Reactivity | Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, RbSpecies Glossary |
Applications | WB, Simple Western, ICC/IF, IHC |
Clonality | Polyclonal |
Host | Rabbit |
Conjugate | Unconjugated |
Immunogen | This GFAP Antibody was developed against recombinant full length human GFAP isotype 1 expressed in and purified from E. coli. |
Marker | Astrocyte Marker |
Clonality | Polyclonal |
Host | Rabbit |
Gene | GFAP |
Purity | Unpurified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | In WB a band can be seen at 50-55 kDa representing GFAP. A lower band may be seen around 45 kDa representing a proteolytic fragment derived from the GFAP molecule. GFAP antibody validated for IHC-P from a verified customer review. IHC-Fr has been reported in scientific literature (PMID: 28040732). Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50-1:2000; matrix was 12-230 kDa. |
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Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | Supplied as serum |
Preservative | 5mM Sodium Azide |
Purity | Unpurified |
Publications using NB300-141 | Applications | Species |
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chen p, Lin M, Chen R et al. NeuroD1 improved neurocognitive function in a mouse model of subarachnoid hemorrhage Research Square 2023-04-03 (IHC, Mouse) | IHC | Mouse |
Ciechanowska A, Rojewska E, Piotrowska A et al. New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies Frontiers in immunology 2022-12-22 [PMID: 36618360] (WB, Mouse) | WB | Mouse |
Richards K, Coulthard L, Rome C et al. Glass-brain mapping provides an adjunct tool for structural analysis in mouse models of neurodevelopmental disease Neuroimage: Reports 2021-06-01 | ||
Liao Z, Wang W, Deng W et al. Human Umbilical Cord Mesenchymal Stem Cells-Secreted TSG-6 Is Anti-Inflammatory and Promote Tissue Repair After Spinal Cord Injury ASN Neuro 2021-01-01 (IF/IHC, Rat) | IF/IHC | Rat |
Chang CY, Hung JH, Wu CC et al. Enhancer regulation for induced WNT3A expression during neuronal regeneration bioRxiv 2019-12-02 (Chemotaxis, Rat) | Chemotaxis | Rat |
Williams EP, Xue Y, Lee J et al. Deep spatial profiling of Venezuelan equine encephalitis virus reveals increased genetic diversity amidst neuroinflammation and cell death during brain infection Journal of Virology 2023-08-31 [PMID: 37560924] | ||
Poelaert KCK, van Kleef RGDM, Liu M et al. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences mBio 2023-03-06 [PMID: 36877033] (ICC/IF, Rat) | ICC/IF | Rat |
Pawlik K, Ciapa?a K, Ciechanowska A et al. Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain Cells 2022-12-26 [PMID: 36611891] | ||
Chen P, Liu X, Lin M et al. NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage Research Square 2023-06-30 (IHC-Fr, Mouse) | IHC-Fr | Mouse |
Show All 97 Publications. |
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WB | Bovine | 07/28/2020 |
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WB | Goat | 07/28/2020 |
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WB | Pig | 07/28/2020 |
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WB | Human | 07/28/2020 |
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WB | Mouse | 07/28/2020 |
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WB | Rat | 07/28/2020 |
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ICC | Human | 06/23/2020 |
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IHC-P | Mouse | 06/07/2019 |
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IHC | Mouse | 06/29/2017 |
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ICC | Mouse | 04/18/2017 |
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Janosch Heller |
ICC | Rat | 03/27/2017 |
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IF | Mouse | 01/17/2014 |
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Secondary Antibodies |
Isotype Controls |
Diseases for GFAP Antibody (NB300-141)Discover more about diseases related to GFAP Antibody (NB300-141).
| Pathways for GFAP Antibody (NB300-141)View related products by pathway.
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PTMs for GFAP Antibody (NB300-141)Learn more about PTMs related to GFAP Antibody (NB300-141).
| Research Areas for GFAP Antibody (NB300-141)Find related products by research area.
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Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera... Read full blog post. |
Astrocytes: Diversity in type and function By Michalina Hanzel, PhDAstrocytes are a type of macroglia found in the central nervous system that regulate a vast array of essential brain functions, ranging from synaptogenesis, ion homeostasis and maintenance of t... Read full blog post. |
Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ... Read full blog post. |
Deriving neural precursor cells from human induced pluripotent stem cells By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions... Read full blog post. |
Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by ... Read full blog post. |
Beta Tubulin III and neurogenesis Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr... Read full blog post. |
The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2 Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain. Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa). The importance of t... Read full blog post. |
Synapsin I: Implicated in synaptic activity across a diverse range of studies Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal. Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i... Read full blog post. |
Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g... Read full blog post. |
Nestin: Investigating the Link Between New Brain Cells and Depression Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Verified Customer 07/28/2020 |
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Application: | WB | |
Species: | Bovine |
Verified Customer 07/28/2020 |
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Application: | WB | |
Species: | Goat |
Verified Customer 07/28/2020 |
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Application: | WB | |
Species: | Pig |