SOX2: Novus Biologicals

AF2018

SOX2 Antibody [Unconjugated]

(6 Reviews)

100 μg

AF2018

HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR." class="big_lightbox">

BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (<a class=

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox">

SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; (<a class=

NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox">

SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_lightbox">

SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface and nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX2 was detected in immersion fixed NTera‑2 human testicular embryonic carcinoma cells (Positive) & absent in HepG2 human hepatocellular carcinoma cells (Negative) using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to Cell surface and nuclear. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

<b>Genetic Strategies Validation. </b>Knockdown OGT inhibits proliferation and tumorsphere formation of hepatoma cell through reducing eIF4E expression. A, Huh7 and PLC/PRF/5 cells were infected with control shRNA, OGT shRNA2 alone, or with wild‐type eIF4E lentivirus. The cell lysates were harvested for western blotting analysis using indicated antibodies. beta ‐actin expression was served as a loading control. B, Cell proliferation of Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were measured with CCK8 assay. (C‐H) Huh7 and PLC/PRF/5 cells infected with lentiviruses as in panel (A) were seeded into 96‐well plates. After 12 d, tumorsphere were counted and quantified. Representative images of sphere (scale bars, 100 μm) were shown (C, F). The diameter of sphere (D, G) and number of sphere (E, H) were count. Data represent mean ± SD of at least three independent experiments. The two‐tailed Student's t tests were used. **P < 0.01. I, Huh7 cells expressing either OGT shRNA2 alone or with wild‐type eIF4E lentivirus were incubated with PE‐labelled anti‐AC133 antibody. The percentages of CD133+ cells in graphs were analysed by flow cytometry. Black line, control IgG staining; red line, CD133 staining. J, Cell lysates were examined by western blotting with indicated antibodies. The right panel showcases relative protein amounts of different groups. Error bars represent ±SD of triplicate experiments. **P < 0.01; n.s, no significance. K, Huh7 cells were collected and subjected to immunoprecipitation with antibody against eIF4E or normal mouse IgG. Total RNAs were purified from immunocomplexes and subjected to RT‐PCR to measure Sox2, OCT4, and KLF4 mRNAs associated with eIF4E Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30677218), licensed under a CC-BY license. Not internally tested by R&D Systems.

TP63 and SOX2 staining in cervical biopsies.Immunohistochemical staining of cervical biopsies. Bars = 50µm.(A) normal cervix no primary control; (B) normal cervix stained with anti-SOX2; (C) Squamous cell cervical carcinoma no primary control; (D) representative TP63, and (E) representative SOX2, staining of tumour cells. For both TP63 and SOX2 staining was seen in the nucleus of positive cells (examples indicated by solid arrows); negative cells were a minority of tumour cells (examples indicated by unfilled arrows). (F) Image analysis results of % nuclear +ve tumour cells in biopsies. Parallel sections from 11 cases were stained with SOX2 and TP63. Tumour cells were evaluated for their nuclear expression of the transcription factors. There was no significant difference between the data for SOX2 and TP63 (Wilcoxon signed rank test). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25531390), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Direct effects of glucocorticoids in ependymal glia. (A–I) Zebrafish spinal cord transverse sections. (A–C) Immunostaining of wild-types for Nr3c1 (green) and Gfap (red), plus DAPI staining (blue) with Dex treatment at 24 h post sham injury (A), 24 h (B) and 48 h post SCI (C). Nuclear Nr3c1 (active) localization in Gfap-positive cells around the central canal is stimulated with Dex treatment. (D–F) Immunostaining for EGFP (pan-cytoplasmic) in the SR4G transgenic reporter line (green) and filamentous Gfap (red) at 24 h post sham injury (D) and SCI with no treatment (E) or 24 h post SCI +Dex (F). Nr3c1 signaling is constitutively active in ependymal glia in uninjured controls (D), diminished after SCI (E), and was maintained after SCI + Dex. (G–I) Immunostaining for Sox2 (green), PCNA (red) and Gfap (blue) from 48 h post sham injury (G), 48 h post SCI followed by no treatment (H), or 48 h post SCI +Dex (I). Sox2 immunoreactivity is reduced in ependymal glia and neural precursor cells after Dex treatment. Representative images from 10 spinal cords per time point in each condition. Scale bars, 20 μm (A is the same as B,C; D is the same as E,F; G is the same as H,I). ∗denotes central canal. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31069223), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Epigenetic perturbation of enhancer clustering and genome-widebinding.(A) The fluctuation range (x) and amplitude (y) were obtainedby fitting the pair-correlation function of the indicated dataset with thefluctuation model. Figure 2 and Figure 2—figure supplement 1,Equations10–13. Supplementary file 1. Data from the same conditionwere grouped in separate ellipses. (B) Sox2 ChIP-exo peakdensity distribution in the wild-type and TSA treated (red dotted) cellsacross chromosome 1, 2, 3. In the upper panels, each chromosome was dividedto 500 bins. The color map correlates with the number of peaks in each bin.Top 7000 binding sites were considered in each condition. (C)Cumulative density histogram of the distances to transcription start sites(TSS's) of Sox2 ChIP-exo peaks in WT, Sox2 ChIP-exo peaks in the TSA treatedcells (red dotted), and random genomic positions (gray).DOI://dx.doi.org/10.7554/eLife.04236.013 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25537195), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Generation of KCNA5 knockout model. (A) Illustration of polycistronic self-replicating RNA used for reprogramming (Yoshioka et al., 2013). Reading frames for OCT4, KLF4, SOX2, GLIS1, and puromycin resistance are separated by 2A peptide-encoding or IRES sequences. (B) Phase contrast morphology (5 x) and alkaline phosphatase staining of the F1 wild-type hiPSC line generated using the RNA vector shown in (A). (C) Representative F1 karyogram indicating a normal male genotype. (D) The RNA vector is undetectable in established F1 hiPSCs following puromycin withdrawal, whereas freshly transfected parental fibroblasts display robust transgene expression (RT-qPCR data, n = 2). (E) Bisulfite sequencing of OCT4 and NANOG promoter regions of the indicated samples showing an hESC-like hypomethylated state in F1 cells. (F) RT-qPCR analysis of F1 hiPSCs in comparison to the original fibroblasts and a hESC control (n = 2). (G) Immunostains showing robust pluripotency factor expression in F1 hiPSCs at the protein level. (H) Illustration of the Kv1.5 channel. The voltage sensor in transmembrane helix S4 is indicated by symbols. The ion pore region is in-between segments S5 and S6. (I)KCNA5 knockout strategy based on simultaneously transfecting 4 CRISPR/Cas9 nickase vectors targeting the 5' end of the 1-exon gene body. A mutant clone generated this way displayed homozygous deletions causing a frame shift and premature stop as illustrated at the bottom. (J) PCR spanning the mutation site validates the sequencing-verified frame shift-causing deletion in KCNA5fs/fs cells at the DNA and RNA levels. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2017.00469/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Construction and expression of srRNA.A: Scheme of Self-Replicating srRNA constructs. B: Expression of reprogramming factors in OKS-iM 4F-srRNA, OKS-iG 4F-srRNA, OKS-iGM 5F-srRNA, and OKS-iGML 6F-srRNA on day 2 (left) and day 10 (right). BJ cells were co-transfected with srRNAs plus B18R mRNA (1:1 srRNA:B18R mRNA), selected with puromycin and cultured in Advanced DMEM/10% FBS for 10 days. C: qRT-PCR analysis of LIN28A and NANOG from 4F-srRNA and 5F-srRNA transfected BJ cells normalized to GAPDH. Cells were selected with puromycin after the transfection, and cultured in Advanced DMEM/10% FBS (Ad) or ES medium. Cells were collected on day 2, 4, 6, 8 and 10 for 5F-srRNA, and day 10 for 4F-srRNA (OKS-iM and OKS-iG). D: Immunoblot analysis of LIN28A and NANOG in 5F-srRNA transfected BJ cells on day 6 and 10. iPSC: iPSC clone generated with 5F-srRNA from BJ cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28750082), licensed under a CC-BY license. Not internally tested by R&D Systems.

