n = 6, for hESCs, n = 8 coverslips over two independent differentiations, DIV: 14. Scale bar: 10 µm. h Mitochondria were isolated from the SNpc of 6-month-old WT and A53T transgenic animals and labeled with Annexin V to measure the abundance of cardiolipin on the mitochondrial surface. Controls were rotenone-exposed WT samples. Animals were not randomized, nor was the analysis blinded. Data represent mean ± s.e.m. **P < 0.01 by ANOVA followed by Dunnett post hoc test, n = 4. Clipart was obtained at clker.com Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03241-9), licensed under a CC-BY license. Not internally tested by R&D Systems.

MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived orthotopic brain tumors from vehicle (CTRL), TMZ or TMZ/metformin (Combo) treated mice were analyzed for Ki67, Nestin and CD133 markers by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. H&E was performed for morphological evaluation of the tumors. Representative images are shown with original magnification x200. (C) Variation of T98G tumor volume measured using caliper after treatment expressed as percentage of volume variation. In T98G xenograft model already at 7 days, the combined treatment significantly slowed down tumor growth compared to control (p value = 0.005), moreover a transient reduction was observed with MET (p value = 0.03). After 3 weeks, only Combo-treated mice displayed tumor smaller than vehicle (p value = 0.04). Error bars indicate SEM. (D) T98G tumors from vehicle (CTRL), TMZ, MET or TMZ/metformin (Combo) treated mice, were analyzed for Ki67, Nestin and CD133 markers expression by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. Hematoxylin and eosin staining (H&E) was performed for morphological evaluation of the tumors. Representative images are shown with Original magnification x200. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23028), licensed under a CC-BY license. Not internally tested by R&D Systems.

MET and TMZ effects in in vivo glioma models(A) Variation of U251 tumor volume measured at contrast-enhanced T1 MRI after treatment expressed as percentage of volume change. Error bars indicate SEM. (B) U251 derived orthotopic brain tumors from vehicle (CTRL), TMZ or TMZ/metformin (Combo) treated mice were analyzed for Ki67, Nestin and CD133 markers by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. H&E was performed for morphological evaluation of the tumors. Representative images are shown with original magnification x200. (C) Variation of T98G tumor volume measured using caliper after treatment expressed as percentage of volume variation. In T98G xenograft model already at 7 days, the combined treatment significantly slowed down tumor growth compared to control (p value = 0.005), moreover a transient reduction was observed with MET (p value = 0.03). After 3 weeks, only Combo-treated mice displayed tumor smaller than vehicle (p value = 0.04). Error bars indicate SEM. (D) T98G tumors from vehicle (CTRL), TMZ, MET or TMZ/metformin (Combo) treated mice, were analyzed for Ki67, Nestin and CD133 markers expression by immunohistochemistry. No difference between the tumor edge and bulk areas in IHC staining intensities or percentages of positive cells was observed for any of the investigated markers. Hematoxylin and eosin staining (H&E) was performed for morphological evaluation of the tumors. Representative images are shown with Original magnification x200. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23028), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neural stem cells could proliferate and differentiate into neurons and astrocytes.(A) Representative photomicrograph of neurospheres in culture. (B) Immunocytochemical staining of purified NSCs with Nestin. (C) Immunocytochemical staining of purified protoplasmic astrocytes with GFAP. (D) Immunocytochemical staining of purified neurons with beta -tubulin-III. (E) Nucleus staining of differentiated cells from NSCs with DAPI. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26431046), licensed under a CC-BY license. Not internally tested by R&D Systems.

miR–381 promoted neural stem cells proliferation.(A) The expression of miR–381 was measured by qRT-PCR. (B) CCK–8 was performed to detect the neural stem cells proliferation. (C) The mRNA expression of nestin was detected by qRT-PCR. (D) The protein expression of nestin was measured by Western blot. **p<0.01 and ***p<0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26431046), licensed under a CC-BY license. Not internally tested by R&D Systems.

Dissimilar tumorigenesis in the in vivo animal model.(a) Kaplan-Meier survival plots; 3 mice were used for cells of each clone. (b) Representative brain tumors of NOD-SCID mice harboring xenografts of the clones #2 (upper left), #3 (lower left), #4 (upper right) and #5 (lower right); H.E. staining, arrows: tumor, scale bar = 5 mm. (c) A representative xenograft of the clone #4; nestin-positive cells infiltrated the contralateral hemisphere (arrows). Scale bars = 5 mm (left), 1 mm (middle), 50 μm (right). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25623281), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cardiolipin is externalized to the mitochondrial surface in alpha -syn mutant hNs and transgenic mice in response to alpha -syn accumulation. a Western analysis of lysates from corrected and A53T cells at DIV 14 and DIV 60 labeled for total alpha -syn, PS129, TH (DA neuronal marker), nestin (NPC marker) or GAPDH show that levels of PS129-modified alpha -syn are elevated in A53T hNs at DIV 14 and remain elevated at DIV 60 relative to corrected cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29483518), licensed under a CC-BY license. Not internally tested by R&D Systems.

