GFAP Antibody

Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).

Simple Western: GFAP Antibody [NB300-141] - Simple Western lane view shows a specific band for GFAP in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - The inhibition of Kv1.3 channels induces neuroprotection against the toxic effects of glioma. Immunofluorescence analyses of Kv1.3 expression (red) on GFAP-positive cells (green) in coronal brain slices of GL261-bearing mice in peritumoral region; in the magnification (right) arrows indicate co-expression of Kv1.3 and GFAP in the same cell. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25940-5) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: GFAP Antibody [NB300-141] - Mouse brain section, 20x magnification. Antibody at 1:1000. Detection with Polymer-HRP. IHC image submitted by a verified customer review.

Western Blot: GFAP Antibody [NB300-141] - Analysis of Rat brain lysate. Antibody at 1:5000. Specific band running with an apparent SDS-PAGE molecular weight of ~50 kDa corresponds to rodent GFAP was observed.

Western Blot: GFAP Antibody [NB300-141] - Analysis of GFAP expression in whole rat cerebellum homogenate.

Western Blot: GFAP Antibody [NB300-141] - Analysis of different tissue lysates using rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. Strong band at about 50 kDa corresponds to the major isotype of the GFAP protein. Smaller isotypes and proteolytic fragments of GFAP are also detected on the blot.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Analysis of mixed neuron-glial cultures using GFAP antibody NB300-141 (red) and Vimentin antibody NB300-223 (green). The fibroblastic cells contain only Vimentin and so are green. The astrocytes contain either Vimentin and GFAP (appearing golden) or predominantly GFAP (appearing red). Blue is nuclear DNA stain.

Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Cultured Rat hippocampal neurons. ICC/IF image submitted by a verified customer review.

Immunohistochemistry: GFAP Antibody [NB300-141] - Xenografted mouse brain section: astocyte and human nuclei. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Frozen: GFAP Antibody [NB300-141] - Imaging of mouse brain (cortex), 20x magnification. IHC image submitted by a verified customer review.

EGFP+ neurons are positive for RABV antigen.Brains were collected from Cre reporter mice fifteen days post-infection, cryosectioned, and EGFP+ regions compared to cell-specific labeling, A) NeuN (blue, neuronal nuclei antibody, 20× fluorescence imaging), B) GFAP (blue, astrocyte antibody, 40× confocal imaging), or C) RABV P antigen (purple) and DAPI nuclear stain (blue, 63× confocal imaging). White arrows in (C) indicate regions positive for RABV P.

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - EGFP+ neurons are positive for RABV antigen.Brains were collected from Cre reporter mice fifteen days post-infection, cryosectioned, & EGFP+ regions compared to cell-specific labeling, A) NeuN (blue, neuronal nuclei antibody, 20× fluorescence imaging), B) GFAP (blue, astrocyte antibody, 40× confocal imaging), or C) RABV P antigen (purple) & DAPI nuclear stain (blue, 63× confocal imaging). White arrows in (C) indicate regions positive for RABV P. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.ppat.1002971), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GFAP Antibody [NB300-141] - Effects of the repeated administration of NVP CXCR2 20 (NVP; 10 μg/5 μl; i.t.; 16 h & 1 h before CCI & then once a day for 7 days) on the protein levels of CXCR2, IBA1, GFAP, CXCL1, CXCL2, & CXCL3 proteins (A–I) in the spinal cord (A–F) & DRG (G–I) on the 7th day after CCI in rats. The data are presented as the mean fold changes relative to the control ± SEM (5–6 samples per group). Intergroup differences were analyzed using ANOVA with Bonferroni's multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001 indicate differences vs. naive rats. #p < 0.05, indicate differences between V-treated & NVP-treated rats. CCI, chronic constriction injury; N, naive; V, vehicle; NVP, NVP CXCR2 20. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31616413), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Increased astrocyte activation in IL-1 beta -stimulated two-week old offspring is reduced by maternal LB supplementation. Representative images of fluorescence microscopy of claudin-5+ (location of the brain capillaries, red), GFAP+ astrocyte (green), & DAPI (nuclei, blue). Three to five sections per mouse were examined & at least three mice were examined in each group. Stronger than control SPF GFAP staining was observed around the blood vessel (see arrow) after IL-1 beta insult. Maternal supplemented group (LB) with or without postnatal insult had GFAP levels similar to the control group indicating that LB supplementation prevented astrocyte activation around the BBB endothelium. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32424168), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GFAP Antibody [NB300-141] - Glial cells density is differently modulated during AD progression.a Retinal slices were immunolabeled with anti-GFAP antibody (green) & Hoechst for nuclei visualization (blue) at different ages of 3xTg-AD & non-Tg mice & density of GFAP signal was quantified as shown in b (**p < 0.01 pre vs early; n = 16 fields/four slices for each condition; two-way ANOVA, Holm-Sidak; ##p < 0.01 for comparison with age-matched non-Tg mice, two-way ANOVA, Holm-Sidak). c Representative multiarea image of retinal slice immunolabeled with anti-Iba1 antibody (green) & Hoechst for nuclei visualization (blue); density of Iba1+ cells was quantified as shown in d (**p < 0.01 vs 3xTg-AD pre; ##p < 0.01 vs age-matched non-Tg mice, n = 16 fields/four slices for each condition, two-way ANOVA, Holm-Sidak method for multiple comparison) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29880901), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Eq, Gp, Rb
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

GFAP Antibody Summary

• Immunogen: This GFAP Antibody was developed against recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
• Marker: Astrocyte Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GFAP
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Simple Western 1:10000
  • Western Blot 1:5000
• Application Notes: In WB a band can be seen at 50-55 kDa representing GFAP. A lower band may be seen around 45 kDa representing a proteolytic fragment derived from the GFAP molecule. GFAP antibody validated for IHC-P from a verified customer review. IHC-Fr has been reported in scientific literature (PMID: 28040732).
  