The Majority of Ferret and Human, but Not Mouse, Sox2-Positive Tbr2-Negative BPs Exhibit Nuclear YAP. (G and H) that are shown at higher magnification (F′, F″, G′, G″, H′, and H″), as indicated; selected Tbr2-positive nuclei that are YAP negative in mouse, ferret, and human are outlined by white lines.(I–K) Quantification of the percentage of Tbr2-negative nuclei in the VZ (I), Tbr2-positive nuclei in the VZ (J), and Tbr2-positive nuclei in the SVZ (K) that are YAP positive in mouse E14.5, ferret E36, and human 11 wpc neocortex. Two or three images per embryo-fetus were taken, 30 randomly picked Tbr2-negative nuclei in the VZ (I) and Tbr2-positive nuclei in the VZ (J) and SVZ (K) were scored per image, and the values obtained were averaged for each embryo-fetus. Data are the mean of three or four embryos-fetuses.(A–C and F–H) Images are 1-μm optical sections. Scale bars represent 50 μm (A–C and F–H), 10 μm (A′, B′, and C′), and 20 μm (F′, F″, G′, G″, H′, and H″).(D, E, and I–K) Error bars indicate SEM; ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA, post hoc Tukey HSD).See also Figures S1 and S2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018127), licensed under a CC-BY license. Not internally tested by R&D Systems.

The Majority of Ferret and Human, but Not Mouse, Sox2-Positive Tbr2-Negative BPs Exhibit Nuclear YAP(A–C) Double immunofluorescence for YAP (green) and Sox2 (magenta), combined with DAPI staining (white), of mouse E14.5 (A), ferret E36 (B), and human 14 wpc (C) neocortex. Boxes indicate areas in the SVZ (A) and oSVZ (B and C) that are shown at higher magnification (A′, B′, and C′); selected Sox2-positive nuclei that are YAP negative in mouse and YAP positive in ferret and human are outlined by white lines; arrowheads indicate a YAP-positive basal process of a bRG. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018127), licensed under a CC-BY license. Not internally tested by R&D Systems.

The Majority of Ferret and Human, but Not Mouse, Sox2-Positive Tbr2-Negative BPs Exhibit Nuclear YAP .(F–H) Double immunofluorescence for YAP (green) and Tbr2 (magenta), combined with DAPI staining (white), of mouse E14.5 (F), ferret E36 (G), and human 11 wpc (H) neocortex. Boxes indicate areas in the VZ and SVZ (F) or iSVZ (G and H) that are shown at higher magnification (F′, F″, G′, G″, H′, and H″), as indicated; selected Tbr2-positive nuclei that are YAP negative in mouse, ferret, and human are outlined by white lines.(I–K) Quantification of the percentage of Tbr2-negative nuclei in the VZ (I), Tbr2-positive nuclei in the VZ (J), and Tbr2-positive nuclei in the SVZ (K) that are YAP positive in mouse E14.5, ferret E36, and human 11 wpc neocortex. Two or three images per embryo-fetus were taken, 30 randomly picked Tbr2-negative nuclei in the VZ (I) and Tbr2-positive nuclei in the VZ (J) and SVZ (K) were scored per image, and the values obtained were averaged for each embryo-fetus. Data are the mean of three or four embryos-fetuses.(A–C and F–H) Images are 1-μm optical sections. Scale bars represent 50 μm (A–C and F–H), 10 μm (A′, B′, and C′), and 20 μm (F′, F″, G′, G″, H′, and H″).(D, E, and I–K) Error bars indicate SEM; ∗∗p < 0.01, ∗∗∗p < 0.001 (one-way ANOVA, post hoc Tukey HSD).See also Figures S1 and S2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31018127), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemical analysis of Trp53-/-Notch1-/-Notch2-/- and Trp53-/-Rbpj-/- tumors. (A) A Trp53-/-Notch1-/-Notch2-/- tumor in the SVZ. Streams of tumor cells invading the brain parenchyma are indicated by arrowheads. The GFP expression from the Rosa-CAG::GFP Cre-reporter indicates derivation from Hes5+ cells. (B) Expression of GFP, progenitor (SOX2) and glial markers (OLIG2, GFAP), as well as staining for mitotically active cells (PCNA) and immature neurons (DCX) in Trp53-/-Notch1-/-Notch2-/- and Trp53-/-Rbpj-/- tumors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36358826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Deregulation of SOX2, ASCL1&OLIG2 TFs in p27KO cultures at the onset of differentiation. e Immunocytochemistry for p27 (red) in combination with ASCL1, OLIG2 or SOX2 (green) in 2 + 1DIV NPCs. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36627412), licensed under a CC-BY license. Not internally tested by R&D Systems.

Padi2/3 DKO TSCs exhibit diminished stem cell potential&are prone to differentiate into large trophoblast giant cells (TGCs). (D) Immunofluorescence staining on WT&Padi2/3 DKO TSCs for trophoblast stem cell markers CDX2&SOX2. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36010543), licensed under a CC-BY license. Not internally tested by R&D Systems.

