Recombinant Human FGF-4 Protein


1 μg/lane of Recombinant Human FGF-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.
Recombinant Human FGF-4 (Catalog # 235‑F4) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.25‑1.25 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human FGF-4 Protein Summary

Additional Information
A New and Improved rhFGF-4 is Now Available! It has enhanced purity!
New Product 7460-F4
Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.25-1.25 ng/mL.
E. coli-derived human FGF-4 protein
Accession #
N-terminal Sequence
Ser54 & Leu55
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
16 kDa, reducing conditions
Read Publications using
235-F4 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-4 Protein

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST


FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

  1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  3. Niswander, L. and G.R. Martin (1992) Development 114:755.
  4. Feldman, B. et al. (1995) Science 267:246.
  5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
  6. Sun, X. et al. (2002) Nature 418:501.
  7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
  8. Yu, K and D.M. Ornitz (2008) Development 135:483.
  9. Mariani, F.V. et al. (2008) Nature 453:401.
  10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
  11. Kunath, T. et al. (2007) Development 134:2895.
  12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
  13. Hajitou, A. et al. (1998) Oncogene 17:2059.
  14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

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Publications for FGF-4 (235-F4)(35)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Porcine, Xenopus.

We have publications tested in 3 applications: Bioassay, Expansion/Differentiation, In Vivo.

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Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using 235-F4 Applications Species
R Okamoto, K Takayama, N Akita, Y Nagamoto, D Hosokawa, S Iizuka, F Sakurai, H Suemizu, K Ohashi, H Mizuguchi Human iPS Cell-based Liver-like Tissue Engineering at Extrahepatic Sites in Mice as a New Cell Therapy for Hemophilia B Cell Transplant, 2018;27(2):299-309. 2018 [PMID: 29637813] (Bioassay, Human) Bioassay Human
WS Fagg, N Liu, MJ Yang, K Cheng, E Chung, JS Kim, G Wu, J Fair Magnetic Targeting of Stem Cell Derivatives Enhances Hepatic Engraftment into Structurally Normal Liver Cell Transplant, 2017;26(12):1868-1877. 2017 [PMID: 29390880] (Bioassay, Mouse) Bioassay Mouse
JO Múnera, N Sundaram, SA Rankin, D Hill, C Watson, M Mahe, JE Vallance, NF Shroyer, KL Sinagoga, A Zarzoso-La, JR Hudson, JC Howell, P Chatuvedi, JR Spence, JM Shannon, AM Zorn, MA Helmrath, JM Wells Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling Cell Stem Cell, 2017;0(0):. 2017 [PMID: 28648364] (Bioassay, Human) Bioassay Human
M Kang, V Garg, AK Hadjantona Lineage Establishment and Progression within the Inner Cell Mass of the Mouse Blastocyst Requires FGFR1 and FGFR2 Dev. Cell, 2017;0(0):. 2017 [PMID: 28552559] (Bioassay, Mouse) Bioassay Mouse
AB Hanker, JG Garrett, MV Estrada, PD Moore, PG Ericsson, JP Koch, E Langley, S Singh, PS Kim, GM Frampton, EM Sanford, P Owns, J Becker, MR Groseclose, S Castellino, H Joensuu, J Huober, JC Brase, M Samira, S Brohée, D Venet, D Brown, J Baselga, M Piccart, C Sotiriou, CL Arteaga HER2-overexpressing breast cancers amplify FGFR signaling upon acquisition of resistance to dual therapeutic blockade of HER2 Clin. Cancer Res., 2017;0(0):. 2017 [PMID: 28381415] (Bioassay, Human) Bioassay Human
DA Elberry, SN Amin, RS Esmail, LA Rashed, MM Gamal Effect of undifferentiated versus hepatogenic partially differentiated mesenchymal stem cells on hepatic and cognitive functions in liver cirrhosis EXCLI J, 2016;15(0):652-670. 2016 [PMID: 28337098] (Bioassay, Rat) Bioassay Rat
DB Holmes, VM Heine Simplified 3D protocol capable of generating early cortical neuroepithelium Biol Open, 2017;0(0):. 2017 [PMID: 28167491] (Bioassay, Human) Bioassay Human
KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 2017;0(0):. 2017 [PMID: 28052057] (Bioassay, Human) Bioassay Human
Asynchronous fate decisions by single cells collectively ensure consistent lineage composition in the mouse blastocyst Nat Commun, 2016;7(0):13463. 2016 [PMID: 27857135] (Bioassay, Mouse) Bioassay Mouse
Growth hormone is permissive for neoplastic colon growth Proc Natl Acad Sci USA, 2016;0(0):. 2016 [PMID: 27226307] (Bioassay, Human) Bioassay Human
Show All 35 Publications.

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Gene Symbol FGF4