Recombinant Human LIF Protein


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human LIF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.12 ng/mL.
E. coli-derived human LIF protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19 kDa, reducing conditions
Read Publications using
7734-LF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIF Protein

  • CDF
  • D Factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor


LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines (1‑4). The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively (5). LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences (5). Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases (3, 4, 6). Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6,
IL‑11, and IL‑27 (3, 6). A soluble LIFR has been reported in the mouse (7). Depending on the cells and their context, LIF either opposes or favors differentiation (3, 8). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (3, 6, 8). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (3, 6). LIF is produced by CD4+ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation (3, 4). LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination (3, 4, 9). LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone (10). LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1 (2, 11). Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages (12). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (3, 4). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system (3, 4, 6, 8).

  1. Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. USA 85:2623.
  2. Moreau, J.F. et al. (1988) Nature 336:690.
  3. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51.
  4. Metcalfe, S.M. (2011) Genes Immun. 12:157.
  5. Voyle, R.B. et al. (1999) Exp. Cell Res. 249:199.
  6. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680.
  7. Tomida, M. et al. (1993) FEBS lett. 334:193.
  8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  9. Slaets, H. et al. (2010) Trends Mol. Med. 16:493.
  10. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145.
  11. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977.
  12. Duluc, D. et al. (2007) Blood 110:4319.

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Publications for LIF (7734-LF)(28)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 3 applications: Bioassay, Cell Culture, Surface Plasmon Resonance.

Filter By Application
Cell Culture
Surface Plasmon Resonance
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Filter By Species
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using 7734-LF Applications Species
Jovanovic, VM;Weber, C;Slamecka, J;Ryu, S;Chu, PH;Sen, C;Inman, J;De Sousa, JF;Barnaeva, E;Hirst, M;Galbraith, D;Ormanoglu, P;Jethmalani, Y;Mercado, JC;Michael, S;Ward, ME;Simeonov, A;Voss, TC;Tristan, CA;Singeç, I; A defined roadmap of radial glia and astrocyte differentiation from human pluripotent stem cells Stem cell reports 2023-07-04 [PMID: 37451260] (Bioassay, Human) Bioassay Human
J Li, Q Zhu, J Cao, Y Liu, Y Lu, Y Sun, Q Li, Y Huang, S Shang, X Bian, C Li, L Zhang, Y Wang, Y Nie, J Fu, W Li, MA Mazid, Y Jiang, W Jia, X Wang, Y Sun, MA Esteban, Q Sun, F Zhou, Z Liu Cynomolgus monkey embryo model captures gastrulation and early pregnancy Cell Stem Cell, 2023-04-06;30(4):362-377.e7. 2023-04-06 [PMID: 37028403] (Bioassay, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)) Bioassay Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
S Xu, X Yang, Q Chen, Z Liu, Y Chen, X Yao, A Xiao, J Tian, L Xie, M Zhou, Z Hu, F Zhu, X Xu, F Hou, J Nie Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis EBioMedicine, 2022-11-04;86(0):104312. 2022-11-04 [PMID: 36335669] (Bioassay, Mouse, Rat) Bioassay Mouse, Rat
Z Li, F Fang, Y Long, Q Zhao, X Wang, Z Ye, T Meng, X Gu, W Xiang, C Xiong, H Li The balance between NANOG and SOX17 mediated by TET proteins regulates specification of human primordial germ cell fate Cell & bioscience, 2022-11-04;12(1):181. 2022-11-04 [PMID: 36333732] (Bioassay, Human) Bioassay Human
SW Wang, MJ Herriges, K Hurley, DN Kotton, AM Klein CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information Nature Biotechnology, 2022-02-21;0(0):. 2022-02-21 [PMID: 35190690] (Bioassay, Human) Bioassay Human
M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 2022-01-27;23(3):. 2022-01-27 [PMID: 35163371] (Bioassay, Human) Bioassay Human
YC Wen, CL Liu, HL Yeh, WH Chen, KC Jiang, VTN Tram, M Hsiao, J Huang, WY Chen, YN Liu PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer British Journal of Cancer, 2021-11-24;0(0):. 2021-11-24 [PMID: 34815524] (Bioassay, Human) Bioassay Human
Y Fan, Z Min, S Alsolami, Z Ma, E Zhang, W Chen, K Zhong, W Pei, X Kang, P Zhang, Y Wang, Y Zhang, L Zhan, H Zhu, C An, R Li, J Qiao, T Tan, M Li, Y Yu Generation of human blastocyst-like structures from pluripotent stem cells Cell Discovery, 2021-09-07;7(1):81. 2021-09-07 [PMID: 34489415] (Bioassay, Human) Bioassay Human
H Wang, S Si, M Jiang, L Chen, K Huang, W Yu Leukemia inhibitory factor is involved in the pathogenesis of NSCLC through activation of the STAT3 signaling pathway Oncology Letters, 2021-07-14;22(3):663. 2021-07-14 [PMID: 34386085] (Bioassay, Human) Bioassay Human
ME Clements, L Holtslande, C Edwards, V Todd, SDR Dooyema, K Bullock, K Bergdorf, CA Zahnow, RM Connolly, RW Johnson HDAC inhibitors induce LIFR expression and promote a dormancy phenotype in breast cancer Oncogene, 2021-07-10;0(0):. 2021-07-10 [PMID: 34247191] (Bioassay, Human) Bioassay Human
Show All 28 Publications.

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Gene Symbol LIF