Unedited images from samples featured in Figure 6.Stage 39–40 larvae were amputated and processed for whole-mount immunostaining at the indicated hour post amputation (hpa), with the exception of the 0 h group, which was first fixed and then amputated to represent the pre-amputation group. Images show representative total z-projections of whole-mount immunostained samples for each group corresponding to the edited images shown in Figure 6. Transverse red dashed line indicates amputation plane. Scale bar, 100 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/33955353), licensed under a CC-BY license. Not internally tested by R&D Systems.

Notch1 and Trp53 deletion in Hes5+ cells leads to formation of forebrain tumors in adult mice. (A) Kaplan–Meier curves showing survival of Hes5::CreERT2 Trp53-/-Notch1-/- and Trp53-/-Notch2-/- mutant mice. (B) A Trp53-/-Notch1-/- tumor in the forebrain. The GFP expression from the Rosa-CAG::GFP Cre-reporter indicates derivation from Hes5+ cells. (C) Percentages of Trp53-/-Notch1-/- and Trp53-/-Notch2-/- mice that developed hyperplasia or tumors in the brain. (D) A Trp53-/-Notch1-/- tumor (dashed line) in the anterior forebrain. In contrast, the forebrain of Trp53-/-Notch2-/- mice appears grossly normal. (E) Expression of GFP, progenitor (SOX2) and glial markers (OLIG2, GFAP), as well as staining for mitotically active cells (PCNA) and immature neurons (DCX) in Trp53-/-Notch1-/- tumors. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36358826), licensed under a CC-BY license. Not internally tested by R&D Systems.

Wnt signaling downregulates Tcf3 expression in mouse ESCs.A. qRT-PCR analysis of Tcf3 in wild type, ApcNN and ApcMin/Min ESCs. Actb was used as an internal control; bars represent n = 2 ± SD. B. Western blot analysis of the core pluripotency markers Oct4, Nanog, Sox2 and Tcf3 on protein lysates isolated from two independent ApcNN clones and wild type control ESCs. Actb and Tubulin were used as an internal control. C–D. qRT-PCR analysis of Tcf3 in wild type ESCs treated for different time intervals with Wnt3a conditioned medium (C), or with the GSK-inhibitor SB-216763 (D). L-medium and DMSO were employed as controls, respectively. Actb was used as an internal control; bars represent n = 2 ± SD. E. Time course western blot analysis of Tcf3 expression in wild type ESCs treated with Wnt3a conditioned medium (Wnt3a CM) or control L-medium (LM). Actb was used as an internal control. F. qRT-PCR analysis of Tcf3 and Wnt target genes Axin2 and Cdx1 in wild type ESC treated for 48 h with different concentrations of GSK-inhibitor SB-216763 or DMSO as control. Actb was used as an internal control; bars represent n = 2 ± SD. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23658527), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fetal rat lung stained for Sox2 (red), co-stained with DAPI (blue). Image from a verified customer review.

SOX2, OCT2, and PRRX1 are downstream effectors of the SETD2‐regulated EMT program. (A) Expression of SOX2, OCT2, and PRRX1 in TGF‐ beta ‐treated WT (72 h), SETD2 KO, and SETD2 rescue tested by RT‐qPCR (run in triplicate). (B) Migration capacity by wound healing assay, (C) invasiveness by transwell assay, and (D) stemness by 3D spheroid formation assay in RPTEC WT GFP (control vector), SETD2 KO1 and KO2, and SOX2/OCT2/PRRX1‐transduced WT RPTEC lines. Images are taken at 4× magnification, scale bar: 1000 μm for (B) and (D) and at 2.5× magnification, scale bar: 1200 μm for (C). Data are represented as mean ± SEM for triplicate reactions for B–D. P‐value is calculated by one‐way ANOVA in (A), (B), and (D). Two‐way ANOVA is used for statistical test for (C). ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; ns, P ≥ 0.05. (E) Model of the SETD2 loss‐driven EMT program through cell intrinsic (transcriptional) and cell extrinsic (paracrine) mechanisms. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37418588), licensed under a CC-BY license. Not internally tested by R&D Systems.

Temporally coordinated expression of Sox2, Tbr2, NueroD, and Prox1 in gfap-GFP+ GNPs and differentiating granule neurons Gfap-GFP+/Sox2+ progenitor cells were found at the dentate neuroepithelium at E14 (arrow in A1). Sox2 expression was found at the VZ. Tbr2+ GNPs are then observed (red, arrow in A2). Tbr2 expression is followed by NeuroD expression (A3). Tbr2+/NeuroD+ GNPs are found (arrow, A3). The Tbr2+ GNPs (red) migrate toward the DG primordium and co-express Prox1 (green), a marker for early differentiating dentate granule neurons (arrow in A4). Gfap-GFP+ progenitors sequentially express Sox2, Tbr2, NeuroD1, and then, Prox1 in the developing DG at E16 (arrows in B1–B4, respectively). Some Sox2+/Tbr2+, Tbr2+/NeuroD+, and Tbr2+/Prox1+ GNPs are found. Similarly, gfap-GFP+ progenitor cells contribute to Tbr2+/NeuroD+ (arrows in D1,D2), Prox1+ GNPs (arrows in D3,D4) at P3. Schematic drawing of the expression patterns of gfap-GFP and transcription factors during the differentiation of GNPs (E). Scale bars; 200 μm in (A1–A4, B1–B4, C1–C3, D1,D3); 50 μm in (D2,D4). Image collected and cropped by CiteAb from the following open publication (//www.frontiersin.org/articles/10.3389/fnins.2024.1425849/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2018U-100UG
Alexa Fluor 405	AF2018V-100UG
Alexa Fluor 488	AF2018G-100UG
Alexa Fluor 532	AF2018X-100UG
Alexa Fluor 594	AF2018T-100UG
Alexa Fluor 647	AF2018R-100UG
Alexa Fluor 700	AF2018N-100UG
Alexa Fluor 750	AF2018S-100UG
Alexa Fluor Plus 405	AF2018AFP405-100UG
Alexa Fluor Plus 488	AF2018AFP488-100UG
Alexa Fluor Plus 555	AF2018AFP555-100UG
Alexa Fluor Plus 594	AF2018AFP594-100UG
Alexa Fluor Plus 647	AF2018AFP647-100UG
Alexa Fluor Plus 680	AF2018AFP680-100UG
Allophycocyanin	AF2018A
Biotin	BAF2018
CoraFluor 1	AF2018CL1
DyLight 350	AF2018D
DyLight 405	AF2018E
DyLight 488	AF2018K
DyLight 550	AF2018L
DyLight 594	AF2018M
DyLight 650	AF2018W
DyLight 680	AF2018Y
DyLight 755	AF2018Z
FITC	AF2018F
HRP	AF2018H
Janelia Fluor 525	AF2018JF525
Janelia Fluor 549	AF2018I
Janelia Fluor 646	AF2018J
Janelia Fluor 669	AF2018JF669
NL493	NL20181G
PE	AF2018P
PerCP	AF2018C
mFluor Violet 450 SE	AF2018MFV450
mFluor Violet 500 SE	AF2018MFV500
mFluor Violet 610 SE	AF2018MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	NL20181G
Carrier Free	AF2018-SP

