LIN-28A Antibody

Images

 
Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen ...read more
LIN‑28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. ...read more
LIN‑28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

LIN-28A Antibody Summary

Immunogen
E. coli-derived recombinant human LIN-28A
Met1-Asn209
Accession # Q9H9Z2
Specificity
Detects human LIN-28A in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIN28A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3757 in the following applications:

Publications
Read Publications using
AF3757 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIN-28A Antibody

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1)  is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-2379
Species: Hu, Mu, Rt, Bv, Pm
Applications: WB, Flow, ICC/IF, IHC
H00388112-B01P
Species: Hu
Applications: WB, ELISA
AF1997
Species: Hu
Applications: WB, ChIP, ICC
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
AF3158
Species: Mu
Applications: WB, ChIP, ICC
NBP2-32352
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-06520
Species: Hu, Mu
Applications: WB, IP, KO
NBP1-69368
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-42654
Species: Hu
Applications: WB, IP
NBP2-37357
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
NB600-235
Species: Hu, Mu, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-67121
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB110-40579
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, KD
NBP1-03349
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IP
H00057167-M03
Species: Hu, Mu
Applications: WB, ChIP, ELISA, ICC/IF, IHC, IHC-P
NBP2-43640
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-84863
Species: Hu
Applications: WB, IHC, IHC-P

Publications for LIN-28A Antibody (AF3757)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(4)
IHC
(1)
IHC-P
(1)
Western Blot
(4)
All Applications
Filter By Species
Human
(5)
Mouse
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF3757 Applications Species
RR Pandey, D Homolka, O Olotu, R Sachidanan, N Kotaja, RS Pillai Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12 Cell Rep, 2018;24(13):3423-3432.e4. 2018 [PMID: 30257204] (IHC, Mouse) IHC Mouse
R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata) Sci Rep, 2018;8(1):12187. 2018 [PMID: 30111816] (ICC, Primate - Macaca mulatta (Rhesus Macaque)) ICC Primate - Macaca mulatta (Rhesus Macaque)
AE Schaffer, MW Breuss, AO Caglayan, N Al-Sanaa, HY Al-Abdulwa, H Kaymakçala, C Y?lmaz, MS Zaki, RO Rosti, B Copeland, ST Baek, D Musaev, EC Scott, T Ben-Omran, A Karimineja, H Kayserili, F Mojahedi, M Kara, N Cai, JL Silhavy, S Elsharif, E Fenerciogl, BA Barshop, B Kara, R Wang, V Stanley, KN James, R Nachnani, A Kalur, H Megahed, F Incecik, S Danda, Y Alanay, E Faqeih, G Melikishvi, L Mansour, I Miller, B Sukhudyan, J Chelly, WB Dobyns, K Bilguvar, RA Jamra, M Gunel, JG Gleeson Biallelic loss of human CTNNA2, encoding ?N-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration Nat. Genet., 2018;0(0):. 2018 [PMID: 30013181] (ICC, Human) ICC Human
S Ramaswamy, N Tonnu, T Menon, BM Lewis, KT Green, D Wampler, PE Monahan, IM Verma Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX Cell Rep, 2018;23(5):1565-1580. 2018 [PMID: 29719266] (ICC, Human) ICC Human
SD Kasowitz, M Luo, J Ma, NA Leu, PJ Wang Embryonic lethality and defective male germ cell development in mice lacking UTF1 Sci Rep, 2017;7(1):17259. 2017 [PMID: 29222434] (Western Blot, Mouse) Western Blot Mouse
T Shibata, Y Fujita, H Ohno, Y Suzuki, K Hayashi, KR Komatsu, S Kawasaki, K Hidaka, S Yonehara, H Sugiyama, M Endo, H Saito Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate Nat Commun, 2017;8(1):540. 2017 [PMID: 28912471] (Western Blot, Human) Western Blot Human
AR Helsel, MJ Oatley, JM Oatley Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28392219] (IHC-P, Mouse) IHC-P Mouse
Makela J, Toppari J, Rivero-Muller A, Ventela S Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture. PLoS ONE, 2014;9(3):e90088. 2014 [PMID: 24619130] (ICC, Mouse) ICC Mouse
Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. PLoS ONE, 2010;5(12):e14397. 2010 [PMID: 21209933] (Western Blot, Human) Western Blot Human
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007;131(5):861-72. 2007 [PMID: 18035408] (Western Blot, Human) Western Blot Human

Review for LIN-28A Antibody (AF3757) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3757:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry LIN-28A AF3757
Enlarge
reviewed by:
Anonymous
ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotYFC011507B

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:200, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate
Secondary Manufacturer Cat#A-21432

Details

Detection NotesFluorescence Imaging, AF555 with mKo2 filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LIN-28A Antibody (AF3757) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF3757

Additional LIN-28A Products

Bioinformatics Tool for LIN-28A Antibody (AF3757)

Discover related pathways, diseases and genes to LIN-28A Antibody (AF3757). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for LIN-28A Antibody (AF3757)

Discover more about diseases related to LIN-28A Antibody (AF3757).
 

Pathways for LIN-28A Antibody (AF3757)

View related products by pathway.

PTMs for LIN-28A Antibody (AF3757)

Learn more about PTMs related to LIN-28A Antibody (AF3757).

Blogs on LIN-28A.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
08/04/2016
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol LIN28A
Uniprot
Novus 100% Guarantee