CD133 Antibody

Images

 
Western Blot: CD133 Antibody [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunohistochemistry-Paraffin: CD133 Antibody [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Chromatin Immunoprecipitation: CD133 Antibody [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Flow Cytometry: CD133 Antibody [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates

CD133 Antibody Summary

Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation
  • ELISA 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Publications
Read Publications using
NB120-16518 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD133 Antibody

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD133 Antibody (NB120-16518)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: ELISA, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
Flow
(2)
ICC/IF
(5)
IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(8)
Mouse
(3)
Rat
(2)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB120-16518 Applications Species
Uygur B, Leikina E, Melikov K et al. Interactions with muscle cells boost fusion, stemness and drug resistance of prostate cancer cells Mol. Cancer Res. Dec 26 2018 [PMID: 30587522] (IHC-P, Human) IHC-P Human
Fu JJ, Zhou Y, Shi XX et al. Spontaneous formation of tumor spheroid on a hydrophilic filter paper for cancer stem cell enrichment. Bioanalysis. Feb 1 2018 [PMID: 30481703] (FLOW, Human) FLOW Human
Bhattacharyya S, Mitra D, Ray S et al. Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression. Microvascular Research. Jan 1 2019 [PMID: 30381268] (Flow, Mouse) Flow Mouse
Yang Z, Zhang C, Qi W et al. GLI1 promotes cancer stemness through intracellular signaling pathway PI3K/Akt/NFkB in colorectal adenocarcinoma Exp. Cell Res. Oct 12 2018 [PMID: 30321514] (IHC, Human) IHC Human
Bak D, Choi M, Kim S et al. Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth Korean J Physiol Pharmacol Aug 29 2018 [PMID: 30181702] (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Parte SC, Batra SK, Kakar SS. Characterization of stem cell and cancer stem cell populations in ovary and ovarian tumors J Ovarian Res Aug 18 2018 [PMID: 30121075] (ICC/IF, IHC, Human) ICC/IF, IHC Human
Li H, Meng QH, Noh H et al. Cell-surface vimentin-positive macrophage-like circulating tumor cells as a novel biomarker of metastatic gastrointestinal stromal tumors Oncoimmunology Jan 8 2018 [PMID: 29721368] (ICC/IF, Human) ICC/IF Human
Kim MS, Cho HI, Yoon HJ et al. JIB-04, A Small Molecule Histone Demethylase Inhibitor, Selectively Targets Colorectal Cancer Stem Cells by Inhibiting the Wnt/B-Catenin Signaling Pathway Sci Rep Apr 26 2018 [PMID: 29700375] (WB, Human) WB Human
Zou X, Kwon SH, Jiang K et al. Renal scattered tubular-like cells confer protective effects in the stenotic murine kidney mediated by release of extracellular vesicles Sci Rep 2018 Jan 19 [PMID: 29352176] (IHC-Fr, Porcine) IHC-Fr Porcine
Alhaider IA, Mohamed ME, Ahmed KKM, Kumar AHS. Date Palm (Phoenix dactylifera) Fruits as a Potential Cardioprotective Agent: The Role of Circulating Progenitor Cells Front Pharmacol 2017 Sep 20 [PMID: 28928656] (Flow, Rat) Flow Rat
Show All 16 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for CD133 Antibody (NB120-16518) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin NB120-16518B
DyLight 488 NB120-16518G
DyLight 550 NB120-16518R
DyLight 650 NB120-16518C
PE NB120-16518PE

Additional CD133 Products

Array NB120-16518

Bioinformatics Tool for CD133 Antibody (NB120-16518)

Discover related pathways, diseases and genes to CD133 Antibody (NB120-16518). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for CD133 Antibody (NB120-16518)

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

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Bioinformatics

Gene Symbol PROM1