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MAB2018

SOX2 Antibody (245610) [Unconjugated]

(5 Reviews)

(290 Publications)

100 μg

MAB2018

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder." class="big_lightbox">

SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of human Glioblastoma using Mouse Anti-Human SOX2, Monoclonal Antibody (Catalog # MAB2018) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder." alt="SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of human Glioblastoma using Mouse Anti-Human SOX2, Monoclonal Antibody (Catalog # MAB2018) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus. Protocol available in COMET™ Panel Builder." class="big_thumb" />

AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

E-Cadherin and SOX2 were detected in BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human E-Cadherin Antigen Affinity-purified Polyclonal Antibody (<a class=

AF648) and 10 µg/mL Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for E-Cadherin using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and for SOX2 using the NorthernLights 493-conjugated Anti-Mouse Secondary Antibody (green; NL009). Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, D3 mouse embryonic stem cell line, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=

HAF018). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX2 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/ Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018, filled histogram) or isotype control antibody (<a class=

MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 50 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

SOX2 was detected in immersion fixed paraffin-embedded sections of glioblastoma using Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Western blotting analysis of the different antigens studied in cell lines (NS and AC) and in primary tumors. (a) Stemness antigens and differentiation antigens prevail in NS and AC, respectively. (b) Densitometric quantification of bands in NS and in the corresponding primary tumors. (c) Densitometric quantification of bands in AC and in the corresponding primary tumors. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21869887), licensed under a CC-BY license. Not internally tested by R&D Systems.

Double immunofluorescence staining of core and periphery in orthotopic model with five different patient derived GBM spheroid cultures.Tissues were stained with Dapi (blue), Vimentin (green) and Sox-2 (red), Bmi-1 (red) and Glut-3 (red). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both central part and periphery for Sox-2 (G), Bmi-1 (H) and Glut-3 (I). Statistical comparison was performed using student’s t-test. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Soluble factors increase the numbers of Hes3+ cells in the adult rat hippocampus.(a) Treatment with the soluble factors induced a powerful increase in the number of Sox2+ cells in the hilus [Quantifications are from images taken from the tip of the hilus to approximately 1,300 micrometers towards the CA3 region – representative images are shown. Staining for the young neuron marker doublecortin (DCX) helps to visualize the subgranular zone]. (b) The same treatment also induced an increase in the numbers of Hes3+ cells in the hilus. (c) Sox2+ cells inside the hilus co-expressed Hes3; in contrast, Sox2+ cells in the subgranular zone did not co-express Hes3. (d,e) Quantification graphs of the increases in Sox2+ and Hes3+ cell numbers in the hilus (p<0.05). For the graph, the number of cells in the sham operated control animals was set to 100%. [Width of images: a, 650 micrometers; b, 650 micrometers]. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0051630), licensed under a CC-BY license. Not internally tested by R&D Systems.

Double immunofluorescence staining of core and periphery of patient glioblastomas.Histological sections were stained with Dapi (blue), IDH1 (green) and Sox-2 (red) (AA-BE), Bmi-1 (red) (CA-DE) and Glut-3 (red) (EA to FE). The software-based classifier is shown in the right column. The classifier illustrates tumor cells co-expressing markers of interest in yellow and tumor cells not co-expressing markers of interest in green. The fluorescence stainings were quantified in both core and periphery for Sox-2 (G,J), Bmi-1 (H,K) and Glut-3 (I,L). Statistical comparison was performed using student’s t-test and ANOVA, ** p< 0.01. Scalebar: 200μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0155106), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>CEP-1347 induces the differentiation of cancer stem cells into non-cancer stem cells(A) Glioma stem cells (GS-Y01, GS-Y03, and GS-NCC01), pancreatic cancer stem cells (PANC-1 CSLC), and ovarian cancer stem cells (A2780 CSLC, and TOV21G CSLC) treated without (Control) or with CEP-1347 (300 nM for PANC-1 CSLC, 200 nM for the others) for 6 days were subjected to flow cytometric analysis of the cell-surface expression of CD133. Representative flow cytometric plots together with the percentages of CD133-positive cells are shown. (B) Cells treated as described in (A) were subjected to immunoblot analysis of the indicated proteins. (C) PANC-1 CSLC cells treated without (Control: lane 1) or with CEP-1347 (lane 2) for 6 days and those cultured in the presence of CEP-1347 for 6 days and then in its absence for the indicated days (lanes 3-5) were subjected to immunoblot analysis of the indicated proteins. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22033), licensed under a CC-BY license. Not internally tested by R&D Systems.

