Recombinant Human LIF Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human LIF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 ng/mL.
Source
E. coli-derived human LIF protein
Pro24-Phe202
Accession #
N-terminal Sequence
Pro24
Protein/Peptide Type
Recombinant Proteins
Gene
LIF
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19 kDa, reducing conditions
Publications
Read Publications using
7734-LF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIF Protein

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDA
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines (1‑4). The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively (5). LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences (5). Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases (3, 4, 6). Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6,
IL‑11, and IL‑27 (3, 6). A soluble LIFR has been reported in the mouse (7). Depending on the cells and their context, LIF either opposes or favors differentiation (3, 8). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (3, 6, 8). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (3, 6). LIF is produced by CD4+ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation (3, 4). LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination (3, 4, 9). LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone (10). LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1 (2, 11). Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages (12). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (3, 4). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system (3, 4, 6, 8).

  1. Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. USA 85:2623.
  2. Moreau, J.F. et al. (1988) Nature 336:690.
  3. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  4. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  5. Voyle, R.B. et al. (1999) Exp. Cell Res. 249:199.
  6. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  7. Tomida, M. et al. (1993) FEBS lett. 334:193.
  8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  9. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  10. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  11. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  12. Duluc, D. et al. (2007) Blood 110:4319.

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Publications for LIF (7734-LF)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(13)
Cell Culture
(2)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rat
(1)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 7734-LF Applications Species
F Fang, Z Li, Q Zhao, C Xiong, K Ni Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells Stem Cell Res Ther, 2020;11(1):100. 2020 [PMID: 32127035] (Cell Culture, Human) Cell Culture Human
Z Li, F Fang, Q Zhao, H Li, C Xiong Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells Stem Cell Res Ther, 2019;10(1):324. 2019 [PMID: 31730021] (Cell Culture, Human) Cell Culture Human
A Tscherner, AC Brown, L Stalker, J Kao, I Dufort, MA Sirard, J LaMarre STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation Sci Rep, 2018;8(1):11527. 2018 [PMID: 30068990] (Bioassay, Bovine) Bioassay Bovine
L Alabdi, M He, Q Yang, AB Norvil, H Gowher The transcription factor Vezf1 represses the expression of the antiangiogenic factor d2 in endothelial cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29794136] (Bioassay, Human) Bioassay Human
H Li, J Yao, X Chang, J Wu, T Duan, K Wang LIFR increases the release of sEng via the up-regulation of MMP14 expression in PE Reproduction, 2018;0(0):. 2018 [PMID: 29363569] (Bioassay, Human) Bioassay Human
S Jiao, Y Liu, Y Yao, J Teng miR-124 promotes proliferation and differentiation of neuronal stem cells through inactivating Notch pathway Cell Biosci, 2017;7(0):68. 2017 [PMID: 29238518] (Bioassay, Rat) Bioassay Rat
H Zhang, F Sun, J Wang, L Xie, C Yang, M Pan, B Shao, GY Yang, SH Yang, Q ZhuGe, K Jin Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke Aging Dis, 2017;8(2):203-214. 2017 [PMID: 28400986] (Bioassay, Human) Bioassay Human
Enrique Armijo Increased susceptibility to a? toxicity in neuronal cultures derived from familial Azheimer&#039;s disease (PSEN1-A246E) induced pluripotent stem cells Neurosci. Lett, 2016;0(0):. 2016 [PMID: 28034781] (Bioassay, Human) Bioassay Human
Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow Nat Cell Biol, 2016;18(10):1078-1089. 2016 [PMID: 27642788] (Bioassay, Human) Bioassay Human
Hong S, Choi J, Lee S, Park Y, Park K, Lee J, Kim J, Gajulapati V, Goo J, Singh S, Lee K, Kim Y, Im S, Ahn S, Rose-John S, Heo T, Choi Y A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor beta Subunit, Glycoprotein 130. J Immunol, 2015;195(1):237-45. 2015 [PMID: 26026064] (Bioassay, Human) Bioassay Human
Show All 15 Publications.

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Blogs on LIF.

Using a STAT3 antibody in chromatin immunoprecipitation (ChIP)
Signal transducer and activator of transcription 3 (STAT3) is an important oncogenic transcriptional factor that mediates tumor induced immune suppression.  Specifically, STAT3 transmits signals from cytokines and growth factor receptors in the pla...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

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Bioinformatics

Gene Symbol LIF
Uniprot