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LIN-28A Antibody

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Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN‑28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen ...read more
LIN‑28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. ...read more
LIN‑28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
Biotin
BAF3757
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Catalog# & Formulation Size Price
Carrier Free
AF3757-SP
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LIN-28A Antibody Summary

Immunogen
E. coli-derived recombinant human LIN-28A
Met1-Asn209
Accession # Q9H9Z2
Specificity
Detects human LIN-28A in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIN28A
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF3757 in the following applications:
Publications
Read Publications using
AF3757 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIN-28A Antibody

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1)  is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of BG01V human embryonic stem cells and Tera‑2 human embryonic lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Nanog Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1997) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Nanog at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA‑MD‑231 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HMGA2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HMGA2 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Western Blot: REXO1 Antibody [NBP1-84863] - analysis using Anti-REXO1 antibody NBP1-84863 (A) shows similar pattern to independent antibody NBP1-84862 (B).
Immunohistochemistry-Paraffin: REXO1 Antibody [NBP1-84863] - Staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.
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Publications for LIN-28A Antibody (AF3757)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: ICC, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(4)
IHC
(1)
IHC-P
(1)
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(4)
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Human
(5)
Mouse
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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Showing Publications 1 - 10 of 10.
Publications using AF3757 Applications Species
RR Pandey, D Homolka, O Olotu, R Sachidanan, N Kotaja, RS Pillai Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12 Cell Rep, 2018;24(13):3423-3432.e4. 2018 [PMID: 30257204] (IHC, Mouse) IHC Mouse
R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata) Sci Rep, 2018;8(1):12187. 2018 [PMID: 30111816] (ICC, Primate - Macaca mulatta (Rhesus Macaque)) ICC Primate - Macaca mulatta (Rhesus Macaque)
AE Schaffer, MW Breuss, AO Caglayan, N Al-Sanaa, HY Al-Abdulwa, H Kaymakçala, C Y?lmaz, MS Zaki, RO Rosti, B Copeland, ST Baek, D Musaev, EC Scott, T Ben-Omran, A Karimineja, H Kayserili, F Mojahedi, M Kara, N Cai, JL Silhavy, S Elsharif, E Fenerciogl, BA Barshop, B Kara, R Wang, V Stanley, KN James, R Nachnani, A Kalur, H Megahed, F Incecik, S Danda, Y Alanay, E Faqeih, G Melikishvi, L Mansour, I Miller, B Sukhudyan, J Chelly, WB Dobyns, K Bilguvar, RA Jamra, M Gunel, JG Gleeson Biallelic loss of human CTNNA2, encoding ?N-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration Nat. Genet., 2018;0(0):. 2018 [PMID: 30013181] (ICC, Human) ICC Human
S Ramaswamy, N Tonnu, T Menon, BM Lewis, KT Green, D Wampler, PE Monahan, IM Verma Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX Cell Rep, 2018;23(5):1565-1580. 2018 [PMID: 29719266] (ICC, Human) ICC Human
SD Kasowitz, M Luo, J Ma, NA Leu, PJ Wang Embryonic lethality and defective male germ cell development in mice lacking UTF1 Sci Rep, 2017;7(1):17259. 2017 [PMID: 29222434] (Western Blot, Mouse) Western Blot Mouse
T Shibata, Y Fujita, H Ohno, Y Suzuki, K Hayashi, KR Komatsu, S Kawasaki, K Hidaka, S Yonehara, H Sugiyama, M Endo, H Saito Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate Nat Commun, 2017;8(1):540. 2017 [PMID: 28912471] (Western Blot, Human) Western Blot Human
AR Helsel, MJ Oatley, JM Oatley Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28392219] (IHC-P, Mouse) IHC-P Mouse
Makela J, Toppari J, Rivero-Muller A, Ventela S Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture. PLoS ONE, 2014;9(3):e90088. 2014 [PMID: 24619130] (ICC, Mouse) ICC Mouse
Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. PLoS ONE, 2010;5(12):e14397. 2010 [PMID: 21209933] (Western Blot, Human) Western Blot Human
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007;131(5):861-72. 2007 [PMID: 18035408] (Western Blot, Human) Western Blot Human

Review for LIN-28A Antibody (AF3757) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF3757:
Filter by Applications
ICC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry LIN-28A AF3757
Enlarge 		4
reviewed by:
Anonymous
 ICC Mouse 08/04/2016
View

Summary

ApplicationImmunocytochemistry
Sample TestedR1 wild type Mouse embryonic Stem Cells
SpeciesMouse
LotYFC011507B

Blocking

Blocking DetailsBlocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

Dilution Ratio1:200, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Secondary Antibody

Secondary DescriptionDonkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate
Secondary Manufacturer Cat#A-21432

Details

Detection NotesFluorescence Imaging, AF555 with mKo2 filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
Fixation DetailsFixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
Wash DescriptionPBS,~1 min, 3 times

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FAQs for LIN-28A Antibody (AF3757) (0)

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Secondary Antibodies

 

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Biotin BAF3757
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Blogs on LIN-28A.

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Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.
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Immunocytochemistry LIN-28A AF3757
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 Anonymous
08/04/2016
Application: ICC
Species: Mouse
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Bioinformatics

Gene Symbol LIN28A
Uniprot