Fluoxetine inhibits the differentiation of hypothalamic neuroprogenitor cells.Hypothalamic neuroprogenitor cells were differentiated for 18 days, in the presence or absence of fluoxetine (1 µM). Representative confocal photomicrographs for mature neurons marker Neu-N (green) (A). Fluoxetine decreases the percentage of Neu-N positive cells and the incubation with Trk receptors inhibitor K252a (24 h) partially reverses this effect (B). Representative confocal photomicrographs for the progenitor cells marker SOX-2 (green) (C). Fluoxetine increases the percentage of SOX-2 positive cells and the incubation with K252a (24 h) does not change this effect (D). Fluoxetine does not up regulate the levels of neurotrophic factor BDNF but incubation with K252a (24 h) results in a compensatory increase of BDNF mRNA levels (E). DAPI (blue), nuclear staining. Mean ± SEM; n = 4/5; One-Way ANOVA; ns, p>0.05; *, p<0.05; ***, p<0.001 compared to control; NS, p>0.05, #, p<0.05 compared to fluoxetine. Scale bar: 50 µm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24598761), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hes3 is a marker of normal and cancer human stem cells.(A–C) Hes3+ cell in the striatum of non-cancerous adult human brain tissue (blood vessels identified by RECA-1 expression), human hemangioblastoma (HBM) biopsy (HBM Hes3+ megakaryocytes shown), human glioblastoma multiforme (GBM) biopsy (Hes3 co-expressed with prominin). (D) Fetal cortical cells sorted for prominin express Sox2 and Hes3. (E) Enrichment for Sox2+ and Hes3+ cells by magnetic sorting using an anti-prominin antibody. (Size bar, 50 µm). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20195471), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry of a parallel section of Figure 1. (a) Poor GFAP expression. (b) High Nestin expression. (c) SOX2 is highly expressed in nuclei of the external slope of circumscribed necrosis. (d) REST expression, all DAB. (e) CD133-positive area, frozen section. (f) Musashi-1-positive area. Scale bar 50 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21869887), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neural induction of hPSCs. (a) Both hPSC culture and neural differentiation were performed on LN521 substrate. Cortical neurons were induced with dual SMAD inhibition, expanded in the presence of FGF2, and matured with support by a selection of neurotrophic factors. Neural progenitor cells (NPCs) could be cryopreserved at day 21 and plated at day 32 for final experiments, including microelectrode array (MEA) measurements. (b) Three different hPSC lines (one hESC line, 08/023 and two hiPSC lines, 10212.EURCCs and IMR90-4) were characterized for their efficiency in producing neuroectodermal cells in response to 12-day neural induction by dual SMAD inhibition. Cells were stained for pluripotency marker Oct4 at the pluripotent stage and after 12 days of neural induction. The presence of early neuroectodermal markers was evaluated with Sox2, FoxG1 and Pax6 staining. (c) After 21 days of differentiation, the culture contained vimentin- and Pax6-positive NPCs that could be cryopreserved. Additionally, the first Tbr2- and MAP2-positive neurons were detected at this time point. (d) The percentages of Pax6-positive cells were quantified at four time points of differentiation (mean ± s.e.m., n = 5–14, data derived from 1–3 independent differentiations). Statistical analysis was performed with the Mann-Whitney U test to compare differences between day 12 and day 46 within each hPSC line, and significant p-values are presented in the image. The scale bar is 50 µm in all images. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31748598), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of TRNDi009-C iPSC line. A) Left: phase contrast imaging of TRNDi009-C colonies grown on Geltrex at passage 6. Right: Representative immunofluorescent images of iPSCs positive for stem cell markers: SOX2, OCT4, NANOG, and SSEA4. Nucleus is labelled with Hoechst 33342 (blue). B) Cytogenetic analysis showing a normal karyotype (46, XX). C) Detection of heterozygous mutation of a p.L302P (c.905 T > C) in exon 2 of the SMPD1 gene. D) Flow cytometry analysis of pluripotency protein markers: NANOG and TRA-1–60. E) RT-PCR verification for the clearance of the Sendai virus from reprogrammed cells. Sendai virus vector transduced fibroblasts were used as a positive control. F) Pathological analysis of teratoma from TRNDi009-C iPSC, showing a normal ectodermal, mesodermal, and endodermal differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31132580), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of TRNDi007-B iPSC line.A) Left: Phase contrast imaging of TRNDi007-B colonies. Right: Representative immunofluorescent images of iPSCs showing expression of stem cell markers: SOX2, OCT4, SSEA-4, and NANOG. Nucleus is stained with Hoechst dye (in blue). B) Flow cytometry analysis of pluripotency protein markers: TRA-1-60, NANOG and SSEA-4. C) Cytogenetic analysis showing a normal karyotype (46, XY). D) Detection of homozygous mutation of c.2560 C > T in exon 18 of the GAA gene. E) RT-PCR verification of the clearance of Sendai virus from the reprogrammed cells. Sendai virus vector transduced fibroblasts was used as positive control. F) Histological analysis of teratomas produced by TRNDi007-B iPSCs. Representative images showing the presence of ectodermal, endodermal and mesodermal derivatives. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31026687), licensed under a CC-BY license. Not internally tested by R&D Systems.

CEP-1347 induces the differentiation of cancer stem cells into non-cancer stem cells(A) Glioma stem cells (GS-Y01, GS-Y03, and GS-NCC01), pancreatic cancer stem cells (PANC-1 CSLC), and ovarian cancer stem cells (A2780 CSLC, and TOV21G CSLC) treated without (Control) or with CEP-1347 (300 nM for PANC-1 CSLC, 200 nM for the others) for 6 days were subjected to flow cytometric analysis of the cell-surface expression of CD133. Representative flow cytometric plots together with the percentages of CD133-positive cells are shown. (B) Cells treated as described in (A) were subjected to immunoblot analysis of the indicated proteins. (C) PANC-1 CSLC cells treated without (Control: lane 1) or with CEP-1347 (lane 2) for 6 days and those cultured in the presence of CEP-1347 for 6 days and then in its absence for the indicated days (lanes 3-5) were subjected to immunoblot analysis of the indicated proteins. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29212273), licensed under a CC-BY license. Not internally tested by R&D Systems.

(A) Differentiation markers for the endoderm (FOXA2, PDX1), mesoderm ( alpha -actin, brachyury), and ectoderm ( beta 3 Tubulin, Otx2) were detected for the MUCG01, MUCG02, and MUCG03 clinical-grade hESC lines by immunocytochemistry. (B) Standard karyotypes without chromosomal aberration were detected for all hESC lines MUCG01: 46,XX; MUCG02: 46,XX; and MUCG03: 46,XY. (C) Pluripotency markers Oct3/4, Sox2, and Nanog were detected for the MUCG01, MUCG02, and MUCG03 clinical-grade hESC lines by immunocytochemistry. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36293356), licensed under a CC-BY license. Not internally tested by R&D Systems.

human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue). " class="big_lightbox">

Adult stem cells isolated from human lung biopsy tissue were cultured following the steps detailed in the <a class=

human lung organoid culture protocol. Lung organoids were stained with a (A) Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) and a rabbit anti-human cytokeratin 5 (KRT5) monoclonal antibody (green) to visualize basal cells; a (B) Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (Catalog # AF1916; red) to visualize basal cells; and a (C, D) Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (Catalog # MAB2018; red). All samples were counterstained with DAPI (Catalog # 5748; blue). " class="big_thumb" />

