CD133 Antibody - BSA Free

Images

 
Western Blot: CD133 Antibody [NB120-16518] - Predicted band size : 97 kDa recognizes 97 kDa human CD133 in Y79 cells. Band is at ~120 kDa due to protein glycosylation.
Immunohistochemistry-Paraffin: CD133 Antibody [NB120-16518] - Staining of CD133 in human hepatocarcinoma (HC) tissue. NB120-16518 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Chromatin Immunoprecipitation: CD133 Antibody [NB120-16518] - [NB 120-16518] - Staining CD133 in Bone Marrow Mononuclear Cells by Immunofluorescence.
Flow Cytometry: CD133 Antibody [NB120-16518] - Flow Cytometry: CD133 Antibody [DyLight 488] [NB120-16518G] - Flow Cytometry: CD133 Antibody (DyLight488) [NB120-16518G] - Rat bone marrow cells were stained with CD133 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD133 Antibody - BSA Free Summary

Immunogen
Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865).
Marker
Stem Cell Marker
Specificity
CD133 - Hematopoietic Stem Cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PROM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • ELISA 1:1000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100-1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Western Blot 1:500-1:1000
Application Notes
Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). IHC: Citrate buffer antigen retreival required. ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT. Optimal dilutions/concentrations should be determined by the end user. Use in IHC-Frozen reported in scientific literature (PMID: 29352176).
Publications
Read Publications using
NB120-16518 in the following applications:

Reactivity Notes

porcine reactivity reported in scientific literature (PMID: 29352176).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD133 Antibody - BSA Free

  • AC133 Stargardt disease 4 (autosomal dominant)
  • AC133
  • CD133 retinal 2
  • CD133
  • PROM1
  • prominin (mouse)-like 1
  • Prominin 1
  • PROML1

Background

Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CD133 Antibody (NB120-16518)(30)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 8 applications: ELISA, FLOW, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
FLOW
(2)
Flow
(6)
ICC/IF
(8)
IHC
(6)
IHC-Fr
(1)
IHC-P
(2)
WB
(5)
All Applications
Filter By Species
Human
(13)
Mouse
(4)
Rat
(4)
Porcine
(2)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB120-16518 Applications Species
King P, Wan J, Guo AA et al. Regulation of gliomagenesis and stemness through acid sensor ASIC1a International journal of oncology Oct 1 2021 [PMID: 34515325] (Flow, Human) Flow Human
Abouelkheir M, Shabaan DA, Shahien MA Delayed blockage of prostaglandin EP4 receptors can reduce dedifferentiation, epithelial-to-mesenchymal transition and fibrosis following acute kidney injury Clinical and experimental pharmacology & physiology Feb 25 2021 [PMID: 33634509]
Aghajani Nargesi A, Zhu XY, Conley SM et al. Renovascular disease induces mitochondrial damage in swine scattered tubular cells Am. J. Physiol. Renal Physiol. Aug 28 2019 [PMID: 31461348] (Porcine) Porcine
Wamba B, Ghosh P, Mbaveng A, et al. Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies Evidence-Based Complementary and Alternative Medicine Jan 18 2021 (ICC/IF, WB, Mouse) ICC/IF, WB Mouse
Wan J, Guo A, King P et al. TRPM7 Induces Tumorigenesis and Stemness Through Notch Activation in Glioma Frontiers in Pharmacology Dec 14 2020 [PMID: 33381038] (WB, Human) WB Human
Madlala SC The role of stem cells and WNT signalling pathway in renal cell carcinoma Thesis Jan 1 2020 (IHC-P, Human) IHC-P Human
Horiguchi K, Yoshida S, Tsukada T et al. Expression and functions of cluster of differentiation 9 and 81 in rat mammary epithelial cells J. Reprod. Dev. Aug 21 2020 [PMID: 32830152]
Harper S, Hoff M, Skepper J et al. Portal venous repopulation of decellularised rat liver scaffolds with syngeneic bone marrow stem cells J Tissue Eng Regen Med Aug 17 2020 [PMID: 32808475]
Liu J, Dong X, Li Z et al. Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1alpha and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma Rat Model Evid Based Complement Evid Based Complement Alternat Med. [PMID: 32328124] (Flow, Rat) Flow Rat
Pomares, A Tumorspheres as an in vitro model for cancer stem-like cell characterization in non-small cell lung cancer. Prognostic implications. Thesis Jan 1 2020 (ICC/IF) ICC/IF
Show All 30 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for CD133 Antibody (NB120-16518) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NB120-16518

Bioinformatics Tool for CD133 Antibody (NB120-16518)

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Blogs on CD133.

Stemness is responsible for onset and metastasis of colorectal cancer
By Jamshed Arslan, Pharm. D., PhD. Colorectal cancer stem cells are a rare subpopulation of colorectal cancer cells that can self-renew and initiate and sustain tumor growth when transplanted into an animal host.1,2 C...  Read full blog post.

ATG9A - early marker autophagosome assembly
ATG9A is the only essential integral membrane protein involved in autophagy. ATG9A contains six transmembrane domains and initiates the assembly of autophagosomes. The autophagosome is a double-membrane structure that engulfs and eventually degrade...  Read full blog post.

CD133
Also known as PROM1 and AC133, this gene is located on chromosome 4p15 and encodes CD133, a 120kDa pentaspan transmembrane glycoprotein (5-TM) and presents multiple spliced variants. Prominin-1 (CD133) was the first protein identified as "Prominin"; o...  Read full blog post.

CD Antibodies Uncover Markers for Rare Breast Cancer
We at Novus Biologicals have added several new products to our CD antibody database. The CD, or Cluster of Differential proteins are a family of type I transmembrane glycoproteins widely expressed in immune cell populations. These include B cells, thy...  Read full blog post.

Omicron Variant Antibodies

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Bioinformatics

Gene Symbol PROM1