Nestin Antibody (196908)

Images

 
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications CyTOF-reported, Flow, ICC/IF
Clone
196908
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Nestin Antibody (196908) Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Nestin
Specificity
Detects human Nestin.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NES
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-reported
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 2 Reviews rated 4
using
MAB1259 in the following applications:

Publications
Read Publications using
MAB1259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nestin Antibody (196908)

  • FLJ21841
  • NES
  • Nestin

Background

Nestin is a class VI intermediate filament protein (1, 2) that is expressed in stem cells of the central nervous system (CNS) (3) but not in mature CNS cells (4). Nestin expression is used extensively as a marker for CNS stem cells in the developing nervous system and in vitro cultured cells (5-10). Its transient expression is a critical step in the neural differentiation pathway (2). Nestin is also expressed in non-neural stem cell populations, such as pancreatic islet progenitors (11-13) and hematopoietic progenitors (14).
  1. Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
  2. Lendahl, U. et al. (1990) Cell 60:585.
  3. Frederiksen, K. and R.D. McKay (1988) J. Neurosci. 8:1144.
  4. Tohyama, T. et. al. (1992) Lab. Invest. 66:303.
  5. Uchida, N. et al. (2000) Proc. Natl. Acad.Sci. USA 97:14720.
  6. Frederiksen, K. et al. (1988) Neuron 1:439.
  7. Cattaneo, E. et al. (1990) Nature 347:762.
  8. Reynolds, B.A. and S. Weiss (1992) Science 255:1707.
  9. Rietze, R.L. et al. (2001) Nature 412:736.
  10. Carpenter, M.K. et al. (2001) Exp. Neurol 172:383.
  11. Zulewski, H. et al. (2001) Diabetes 50:521.
  12. Lumelsky, N. et al. (2001) Science 292:1389.
  13. Lechner, A. et al. (2002) Biochem.Biophys. Res. Commun. 293:670.
  14. Shih, C.C. et al. (2001) Blood 98:2412.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nestin Antibody (MAB1259)(65)