Characterization of NS/PCs derived from hiPSCs. b, Representative images (b) of immunocytochemical analysis of hiPSC-NS/PCs (NS/PC-A & NS/PC-B) using antibodies against SOX1, SOX2, & NESTIN. Inset: Hoechst nuclear staining of the same field. Scale bar, 50 μm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37286713), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of cell lines.(A) H&E staining of tumor 5653, the arrow indicates the location of the tumor. The right panel shows a higher magnification. (B) Genotyping PCR on genomic wildtype mouse, genomic NPcis mouse (heterozygous for Nf1 and Tp53) DNA and DNA extracted from cell lines 17, 5653 and 5746. The glioma cell lines show complete loss of the wildtype allele for Nf1 and Tp53. (C) Western blot shows the expression of markers consistent with OPC lineage origin of our cell lines. SOX2 is expressed in Neural Progenitor cells (NPC) and Oligodendrocyte precursor cells (OPC); NG2 is expressed in OPCs. Markers (CPNase and MAG) expressed in pre-oligodendrocytes (pre-OL) or mature oligodendrocytes (OL) are not present, highlighting the progenitor status of our lines [22]. (D) The ATRX mutation (E2281*) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with STOP mutation. (E) The homozygous 4bp NF1 deletion (p.LF1247fs*18) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with the homozygous 4bp deletion inducing a frameshift. (F) Western blot for TP53 and CDKN2A, showing that both genes are absent in TM31, but not in glioma line LN319. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36730269), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , , ,

Applications: , , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2018U-100UG
Alexa Fluor 405	IC2018V-100UG
Alexa Fluor 488	IC2018G
Alexa Fluor 532	FAB2018X
Alexa Fluor 594	IC2018T-100UG
Alexa Fluor 647	IC2018R-100UG
Alexa Fluor 700	IC2018N-100UG
Alexa Fluor 750	IC2018S-100UG
Alexa Fluor Plus 405	FAB2018AFP405-100UG
Alexa Fluor Plus 488	FAB2018AFP488-100UG
Alexa Fluor Plus 555	FAB2018AFP555-100UG
Alexa Fluor Plus 594	FAB2018AFP594-100UG
Alexa Fluor Plus 647	FAB2018AFP647-100UG
Alexa Fluor Plus 680	FAB2018AFP680-100UG
Allophycocyanin	IC2018A
Biotin	FAB2018B
CoraFluor 1	FAB2018CL1
DyLight 350	FAB2018D
DyLight 405	FAB2018E
DyLight 488	FAB2018K
DyLight 550	FAB2018L
DyLight 594	FAB2018M
DyLight 650	FAB2018W
DyLight 680	FAB2018Y
DyLight 755	FAB2018Z
FITC	FAB2018F
HRP	FAB2018H
Janelia Fluor 525	FAB2018JF525
Janelia Fluor 549	FAB2018I
Janelia Fluor 646	FAB2018J
Janelia Fluor 669	FAB2018JF669
Phycoerythrin	IC2018P-025
Phycoerythrin	IC2018P-100
mFluor Violet 450 SE	FAB2018MFV450
mFluor Violet 500 SE	FAB2018MFV500
mFluor Violet 610 SE	FAB2018MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC2018A
Carrier Free	IC2018G
Carrier Free	MAB2018-SP
Carrier Free	IC2018P-100
Carrier Free	IC2018P-025
Carrier Free	IC2018V-100UG
Carrier Free	IC2018U-100UG
Carrier Free	IC2018T-100UG
Carrier Free	IC2018S-100UG
Carrier Free	IC2018R-100UG
Carrier Free	IC2018N-100UG

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NB110-37235

SOX2 Antibody - BSA Free

(4 Reviews)

(38 Publications)

0.1 ml

NB110-37235

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Brain Cortex using Rabbit Anti-Mouse SOX2, Monoclonal Antibody (Catalog # NB110-37235) at 1:125 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder." alt="SOX2 Antibody was detected in immersion fixed paraffin-embedded sections of mouse Brain Cortex using Rabbit Anti-Mouse SOX2, Monoclonal Antibody (Catalog # NB110-37235) at 1:125 dilution at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the nucleus and cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

Immunohistochemistry-Paraffin: SOX2 Antibody [NB110-37235] - Staining of human uterus, endometrial glands.

Western Blot: SOX2 Antibody [NB110-37235] - Detection of SOX2 in mouse brain lysate using NB110-37235 at 0.5 ug/mL.

Flow Cytometry: SOX2 Antibody [NB110-37235] - An intracellular stain was performed on A549 with NB110-37235 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylgiht 550.

<b>Biological Strategies Validation. </b>Western Blot: SOX2 Antibody [NB110-37235] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - Rat FR cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow Cytometry: SOX2 Antibody - BSA Free [NB110-37235] - An intracellular stain was performed on U-251 MG cells with SOX2 Antibody NB110-37235 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: SOX2 Antibody [NB110-37235] - Expression of pluripotency factor Sox2 in human head and neck cancer cell lines FaDu and HN5. WB image submitted by a verified customer review.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - Staining of SOX2 in NTERA2 cells with Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained with Dylight 550 (Red) and DAPI (Blue), respectively.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody [NB110-37235] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-SOX2 (NB110-37235) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunocytochemistry/Immunofluorescence: SOX2 Antibody - BSA Free [NB110-37235] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with SOX2 Antibody (NB110-37235) at 2ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Reactivity:

Hu, Mu, Rt, Ca, Sh, Ch

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Sh, Ch

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB110-37235AF350
Alexa Fluor 405	NB110-37235AF405
Alexa Fluor 488	NB110-37235AF488
Alexa Fluor 532	NB110-37235AF532
Alexa Fluor 594	NB110-37235AF594
Alexa Fluor 647	NB110-37235AF647
Alexa Fluor 700	NB110-37235AF700
Alexa Fluor 750	NB110-37235AF750
Alexa Fluor Plus 405	NB110-37235AFP405
Alexa Fluor Plus 488	NB110-37235AFP488
Alexa Fluor Plus 555	NB110-37235AFP555
Alexa Fluor Plus 594	NB110-37235AFP594
Alexa Fluor Plus 647	NB110-37235AFP647
Alexa Fluor Plus 680	NB110-37235AFP680
Allophycocyanin	NB110-37235APC
Biotin	NB110-37235B
CoraFluor 1	NB110-37235CL1
DyLight 350	NB110-37235UV
DyLight 405	NB110-37235V
DyLight 488	NB110-37235G
DyLight 550	NB110-37235R
DyLight 594	NB110-37235DL594
DyLight 650	NB110-37235C
DyLight 680	NB110-37235FR
DyLight 755	NB110-37235IR
FITC	NB110-37235F
HRP	NB110-37235H
Janelia Fluor 525	NB110-37235JF525
Janelia Fluor 549	NB110-37235JF549
Janelia Fluor 646	NB110-37235JF646
Janelia Fluor 669	NB110-37235JF669
PE	NB110-37235PE
PerCP	NB110-37235PCP
mFluor Violet 450 SE	NB110-37235MFV450
mFluor Violet 500 SE	NB110-37235MFV500
mFluor Violet 610 SE	NB110-37235MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB110-37235SS

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NBP2-29623

SOX2 Antibody (4G8) - BSA Free

(1 Publication)

0.1 mg

NBP2-29623

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].

Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].

Reactivity:

Hu, Mu, Ch, Pm, Sh

Species Glossary

Reactivity: Hu, Mu, Ch, Pm, Sh

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-29623AF350
Alexa Fluor 405	NBP2-29623AF405
Alexa Fluor 488	NBP2-29623AF488
Alexa Fluor 532	NBP2-29623AF532
Alexa Fluor 594	NBP2-29623AF594
Alexa Fluor 647	NBP2-29623AF647
Alexa Fluor 700	NBP2-29623AF700
Alexa Fluor 750	NBP2-29623AF750
Alexa Fluor Plus 405	NBP2-29623AFP405
Alexa Fluor Plus 488	NBP2-29623AFP488
Alexa Fluor Plus 555	NBP2-29623AFP555
Alexa Fluor Plus 594	NBP2-29623AFP594
Alexa Fluor Plus 647	NBP2-29623AFP647
Alexa Fluor Plus 680	NBP2-29623AFP680
Allophycocyanin	NBP2-29623APC
Biotin	NBP2-29623B
CoraFluor 1	NBP2-29623CL1
DyLight 350	NBP2-29623UV
DyLight 405	NBP2-29623V
DyLight 488	NBP2-29623G
DyLight 550	NBP2-29623R
DyLight 594	NBP2-29623DL594
DyLight 650	NBP2-29623C
DyLight 680	NBP2-29623FR
DyLight 755	NBP2-29623IR
FITC	NBP2-29623F
HRP	NBP2-29623H
Janelia Fluor 525	NBP2-29623JF525
Janelia Fluor 549	NBP2-29623JF549
Janelia Fluor 646	NBP2-29623JF646
Janelia Fluor 669	NBP2-29623JF669
PE	NBP2-29623PE
PerCP	NBP2-29623PCP
mFluor Violet 450 SE	NBP2-29623MFV450
mFluor Violet 500 SE	NBP2-29623MFV500
mFluor Violet 610 SE	NBP2-29623MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-29623SS
Azide and BSA Free	NBP2-80957

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NBP2-24390

SOX2 Antibody

(2 Publications)

0.1 mg

NBP2-24390

Reactivity:

Hu, Mu, Po, Ch, Fe, Pm, Rt, Bv, Pm, Ma-Op

Species Glossary

Reactivity: Hu, Mu, Po, Ch, Fe, Pm, Rt, Bv, Pm, Ma-Op

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6467A

Alt Catalog# IMG-6467A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-53407

SOX2 Antibody (SOX2/1791)

100 μg

NBP2-53407

Western Blot: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1791).

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1791).

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1791).

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Formalin-fixed, paraffin-embedded Mouse Brain stained with SOX2 Antibody (SOX2/1791).

Protein Array: SOX2 Antibody (SOX2/1791) [NBP2-53407] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1791) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54594AF350
Alexa Fluor 405	NBP2-54594AF405
Alexa Fluor 488	NBP2-54594AF488
Alexa Fluor 532	NBP2-54594AF532
Alexa Fluor 594	NBP2-54594AF594
Alexa Fluor 647	NBP2-54594AF647
Alexa Fluor 700	NBP2-54594AF700
Alexa Fluor 750	NBP2-54594AF750
Alexa Fluor Plus 405	NBP2-54594AFP405
Alexa Fluor Plus 488	NBP2-54594AFP488
Alexa Fluor Plus 555	NBP2-54594AFP555
Alexa Fluor Plus 594	NBP2-54594AFP594
Alexa Fluor Plus 647	NBP2-54594AFP647
Alexa Fluor Plus 680	NBP2-54594AFP680
Allophycocyanin	NBP2-54594APC
Biotin	NBP2-54594B
CoraFluor 1	NBP2-54594CL1
DyLight 350	NBP2-54594UV
DyLight 405	NBP2-54594V
DyLight 488	NBP2-54594G
DyLight 550	NBP2-54594R
DyLight 594	NBP2-54594DL594
DyLight 650	NBP2-54594C
DyLight 680	NBP2-54594FR
DyLight 755	NBP2-54594IR
FITC	NBP2-54594F
HRP	NBP2-54594H
Janelia Fluor 525	NBP2-54594JF525
Janelia Fluor 549	NBP2-54594JF549
Janelia Fluor 646	NBP2-54594JF646
Janelia Fluor 669	NBP2-54594JF669
PE	NBP2-54594PE
mFluor Violet 450 SE	NBP2-54594MFV450
mFluor Violet 500 SE	NBP2-54594MFV500
mFluor Violet 610 SE	NBP2-54594MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54594-100ug

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NBP2-53408

SOX2 Antibody (SOX2/1792)

100 μg

NBP2-53408

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with SOX2 Antibody (SOX2/1792).

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Formalin-fixed, paraffin-embedded Mouse Stomach stained with SOX2 Antibody (SOX2/1792).

Protein Array: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX2 Antibody (SOX2/1792) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Immunohistochemistry-Paraffin: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Analysis of SOX2 stained human head-neck cancer sample. Image from verified customer review.

