Nestin Antibody (196908)

Images

 
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-reported, Flow, ICC/IF
Clone
196908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Nestin Antibody (196908) Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Specificity
Detects human Nestin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
MAB1259 in the following applications:

Publications
Read Publications using
MAB1259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908)

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (MAB1259)(53)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 14 applications: Flow, ICC, ICC Fluorescence, ICC Frozen, IF, IHC, IHC Frozen, IHC - Fresh, IHC - Paraffin embedded, IHC Frozen, IHC Paraffin-embedded, IHC paraffin embedded, IHC-P, WB.


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Flow
(5)
ICC
(29)
ICC Fluorescence
(1)
ICC Frozen
(1)
IF
(1)
IHC
(1)
IHC Frozen
(1)
IHC - Fresh
(1)
IHC - Paraffin embedded
(2)
IHC Frozen
(1)
IHC Paraffin-embedded
(3)
IHC paraffin embedded
(1)
IHC-P
(1)
WB
(4)
All Applications
Filter By Species
Human
(48)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using MAB1259 Applications Species
R Tirughana, MZ Metz, Z Li, C Hall, D Hsu, J Beltzer, AJ Annala, D Oganesyan, M Gutova, KS Aboody GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System Mol Ther Methods Clin Dev, 2018;10(0):48-56. 2018 [PMID: 29992178] (Flow,, Human) Flow Human
S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime J Transl Med, 2018;16(1):148. 2018 [PMID: 29859097] (IHC-P,, Human) IHC-P Human
L Marrone, C Bus, D Schöndorf, JC Fitzgerald, M Kübler, B Schmid, P Reinhardt, L Reinhardt, M Deleidi, T Levin, A Meixner, B Klink, M Glatza, CJ Gloeckner, T Gasser, J Sternecker Generation of iPSCs carrying a common LRRK2 risk allele for in vitro modeling of idiopathic Parkinson's disease PLoS ONE, 2018;13(3):e0192497. 2018 [PMID: 29513666] (ICC,, Human) ICC Human
F Hsu, S Spannl, C Ferguson, AA Hyman, RG Parton, M Zerial Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria Elife, 2018;7(0):. 2018 [PMID: 29469808] (ICC,, Mouse) ICC Mouse
D Ghoubay-Be, C de Sousa, R Martos, G Latour, MC Schanne-Kl, E Dupin, V Borderie Easy xeno-free and feeder-free method for isolating and growing limbal stromal and epithelial stem cells of the human cornea PLoS ONE, 2017;12(11):e0188398. 2017 [PMID: 29149196] (ICC,, Human) ICC Human
F Wang, AY Wang, C Chesnelong, Y Yang, A Nabbi, S Thalappill, V Alekseev, K Riabowol ING5 activity in self-renewal of glioblastoma stem cells via calcium and follicle stimulating hormone pathways Oncogene, 2018;37(3):286-301. 2018 [PMID: 28925404] (ICC,, Human) ICC Human
AM Rosager, MD Sørensen, RH Dahlrot, S Hansen, DL Schonberg, JN Rich, JD Lathia, BW Kristensen Transferrin receptor-1 and ferritin heavy and light chains in astrocytic brain tumors: Expression and prognostic value PLoS ONE, 2017;12(8):e0182954. 2017 [PMID: 28837569] (IHC paraffin embedded,, Human) IHC paraffin embedded Human
R Kumar, A Gont, TJ Perkins, JEL Hanson, IAJ Lorimer Induction of senescence in primary glioblastoma cells by serum and TGF? Sci Rep, 2017;7(1):2156. 2017 [PMID: 28526854] (ICC,, Human) ICC Human
X Yuan, Z Li, AC Baines, E Gavriilaki, Z Ye, Z Wen, EM Braunstein, LG Biesecker, L Cheng, X Dong, RA Brodsky A hypomorphic PIGA gene mutation causes severe defects in neuron development and susceptibility to complement-mediated toxicity in a human iPSC model PLoS ONE, 2017;12(4):e0174074. 2017 [PMID: 28441409] (ICC,, Human) ICC Human
P Wang, C Lan, S Xiong, X Zhao, Y Shan, R Hu, W Wan, S Yu, B Liao, G Li, J Wang, D Zou, B Chen, H Feng, N Wu HIF1? regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia Oncotarget, 2017;8(17):28074-28092. 2017 [PMID: 28427209] (WB,, Human) WB Human
Show All 53 Publications.

Review for Nestin Antibody (MAB1259) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1259:
Filter by Applications
IF
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Human 02/04/2015
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Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24213561
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24213561

Details

Detection NotesSee PMID 24213561

Comments

CommentsIndependent review from antibodyresource.com

FAQs for Nestin Antibody (MAB1259) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Bioinformatics Tool for Nestin Antibody (MAB1259)

Discover related pathways, diseases and genes to Nestin Antibody (MAB1259). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Nestin Antibody (MAB1259)

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Blogs on Nestin.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Recent Reviews

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Anonymous
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NES
Entrez