  See Simple Western Antibody Database for Simple Western validation: tested in human brain lysate (0.05 mg/ml); separated by size, antibody dilution of 1:10,000; detects a band at 50 kDa; matrix was 12-230 kDa.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  GFAP Overexpression Lysate

Reactivity Notes

Predicted to work with most mammals. Chicken reactivity reported in scientific literature (PMID: 20844134). Rabbit and Guinea Pig reactivity reported in scientific literature (PMID: 4559710).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Supplied as serum
• Preservative: 5mM Sodium Azide
• Purity: Unpurified

Alternate Names for GFAP Antibody

• ALXDRD
• FLJ45472
• GFAP astrocytes
• GFAP immunohistochemistry
• GFAP mouse
• GFAP rabbit
• GFAP stain
• GFAP
• glial fibrillary acidic protein

Background

Glial fibrillary acidic protein (GFAP) is a class III intermediate filament protein that is largely expressed in astrocytes in addition to non-myelinating Schwann cells and glial cells (1,2). Other members of the type III intermediate filament family include desmin, peripherin, and vimentin (2-4). GFAP was first identified in the brains of multiple sclerosis patients (2). Human GFAP protein is 432 amino acids in length with a theoretical molecular weight of ~50 kDa (1,3). GFAP has at least 10 known isoforms, with the most prevalent and common in the brain being GFAP-alpha which is made of a head domain, a rod domain with four coils (1A, 1B, 2A, 2B) joined by linker regions, and a tail domain (1). GFAP is a marker of mature astrocytes, but is also expressed throughout development in both fetal and adult neural stem cells (2). While the exact function of GFAP is still elusive, it has been shown to play a role in cellular processes such as migration, mitosis, structural integrity, and signaling (2).

An increase in GFAP levels is often associated with neuroinflammation which results in the activation and proliferation of astroglia cell population (1,2). GFAP expression is also observed in brains of patients with neurodegenerative diseases including Alzheimer's and Parkinson's, epilepsy disorders, and brain injuries (1-4). Lesion sites associated with neurodegeneration can exhibit an array of gliosis characteristics from glial scarring with reduced astrocyte proliferation to activated, GFAP-positive astrocytes surrounding amyloid plaques (2). Furthermore, the GFAP gene is a target of single nucleotide polymorphisms in the coding region, considered a gain-of-function mutation, characterized by astrocytic inclusions, termed Rosenthal fibers, resulting in Alexander Disease (1-4). GFAP is also a center of many post-translational modifications, such as phosphorylation, which can alter various aspects of filament assembly (1,4).

References

1. Yang, Z., & Wang, K. K. (2015). Glial fibrillary acidic protein: from intermediate filament assembly and gliosis to neurobiomarker. Trends in Neurosciences. https://doi.org/10.1016/j.tins.2015.04.003

2. Hol, E. M., & Capetanaki, Y. (2017). Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin. Cold Spring Harbor Perspectives in Biology. https://doi.org/10.1101/cshperspect.a021642

3. Potokar, M., Morita, M., Wiche, G., & Jorgacevski, J. (2020). The Diversity of Intermediate Filaments in Astrocytes. Cells. https://doi.org/10.3390/cells9071604

4. Viedma-Poyatos, a., Pajares, M. A., & Perez-Sala, D. (2020). Type III intermediate filaments as targets and effectors of electrophiles and oxidants. Redox Biology. https://doi.org/10.1016/j.redox.2020.101582

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GFAP Antibody (NB300-141)(116)

Publications using NB300-141

• chen p, Lin M, Chen R et al. NeuroD1 improved neurocognitive function in a mouse model of subarachnoid hemorrhage Research Square 2023-04-03 (IHC, Mouse)
• Tiwari, A;Myeong, J;Hashemiaghdam, A;Stunault, MI;Zhang, H;Niu, X;Laramie, MA;Sponagel, J;Shriver, LP;Patti, GJ;Klyachko, VA;Ashrafi, G; Mitochondrial pyruvate transport regulates presynaptic metabolism and neurotransmission Science advances 2024-11-15 [PMID: 39546604]
• Hideki Nonaka, Takayuki Kondo, Mika Suga, Ryu Yamanaka, Yukako Sagara, Kayoko Tsukita, Naoko Mitsutomi, Kengo Homma, Ryuta Saito, Fumihiko Miyoshi, Hiromitsu Ohzeki, Masahiro Okuyama, Haruhisa Inoue Induced pluripotent stem cell‐based assays recapture multiple properties of human astrocytes Journal of Cellular and Molecular Medicine 2024-03-20 [PMID: 38509731]
• Karine Dos Santos Evangelho, Carlos Cifuentes-González, William Rojas-Carabali, Clemencia De Vivero-Arciniegas, Mariana Cañas-Arboleda, Gustavo Salguero, Carolina Ramírez-Santana, Alejandra de-la-Torre Mesenchymal stromal cells from human Wharton's jelly modulate the intraocular immune response in a glucocorticoid hypertension model: an exploratory analysis. Ophthalmic research 2024-03-06 [PMID: 38447539]
• Patrizia Ratano, Germana Cocozza, Cecilia Pinchera, Ludovica Maria Busdraghi, Iva Cantando, Katiuscia Martinello, Mariarosaria Scioli, Maria Rosito, Paola Bezzi, Sergio Fucile, Heike Wulff, Cristina Limatola, Giuseppina D’Alessandro Reduction of inflammation and mitochondrial degeneration in mutant SOD1 mice through inhibition of voltage-gated potassium channel Kv1.3 Frontiers in Molecular Neuroscience 2024-01-16 [PMID: 38292023]
• Chen P, Liu XY, Lin MH et Al. NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage J Neuroinflammation 2023-11-12 [PMID: 37953259]
• Chen Y, Wu XL, Hu HB et al. Neuronal MeCP2 in the dentate gyrus regulates mossy fiber sprouting of mice with temporal lobe epilepsy Neurobiology of disease 2023-11-01 [PMID: 37931884] (IHC, Mouse)
  