Validation of the phosphorylation status regarding the representative phosphoproteins by western blot analyses.Glioblastoma initiating cells were treated with 20 ng/ml EGF for 15 min and subjected to immunoblotting with the corresponding antibodies. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22912867), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clone: 196908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nestin Antibody (196908) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human Nestin
• Specificity: Detects human Nestin.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NES
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908) [Unconjugated]

• FLJ21841
• NES
• Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).

1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
2. Lendahl, U. et al. (1990) Cell 60:585.
3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
6. Frederiksen, K. et al. (1988) Neuron 1:439.
7. Cattaneo, E. et al. (1990) Nature 347:762.
8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
9. Rietze, R.L. et al. (2001) Nature 412:736.
10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
11. Zulewski, H. et al. (2001) Diabetes 50:521.
12. Lumelsky, N. et al. (2001) Science 292:1389.
13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nestin Antibody (MAB1259)(164)

Publications using MAB1259

• Andersen, MS;Halle, B;Wirenfeldt, M;Petersen, JK;Møller, MW;Jurmeister, P;Olsen, BB;Kristensen, BW;Boldt, H;Pedersen, CB;Mathiesen, T;Poulsen, FR; Orthotopic meningioma rat model exhibits morphological and immunohistochemical congruency and epigenetic concordance with benign primary patient-derived tumors Scientific reports 2024-12-30 [PMID: 39738335] (Immunohistochemistry, Human)
• Alexanian, AR;Sorokin, A;Duersteler, M; Dopaminergic progenitors generated by small molecule approach survived, integrated, and promoted functional recovery in (6-OHDA) mouse model of Parkinson's disease Journal of the neurological sciences 2024-08-20 [PMID: 39178824] (Immunocytochemistry, Human)
• Gupta, R;Dittmeier, M;Wohlleben, G;Nickl, V;Bischler, T;Luzak, V;Wegat, V;Doll, D;Sodmann, A;Bady, E;Langlhofer, G;Wachter, B;Havlicek, S;Gupta, J;Horn, E;Lüningschrör, P;Villmann, C;Polat, B;Wischhusen, J;Monoranu, CM;Kuper, J;Blum, R; Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene Cancer gene therapy 2024-07-22 [PMID: 39039193] (Immunohistochemistry, Human)
• Juchem, M;Lehmann, N;Behrens, YL;Bär, C;Thum, T;Hoepfner, J; CRISPR/Cas9-based GLA knockout to generate the female Fabry disease human induced pluripotent stem cell line MHHi001-A-15 Stem cell research 2024-06-20 [PMID: 38905814] (Flow Cytometry, Human)
• Jahn, C;Juchem, M;Sonnenschein, K;Gietz, A;Buchegger, T;Lachmann, N;Göhring, G;Behrens, YL;Bär, C;Thum, T;Hoepfner, J; Generation of human induced pluripotent stem cell line MHHi029-A from a male Fabry disease patient carrying c.959A &gt; T mutation Stem cell research 2024-03-24 [PMID: 38552356] (Flow Cytometry, Immunocytochemistry, Human)
