GFAP Antibody

Images

 
Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, ...read more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).
Simple Western: GFAP Antibody [NB300-141] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - The inhibition of Kv1.3 channels induces neuroprotection against the toxic effects of glioma. Immunofluorescence analyses of Kv1.3 expression (red) on ...read more
Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141] - Mouse brain section, 20x magnification. Antibody at 1:1000. Detection with Polymer-HRP. IHC image submitted by a verified customer review.
Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.
Western Blot: GFAP Antibody [NB300-141] - Analysis of GFAP expression in whole rat cerebellum homogenate.
Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal ...read more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain ...read more
Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Cultured Rat hippocampal neurons. ICC/IF image submitted by a verified customer review.
Immunohistochemistry: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Frozen: GFAP Antibody [NB300-141] - Imaging of mouse brain (cortex), 20x magnification. IHC image submitted by a verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

GFAP Antibody Summary

Immunogen
This GFAP Antibody was developed against recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Marker
Astrocyte Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFAP
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Simple Western 1:10000
  • Western Blot 1:5000
Application Notes
In WB a band can be seen at 50-55 kDa representing GFAP. A lower band may be seen around 45 kDa representing a proteolytic fragment derived from the GFAP molecule. GFAP antibody validated for IHC-P from a verified customer review. IHC-Fr has been reported in scientific literature (PMID: 28040732).

Simple Western reported by an internal validation. Separated by Size-All, antibody dilution of 1:50-1:2000; matrix was 12-230 kDa.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
GFAP Overexpression Lysate
Reviewed Applications
Read 12 Reviews rated 4.7
using
NB300-141 in the following applications:

Publications
Read Publications using
NB300-141 in the following applications:

Reactivity Notes

Predicted to work with most mammals. Chicken reactivity reported in scientific literature (PMID: 20844134). Rabbit and Guinea Pig reactivity reported in scientific literature (PMID: 4559710).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Supplied as serum
Preservative
5mM Sodium Azide
Purity
Unpurified

Alternate Names for GFAP Antibody

  • ALXDRD
  • FLJ45472
  • GFAP astrocytes
  • GFAP immunohistochemistry
  • GFAP mouse
  • GFAP rabbit
  • GFAP stain
  • GFAP
  • glial fibrillary acidic protein

Background

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFAP Antibody (NB300-141)(80)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Chicken, Guinea Pig, Rabbit.

We have publications tested in 7 applications: ChIP, ICC/IF, IHC, IHC-FR, IHC-Fr, IHC-P, WB.


Filter By Application
ChIP
(1)
ICC/IF
(23)
IHC
(25)
IHC-FR
(1)
IHC-Fr
(5)
IHC-P
(2)
WB
(21)
All Applications
Filter By Species
Human
(9)
Mouse
(45)
Rat
(21)
Chicken
(1)
Guinea Pig
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using NB300-141 Applications Species
Vegh C, Wear D, Okaj I et al. Combined Ubisol-Q10 and Ashwagandha Root Extract Target Multiple Biochemical Mechanisms and Reduces Neurodegeneration in a Paraquat-Induced Rat Model of Parkinsons Disease Antioxidants Apr 6 2021 [PMID: 33917328] (IHC, Rat) IHC Rat
Murtaj V, Belloli S, Di Grigoli G et al. Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge Front Aging Neurosci Nov 5 2019 [PMID: 31749696] (IHC-Fr, Mouse) IHC-Fr Mouse
Mazur C, Powers B, Zasadny K et al. Brain pharmacology of intrathecal antisense oligonucleotides revealed through multimodal imaging JCI Insight Oct 17 2019 [PMID: 31619586] (IHC-Fr, Rat) IHC-Fr Rat
Piotrowska A, Rojewska E, Pawlik K et al. Pharmacological Blockade of Spinal CXCL3/CXCR2 Signaling by NVP CXCR2 20, a Selective CXCR2 Antagonist, Reduces Neuropathic Pain Following Peripheral Nerve Injury Front Immunol 2019 Sep 26 [PMID: 31616413] (WB, Rat, Mouse) WB Rat, Mouse
McAvoy KM, Rajamohamed Sait H, Marsh G et al. Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo PLoS ONE Aug 13 2019 [PMID: 31408457] (ICC/IF, Rat, Mouse) ICC/IF Rat, Mouse
de Carvalho T, Peixoto C, de Paiva I et al. Does physical exercise influence in the development of Neuroeschistosomiasis? Brain Research Bulletin Aug 1 2019 [PMID: 31377443] (IHC, Mouse) IHC Mouse
FranCa MER, Ramos RKLG, Oliveira WH et al. Tadalafil restores long-term memory and synaptic plasticity in mice with hepatic encephalopathy Toxicol. Appl. Pharmacol. Jul 16 2019 [PMID: 31323263] (IHC-P, Mouse) IHC-P Mouse
Crotti A, Sait HR, McAvoy KM et al. BIN1 favors the spreading of Tau via extracellular vesicles Sci Rep Jul 1 2019 [PMID: 31263146] (IHC, Mouse) IHC Mouse
Gimenes AD, Andrade BFD, Pinotti JVP et al. Annexin A1-derived peptide Ac2-26 in a pilocarpine-induced status epilepticus model: anti-inflammatory and neuroprotective effects J Neuroinflammation. 2019 Feb 11 [PMID: 30755225] (WB, Rat) WB Rat
Sankar SB, Pybus AF, Liew A et al. Low cerebral blood flow is a non-invasive biomarker of neuroinflammation after repetitive mild traumatic brain injury Neurobiol. Dis. Dec 25 2018 [PMID: 30592976] (WB, Mouse) WB Mouse
Show All 80 Publications.

