KLF4 Antibody [Unconjugated]

Images

 
D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. KLF4/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Mouse KLF4 Antigen ...read more
KLF4 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more
KLF4 was detected in perfusion fixed frozen sections of mouse colon tissue using Goat Anti-Mouse KLF4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3158) at 1 µg/mL overnight at 4 °C. Before ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

KLF4 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant mouse KLF4
Ala11-Phe483
Accession # Q60793
Specificity
Detects mouse KLF4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLF4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF3158 in the following applications:

Publications
Read Publications using
AF3158 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF4 Antibody [Unconjugated]

  • Epithelial zinc finger protein EZF
  • EZF
  • EZFendothelial Kruppel-like zinc finger protein
  • GKLFKrueppel-like factor 4
  • Gut-enriched krueppel-like factor
  • KLF4
  • Kruppel-like factor 4 (gut)

Background

KLF4 belongs to the KLF family of transcription factors that regulate several cell functions. It is highly expressed in differentiated epithelial cells of the gastrointestinal tract. Human and mouse KLF4 share 91% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KLF4 Antibody (AF3158)(40)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, ICC, IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(3)
ICC
(8)
IHC
(10)
IHC-P
(1)
Immunoprecipitation
(3)
Western Blot
(12)
All Applications
Filter By Species
Human
(6)
Mouse
(31)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using AF3158 Applications Species
J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs Nature Communications, 2021;12(1):7322. 2021 [PMID: 34916498] (ICC, Mouse) ICC Mouse
S Huang, P Kuri, Y Aubert, M Brewster, N Li, O Farrelly, G Rice, H Bae, S Prouty, T Dentchev, W Luo, BC Capell, P Rompolas Lgr6 marks epidermal stem cells with a nerve-dependent role in wound re-epithelialization Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34102139] (IHC, Mouse) IHC Mouse
J Bayerl, M Ayyash, T Shani, YS Manor, O Gafni, R Massarwa, Y Kalma, A Aguilera-C, M Zerbib, H Amir, D Sheban, S Geula, N Mor, L Weinberger, S Naveh Tass, V Krupalnik, B Oldak, N Livnat, S Tarazi, S Tawil, E Wildschutz, S Ashouokhi, L Lasman, V Rotter, S Hanna, D Ben-Yosef, N Novershter, S Viukov, JH Hanna Principles of signaling pathway modulation for enhancing human naive pluripotency induction Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33915080] (ChIP, Human) ChIP Human
A Sünderhauf, M Hicken, H Schlichtin, K Skibbe, M Ragab, A Raschdorf, M Hirose, H Schäffler, A Bokemeyer, D Bettenwort, AG Savitt, S Perner, S Ibrahim, EL Peerschke, B Ghebrehiwe, S Derer, C Sina Loss of mucosal p32/gC1qR/HABP1 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitis Cellular and Molecular Gastroenterology and Hepatology, 2021;0(0):. 2021 [PMID: 33515804] (Western Blot, Human) Western Blot Human
Y Lu, B Brommer, X Tian, A Krishnan, M Meer, C Wang, DL Vera, Q Zeng, D Yu, MS Bonkowski, JH Yang, S Zhou, EM Hoffmann, MM Karg, MB Schultz, AE Kane, N Davidsohn, E Korobkina, K Chwalek, LA Rajman, GM Church, K Hochedling, VN Gladyshev, S Horvath, ME Levine, MS Gregory-Ks, BR Ksander, Z He, DA Sinclair Reprogramming to recover youthful epigenetic information and restore vision Nature, 2020;588(7836):124-129. 2020 [PMID: 33268865] (IHC, Mouse) IHC Mouse
EJ Orzechowsk, T Katano, AB Bialkowska, VW Yang Interplay among p21Waf1/Cip1, MUSASHI-1 and Kr�ppel-like factor 4 in activation of Bmi1-CreER reserve intestinal stem cells after gamma radiation-induced injury Sci Rep, 2020;10(1):18300. 2020 [PMID: 33110120] (IHC, Mouse) IHC Mouse
Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging Cell Mol Gastroenterol Hepatol, 2020;0(0):. 2020 [PMID: 32771388] (Western Blot, Human) Western Blot Human
B He, A Johansson-, J Backhouse, J Li, GYF Lee, J Hamzah, R Ganss Remodeling of Metastatic Vasculature Reduces Lung Colonization and Sensitizes Overt Metastases to Immunotherapy Cell Rep, 2020;30(3):714-724.e5. 2020 [PMID: 31968248] (ICC, Mouse) ICC Mouse
R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes Nat Commun, 2019;10(1):2817. 2019 [PMID: 31249304] (IHC, Mouse) IHC Mouse
T Kalkan, S Bornelöv, C Mulas, E Diamanti, T Lohoff, M Ralser, S Middelkamp, P Lombard, J Nichols, A Smith Complementary Activity of ETV5, RBPJ, and TCF3 Drives Formative Transition from Naive Pluripotency Cell Stem Cell, 2019;0(0):. 2019 [PMID: 31031137] (ICC, Mouse) ICC Mouse
Show All 40 Publications.

Reviews for KLF4 Antibody (AF3158) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF3158:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23395636
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23395636

Details

Detection NotesSee PMID 23395636

Comments

CommentsPublished: See PMID 23395636Independent review from antibodyresource.com
  4
reviewed by:
Verified Customer
WB Mouse 01/07/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 20550931
SpeciesMouse

Blocking

Blocking DetailsSee PMID 20550931

Details

Detection NotesSee PMID 20550931

Comments

CommentsPublished: See PMID 20550931Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for KLF4 Antibody (AF3158). (Showing 1 - 1 of 1 FAQs).

  1. Can I use this antibody in rabbit samples?
    • AF3158 is against KLF4 and is derived from mouse. Unfortunately, it has not been tested with the rabbit samples. Therefore we don't know for sure whether this antibody will work in rabbit. Since it is a polyclonal antibody, the likelihood that this antibody will recognize the rabbit KLF4 is good.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for KLF4 Antibody (AF3158)

Discover related pathways, diseases and genes to KLF4 Antibody (AF3158). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for KLF4 Antibody (AF3158)

Discover more about diseases related to KLF4 Antibody (AF3158).
 

Pathways for KLF4 Antibody (AF3158)

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Blogs on KLF4.

Breast cancer stem cells survive chemotherapy through S100A10-ANXA2-SPT6 interaction that epigenetically promotes OCT4-mediated stemness
By Jamshed Arslan, Pharm D, PhDBreast cancer is the most common cancer among women that causes the greatest number of cancer-related deaths worldwide. After radiotherapy or cytotoxic chemotherapy like paclitax...  Read full blog post.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

KLF4 opens the door for stem cell research
KLF4 (Kruppel-like factor 4, Epithelial zinc finger protein EZF) is a zinc finger transcription factor thought to be involved in developmental differentiation and proliferation. It is considered a pluripotency reprogramming factor (PRF) due to its abi...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Verified Customer
01/07/2015
Application: WB
Species: Mouse

 
Verified Customer
01/07/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol KLF4
Uniprot