  Details:
  Dilution 1:2000
• Tan R, Hu X, Wang X et al. Leptin Promotes the Proliferation and Neuronal Differentiation of Neural Stem Cells through the Cooperative Action of MAPK/ERK1/2, JAK2/STAT3 and PI3K/AKT Signaling Pathways International journal of molecular sciences 2023-10-13 [PMID: 37894835] (ICC/IF, Rat)
• Richards T, Perron JC, Patel K et al. Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes Research square 2023-10-18 [PMID: 37886595] (IHC, Rat)
  
  Details:
  ICC/IF dilution 1:200; IHC dilution 1:500
• Williams EP, Xue Y, Lee J et al. Deep spatial profiling of Venezuelan equine encephalitis virus reveals increased genetic diversity amidst neuroinflammation and cell death during brain infection Journal of Virology 2023-08-31 [PMID: 37560924] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Wang C, Terrigno M, Li J et al. Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation Neuron 2023-06-23 [PMID: 37385246]
• Pawlik K, Ciapa?a K, Ciechanowska A et al. Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain Cells 2022-12-26 [PMID: 36611891] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Chang CY, Hung JH, Wu CC et al. Enhancer regulation for induced WNT3A expression during neuronal regeneration bioRxiv 2019-12-02 (Chemotaxis, Rat)
• Liao Z, Wang W, Deng W et al. Human Umbilical Cord Mesenchymal Stem Cells-Secreted TSG-6 Is Anti-Inflammatory and Promote Tissue Repair After Spinal Cord Injury ASN Neuro 2021-01-01 (IF/IHC, Rat)
• Grigorash BB, van Essen D, Liang G et al. p16High senescence restricts cellular plasticity during somatic cell reprogramming Nature cell biology 2023-09-01 [PMID: 37652981] (IHC-P, Mouse)
  