• Frenz-Wiessner, S;Fairley, SD;Buser, M;Goek, I;Salewskij, K;Jonsson, G;Illig, D;Zu Putlitz, B;Petersheim, D;Li, Y;Chen, PH;Kalauz, M;Conca, R;Sterr, M;Geuder, J;Mizoguchi, Y;Megens, RTA;Linder, MI;Kotlarz, D;Rudelius, M;Penninger, JM;Marr, C;Klein, C; Generation of complex bone marrow organoids from human induced pluripotent stem cells Nature methods 2024-02-19 [PMID: 38374263] (Immunohistochemistry, Human)
• Bhatia, P;Bickle, M;Agrawal, A;Truss, B;Nikolaidi, A;Brockmann, K;Reinhardt, L;Vogel, S;Szegoe, E;Pal, A;Hermann, A;Miki?i?, I;Yun, M;Falkenburger, B;Sterneckert, J; Axonal Lysosomal Assays for Characterizing the Effects of LRRK2 G2019S Biology 2024-01-20 [PMID: 38275734] (Immunocytochemistry, Human)
• Gao, L;Behrens, A;Rodschinka, G;Forcelloni, S;Wani, S;Strasser, K;Nedialkova, DD; Selective gene expression maintains human tRNA anticodon pools during differentiation Nature cell biology 2024-01-08 [PMID: 38191669] (Immunocytochemistry, Human)
• Zimmer, N;Trzeciak, ER;Müller, A;Licht, P;Sprang, B;Leukel, P;Mailänder, V;Sommer, C;Ringel, F;Tuettenberg, J;Kim, E;Tuettenberg, A; Nuclear Glycoprotein A Repetitions Predominant (GARP) Is a Common Trait of Glioblastoma Stem-like Cells and Correlates with Poor Survival in Glioblastoma Patients Cancers 2023-12-05 [PMID: 38136258] (IHC, Xenograft)
• Patrycja Mulica, Carmen Venegas, Zied Landoulsi, Katja Badanjak, Sylvie Delcambre, Maria Tziortziou et al. Comparison of two protocols for the generation of iPSC-derived human astrocytes Biol Proced Online 2023-09-20 [PMID: 37730545]
• Michael W. Weible Ii, Michael D. Lovelace, Hamish D. Mundell, Tsz Wai Rosita Pang, Tailoi Chan-Ling BMPRII+ neural precursor cells isolated and characterized from organotypic neurospheres: an in vitro model of human fetal spinal cord development Neural Regeneration Research 2024-02-01 [PMID: 37488910] (Immunocytochemistry, Human)
• Garrudo, FFF;Filippone, G;Resina, L;Silva, JC;Barbosa, F;Ferreira, LFV;Esteves, T;Marques, AC;Morgado, J;Ferreira, FC; Production of Blended Poly(acrylonitrile): Poly(ethylenedioxythiophene):Poly(styrene sulfonate) Electrospun Fibers for Neural Applications Polymers 2023-06-21 [PMID: 37447406] (Human)
• Jia-jun Qiu, Yan-na Liu, Hao Wei, Fanyi Zeng, Jing-bin Yan Single-cell RNA sequencing of neural stem cells derived from human trisomic iPSCs reveals the abnormalities during neural differentiation of Down syndrome Frontiers in Molecular Neuroscience 6/15/2023 [PMID: 37396785]
• Tam, KW;Wong, CY;Wu, KL;Lam, G;Liang, X;Wong, WT;Li, MT;Liu, WY;Cai, S;Shea, GK;Shum, DK;Chan, YS; IPSC-Derived Sensory Neurons Directing Fate Commitment of Human BMSC-Derived Schwann Cells: Applications in Traumatic Neural Injuries Cells 2023-05-25 [PMID: 37296600] (Immunocytochemistry, Human)
• Dell'Amico, C;Angulo Salavarria, MM;Takeo, Y;Saotome, I;Dell'Anno, MT;Galimberti, M;Pellegrino, E;Cattaneo, E;Louvi, A;Onorati, M; Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors eLife 2023-06-05 [PMID: 37272619] (ICC, Human)
• Karapurkar JK, Kim MS, Colaco JC et al. CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis Journal of experimental & clinical cancer research : CR 2023-05-12 [PMID: 37170124]
  
