CD133 Antibody - BSA Free


Western Blot: CD133 Antibody - BSA Free [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunocytochemistry/ Immunofluorescence: CD133 Antibody - BSA Free [NB120-16518] - Characterization of Scattered tubular-like cells (STCs) isolated from pig kidneys. A) Representative immunofluorescence staining more
Immunohistochemistry: CD133 Antibody - BSA Free [NB120-16518] - Immunostaining for CD133 (NB120-16518) in normal ovarian and tumor tissue sections. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals more
Flow Cytometry: CD133 Antibody - BSA Free [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained more
Chromatin Immunoprecipitation: CD133 Antibody - BSA Free [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Immunohistochemistry-Paraffin: CD133 Antibody - BSA Free [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, ChIP
BSA Free
1.0 mg/ml

Order Details

CD133 Antibody - BSA Free Summary

Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Stem Cell Marker
CD133 - Hematopoietic Stem Cell Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 1:500-1:1000
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Read Publications using
NB120-16518 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for CD133 Antibody - BSA Free

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1


Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Bv, Ch, Eq, Gp, Hu, Mu, Po, Rb, Rt
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Applications: WB, ELISA, Flow, ICC/IF, IHC, ChIP

Publications for CD133 Antibody (NB120-16518)(31)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 7 applications: ELISA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using NB120-16518 Applications Species
Zhou L, Yang T, Zhao F Et al. Effect of uncultured adipose-derived stromal vascular fraction on preventing urethral stricture formation in rats Sci Rep 2022-03-05 [PMID: 35246575] (FLOW, Rat)

Citation using the PE version of this antibody.
Pomares, A Tumorspheres as an in vitro model for cancer stem-like cell characterization in non-small cell lung cancer. Prognostic implications. Thesis 2020-01-01 (ICC/IF) ICC/IF
King P, Wan J, Guo AA et al. Regulation of gliomagenesis and stemness through acid sensor ASIC1a International journal of oncology 2021-10-01 [PMID: 34515325] (FLOW, Human) FLOW Human
Madlala SC The role of stem cells and WNT signalling pathway in renal cell carcinoma Thesis 2020-01-01 (IHC-P, Human) IHC-P Human
Wamba B, Ghosh P, Mbaveng A, et al. Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies Evid Based Complement Alternat Med 2021-05-19 [PMID: 34007300]
Abouelkheir M, Shabaan DA, Shahien MA Delayed blockage of prostaglandin EP4 receptors can reduce dedifferentiation, epithelial-to-mesenchymal transition and fibrosis following acute kidney injury Clinical and experimental pharmacology & physiology 2021-02-25 [PMID: 33634509]
Wan J, Guo A, King P et al. TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma Frontiers in Pharmacology 2020-12-14 [PMID: 33381038] (WB, Human) WB Human
Horiguchi K, Yoshida S, Tsukada T et al. Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells J. Reprod. Dev. 2020-08-21 [PMID: 32830152]
Harper S, Hoff M, Skepper J et al. Portal venous repopulation of decellularised rat liver scaffolds with syngeneic bone marrow stem cells J Tissue Eng Regen Med 2020-08-17 [PMID: 32808475]
Liu J, Dong X, Li Z et al. Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1alpha and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat Model Evid Based Complement Evid Based Complement Alternat Med. [PMID: 32328124] (FLOW, Rat) FLOW Rat
Show All 31 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


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Array NB120-16518

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
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Gene Symbol PROM1