Recombinant Human FGF-4 Protein


1 μg/lane of Recombinant Human FGF-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 16 kDa.
Recombinant Human FGF-4 (Catalog # 235‑F4) stimulates cell proliferation of the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 0.25‑1.25 ng/mL.

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Applications Bioactivity

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Recombinant Human FGF-4 Protein Summary

Additional Information
A New and Improved rh FGF-4 is Now Available! It has enhanced purity!
Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.25-1.25 ng/mL.
E. coli-derived human FGF-4 protein
Accession #
N-terminal Sequence
Ser54 & Leu55
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
16 kDa, reducing conditions
Read Publications using
235-F4 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-4 Protein

  • FGF4
  • FGF-4
  • fibroblast growth factor 4
  • HBGF-4
  • HBGF-4Transforming protein KS3
  • heparin secretory transforming protein 1
  • Heparin secretory-transforming protein 1
  • Heparin-binding growth factor 4
  • HST-1
  • HST-1HSTF-1
  • HSTF1fibroblast growth factor 4 splice isoform
  • HSTFGF-4
  • human stomach cancer, transforming factor from FGF-related oncogene
  • kaposi sarcoma oncogene
  • KFGF
  • K-FGF
  • KS3
  • oncogene HST


FGF-4 (fibroblast growth factor-4), also known as FGF-K or K-FGF (Kaposi’s sarcoma-associated FGF), is a 25 kDa secreted, heparin-binding member of the FGF family (1, 2). The human FGF-4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus (2). Mature human FGF-4 (aa 71-206) shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF-4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development (1, 3). Its expression in the mouse trophoblast inner cell mass promotes expression of FGF R2, and is required for maintenance of the trophectoderm and primitive endoderm (3-5). Later in mouse development, FGF-4 works together with FGF-8 to mediate the activities of the apical ectodermal ridge, which direct the outgrowth and patterning of vertebrate limbs (3, 6-9). FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell self-renewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro (10-12). A C-terminally truncated 15 kDa isoform that opposes full-length FGF-4 and promotes differentiation is endogenously expressed in human embryonic stem cells. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential (13). It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease (14).

  1. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  2. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
  3. Niswander, L. and G.R. Martin (1992) Development 114:755.
  4. Feldman, B. et al. (1995) Science 267:246.
  5. Goldin, S.N. and V.E. Papaioannou (2003) Genesis 36:40.
  6. Sun, X. et al. (2002) Nature 418:501.
  7. Boulet, A.M. et al. (2004) Dev. Biol. 273:361.
  8. Yu, K and D.M. Ornitz (2008) Development 135:483.
  9. Mariani, F.V. et al. (2008) Nature 453:401.
  10. Johannesson, M. et al. (2009) PLoS ONE 4:e4794.
  11. Kunath, T. et al. (2007) Development 134:2895.
  12. Mayshar, Y. et al. (2008) Stem Cells 26:767.
  13. Hajitou, A. et al. (1998) Oncogene 17:2059.
  14. Flynn, A. and T. O’Brien (2008) IDrugs 11:283.

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Publications for FGF-4 (235-F4)(47)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Porcine, Xenopus.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

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Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using 235-F4 Applications Species
H Zhang, LTP Khoa, F Mao, H Xu, B Zhou, Y Han, M O'Leary, A Nusrat, L Wang, TL Saunders, Y Dou MLL1 Inhibition and Vitamin D Signaling Cooperate to Facilitate the Expanded Pluripotency State Cell Rep, 2019;29(9):2659-2671.e6. 2019 [PMID: 31775036] (Cell Culture, Mouse) Cell Culture Mouse
KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells J Clin Med, 2019;8(7):. 2019 [PMID: 31277507] (Bioassay, Human) Bioassay Human
KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells J Clin Med, 2019;8(7):. 2019 [PMID: 31277507] (Bioassay, Human) Bioassay Human
RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development Cell Rep, 2019;27(3):708-718.e10. 2019 [PMID: 30995470] (Bioassay, Human) Bioassay Human
Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi FGF signal is not required for hepatoblast differentiation of human iPS cells Sci Rep, 2019;9(1):3713. 2019 [PMID: 30842525] (Bioassay, Human) Bioassay Human
V Chesnokova, S Zonis, R Barrett, H Kameda, K Wawrowsky, A Ben-Shlomo, M Yamamoto, J Gleeson, C Bresee, V Gorbunova, S Melmed Excess growth hormone suppresses DNA damage repair in epithelial cells JCI Insight, 2019;4(3):. 2019 [PMID: 30728323] (Bioassay, Human) Bioassay Human
TR Broda, KW McCracken, JM Wells Generation of human antral and fundic gastric organoids from pluripotent stem cells Nat Protoc, 2019;14(1):28-50. 2019 [PMID: 30470820] (Bioassay, Human) Bioassay Human
A Hyder, S Ehnert, F Fändrich, H Ungefroren Transfection of Peripheral Blood Monocytes with SOX2 Enhances Multipotency, Proliferation, and Redifferentiation into Neohepatocytes and Insulin-Producing Cells Stem Cells Int, 2018;2018(0):4271875. 2018 [PMID: 30402109] (Bioassay, Human) Bioassay Human
B Wang, JH Joo, R Mount, BJW Teubner, A Krenzer, AL Ward, VP Ichhaporia, EJ Adams, R Khoriaty, ST Peters, SM Pruett-Mil, SS Zakharenko, D Ginsburg, M Kundu The COPII cargo adapter SEC24C is essential for neuronal homeostasis J. Clin. Invest., 2018;0(0):. 2018 [PMID: 29939162] (Bioassay, Human) Bioassay Human
MJ Workman, JP Gleeson, EJ Troisi, HQ Estrada, SJ Kerns, CD Hinojosa, GA Hamilton, SR Targan, CN Svendsen, RJ Barrett Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol, 2018;5(4):669-677.e2. 2018 [PMID: 29930984] (Bioassay, Human) Bioassay Human
Show All 47 Publications.

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Gene Symbol FGF4