Nestin Antibody (196908)

Images

 
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-reported, Flow, ICC/IF
Clone
196908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Nestin Antibody (196908) Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Specificity
Detects human Nestin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 3 Reviews rated 4.3
using
MAB1259 in the following applications:

Publications
Read Publications using
MAB1259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908)

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (MAB1259)(94)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 8 applications: Bioassay, Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(8)
ICC
(55)
IF
(2)
IHC
(9)
IHC-Fr
(4)
IHC-P
(7)
Western Blot
(7)
All Applications
Filter By Species
Human
(85)
Mouse
(7)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 94. Show All 94 Publications.
Publications using MAB1259 Applications Species
L Schwarz, JC Fitzgerald Steady-State Levels of Miro1 Linked to Phosphorylation at Serine 156 and Mitochondrial Respiration in Dopaminergic Neurons Cells, 2022;11(8):. 2022 [PMID: 35455950] (ICC, Human) ICC Human
M Tisch, M Carmen De, M Suarez-Cub, C Fauth, M Defrancesc, J Zschocke, K Günther, F Edenhofer Generation of the human induced pluripotent stem cell line (IBKMOLi002-A) from PBMCs of a patient carrying the heterozygous L271H mutation of the voltage-gated calcium channel subunit Cav1.3-encoding CACNA1D gene Stem Cell Research, 2022;61(0):102784. 2022 [PMID: 35453044] (ICC, Human) ICC Human
YW Cho, S Jee, IR Suhito, JH Lee, CG Park, KM Choi, TH Kim Single metal-organic framework-embedded nanopit arrays: A new way to control neural stem cell differentiation Science Advances, 2022;8(16):eabj7736. 2022 [PMID: 35442746] (ICC, Human) ICC Human
K Kaushal, EJ Kim, A Tyagi, JK Karapurkar, S Haq, HS Jung, KS Kim, S Ramakrishn Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis Cell Death and Differentiation, 2022;0(0):. 2022 [PMID: 35273362] (ICC, Human) ICC Human
B Polat, G Wohlleben, R Kosmala, D Lisowski, F Mantel, V Lewitzki, M Löhr, R Blum, P Herud, M Flentje, CM Monoranu Differences in stem cell marker and osteopontin expression in primary and recurrent glioblastoma Cancer Cell International, 2022;22(1):87. 2022 [PMID: 35183162] (IF, Human) IF Human
B Bohl, A Jabali, J Ladewig, P Koch Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells Science Advances, 2022;8(6):eabl5792. 2022 [PMID: 35148180] (ICC, Human) ICC Human
PA Barbuti, J Ohnmacht, BFR Santos, PM Antony, F Massart, G Cruciani, CM Dording, L Pavelka, N Casadei, YJ Kwon, R Krüger Gene-corrected p.A30P SNCA patient-derived isogenic neurons rescue neuronal branching and function Scientific Reports, 2021;11(1):21946. 2021 [PMID: 34754035] (IHC, Human) IHC Human
Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 2021;57(0):102583. 2021 [PMID: 34710837] (Flow Cytometry, Human) Flow Cytometry Human
J Gil-Ranedo, C Gallego-Ga, JM Almendral Viral targeting of glioblastoma stem cells with patient-specific genetic and post-translational p53 deregulations Cell Reports, 2021;36(10):109673. 2021 [PMID: 34496248] (ICC, Human) ICC Human
Y Shamshirga, A Jonebring, A Svensson, I Leefa, M Bohlooly-Y, M Firth, KJ Woollard, A Hofherr, IM Rogers, R Hicks Rapid target validation in a Cas9-inducible hiPSC derived kidney model Scientific Reports, 2021;11(1):16532. 2021 [PMID: 34400685] (IHC, Human) IHC Human
Show All 94 Publications.

Reviews for Nestin Antibody (MAB1259) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using MAB1259:
Filter by Applications
ICC
(1)
Immunofluorescence - paraffin
(1)
IF
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Nestin MAB1259
Enlarge
5
reviewed by:
Layla Schwartz
ICC Human 02/10/2022
View

Summary

ApplicationImmunocytochemistry
Sample TestedNeural stem cells
SpeciesHuman
Lot196908
Other Nestin MAB1259
Enlarge
4
reviewed by:
Christian Schuerch
Immunofluorescence - paraffin Human 03/09/2019
View

Summary

ApplicationOther
Sample Testedhuman glioblastoma
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 heat induced antigen retrieval
  4
reviewed by:
cynthia morimoto
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24213561
SpeciesHuman

FAQs for Nestin Antibody (MAB1259) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Bioinformatics Tool for Nestin Antibody (MAB1259)

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Layla Schwartz
02/10/2022
Application: ICC
Species: Human

Christian Schuerch
03/09/2019
Application: Immunofluorescence - paraffin
Species: Human

 
cynthia morimoto
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NES