We have publications tested in 3 confirmed species: Human, Mouse, Xenograft.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(6)
ICC
(38)
IHC
(5)
IHC-Fr
(4)
IHC-P
(7)
Western Blot
(4)
All Applications
Filter By Species
Human
(58)
Mouse
(5)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using MAB1259 Applications Species
S Ross, M Holliday, S Lim, C Semsarian Characterization of the first induced pluripotent stem cell line generated from a patient with autosomal dominant catecholaminergic polymorphic ventricular tachycardia due to a heterozygous mutation in cardiac calsequestrin-2 Stem Cell Res, 2019;37(0):101450. 2019 [PMID: 31039485] (ICC, Human) ICC Human
C Zalfa, L Rota Nodar, E Vacchi, M Gelati, D Profico, M Boido, E Binda, L De Filippi, M Copetti, V Garlatti, P Daniele, J Rosati, A De Luca, F Pinos, L Cajola, A Visioli, L Mazzini, A Vercelli, M Svelto, AL Vescovi, D Ferrari Transplantation of clinical-grade human neural stem cells reduces neuroinflammation, prolongs survival and delays disease progression in the SOD1 rats Cell Death Dis, 2019;10(5):345. 2019 [PMID: 31024007] (IHC, Xenograft) IHC Xenograft
J Luzuriaga, O Pastor-Alo, JM Encinas, F Unda, G Ibarretxe, JR Pineda Human Dental Pulp Stem Cells Grown in Neurogenic Media Differentiate Into Endothelial Cells and Promote Neovasculogenesis in the Mouse Brain Front Physiol, 2019;10(0):347. 2019 [PMID: 30984027] (IHC-Fr, Mouse) IHC-Fr Mouse
BW Stringer, BW Day, RCJ D'Souza, PR Jamieson, KS Ensbey, ZC Bruce, YC Lim, K Goasdoué, C Offenhäuse, S Akgül, S Allan, T Robertson, P Lucas, G Tollesson, S Campbell, C Winter, H Do, A Dobrovic, PL Inglis, RL Jeffree, TG Johns, AW Boyd A reference collection of patient-derived cell line and xenograft models of proneural, classical and mesenchymal glioblastoma Sci Rep, 2019;9(1):4902. 2019 [PMID: 30894629] (Flow Cytometry, Human) Flow Cytometry Human
I Bertolini, A Terrasi, C Martelli, G Gaudioso, A Di Cristof, AM Storaci, M Formica, P Braidotti, K Todoerti, S Ferrero, M Caroli, L Ottobrini, T Vaccari, V Vaira A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes EBioMedicine, 2019;0(0):. 2019 [PMID: 30737083] (IHC, Human) IHC Human
M Holliday, SB Ross, S Lim, C Semsarian Generation of an induced pluripotent stem cell line from a hypertrophic cardiomyopathy patient with a pathogenic myosin binding protein C (MYBPC3) p.Arg502Trp mutation Stem Cell Res, 2018;33(0):56-59. 2018 [PMID: 30316040] (ICC, Human) ICC Human
CP Beier, T Rasmussen, RH Dahlrot, HB Tenstad, JS Aarø, MF Sørensen, SB Heimisdótt, MD Sørensen, P Svenningse, MJ Riemenschn, D Beier, BW Kristensen Aberrant neuronal differentiation is common in glioma but is associated neither with epileptic seizures nor with better survival Sci Rep, 2018;8(1):14965. 2018 [PMID: 30297697] (ICC, Human) ICC Human
J Rosati, D Ferrari, F Altieri, S Tardivo, C Ricciolini, C Fusilli, C Zalfa, DC Profico, F Pinos, L Bernardini, B Torres, I Manni, G Piaggio, E Binda, M Copetti, G Lamorte, T Mazza, M Carella, M Gelati, EM Valente, A Simeone, AL Vescovi Establishment of stable iPS-derived human neural stem cell lines suitable for cell therapies Cell Death Dis, 2018;9(10):937. 2018 [PMID: 30224709] (IHC, Human) IHC Human
R Tirughana, MZ Metz, Z Li, C Hall, D Hsu, J Beltzer, AJ Annala, D Oganesyan, M Gutova, KS Aboody GMP Production and Scale-Up of Adherent Neural Stem Cells with a Quantum Cell Expansion System Mol Ther Methods Clin Dev, 2018;10(0):48-56. 2018 [PMID: 29992178] (Flow Cytometry, Human) Flow Cytometry Human
S Moll, Y Yasui, A Abed, T Murata, H Shimada, A Maeda, N Fukushima, M Kanamori, S Uhles, L Badi, T Cagarelli, I Formentini, F Drawnel, G Georges, T Bergauer, R Gasser, RD Bonfil, R Fridman, H Richter, J Funk, MJ Moeller, C Chatzianto, M Prunotto Selective pharmacological inhibition of DDR1 prevents experimentally-induced glomerulonephritis in prevention and therapeutic regime J Transl Med, 2018;16(1):148. 2018 [PMID: 29859097] (IHC-P, Human) IHC-P Human
Show All 65 Publications.

Reviews for Nestin Antibody (MAB1259) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using MAB1259:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 Nestin MAB1259
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reviewed by:
Anonymous
Human 03/09/2019
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Summary

Sample Testedhuman glioblastoma
SpeciesHuman
LotI don't have it any more

Comments

CommentspH9 heat induced antigen retrieval
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24213561
SpeciesHuman

Blocking

Blocking DetailsSee PMID 24213561

Details

Detection NotesSee PMID 24213561

Comments

CommentsIndependent review from antibodyresource.com

FAQs for Nestin Antibody (MAB1259) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

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Bioinformatics Tool for Nestin Antibody (MAB1259)

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Blogs on Nestin.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Nestin Antibody Products in Neuronal Cancer Research
Nestin is a large class Vl intermediate filament protein predominantly expressed in the neuroepithelial stem cells of the embryonic central nervous system, although recent nestin antibody studies have shown it expressed in other cell types, including...  Read full blog post.

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Recent Reviews

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5
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4
2
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2
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1
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Anonymous
03/09/2019
Application:
Species: Human

 
Anonymous
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol NES
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