Reviews for GFAP Antibody (NB300-141) (12) 4.712

Average Rating: 4.7
(Based on 12 reviews)
We have 12 reviews tested in 6 species: Human, Mouse, Rat, Bovine, Goat, Pig.

Reviews using NB300-141:
Filter by Applications
WB
(6)
ICC
(3)
IHC-P
(1)
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(5)
Rat
(2)
Bovine
(1)
Goat
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GFAP NB300-141
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4
reviewed by:
Verified Customer
WB Bovine 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesBovine
Lot179040517
Western Blot GFAP NB300-141
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5
reviewed by:
Verified Customer
WB Goat 07/28/2020
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Summary

ApplicationWestern Blot
Sample Tested30ug,retina
SpeciesGoat
Lot179040517
Western Blot GFAP NB300-141
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5
reviewed by:
Verified Customer
WB Pig 07/28/2020
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Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesPig
Lot179040517

Comments

Commentsuse at 1:500
Western Blot GFAP NB300-141
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5
reviewed by:
Verified Customer
WB Human 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesHuman
Lot179040517
Western Blot GFAP NB300-141
Enlarge
5
reviewed by:
Verified Customer
WB Mouse 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesMouse
Lot179040517
Western Blot GFAP NB300-141
Enlarge
5
reviewed by:
Verified Customer
WB Rat 07/28/2020
View

Summary

ApplicationWestern Blot
Sample Testedretina
SpeciesRat
Lot179-040517
Immunocytochemistry GFAP NB300-141
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5
reviewed by:
Verified Customer
ICC Human 06/23/2020
View

Summary

ApplicationImmunocytochemistry
Sample Testedmixed neuronal primary culture
SpeciesHuman
Lot179040517
Immunohistochemistry-Paraffin GFAP NB300-141
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5
reviewed by:
Verified Customer
IHC-P Mouse 06/07/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Tested Mouse brain
SpeciesMouse
Lot121712

Comments

CommentsProteinasi K treatment; Primary Ab 1:1000. Detection with Polymer-HRP.
Immunohistochemistry-Frozen GFAP NB300-141
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4
reviewed by:
Verified Customer
IHC-Fr Mouse 06/29/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedPFA Fixed Frozen Section of Mouse Brain
SpeciesMouse

Comments

CommentsWD [1:K]; Secondary ab RTU Polymer-HRP
  3
reviewed by:
Verified Customer
ICC Mouse 04/18/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary mouse cortical astrocytes
SpeciesMouse
Immunocytochemistry GFAP NB300-141
Enlarge
5
reviewed by:
Janosch Heller
ICC Rat 03/27/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedPrimary rat cortical neurons
SpeciesRat

