LIN-28A Antibody

Western blot shows lysates of JAR human choriocarcinoma cell line and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for LIN‑28A at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LIN‑28A was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LIN‑28A was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Human LIN‑28A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3757) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LIN-28A Antibody Summary

• Immunogen: E. coli-derived recombinant human LIN-28A
  Met1-Asn209
  Accession # Q9H9Z2
• Specificity: Detects human LIN-28A in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LIN28A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIN-28A Antibody

• CSDD1
• CSDD1lin-28A
• FLJ12457
• lin-28 homolog (C. elegans)
• lin-28 homolog A (C. elegans)
• LIN28
• LIN-28
• LIN28A
• LIN-28A
• LIN28RNA-binding protein LIN-28
• Tex17
• ZCCHC1
• ZCCHC1protein lin-28 homolog A
• Zinc finger CCHC domain-containing protein 1
• zinc finger, CCHC domain containing 1

Background

Human LIN-28 (Protein lin-28 homolog A; CSDD1, LIN28 and ZCCHC1)  is a 30 kDa (209 amino acids) cytoplasmic RNA-binding protein with an N-terminal cold shock domain and two C-terminal CCHC zinc finger domains. It is expressed by various undifferentiated embryonic cell types and is also present in adult cardiac and skeletal muscle. Expression of LIN-28 has been shown to be regulated by micro-RNA. Human LIN-28 shares 98% and 97% amino acid sequence homology with rat and mouse LIN-28, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIN-28A Antibody (AF3757)(10)

Publications using AF3757

• RR Pandey, D Homolka, O Olotu, R Sachidanan, N Kotaja, RS Pillai Exonuclease Domain-Containing 1 Enhances MIWI2 piRNA Biogenesis via Its Interaction with TDRD12 Cell Rep, 2018;24(13):3423-3432.e4. 2018 [PMID: 30257204] (IHC, Mouse)
• R Nakai, M Ohnuki, K Kuroki, H Ito, H Hirai, R Kitajima, T Fujimoto, M Nakagawa, W Enard, M Imamura Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata) Sci Rep, 2018;8(1):12187. 2018 [PMID: 30111816] (ICC, Primate - Macaca mulatta (Rhesus Macaque))
• AE Schaffer, MW Breuss, AO Caglayan, N Al-Sanaa, HY Al-Abdulwa, H Kaymakçala, C Y?lmaz, MS Zaki, RO Rosti, B Copeland, ST Baek, D Musaev, EC Scott, T Ben-Omran, A Karimineja, H Kayserili, F Mojahedi, M Kara, N Cai, JL Silhavy, S Elsharif, E Fenerciogl, BA Barshop, B Kara, R Wang, V Stanley, KN James, R Nachnani, A Kalur, H Megahed, F Incecik, S Danda, Y Alanay, E Faqeih, G Melikishvi, L Mansour, I Miller, B Sukhudyan, J Chelly, WB Dobyns, K Bilguvar, RA Jamra, M Gunel, JG Gleeson Biallelic loss of human CTNNA2, encoding ?N-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration Nat. Genet., 2018;0(0):. 2018 [PMID: 30013181] (ICC, Human)
• S Ramaswamy, N Tonnu, T Menon, BM Lewis, KT Green, D Wampler, PE Monahan, IM Verma Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX Cell Rep, 2018;23(5):1565-1580. 2018 [PMID: 29719266] (ICC, Human)
• SD Kasowitz, M Luo, J Ma, NA Leu, PJ Wang Embryonic lethality and defective male germ cell development in mice lacking UTF1 Sci Rep, 2017;7(1):17259. 2017 [PMID: 29222434] (Western Blot, Mouse)
• T Shibata, Y Fujita, H Ohno, Y Suzuki, K Hayashi, KR Komatsu, S Kawasaki, K Hidaka, S Yonehara, H Sugiyama, M Endo, H Saito Protein-driven RNA nanostructured devices that function in vitro and control mammalian cell fate Nat Commun, 2017;8(1):540. 2017 [PMID: 28912471] (Western Blot, Human)
• AR Helsel, MJ Oatley, JM Oatley Glycolysis-Optimized Conditions Enhance Maintenance of Regenerative Integrity in Mouse Spermatogonial Stem Cells during Long-Term Culture Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28392219] (IHC-P, Mouse)
• Makela J, Toppari J, Rivero-Muller A, Ventela S Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture. PLoS ONE, 2014;9(3):e90088. 2014 [PMID: 24619130] (ICC, Mouse)
• Plews JR, Li J, Jones M, Moore HD, Mason C, Andrews PW, Na J Activation of pluripotency genes in human fibroblast cells by a novel mRNA based approach. PLoS ONE, 2010;5(12):e14397. 2010 [PMID: 21209933] (Western Blot, Human)
• Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007;131(5):861-72. 2007 [PMID: 18035408] (Western Blot, Human)

Review for LIN-28A Antibody (AF3757) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous ICC Mouse 08/04/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: R1 wild type Mouse embryonic Stem Cells
• Species: Mouse
• Lot: YFC011507B

Blocking

• Blocking Details: Blocking: 0.1% Triton X in PBS with 5% donkey serum, 1 hr at RT

Primary Anitbody

• Dilution Ratio: 1:200, 14 hrs, 4 C, Buffer: 0.1% Triton X + 5% donkey serum in PBS

Secondary Antibody

• Secondary Description: Donkey anti-Goat IgG (H+L) Secondary Antibody, Alexa Fluor® 555 conjugate
• Secondary Manufacturer Cat#: A-21432

Details

• Detection Notes: Fluorescence Imaging, AF555 with mKo2 filter cube, Nikon Eclipse Ti microscope, Exposure time: 1 sec
• Fixation Details: Fixed for 10 mins with 4% formaldehyde at room temperature. Permeablization with 0.2% TritonX in PBS for 15 mins at RT
• Wash Description: PBS,~1 min, 3 times

Bioinformatics

• Gene Symbol: LIN28A
• Uniprot:
  • Q9H9Z2()