Western Blot: SOX2 Antibody (SOX2/1792) [NBP2-53408] - Western Blot of Recombinant SOX2 protein fragment and NCCIT cell lysate using SOX2 Antibody (SOX2/1792).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-54593AF350
Alexa Fluor 405	NBP2-54593AF405
Alexa Fluor 488	NBP2-54593AF488
Alexa Fluor 532	NBP2-54593AF532
Alexa Fluor 594	NBP2-54593AF594
Alexa Fluor 647	NBP2-54593AF647
Alexa Fluor 700	NBP2-54593AF700
Alexa Fluor 750	NBP2-54593AF750
Alexa Fluor Plus 405	NBP2-54593AFP405
Alexa Fluor Plus 488	NBP2-54593AFP488
Alexa Fluor Plus 555	NBP2-54593AFP555
Alexa Fluor Plus 594	NBP2-54593AFP594
Alexa Fluor Plus 647	NBP2-54593AFP647
Alexa Fluor Plus 680	NBP2-54593AFP680
Allophycocyanin	NBP2-54593APC
Biotin	NBP2-54593B
CoraFluor 1	NBP2-54593CL1
DyLight 350	NBP2-54593UV
DyLight 405	NBP2-54593V
DyLight 488	NBP2-54593G
DyLight 550	NBP2-54593R
DyLight 594	NBP2-54593DL594
DyLight 650	NBP2-54593C
DyLight 680	NBP2-54593FR
DyLight 755	NBP2-54593IR
FITC	NBP2-54593F
HRP	NBP2-54593H
Janelia Fluor 525	NBP2-54593JF525
Janelia Fluor 549	NBP2-54593JF549
Janelia Fluor 646	NBP2-54593JF646
Janelia Fluor 669	NBP2-54593JF669
PE	NBP2-54593PE
mFluor Violet 450 SE	NBP2-54593MFV450
mFluor Violet 500 SE	NBP2-54593MFV500
mFluor Violet 610 SE	NBP2-54593MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-54593-100ug

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NBP3-32972

SOX2 Antibody (PO00-28)

100 ul

NBP3-32972

Immunohistochemistry: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunohistochemical analysis of paraffin-embedded human trachea tissue with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32972) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (PO00-28) [NBP3-32972] - Immunocytochemistry analysis of F9 cells labeling SOX2 with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SOX2 antibody (NBP3-32972) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Western blot analysis on different lysates with NBP3-32972 at 1/2,000 dilution. Lane 1: NCCIT cell lysate Lane 2: F9 cell lysate Lane 3: HeLa cell lysate (negative) Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32972 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on different lysates with NBP3-32972 at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 34 kDa Observed band size: 36 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32972 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP2-89196

SOX2 Antibody (017) - Azide and BSA Free

(7 Publications)

100 ul

NBP2-89196

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-89196AF350
Alexa Fluor 405	NBP2-89196AF405
Alexa Fluor 488	NBP2-89196AF488
Alexa Fluor 532	NBP2-89196AF532
Alexa Fluor 594	NBP2-89196AF594
Alexa Fluor 647	NBP2-89196AF647
Alexa Fluor 700	NBP2-89196AF700
Alexa Fluor 750	NBP2-89196AF750
Alexa Fluor Plus 405	NBP2-89196AFP405
Alexa Fluor Plus 488	NBP2-89196AFP488
Alexa Fluor Plus 555	NBP2-89196AFP555
Alexa Fluor Plus 594	NBP2-89196AFP594
Alexa Fluor Plus 647	NBP2-89196AFP647
Alexa Fluor Plus 680	NBP2-89196AFP680
Allophycocyanin	NBP2-89196APC
Biotin	NBP2-89196B
CoraFluor 1	NBP2-89196CL1
DyLight 350	NBP2-89196UV
DyLight 405	NBP2-89196V
DyLight 488	NBP2-89196G
DyLight 550	NBP2-89196R
DyLight 594	NBP2-89196DL594
DyLight 650	NBP2-89196C
DyLight 680	NBP2-89196FR
DyLight 755	NBP2-89196IR
FITC	NBP2-89196F
HRP	NBP2-89196H
Janelia Fluor 525	NBP2-89196JF525
Janelia Fluor 549	NBP2-89196JF549
Janelia Fluor 646	NBP2-89196JF646
Janelia Fluor 669	NBP2-89196JF669
PE	NBP2-89196PE
PerCP	NBP2-89196PCP
mFluor Violet 450 SE	NBP2-89196MFV450
mFluor Violet 500 SE	NBP2-89196MFV500
mFluor Violet 610 SE	NBP2-89196MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-89196-50ul

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NBP2-59057

SOX2 Antibody (CL4716) - BSA Free

100 ul

NBP2-59057

Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of human placenta shows no positivity as expected (negative control).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44099AF350
Alexa Fluor 405	NBP3-44099AF405
Alexa Fluor 488	NBP3-44099AF488
Alexa Fluor 532	NBP3-44099AF532
Alexa Fluor 594	NBP3-44099AF594
Alexa Fluor 647	NBP3-44099AF647
Alexa Fluor 700	NBP3-44099AF700
Alexa Fluor 750	NBP3-44099AF750
Alexa Fluor Plus 405	NBP3-44099AFP405
Alexa Fluor Plus 488	NBP3-44099AFP488
Alexa Fluor Plus 555	NBP3-44099AFP555
Alexa Fluor Plus 594	NBP3-44099AFP594
Alexa Fluor Plus 647	NBP3-44099AFP647
Alexa Fluor Plus 680	NBP3-44099AFP680
Allophycocyanin	NBP3-44099APC
Biotin	NBP3-44099B
CoraFluor 1	NBP3-44099CL1
DyLight 350	NBP3-44099UV
DyLight 405	NBP3-44099V
DyLight 488	NBP3-44099G
DyLight 550	NBP3-44099R
DyLight 594	NBP3-44099DL594
DyLight 650	NBP3-44099C
DyLight 680	NBP3-44099FR
DyLight 755	NBP3-44099IR
FITC	NBP3-44099F
HRP	NBP3-44099H
Janelia Fluor 525	NBP3-44099JF525
Janelia Fluor 549	NBP3-44099JF549
Janelia Fluor 646	NBP3-44099JF646
Janelia Fluor 669	NBP3-44099JF669
PE	NBP3-44099PE
PerCP	NBP3-44099PCP
mFluor Violet 450 SE	NBP3-44099MFV450
mFluor Violet 500 SE	NBP3-44099MFV500
mFluor Violet 610 SE	NBP3-44099MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44099
BSA Free	NBP2-59057-25ul

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NBP3-31961

SOX2 Antibody - BSA Free

500 ul

NBP3-31961

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Conjugate:

Unconjugated

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NBP2-58318

SOX2 Antibody - BSA Free

100 ul

NBP2-58318

ChIP-Exo-Seq composite graph for Anti-SOX2 (NBP2-58318) tested in NCCIT cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

SOX2 Products By Gene Name

SOX2 Antibody [Unconjugated]

SOX2 Antibody (245610) [Unconjugated]

SOX2 Antibody - BSA Free

SOX2 Antibody (4G8) - BSA Free

SOX2 Antibody

SOX2 Antibody (SOX2/1791)

SOX2 Antibody (SOX2/1792)

SOX2 Antibody (PO00-28)

SOX2 Antibody (017) - Azide and BSA Free

SOX2 Antibody (CL4716) - BSA Free

SOX2 Antibody - BSA Free

SOX2 Antibody - BSA Free