GFAP Antibody


Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - ICC-IF analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Xenografted mouse brain section : astocyte and human nuclei. Image from a confirmed customer review.
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Cultured Rat Hippocampal Neuron, image courtesy of customer.
Simple Western: GFAP Antibody [NB300-141] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa more
Western Blot: GFAP Antibody [NB300-141] - WB analysis of Rat brain lysate using at a dilution of 1:5,000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was more
Western Blot: GFAP Antibody [NB300-141] - Analysis of GFAP expression in whole rat cerebellum homogenate.
Immunohistochemistry-Frozen: GFAP Antibody [NB300-141] - Imaging of mouse brain (cortex), 20x magnification. This image was submitted via customer Review.

Product Details

Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, RbSpecies Glossary
Applications WB, Simple Western, IHC

Order Details

GFAP Antibody Summary

GFAP purified from bovine spinal cord. [UniProt# Q28115]
Astrocyte Marker
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:50000
  • Simple Western 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:1000-1:5000
Application Notes
This GFAP Antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on both paraffin-embedded and frozen sections. In Western blot a band can be seen at 50-55 kDa representing GFAP. A lower band may be seen around 45 kDa representing a proteolytic fragment derived from the GFAP molecule.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
GFAP Lysate (NBL1-11043)
Reviewed Applications
Read 4 Reviews rated 4.3
NB300-141 in the following applications:

Read Publications using
NB300-141 in the following applications:

Reactivity Notes

Human, mouse, rat, chicken, bovine, porcine, rabbit, guinea pig, canine and feline. Predicted to work with most mammals.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Whole antisera
No Preservative

Alternate Names for GFAP Antibody

  • FLJ45472
  • GFAP astrocytes
  • GFAP immunohistochemistry
  • GFAP mouse
  • GFAP rabbit
  • GFAP stain
  • GFAP
  • glial fibrillary acidic protein


GFAP (Glial Fibrillary Acidic Protein) is a member of the class III intermediate filament protein family. It is heavily and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells, in peripheral ganglia and in non-myelinating Schwann cells in peripheral nerves. In addition neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
Species: Hu, Mu, Rt, Po, Bv, Ch, GP, Ma, Rb, Sh
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, RI
Species: Hu, Mu, Sh
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Ch, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Bv
Applications: WB, ICC/IF, IHC, IHC-Fr
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, ICC, IHC-FrFl
Species: Hu, Mu, Rt, Bv, Xp
Applications: WB, Simple Western, ICC/IF
Species: Hu, Mu, Rt, Ca, Eq
Species: Hu, Mu, Rt, Bv
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, IHC, IHC-P
Species: Hu
Applications: IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, GP, Rb
Applications: WB, Simple Western, IHC

Publications for GFAP Antibody (NB300-141)(32)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig, Rabbit.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-FR, IHC-Fr, WB.

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Showing Publications 1 - 10 of 32. Show All 32 Publications.
Publications using NB300-141 Applications Species
Jurga AM, Piotrowska A, Makuch W et al. Blockade of P2X4 Receptors Inhibits Neuropathic Pain-Related Behavior by Preventing MMP-9 Activation and, Consequently, Pronociceptive Interleukin Release in a Rat Model. Front Pharmacol. Mar 9 2017 [PMID: 28275350] (WB, Rat) WB Rat
Fil D, DeLoach A, Yadav S et al. Mutant Profilin1 transgenic mice recapitulate cardinal features of motor neuron disease Hum. Mol. Genet Dec 30 2016 [PMID: 28040732] (IHC-FR, Human) IHC-FR Human
Zychowska M, Rojewska E, Makuch W et al. Participation of pro- and anti-nociceptive interleukins in botulinum toxin A-induced analgesia in a rat model of neuropathic pain. Eur. J. Pharmacol. Sep 9 2016 [PMID: 27619001]
Kenig S, Faoro V, Bourkoula E et al. Topoisomerase IIbeta mediates the resistance of glioblastoma stem cells to replication stress-inducing drugs Cancer Cell Int. 2016 Jul 27 [PMID: 27462186] (WB) WB
Piotrowska A, Kwiatkowski K, Rojewska E. Direct and indirect pharmacological modulation of CCL2/CCR2 pathway results in attenuation of neuropathic pain-In vivo and in vitro evidence. J Neuroimmunol. Aug 15 2016 [PMID: 27397071] (WB, Rat) WB Rat
Pilat D, Piotrowska A, Rojewska E et al. Blockade of IL-18 signaling diminished neuropathic pain and enhanced the efficacy of morphine and buprenorphine. Mol. Cell. Neurosci. 2016 Jan 05 [PMID: 26763728] (WB, Rat) WB Rat
Anzilotti S, Tornincasa M, Gerlini R et al. Genetic ablation of homeodomain-interacting protein kinase 2 selectively induces apoptosis of cerebellar Purkinje cells during adulthood and generates an ataxic-like phenotype. Cell Death Dis. 2015 Dec 04 [PMID: 26633710] (WB, IHC-Fr, Mouse) WB, IHC-Fr Mouse
Kwon JJ, Nabinger SC, Vega Z et al. Pathophysiological role of microRNA-29 in pancreatic cancer stroma. Sci Rep 2015 [PMID: 26095125] (ICC/IF) ICC/IF
Popiolek-Barczyk K, Kolosowska N. Parthenolide Relieves Pain and Promotes M2 Microglia/Macrophage Polarization in Rat Model of Neuropathy. Neural Plasticity. 2015 [PMID: 26090236] (WB, Rat) WB Rat
Northcott PA, Lee C, Zichner T et al. Enhancer hijacking activates GFI1 family oncogenes in medulloblastoma. Nature 2014 Jul 24 [PMID: 25043047]
Show All 32 Publications.

