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KLF4 Antibody [Unconjugated]

Images

 
D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen ...read more
KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
KLF4 was detected in perfusion fixed frozen sections of mouse colon tissue using Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 1 µg/mL overnight at 4 °C. Before ...read more
Overexpression of beta -catenin maintains B6 ESC self-renewal in the presence of LIF.(A) Immunoblot analysis of phospho-beta -catenin, beta -catenin and beta -actin levels in B6 ES cells cultured in 1000 U/ml LIF ...read more
LIF maintains B6 ES cell self-renewal in the presence of CHIR99021.(A) Phase contrast image of B6 ES cells in the presence of 1000 U/ml LIF supplemented without or with 3 µM CHIR99021 (CH) for 4 days. Scale bars ...read more
Overlapping SIM2 occupancy with master transcription factor binding sites.a. Frequency distribution of OCT4, SOX2 and NANOG DNA binding sites in a 40kb window centered to the newly identified SIM2 DNA binding sites. ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

KLF4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse KLF4
Ala11-Phe483
Accession # Q60793
Specificity
Detects mouse KLF4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLF4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF3158 in the following applications:

Publications
Read Publications using
AF3158 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF4 Antibody [Unconjugated]

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Background

KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF4 Antibody (AF3158)(86)

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 8 applications: ChIP, Co-Immunoprecipitation, Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(3)
Co-Immunoprecipitation
(1)
Flow Cytometry
(1)
ICC
(13)
IHC
(15)
IHC-P
(2)
Immunoprecipitation
(4)
Western Blot
(15)
All Applications
Filter By Species
Human
(6)
Mouse
(42)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using AF3158 Applications Species
Sreeparna Chakraborty, Abhalaxmi Singh, Li Wang, Xinge Wang, Mark A. Sanborn, Zijing Ye et al. Trained immunity of alveolar macrophages enhances injury resolution via KLF4-MERTK-mediated efferocytosis Journal of Experimental Medicine 2023-11-06 [PMID: 37615937]
Kuheli Banerjee, Yanzhu Lin, Johannes Gahn, Julio Cordero, Purnima Gupta, Islam Mohamed et al. SMAD4 maintains the fluid shear stress set point to protect against arterial-venous malformations Journal of Clinical Investigation 2023-09-15 [PMID: 37490341]
Sato, S;Hishida, T;Kinouchi, K;Hatanaka, F;Li, Y;Nguyen, Q;Chen, Y;Wang, PH;Kessenbrock, K;Li, W;Izpisua Belmonte, JC;Sassone-Corsi, P; The circadian clock CRY1 regulates pluripotent stem cell identity and somatic cell reprogramming Cell reports 2023-05-31 [PMID: 37261952] (Western Blot, Human) Western Blot Human
S Brahma, S Henikoff RNA Polymerase II, the BAF remodeler and transcription factors synergize to evict nucleosomes bioRxiv : the preprint server for biology, 2023-01-22;0(0):. 2023-01-22 [PMID: 36711459] (ICC, Mouse) ICC Mouse
X Yang, ST Wu, R Gao, R Wang, Y Wang, Z Dong, L Wang, C Qi, X Wang, ML Schmitz, R Liu, Z Han, L Wang, X Zheng Release of STK24/25 suppression on MEKK3 signaling in endothelial cells confers Cerebral cavernous malformation JCI Insight, 2023-03-08;0(0):. 2023-03-08 [PMID: 36692953] (IHC, Mouse) IHC Mouse
H Maeda, H Yao, H Go, KE Huntington, ME De Paepe, PA Dennery Involvement of miRNA-34a regulated Kr�ppel-like factor 4 expression in hyperoxia-induced senescence in lung epithelial cells Respiratory Research, 2022-12-10;23(1):340. 12/10/2022 [PMID: 36496404]
J Bi, W Wang, M Zhang, B Zhang, M Liu, G Su, F Chen, B Chen, T Shi, Y Zheng, X Zhao, Z Zhao, J Shi, P Li, L Zhang, W Lu KLF4 inhibits early neural differentiation of ESCs by coordinating specific 3D chromatin structure Nucleic Acids Research, 2022-11-28;50(21):12235-12250. 2022-11-28 [PMID: 36477888] (Western Blot, Mouse) Western Blot Mouse
Zifeng Dai, Jingwei Li, Ying Li, Rui Wang, Huili Yan, Ziyu Xiong et al. Role of pericytes in the development of cerebral cavernous malformations iScience 2022-12-22 [PMID: 36465134]
S Yu, C Zhou, J He, Z Yao, X Huang, B Rong, H Zhu, S Wang, S Chen, X Wang, B Cai, G Zhao, Y Chen, L Xiao, H Liu, Y Qin, J Guo, H Wu, Z Zhang, M Zhang, X Zhao, F Lan, Y Wang, J Chen, S Cao, D Pei, J Liu BMP4 drives primed to na�ve transition through PGC-like state Nature Communications, 2022-05-19;13(1):2756. 2022-05-19 [PMID: 35589713] (ICC, Mouse) ICC Mouse
LY Guo, J Bian, AE Davis, P Liu, HR Kempton, X Zhang, A Chemparath, B Gu, X Lin, DA Rane, X Xu, RM Jamiolkows, Y Hu, S Wang, LS Qi Multiplexed genome regulation in vivo with hyper-efficient Cas12a Nature Cell Biology, 2022-04-12;24(4):590-600. 2022-04-12 [PMID: 35414015] (ICC, Mouse) ICC Mouse
Show All 86 Publications.

Reviews for KLF4 Antibody (AF3158) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF3158:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23395636
SpeciesMouse
  4
reviewed by:
Verified Customer
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20550931
SpeciesMouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KLF4 Antibody (AF3158). (Showing 1 - 1 of 1 FAQs).

  1. Can I use this antibody in rabbit samples?
    • AF3158 is against KLF4 and is derived from mouse. Unfortunately, it has not been tested with the rabbit samples. Therefore we don't know for sure whether this antibody will work in rabbit. Since it is a polyclonal antibody, the likelihood that this antibody will recognize the rabbit KLF4 is good.

Secondary Antibodies

 

Isotype Controls

Additional KLF4 Products

Blogs on KLF4.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Recent Reviews

4
5
0
4
2
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0
2
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1
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Verified Customer
01/07/2015
Application: WB
Species: Mouse

 
Verified Customer
01/07/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol KLF4
Uniprot