  Details:
  1:1000 IHC-P dilution
• Yang P, Liao C, Hu Q et al. Benzene, 1,2,4-Trimethoxy-5-(2-Methyl-1-Propen-1-yl) Attenuates D-galactose/AlCl3-induced Cognitive Impairment by Inhibiting Inflammation, Apoptosis, and improving Expression of Memory-Related Proteins Neuroscience 2023-06-14 [PMID: 37327966]
• Schneider KM, Blank N, Alvarez Y, Thum K et Al. The enteric nervous system relays psychological stress to intestinal inflammation Cell 2023-05-26 [PMID: 37236193]
• Ciechanowska A, Rojewska E, Piotrowska A et al. New insights into the analgesic properties of the XCL1/XCR1 and XCL1/ITGA9 axes modulation under neuropathic pain conditions - evidence from animal studies Frontiers in immunology 2022-12-22 [PMID: 36618360] (WB, Mouse)
• Richards K, Coulthard L, Rome C et al. Glass-brain mapping provides an adjunct tool for structural analysis in mouse models of neurodevelopmental disease Neuroimage: Reports 2021-06-01
• Poelaert KCK, van Kleef RGDM, Liu M et al. Enterovirus D-68 Infection of Primary Rat Cortical Neurons: Entry, Replication, and Functional Consequences mBio 2023-03-06 [PMID: 36877033] (Immunocytochemistry/ Immunofluorescence, Rat)
• Mona K, Marnick C, Sumin C et al. Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients Neuron 2022-11-01 [PMID: 36446381] (IF/IHC, Human)
• de Paiva I, Silva R, Mendonça I et al. Semaglutide Attenuates Anxious And Depressive-Like Behaviors and Reverses The Cognitive Impairment in a Type 2 Diabetes Mellitus Via The Microbiota-Gut-Brain Axis Research Square 2023-09-15 (IHC, Mouse)
• Kong W, Montano M, Corley MJ et al. Neuropilin-1 Mediates SARS-CoV-2 Infection of Astrocytes in Brain Organoids, Inducing Inflammation Leading to Dysfunction and Death of Neurons mBio 2022-10-31 [PMID: 36314791] (IHC-Fr, Human)
• Schr�ter M, Wang C, Terrigno M et al. Functional imaging of brain organoids using high-density microelectrode arrays MRS Bulletin 2022-06-30 [PMID: 36120104] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Kanaya A, Yang M, Emala C, Mikami M. Chronic allergic lung inflammation negatively influences neurobehavioral outcomes in mice Journal of Neuroinflammation 2022-08-31 [PMID: 36045388] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Gong J, Liu Y, Chung TH et al. Intracerebral lentiviral ABCD1 gene therapy in an early disease onset ALD mouse model Gene therapy 2022-07-06 [PMID: 35790794]
• Benton KC, Wheeler DS, Kurtoglu B et al. Norepinephrine activates ?(1) -adrenergic receptors at the inner nuclear membrane in astrocytes Glia 2022-09-01 [PMID: 35589612] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Killoy, KM;Harlan, BA;Pehar, M;Vargas, MR; NR1D1 downregulation in astrocytes induces a phenotype that is detrimental to cocultured motor neurons FASEB journal : official publication of the Federation of American Societies for Experimental Biology [PMID: 35319791] (ICC/IF, Mouse)
• Barut J, Rafa-Zablocka K, Jurga AM et al. Genetic lesions of the noradrenergic system trigger induction of oxidative stress and inflammation in the ventral midbrain Neurochemistry international 2022-02-09 [PMID: 35150790] (WB, Mouse)
• MendonCa IP, Paiva IHR, Duarte-Silva EP et al. Metformin and fluoxetine improve depressive-like behavior in a murine model of Parkinsons disease through the modulation of neuroinflammation, neurogenesis and neuroplasticity International immunopharmacology 2022-01-01 [PMID: 34890997] (IF/IHC, Mouse)
• Jin L, Xiao L, Ding M Et al. Heterogeneous macrophages contribute to the pathology of disc herniation induced radiculopathy The spine journal : official journal of the North American Spine Society 2021-10-27 [PMID: 34718176] (IF/IHC, Human)
• Kirschner A, Strat AN, Yablonski J Et al. Mechanosensitive channel inhibition attenuates TGF beta 2-induced actin cytoskeletal remodeling and reactivity in mouse optic nerve head astrocytes Exp Eye Res 2021-10-17 [PMID: 34656548] (IF/IHC, WB, Rat, Mouse)
• Wang Z, Wang H, Becker R et al. Acoustofluidic separation enables early diagnosis of traumatic brain injury based on circulating exosomes Microsyst Nanoeng 2021-09-27 [PMID: 34567734]
• Kilic U, Elibol B, Beker M et al. Inflammatory Cytokines are in Action: Brain Plasticity and Recovery after Brain Ischemia Due to Delayed Melatonin Administration Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 2021-09-18 [PMID: 34547676] (IF/IHC, Mouse)
• Jeon H, Kim M, Park W et al. Upregulation of AQP4 Improves Blood-Brain Barrier Integrity and Perihematomal Edema Following Intracerebral Hemorrhage Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 2021-09-20 [PMID: 34545550] (IF/IHC, Mouse)
• Serpe C, Monaco L, Relucenti M Et al. Microglia-Derived Small Extracellular Vesicles Reduce Glioma Growth by Modifying Tumor Cell Metabolism and Enhancing Glutamate Clearance through miR-124 Cells 2021-08-12 [PMID: 34440835] (IF/IHC, Mouse)
• Parvin S, Williams CR, Jarrett SA, Garraway SM Spinal Cord Injury Increases Pro-inflammatory Cytokine Expression in Kidney at Acute and Sub-chronic Stages Inflammation 2021-08-21 [PMID: 34417952] (WB, Mouse)
• Cocozza G, Garofalo S, Morotti M Et al. The feeding behaviour of ALS mouse models is modulated by the Ca2+ -activated KCa3.1 channels British journal of pharmacology 2021-08-19 [PMID: 34411281] (Mouse)
• Di Martino R, Sisalli MJ, Sirabella R et al. Ncx3-Induced Mitochondrial Dysfunction in Midbrain Leads to Neuroinflammation in Striatum of A53t-?-Synuclein Transgenic Old Mice International Journal of Molecular Sciences 2021-07-30 [PMID: 34360942] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Teng Y, Liu Z, Chen X et al. Conditional deficiency of m6A methyltransferase Mettl14 in substantia nigra alters dopaminergic neuron function Journal of Cellular and Molecular Medicine 2021-09-01 [PMID: 34288397] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Oliveira WH, Braga CF, LOs DB et al. Metformin prevents p-tau and amyloid plaque deposition and memory impairment in diabetic mice Experimental brain research 2021-07-20 [PMID: 34283253]
• Wang M, Feng L, Li Z et al. Thymosin beta-4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-kappa B signaling axis in APP/PS1 mice Journal of Neuroinflammation 2021-12-01 [PMID: 34183019] (IF/IHC, Mouse)
• Maimon R, Chillon-Marinas C, Snethlage CE et al. Therapeutically viable generation of neurons with antisense oligonucleotide suppression of PTB Nature neuroscience 2021-06-03 [PMID: 34083786] (IF/IHC, Mouse)
• Vegh C, Wear D, Okaj I et al. Combined Ubisol-Q10 and Ashwagandha Root Extract Target Multiple Biochemical Mechanisms and Reduces Neurodegeneration in a Paraquat-Induced Rat Model of Parkinsons Disease Antioxidants 2021-04-06 [PMID: 33917328] (IF/IHC, Rat)
• Sun Z, Cheng Z, Gong N et al. Neural presbycusis at ultra-high frequency in aged common marmosets and rhesus monkeys Aging (Albany NY) 2021-05-15 [PMID: 33909598] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Presa M, Bailey RM, Davis C et al. AAV9-mediated FIG4 delivery prolongs life span in Charcot Marie Tooth disease type 4J mouse model The Journal of clinical investigation 2021-04-20 [PMID: 33878035] (WB, Mouse)
• Derbis M, Kul E, Niewiadomska D et al. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats Nature communications 2021-02-24 [PMID: 33627639] (WB, Human)
• Lee H, Koh JY Roles for H+ /K+ -ATPase and zinc transportor 3 in cAMP-mediated lysosomal acidification in bafilomycin A1-treated astrocytes Glia 2020-12-14 [PMID: 33314298]
• Kikuoka R, Miyazaki I, Kubota N et al. Mirtazapine exerts astrocyte-mediated dopaminergic neuroprotection Sci Rep 2020-11-26 [PMID: 33244123] (IF/IHC, Rat)
  