Reviews for GFAP Antibody (NB300-141) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Mouse, Rat.

Reviews using NB300-141:
Filter by Applications
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen GFAP NB300-141
reviewed by:
IHC-Fr Mouse 06/29/2017


Sample TestedPFA Fixed Frozen Section of Mouse Brain


CommentsWD [1:K]; Secondary ab RTU Polymer-HRP
reviewed by:
ICC Mouse 04/18/2017


Sample TestedPrimary mouse cortical astrocytes
Immunocytochemistry GFAP NB300-141
reviewed by:
Janosch Heller
ICC Rat 03/27/2017


Sample TestedPrimary rat cortical neurons


CommentsFixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
Blocking: 10% donkey serum and 1% BSA in PBS with 0.1% saponin, 1 hour RT
Permeabilization: 0.1% saponin
Primary Antibody: 1:1000 in PBS with 0.1% saponin, overnight, 4°C
Wash: three times wash with PBS with 0.1% saponin, 10 min each
Secondary Antibody: Invitrogen goat anti-rabbit Alexa647, 1:500 in PBS with 0.1% saponin, 2 hours, RT
Wash: two washes with PBS with 0.1% saponin, 10 min each and three washes with PBS, 10 min each
Microscope: Burker Vutara 350

The antibody labels intermediate filaments in cultured astrocytes.
Immunofluorescence GFAP NB300-141
reviewed by:
IF Mouse 01/17/2014


Pub Med ID23949222
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFAP Antibody (NB300-141). (Showing 1 - 3 of 3 FAQs).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.
  2. Our customer would like to know if your antibody is suitable for frozen sample by IF.
    • NB300-141 has been tested and validated in IHC-Frozen and IF; this is covered by our guarantee.
  3. I would like to use your GFAP-antibody (cat # NB300-141) in a Western Blot experiment. Could you tell me the dilution of this antibody?
    • The dilution recommended for Western blot using NB300-141 is 1:50,000.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on GFAP.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

GFAP: Roles in Alzheimer's and Schizophrenia
Glial fibrillary acidic protein (GFAP) is a class III intermediate filament (IF) protein and is used as a marker to distinguish astrocytes from other glial cells during development. GFP may play a role in maintaining mechanical strength and shape in a...  Read full blog post.

Vimentin in Wound Healing
Vimentin is a fundamental 10 nm type III intermediate filament (IF) protein found in many mesenchymal and epithelia tissues, tissue culture cells, and developing neuronal and astrocytic precursor cells of the central nervous system. It frequently co-p...  Read full blog post.

"Whats the Hap" with GFAP?
Glial Fibrillary Acidic Protein (GFAP) is one of the major intermediate filament axonal proteins found in mature astrocytes, the star-shaped glial cells that comprise the majority of cells within the central nervous system (1). Astrocytes perform a wi...  Read full blog post.

GFAP Antibodies are a 'No Brainer' for Neurodegenerative Research
Glial fibrillary acidic protein (GFAP) is the main intermediate filament protein in mature astrocytes, but also an important component of the cytoskeleton in astrocytes during development. Recent developments using GFAP antibodies in astrocyte biology...  Read full blog post.

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Recent Reviews


Application: IHC-Fr
Species: Mouse

Application: ICC
Species: Mouse

Janosch Heller
Application: ICC
Species: Rat


Gene Symbol GFAP