CD133 Antibody


Western Blot: CD133 Antibody [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunohistochemistry-Paraffin: CD133 Antibody [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Chromatin Immunoprecipitation: CD133 Antibody [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Flow Cytometry: CD133 Antibody [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 more

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

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CD133 Antibody Summary

Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Stem Cell Marker
CD133 - Hematopoietic Stem Cell Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation
  • ELISA 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Read Publications using
NB120-16518 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for CD133 Antibody

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1


Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
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Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD133 Antibody (NB120-16518)(22)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: ELISA, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB120-16518 Applications Species
Liu J, Dong X, Li Z et al. Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1alpha and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat Model Evid Based Complement Evid Based Complement Alternat Med. [PMID: 32328124] (Flow, Rat) Flow Rat
Qi L, Ahmadi AR, Huang J et al. Major Improvement in Wound Healing Through Pharmacologic Mobilization of Stem Cells in Severely Diabetic Rats Diabetes Jan 23 2020 [PMID: 31974141]
Zhao F, Zhou L, Liu J et al. Construction of a vascularized bladder with autologous adipose-derived stromal vascular fraction cells combined with bladder acellular matrix via tissue engineering J Tissue Eng. 2019 Nov 29 [PMID: 31827758] (Flow, Rat)

Citation used the PE format of this antibody.
Flow Rat
Aghajani Nargesi A, Zhu XY, Liu Y et al. Renal Artery Stenosis Alters Gene Expression in Swine Scattered Tubular-Like Cells Int J Mol Sci Oct 12 2019 [PMID: 31614781] (Flow, ICC/IF, Human) Flow, ICC/IF Human
Aghajani Nargesi A, Zhu XY, Conley SM et al. Renovascular disease induces mitochondrial damage in swine scattered tubular cells Am. J. Physiol. Renal Physiol. Aug 28 2019 [PMID: 31461348] (Porcine) Porcine
Uygur B, Leikina E, Melikov K et al. Interactions with muscle cells boost fusion, stemness and drug resistance of prostate cancer cells Mol. Cancer Res. Dec 26 2018 [PMID: 30587522] (IHC-P, Human) IHC-P Human
Fu JJ, Zhou Y, Shi XX et al. Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment. Bioanalysis. Feb 1 2018 [PMID: 30481703] (FLOW, Human) FLOW Human
Bhattacharyya S, Mitra D, Ray S et al. Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression. Microvascular Research. Jan 1 2019 [PMID: 30381268] (Flow, Mouse) Flow Mouse
Yang Z, Zhang C, Qi W et al. GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFkB in colorectal adenocarcinoma Exp. Cell Res. Oct 12 2018 [PMID: 30321514] (IHC, Human) IHC Human
Bak D, Choi M, Kim S et al. Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth Korean J Physiol Pharmacol Aug 29 2018 [PMID: 30181702] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Show All 22 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for CD133 Antibody (NB120-16518) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin NB120-16518B
DyLight 488 NB120-16518G
DyLight 550 NB120-16518R
DyLight 650 NB120-16518C
PE NB120-16518PE

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Array NB120-16518

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Gene Symbol PROM1