  Details:
  Immunohistochemical analysis of rat neurons and astrocytes.
• Rimola, V, Hahnefeld, L Et al. Lysophospholipids Contribute to Oxaliplatin-Induced Acute Peripheral Pain. J Neurosci 2020-12-02 [PMID: 33158961] (WB, ICC/IF, Human)
• Zeltser N, Meyer I, Hernandez GV et al. Neurodegeneration in juvenile Iberian pigs with diet-induced nonalcoholic fatty liver disease Am. J. Physiol. Endocrinol. Metab. 2020-09-01 [PMID: 32744096]
• Zajaczkowska R, Kwiatkowski K, Pawlik K et al. Metamizole relieves pain by influencing cytokine levels in dorsal root ganglia in a rat model of neuropathic pain Pharmacol Rep 2020-07-20 [PMID: 32691345] (WB, Rat)
• Hernandez-Rodriguez M, Arciniega-Martinez IM, Garcia-Marin ID et al. Chronic Administration of Scopolamine Increased GSK3betaP9, Beta Secretase, Amyloid Beta, and Oxidative Stress in the Hippocampus of Wistar Rats Mol. Neurobiol. 2020-07-07 [PMID: 32638218]
  
  Details:
  Citation using the HRP format of this antibody.
• Foright RM, Johnson GC, Kahn D et Al. Compensatory eating behaviors in male and female rats in response to exercise training Am J Physiol Regul Integr Comp Physiol 2021-08-01 [PMID: 32551825]
• Lavi E, Cong L Type I Astrocytes and Microglia Induce a Cytokine Response in an Encephalitic Murine Coronavirus Infection Exp Mol Pathol. 2020-05-23 [PMID: 32454103] (ICC/IF, Mouse)
• Wang M, Zong Hf, Chang Kw et Al. 5-HT1AR alleviates A beta-induced cognitive decline and neuroinflammation through crosstalk with NF-kappa B pathway in mice Int. Immunopharmacol. 2020-01-01 [PMID: 32143008] (IF/IHC, Mouse)
• Chang CY, Liang MZ, Wu CC et al. WNT3A Promotes Neuronal Regeneration upon Traumatic Brain Injury Int J Mol Sci 2020-02-21 [PMID: 32098078] (IHC-P, Rat)
• Ciechanowska A, Popiolek-Barczyk K, Pawlik K et al. Changes in macrophage inflammatory protein-1 (MIP-1) family members expression induced by traumatic brain injury in mice Immunobiology 2020-02-01 [PMID: 32059938] (WB, Mouse)
• Cory M. Willis, Alexandra M. Nicaise, Ernesto R. Bongarzone, Maria Givogri, Cory R. Reiter, Olivia Heintz, Evan R. Jellison, Pearl A. Sutter, Gregg TeHennepe, Guruprasad Ananda, Anthony T. Vella, Stephen J. Crocker Astrocyte Support for Oligodendrocyte Differentiation can be Conveyed via Extracellular Vesicles but Diminishes with Age Scientific Reports 2020-01-21 [PMID: 31964978]
• Long-term culturing of porcine nodose ganglia Kuan SP, Atanasova KR, Guevara MV J Neurosci Methods [PMID: 31821820] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Tanaka T, Murata H, Miyazaki R, et al. Human recombinant erythropoietin improves motor function in rats with spinal cord compression-induced cervical myelopathy PLoS ONE 2019-12-10 [PMID: 31821342] (IF/IHC, Rat)
• Murtaj V, Belloli S, Di Grigoli G et al. Age and Sex Influence the Neuro-inflammatory Response to a Peripheral Acute LPS Challenge Front Aging Neurosci 2019-11-05 [PMID: 31749696] (IHC-Fr, Mouse)
• Mazur C, Powers B, Zasadny K et al. Brain pharmacology of intrathecal antisense oligonucleotides revealed through multimodal imaging JCI Insight 2019-10-17 [PMID: 31619586] (IHC-Fr, Rat)
• Piotrowska A, Rojewska E, Pawlik K et al. Pharmacological Blockade of Spinal CXCL3/CXCR2 Signaling by NVP CXCR2 20, a Selective CXCR2 Antagonist, Reduces Neuropathic Pain Following Peripheral Nerve Injury Front Immunol 2019-09-26 [PMID: 31616413] (WB, Mouse, Rat)
• McAvoy KM, Rajamohamed Sait H, Marsh G et al. Cell-autonomous and non-cell autonomous effects of neuronal BIN1 loss in vivo PLoS ONE 2019-08-13 [PMID: 31408457] (ICC/IF, Mouse, Rat)
• de Carvalho T, Peixoto C, de Paiva I et al. Does physical exercise influence in the development of Neuroeschistosomiasis? Brain Research Bulletin 2019-08-01 [PMID: 31377443] (IF/IHC, Mouse)
• FranCa MER, Ramos RKLG, Oliveira WH et al. Tadalafil restores long-term memory and synaptic plasticity in mice with hepatic encephalopathy Toxicol. Appl. Pharmacol. 2019-07-16 [PMID: 31323263] (IHC-P, Mouse)
• Shinozaki Y, Danjo Y, Koizumi S., et Al. Microglial ROCK is essential for chronic methylmercury-induced neurodegeneration J Neurochem 2019-07-07 [PMID: 31278875]
• Crotti A, Sait HR, McAvoy KM et al. BIN1 favors the spreading of Tau via extracellular vesicles Sci Rep 2019-07-01 [PMID: 31263146] (IF/IHC, Mouse)
• Schober ME, Requena DF, Casper TC Et al. Docosahexaenoic acid decreased neuroinflammation in rat pups after controlled cortical impact Exp Neurol 2019-06-28 [PMID: 31247195] (WB, Human)
• Epperly M W, Fisher R et al. Amelioration of Amyotrophic Lateral Sclerosis in SOD1G93A Mice by M2 Microglia from Transplanted Marrow. In Vivo 2019-04-28 [PMID: 31028184] (ICC/IF, Mouse)