  Details:
  IHC-P 1:100
• KN Eigenhuis, HB Somsen, M van der Kr, H Smeenk, AL Korporaal, SA Kushner, FMS de Vrij, DLC van den Be A simplified protocol for the generation of cortical brain organoids Frontiers in Cellular Neuroscience, 2023-04-04;17(0):1114420. 2023-04-04 [PMID: 37082206] (ICC, Human)
• G Rastoldo, I Watabe, A Lapotre, A Tonetto, A López-Juár, B Tighilet Vestibular Nuclei: A New Neural Stem Cell Niche? Cells, 2022-11-14;11(22):. 2022-11-14 [PMID: 36429025] (IHC, Rat)
• EJ Kim, JY Kim, SO Kim, N Hong, SH Choi, MG Park, J Jang, SW Ham, S Seo, SY Lee, K Lee, HJ Jeong, SJ Kim, S Jeong, K Min, SC Kim, X Jin, SH Kim, SH Kim, H Kim The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2 Cell Reports, 2022-11-22;41(8):111626. 2022-11-22 [PMID: 36417870] (Western Blot, Human)
• Wischhof L, Lee HM, Tutas J et al. BCL7A-containing SWI/SNF/BAF complexes modulate mitochondrial bioenergetics during neural progenitor differentiation The EMBO Journal 2022-12-01 [PMID: 36305367]
• Jing-Jing Sun, Qin Cai, Miao Xu, Yan-Na Liu, Wan-Rui Li, Juan Li et al. Loss of Protein Function Causing Severe Phenotypes of Female-Restricted Wieacker Wolff Syndrome due to a Novel Nonsense Mutation in the ZC4H2 Gene Genes (Basel) 8/29/2022 [PMID: 36140726]
• L Adnani, J Kassouf, B Meehan, C Spinelli, N Tawil, I Nakano, J Rak Angiocrine extracellular vesicles impose mesenchymal reprogramming upon proneural glioma stem cells Nature Communications, 2022-09-19;13(1):5494. 2022-09-19 [PMID: 36123372] (IHC, Human)
• J Brás, D Henriques, R Moreira, MM Santana, R Silva-Pedr, D Adão, S Braz, AR Álvaro, LP de Almeida, LS Mendonça Establishment and characterization of human pluripotent stem cells-derived brain organoids to model cerebellar diseases Scientific Reports, 2022-07-22;12(1):12513. 2022-07-22 [PMID: 35869235] (IHC, Human)
• RR Khadim, RK Vadivelu, T Utami, FG Torizal, M Nishikawa, Y Sakai Integrating Oxygen and 3D Cell Culture System: A Simple Tool to Elucidate the Cell Fate Decision of hiPSCs International Journal of Molecular Sciences, 2022-06-30;23(13):. 2022-06-30 [PMID: 35806277] (ICC, Human)
• YH Chen, PS Huang, MH Wen, M Pan, DF Lee, TY Chen Generation of a homozygous knock-in human embryonic stem cell line expressing mEos4b-tagged CTR1 Oncogene, 2022-06-14;63(0):102845. 2022-06-14 [PMID: 35728441] (ICC, Human)
• Elena Fdez, Jesús Madero-Pérez, Antonio J. Lara Ordóñez, Yahaira Naaldijk, Rachel Fasiczka, Ana Aiastui et al. Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement iScience 2022-06-17 [PMID: 35721463] (Immunocytochemistry, Human)
• Elisa Degl’Innocenti, Tino Emanuele Poloni, Valentina Medici, Luca Recupero, Claudia Dell’Amico, Eleonora Vannini et al. Centrin 2: A Novel Marker of Mature and Neoplastic Human Astrocytes Frontiers in Cellular Neuroscience 2022-04-29 [PMID: 35573835]
• Fabio Bertan, Lena Wischhof, Enzo Scifo, Mihaela Guranda, Joshua Jackson, Anaïs Marsal-Cots et al. Comparative analysis of CI- and CIV-containing respiratory supercomplexes at single-cell resolution Cell Reports Methods 2021-05-24 [PMID: 35474694]
• L Schwarz, JC Fitzgerald Steady-State Levels of Miro1 Linked to Phosphorylation at Serine 156 and Mitochondrial Respiration in Dopaminergic Neurons Cells, 2022-04-08;11(8):. 2022-04-08 [PMID: 35455950] (ICC, Human)
• M Tisch, M Carmen De, M Suarez-Cub, C Fauth, M Defrancesc, J Zschocke, K Günther, F Edenhofer Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene Stem Cell Research, 2022-04-09;61(0):102784. 2022-04-09 [PMID: 35453044] (ICC, Human)
• YW Cho, S Jee, IR Suhito, JH Lee, CG Park, KM Choi, TH Kim Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiation Science Advances, 2022-04-20;8(16):eabj7736. 2022-04-20 [PMID: 35442746] (ICC, Human)
• Aida Amador-Álvarez, María A. Gómez-Muñoz, Ismael Rodríguez-Prieto, Ricardo Pardal, Francisco M. Vega A protocol to enrich in undifferentiated cells from neuroblastoma tumor tissue samples and cell lines STAR Protocols 2022-06-01 [PMID: 35345594]
• K Kaushal, EJ Kim, A Tyagi, JK Karapurkar, S Haq, HS Jung, KS Kim, S Ramakrishn Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis Cell Death and Differentiation, 2022-03-10;0(0):. 2022-03-10 [PMID: 35273362] (ICC, Human)