Comments

CommentsFixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
Blocking: 10% donkey serum and 1% BSA in PBS with 0.1% saponin, 1 hour RT
Permeabilization: 0.1% saponin
Primary Antibody: 1:1000 in PBS with 0.1% saponin, overnight, 4°C
Wash: three times wash with PBS with 0.1% saponin, 10 min each
Secondary Antibody: Invitrogen goat anti-rabbit Alexa647, 1:500 in PBS with 0.1% saponin, 2 hours, RT
Wash: two washes with PBS with 0.1% saponin, 10 min each and three washes with PBS, 10 min each
Microscope: Burker Vutara 350

The antibody labels intermediate filaments in cultured astrocytes.
Immunofluorescence GFAP NB300-141
Enlarge
5
reviewed by:
Verified Customer
IF Mouse 01/17/2014
View

Summary

ApplicationImmunofluorescence
SpeciesMouse
Pub Med ID23949222
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFAP Antibody (NB300-141). (Showing 1 - 3 of 3 FAQs).

  1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
    • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.
  2. Our customer would like to know if your antibody is suitable for frozen sample by IF.
    • NB300-141 has been tested and validated in IHC-Frozen and IF; this is covered by our guarantee.
  3. I would like to use your GFAP-antibody (cat # NB300-141) in a Western Blot experiment. Could you tell me the dilution of this antibody?
    • The dilution recommended for Western blot using NB300-141 is 1:50,000.

Control Lysate(s)

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Blogs on GFAP. Showing 1-10 of 14 blog posts - Show all blog posts.

Successful Transplantation of Friedreich Ataxia Induced Pluripotent Stem Cell (iPSC)-Derived Sensory Neurons in Dorsal Root Ganglia of Adult Rodents
Jamshed Arslan, Pharm D, PhD The dorsal root ganglia (DRG) are a collection of cell bodies of sensory nerves carrying sensory information – including nociception, mechanoreception and proprioception – from periphera...  Read full blog post.

Astrocytes: Diversity in type and function
By Michalina Hanzel, PhDAstrocytes are a type of macroglia found in the central nervous system that regulate a vast array of essential brain functions, ranging from synaptogenesis, ion homeostasis and maintenance of t...  Read full blog post.

Role of GFAP in astrocytes: Lessons from induced pluripotent stem cells in Alexander disease patients
By Michalina Hanzel, PhDAlexander disease is a progressive and fatal neurological disease with phenotypes ranging from myelination abnormalities, gait ataxia and megalencephaly to predisposition to seizures. It is an ...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Identifying tumoral and stromal transcriptomes that underlie tumor plasticity and stromal neuroinflammatory response in brain metastasis
By Jamshed Arslan, Pharm. D., PhD. Cancers in the brain often come from tumors elsewhere in the body. Several adaptive mechanisms influence brain metastasis, such as blood brain barrier leakage that can be induced by ...  Read full blog post.

Beta Tubulin III and neurogenesis
Beta tubulin III, also known as Tuj-1, is a class III member of the beta tubulin protein family. Beta tubulins are one of two structural components that form our microtubule network. While general tubulins play a role in a wide range of cellular pr...  Read full blog post.

The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

Synapsin I: Implicated in synaptic activity across a diverse range of studies
Synapsins are a family of neuronal proteins that are most renowned for their activity in modulating the pre-synaptic terminal.  Synapsin’s behavior is regulated by protein kinases and phosphatases, which alter the way that synapsin’s i...  Read full blog post.

Glial Fibrillary Acidic Protein (GFAP), The Most Popular Astrocyte Marker
GFAP, a class-III intermediate filament, is a 50kDa protein which is found in the mature and developing astrocytes in the CNS, non-myelinating Schwann cells in the PNS, enteric glial cells (enteric nervous system/ENS), ependymal cells, and radial g...  Read full blog post.

Nestin: Investigating the Link Between New Brain Cells and Depression
Clinical depression (also known as major depressive disorder or MDD) affects many people, but the biological processes that cause it (and are influenced by its treatments) are not well understood. Adult neurogenesis is a newly emerging field that coul...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.
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Recent Reviews

4.7
5
9
4
2
3
1
2
0
1
0

Verified Customer
07/28/2020
Application: WB
Species: Bovine

Verified Customer
07/28/2020
Application: WB
Species: Goat

Verified Customer
07/28/2020
Application: WB
Species: Pig

Bioinformatics

Gene Symbol GFAP
Entrez
Uniprot