• Gimenes AD, Andrade BFD, Pinotti JVP et al. Annexin A1-derived peptide Ac2-26 in a pilocarpine-induced status epilepticus model: anti-inflammatory and neuroprotective effects J Neuroinflammation. 2019-02-11 [PMID: 30755225] (WB, Rat)
• Garofalo S, Grimaldi A, Chece G et al. The glycoside oleandrin reduces glioma growth with direct and indirect effects on tumor cells. J. Neurosci. 2017-03-14 [PMID: 28292827] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Fil D, DeLoach A, Yadav S et al. Mutant Profilin1 transgenic mice recapitulate cardinal features of motor neuron disease Hum. Mol. Genet 2016-12-30 [PMID: 28040732] (IHC-Fr, Human)
• Pilat D, Piotrowska A, Rojewska E et al. Blockade of IL-18 signaling diminished neuropathic pain and enhanced the efficacy of morphine and buprenorphine. Mol. Cell. Neurosci. 2016-01-05 [PMID: 26763728] (WB, Rat)
• Chen P, Liu X, Lin M et al. NeuroD1 administration ameliorated neuroinflammation and boosted neurogenesis in a mouse model of subarachnoid hemorrhage Research Square 2023-06-30 (IHC-Fr, Mouse)
• Sankar SB, Pybus AF, Liew A et al. Low cerebral blood flow is a non-invasive biomarker of neuroinflammation after repetitive mild traumatic brain injury Neurobiol. Dis. 2018-12-25 [PMID: 30592976] (WB, Mouse)
• Lepore F, D'Alessandro G, Antonangeli F et al. CXCL16/CXCR6 Axis Drives Microglia/Macrophages Phenotype in Physiological Conditions and Plays a Crucial Role in Glioma Front Immunol 2018-11-27 [PMID: 30542347] (IF/IHC, Mouse)
• Cocozza G, di Castro MA, Carbonari L et al. Ca2+-activated k+ channels modulate microglia affecting motor neuron survival in hSOD1G93A mice. Brain Behav. Immun. 2018-07-03 [PMID: 29981425] (ICC/IF, Mouse)
• Grimaldi A, Brighi C, Peruzzi G et al. Inflammation, neurodegeneration and protein aggregation in the retina as ocular biomarkers for Alzheimer's disease in the 3xTg-AD mouse model Cell Death Dis 2018-06-07 [PMID: 29880901] (ICC/IF, Mouse)
• Grimaldi A, D'Alessandro G, Di Castro MA et al. Kv1.3 activity perturbs the homeostatic properties of astrocytes in glioma Sci Rep. 2018-05-15 [PMID: 29769580] (IF/IHC, Mouse)
• Jurga AM, Piotrowska A, Makuch W et al. Blockade of P2X4 Receptors Inhibits Neuropathic Pain-Related Behavior by Preventing MMP-9 Activation and, Consequently, Pronociceptive Interleukin Release in a Rat Model. Front Pharmacol. 2017-03-09 [PMID: 28275350] (WB, Rat)
• Klemis V, Ghura H et al. Circulating fibronectin contributes to mesangial expansion in a murine model of type 1 diabetes. Kidney Int 2017-01-06 [PMID: 28159318] (IF/IHC, Mouse)
• Heller JP, Michaluk P, Sugao K, Rusakov DA. Probing nano-organization of astroglia with multi-color super-resolution microscopy. J. Neurosci. Res. 2017-02-02 [PMID: 28151556]
• Zychowska M, Rojewska E, Makuch W et al. Participation of pro- and anti-nociceptive interleukins in botulinum toxin A-induced analgesia in a rat model of neuropathic pain. Eur. J. Pharmacol. 2016-09-09 [PMID: 27619001]
• Zhang B, Bailey WM, McVicar AL, Gensel JC. Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiol Aging 2016-11-01 [PMID: 27596335] (Immunocytochemistry/ Immunofluorescence, Guinea Pig, Human, Rabbit, Rat)
• Kenig S, Faoro V, Bourkoula E et al. Topoisomerase IIbeta mediates the resistance of glioblastoma stem cells to replication stress-inducing drugs Cancer Cell Int. 2016-07-27 [PMID: 27462186] (WB)
• Piotrowska A, Kwiatkowski K, Rojewska E. Direct and indirect pharmacological modulation of CCL2/CCR2 pathway results in attenuation of neuropathic pain-In vivo and in vitro evidence. J Neuroimmunol. 2016-08-15 [PMID: 27397071] (WB, Rat)
• Anzilotti S, Tornincasa M, Gerlini R et al. Genetic ablation of homeodomain-interacting protein kinase 2 selectively induces apoptosis of cerebellar Purkinje cells during adulthood and generates an ataxic-like phenotype. Cell Death Dis. 2015-12-04 [PMID: 26633710] (IHC-Fr, WB, Mouse)
• Kwon JJ, Nabinger SC, Vega Z et al. Pathophysiological role of microRNA-29 in pancreatic cancer stroma. Sci Rep 2015-01-01 [PMID: 26095125] (ICC/IF)
• Popiolek-Barczyk K, Kolosowska N. Parthenolide Relieves Pain and Promotes M2 Microglia/Macrophage Polarization in Rat Model of Neuropathy. Neural Plasticity. 2015-01-01 [PMID: 26090236] (WB, Rat)
• Northcott PA, Lee C, Zichner T et al. Enhancer hijacking activates GFI1 family oncogenes in medulloblastoma. Nature 2014-07-24 [PMID: 25043047]
• Ghods AJ, Glick R, Braun D, Feinstein D. Beneficial actions of the anti-inflammatory dimethyl fumarate in glioblastomas. Surg Neurol Int 2013-12-24 [PMID: 24404403] (ICC/IF, Mouse, Human)