• B Polat, G Wohlleben, R Kosmala, D Lisowski, F Mantel, V Lewitzki, M Löhr, R Blum, P Herud, M Flentje, CM Monoranu Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma Cancer Cell International, 2022-02-19;22(1):87. 2022-02-19 [PMID: 35183162] (IF, IHC, Human)
• B Bohl, A Jabali, J Ladewig, P Koch Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells Science Advances, 2022-02-11;8(6):eabl5792. 2022-02-11 [PMID: 35148180] (ICC, Human)
• You Y, Muraoka S, Jedrychowski MP et al. Human neural cell type-specific�extracellular vesicle proteome defines disease-related molecules associated with activated astrocytes in Alzheimer's disease brain Journal of Extracellular Vesicles 2022-01-13 [PMID: 35029059]
• Adrian Odrzywolski, Bożena Jarosz, Michał Kiełbus, Ilona Telejko, Dominik Ziemianek, Sebastian Knaga et al. Profiling Glioblastoma Cases with an Expression of DCX, OLIG2 and NES International Journal of Molecular Sciences 2021-12-08 [PMID: 34948016]
• PA Barbuti, J Ohnmacht, BFR Santos, PM Antony, F Massart, G Cruciani, CM Dording, L Pavelka, N Casadei, YJ Kwon, R Krüger Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function Scientific Reports, 2021-11-09;11(1):21946. 2021-11-09 [PMID: 34754035] (IHC, Human)
• Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 2021-10-22;57(0):102583. 2021-10-22 [PMID: 34710837] (Flow Cytometry, ICC, Human)
• J Gil-Ranedo, C Gallego-Ga, JM Almendral Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations Cell Reports, 2021-09-07;36(10):109673. 2021-09-07 [PMID: 34496248] (ICC, Human)
• Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks Rapid target validation in a Cas9-inducible hiPSC derived kidney model Scientific Reports, 2021-08-16;11(1):16532. 2021-08-16 [PMID: 34400685] (IHC, Human)
• S Rajasingh, V Sigamani, V Selvam, N Gurusamy, S Kirankumar, J Vasanthan, J Rajasingh Comparative analysis of human induced pluripotent stem cell-derived mesenchymal stem cells and umbilical cord mesenchymal stem cells Journal of Cellular and Molecular Medicine, 2021-08-13;0(0):. 2021-08-13 [PMID: 34390186] (ICC, Human)
• F Xiong, R Wang, JH Lee, S Li, SF Chen, Z Liao, LA Hasani, PT Nguyen, X Zhu, J Krakowiak, DF Lee, L Han, KL Tsai, Y Liu, W Li RNA m6A modification orchestrates a LINE-1-host interaction that facilitates retrotransposition and contributes to long gene vulnerability Cell Research, 2021-06-09;0(0):. 2021-06-09 [PMID: 34108665] (ICC, Human)
• H Walgrave, S Balusu, S Snoeck, E Vanden Eyn, K Craessaert, N Thrupp, L Wolfs, K Horré, Y Fourne, A Ronisz, E Silajdži?, A Penning, G Tosoni, Z Callaerts-, R D'Hooge, DR Thal, H Zetterberg, S Thuret, M Fiers, CS Frigerio, B De Stroope, E Salta Restoring miR-132 expression rescues adult hippocampal neurogenesis and memory deficits in Alzheimer&#039;s disease Cell Stem Cell, 2021-05-24;0(0):. 2021-05-24 [PMID: 34033742] (ICC, Human)
• MJ Ruiz-Magañ, R Martinez-A, T Llorca, AC Abadia-Mol, C Ruiz-Ruiz, EG Olivares Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity Journal of reproductive immunology, 2021-05-01;145(0):103326. 2021-05-01 [PMID: 33965695] (Flow Cytometry, IHC, Human)
• Ryutaro Kajihara, Tadahiro Numakawa, Takumi Era Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs) BIO-PROTOCOL 2021-02-05 [PMID: 33732801] (Immunocytochemistry, Human)
• Elena Morelli, Elisa A. Speranza, Enrica Pellegrino, Galina V. Beznoussenko, Francesca Carminati, Massimiliano Garré et al. Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus Frontiers in Cell and Developmental Biology 2021-02-18 [PMID: 33718375]
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Reviews for Nestin Antibody (MAB1259) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 02/10/2022

Summary

• Application: Immunocytochemistry
• Sample Tested: Neural stem cells
• Species: Human
• Lot: 196908

reviewed by: Verified Customer Immunofluorescence - paraffin Human 03/09/2019

Summary

• Application: Other
• Sample Tested: human glioblastoma
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH9 heat induced antigen retrieval

reviewed by: Verified Customer IF Human 02/04/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 24213561
• Species: Human

Bioinformatics

• Gene Symbol: NES