• Adhi M, Cashman SM, Kumar-Singh R. Adeno-Associated Virus Mediated Delivery of a Non-Membrane Targeted Human Soluble CD59 Attenuates Some Aspects of Diabetic Retinopathy in Mice. PLoS One. 2013-10-22 [PMID: 24167638] (IF/IHC, ICC/IF, Mouse)
• D'Alessandro G, Catalano M, Sciaccaluga M et al. KCa3.1 channels are involved in the infiltrative behavior of glioblastoma in vivo. Cell Death Dis. 2013-08-15 [PMID: 23949222] (ICC/IF, Human, Mouse)
• Xia G, Santostefano KE, Goodwin M et al. Generation of Neural Cells from DM1 Induced Pluripotent Stem Cells As Cellular Model for the Study of Central Nervous System Neuropathogenesis Cell Reprogram 2013-04-01 [PMID: 23550732]
• Xia G, Santostefano K, Hamazaki T et al. Generation of Human-Induced Pluripotent Stem Cells to Model Spinocerebellar Ataxia Type 2 In vitro J Mol Neurosci 2012-12-09 [PMID: 23224816] (ICC/IF, Human)
• Gomme EA, Wirblich C, Addya S et al. Immune Clearance of Attenuated Rabies Virus Results in Neuronal Survival with Altered Gene Expression PLoS Pathog 2012-10-01 [PMID: 23071441] (ICC/IF, IHC-Fr, Mouse)
• Gatzioufas Z, Stupp T, Moschos MM et al. Effect of botulinum toxin A on the intraocular pressure and the retina in an animal model Cutan Ocul Toxicol 2012-08-24 [PMID: 22917001] (ICC/IF, IF/IHC, Rat)
• O'Donnell LA, Conway S, Rose RW, Nicolas E, Slifker M, Balachandran S, Rall GF. STAT1-Independent Control of a Neurotropic Measles Virus Challenge in Primary Neurons and Infected Mice. J Immunol;188(4):1915-23. 2012-02-15 [PMID: 22246627] (ICC/IF, Mouse)
• Sims B, Clarke M, Francillion L et al. Hypoxic preconditioning involves system Xc(-) regulation in mouse neural stem cells. Stem Cell Research. 2011-09-28 [PMID: 22056639] (ICC/IF, Mouse)
• Wu K, Li S, Bodhinathan K et al. Enhanced expression of Pctk1, Tcf12 and Ccnd1 in hippocampus of rats: Impact on cognitive function, synaptic plasticity and pathology. Neurobiology of Learning and Memory. 2011-09-25 [PMID: 21982980] (IHC-Fr, Rat)
• Traudt CM, Tkac I, Ennis KM, Sutton LM, Mammel DM, Rao R. Postnatal morphine administration alters hippocampal development in rats. J Neurosci Res;90(1):307-14. 2012-01-01 [PMID: 21971612] (ICC/IF, IF/IHC, Rat)
• Obara I, Tochiki KK, Geranton SM et al. Systemic inhibition of the mammalian target of rapamycin (mTOR) pathway reduces neuropathic pain in mice. Pain. 2011-09-12 [PMID: 21917376] (WB, Mouse)
• Alexanian AR, Fehlings MG, Zhang Z, Maiman DJ. Transplanted Neurally Modified Bone Marrow-Derived Mesenchymal Stem Cells Promote Tissue Protection and Locomotor Recovery in Spinal Cord Injured Rats. Neurorehabil Neural Repair. 2011-08-15 [PMID: 21844281] (ICC/IF, Rat)
• Poole DP, Pelayo JC, Cattaruzza F et al. Transient receptor potential ankyrin 1 is expressed by inhibitory motoneurons of the mouse intestine Gastroenterology 2011-08-01 [PMID: 21689654] (IF/IHC, ICC/IF, Mouse)
• Marroquin Belaunzaran O, Cordero M et al. Chronic delivery of antibody fragments using immunoisolated cell implants as a passive vaccination tool PLoS One 2011-04-20 [PMID: 21533130] (ICC/IF, IF/IHC, Mouse)
• Chen W-S, Yueh C-Y, Huang Y-A, Hwang E. An inverted method for culturing dissociated mouse hippocampal neurons. Neurosci Res. 2011-01-01 [PMID: 21241744] (ICC/IF, Mouse)
• Uthaiah RC, Hudspeth AJ. Molecular anatomy of the hair cell's ribbon synapse. J Neurosci 2010-09-15 [PMID: 20844134] (WB, Mouse, Chicken)
• Wu Q, Howell MP, Palmiter RD. Ablation of Neurons Expressing Agouti-Related Protein Activates Fos Gliosis in Postsynaptic Target Regions. J Neurosci;28(37):9218-9226. 2008-01-01 [PMID: 18784302] (IF/IHC, Mouse)
• Maezawa Izumi, Maeda Nobuyo, Montine Thomas J et al. Apolipoprotein E-specific innate immune response in astrocytes from targeted replacement mice. Journal of Neuroinflammation 2006-01-01 [PMID: 16603079] (WB, Mouse)
• Maezawa, I et al. Neurotoxicity from innate immune response is greatest with targeted replcament of e4 allele of apolipoprotein E gene is mediated by microglial p38MAPK. FASEB J. 2006-01-01 [PMID: 16481366] (IF/IHC, Mouse)
• Li Y, Fu L, Gonzales DM et al. Coronavirus neurovirulence correlates with the ability of the virus to induce proinflammatory cytokine signals from astrocytes and microglia. J Virol 2004-04-01 [PMID: 15016862] (ICC/IF, Mouse)
• Henion, T et al. Expression of dystroglycan, fukutin POMGnT1 during mouse cerebellar development. Molecular Brain Research 112:177-181. 2003-01-01 [PMID: 12670716] (ICC/IF, Mouse)
• Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 43:429-35. 1972-01-01 [PMID: 4559710] (ICC/IF, Rabbit, Rat, Guinea Pig, Human)

Reviews for GFAP Antibody (NB300-141) (12)

Average Rating: 4.7

(Based on 12 reviews)

We have 12 reviews tested in 6 species: Human, Mouse, Rat, Bovine, Goat, Pig.

reviewed by: Verified Customer WB Bovine 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: retina
• Species: Bovine
• Lot: 179040517

reviewed by: Verified Customer WB Goat 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: 30ug,retina
• Species: Goat
• Lot: 179040517

reviewed by: Verified Customer WB Pig 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: retina
• Species: Pig
• Lot: 179040517

Comments

• Comments: use at 1:500

reviewed by: Verified Customer WB Human 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: retina
• Species: Human
• Lot: 179040517

reviewed by: Verified Customer WB Mouse 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: retina
• Species: Mouse
• Lot: 179040517

reviewed by: Verified Customer WB Rat 07/28/2020

Summary

• Application: Western Blot
• Sample Tested: retina
• Species: Rat
• Lot: 179-040517

reviewed by: Verified Customer ICC Human 06/23/2020

Summary

• Application: Immunocytochemistry
• Sample Tested: mixed neuronal primary culture
• Species: Human
• Lot: 179040517

reviewed by: Verified Customer IHC-P Mouse 06/07/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse brain
• Species: Mouse
• Lot: 121712

Comments

• Comments: Proteinasi K treatment; Primary Ab 1:1000. Detection with Polymer-HRP.

reviewed by: Verified Customer IHC Mouse 06/29/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: PFA Fixed Frozen Section of Mouse Brain
• Species: Mouse

Comments

• Comments: WD [1:K]; Secondary ab RTU Polymer-HRP

reviewed by: Verified Customer ICC Mouse 04/18/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary mouse cortical astrocytes
• Species: Mouse

reviewed by: Janosch Heller ICC Rat 03/27/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary rat cortical neurons
• Species: Rat

Comments

• Comments: Fixation: 4% paraformaldehyde (TAAB) in PBS, 15 minutes RT
  Blocking: 10% donkey serum and 1% BSA in PBS with 0.1% saponin, 1 hour RT
  Permeabilization: 0.1% saponin
  Primary Antibody: 1:1000 in PBS with 0.1% saponin, overnight, 4°C
  Wash: three times wash with PBS with 0.1% saponin, 10 min each
  Secondary Antibody: Invitrogen goat anti-rabbit Alexa647, 1:500 in PBS with 0.1% saponin, 2 hours, RT
  Wash: two washes with PBS with 0.1% saponin, 10 min each and three washes with PBS, 10 min each
  Microscope: Burker Vutara 350
  
  The antibody labels intermediate filaments in cultured astrocytes.

reviewed by: Verified Customer IF Mouse 01/17/2014

Summary

• Application: Immunofluorescence
• Species: Mouse

FAQs for GFAP Antibody (NB300-141). (Showing 1 - 3 of 3 FAQs).

1. I am interested in native or recombinant GFAP protein without GST Tag or in urea buffer. Can I use your GFAP antibody (NB100-53809) to detect P0809, P0810 and P1691 GFAP proteins?
   • P0809, P0810 and P1691 are blocking peptides that are not suitable for use with NB100-53809. Unfortunately, we do not carry a GFAP protein without a GST tag. I apologize for the inconvenience.
2. Our customer would like to know if your antibody is suitable for frozen sample by IF.
   • NB300-141 has been tested and validated in IHC-Frozen and IF; this is covered by our guarantee.
3. I would like to use your GFAP-antibody (cat # NB300-141) in a Western Blot experiment. Could you tell me the dilution of this antibody?
   • The dilution recommended for Western blot using NB300-141 is 1:50,000.

Bioinformatics

• Gene Symbol: GFAP
• Entrez:
  • 2670(Human)
  • 14580(Mouse)
  • 24387(Rat)
• Uniprot:
  • P14136()
  • Q28115(Bovine)