c-Myc Antibody (9E10)

Images

 
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained ...read more
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Analysis of Alexa Fluor (R) 647 conjugate of NB600-302. An intracellular stain was performed on HeLa cells with c-MYC antibody (9E10) NB600-302AF647 (blue) and a ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - Analysis of transgenic Eu-Myc+ mouse splenocytes using c-Myc PE conjugated antibody. Image from verified customer review.
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
9E10
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

c-Myc Antibody (9E10) Summary

Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 1:200
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Jurkat Lysate (NB800-PC2)
c-Myc Lysate (NBL1-13414)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB600-302 in the following applications:

Publications
Read Publications using
NB600-302 in the following applications:

  • 1 publication
  • 1 publication
  • 4 publications
  • IP
    6 publications
  • WB
    9 publications

Reactivity Notes

Human, mouse and Drosophila.

Alternate Names for c-Myc Antibody (9E10)

  • 9E10 anti-Myc
  • 9E10 Myc
  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for c-Myc Antibody (NB600-302)(22)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ELISA, FLOW, ICC/IF, IP, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
ICC/IF
(4)
IP
(6)
WB
(9)
All Applications
Filter By Species
Human
(7)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using NB600-302 Applications Species
da Costa JP, Carvalhais V, Amado F et al. Anti-tumoral activity of human salivary peptides

Details:
c-Myc antibody used for Western blot on human cell lines from MG63, HT29, T24 and T-47 cells that were treated with different concentrations of GG peptide (Fig 4). Peptides. 2015 Jul 23 [PMID:26211891] (WB, Human)
WB Human
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun.

Details:
Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C. Nat Commun 2015 Apr 10 [PMID:25857523] (WB, Mouse)
WB Mouse
Iampietro M, Morissette G, Gravel A, Flamand L. Inhibition of Interleukin-2 Gene Expression by Human Herpesvirus 6B U54 Tegument Protein. J Virol. 2014 Aug 13 [PMID:25122797] (WB, Human) WB Human
Pekkurnaz G, Trinidad JC, Wang X et al. Glucose Regulates Mitochondrial Motility via Milton Modification by O-GlcNAc Transferase.

Details:
COS7 cells transfected with empty vector or myc-hMilton1 (Figure S3). Cell 2014 Jul 03 [PMID:24995978] (ICC/IF)
ICC/IF
Barnett TC, Liebl D, Seymour LM et al. The Globally Disseminated M1T1 Clone of Group A Streptococcus Evades Autophagy for Intracellular Replication. Cell Host Microbe 2013 Dec 11 [PMID:24331465] (ICC/IF) ICC/IF
Hsieh YT, Chou MM, Chen HC, Tseng JJ. IMP1 promotes choriocarcinoma cell migration and invasion through the novel effectors RSK2 and PPME1. Gynecol Oncol. 2013 Oct [PMID:23911878]
Hubbi ME, Hu H, Kshitiz NF et al. Sirtuin-7 Inhibits the Activity of Hypoxia-Inducible Factors J Biol Chem 2013 June 9 [PMID:23750001]
Megill A, Lee T, Dibattista AM et al. A Tetra(Ethylene Glycol) Derivative of Benzothiazole Aniline Enhances Ras-Mediated Spinogenesis. J Neurosci 2013 May 29 [PMID:23719799] (ICC/IF) ICC/IF
Wang H, Shi LZ, Wong CC et al. The Interaction of CtIP and Nbs1 Connects CDK and ATM to Regulate HR-Mediated Double-Strand Break Repair. PLoS Genet 2013 Feb [PMID:23468639] (WB) WB
Lu CS, Truong LN, Aslanian A et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012 Oct 31 [PMID:23115235] (IP) IP
Show All 22 Publications.

Reviews for c-Myc Antibody (NB600-302) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.
We have 3 review tested in 1 application: WB.

Reviews using NB600-302:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedJurkat T-Cells
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: sigma anti-mouse

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood!
 
reviewed by:
Astrid Spannhoff
WB Human 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 10 ul
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9320

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 1 minute, Specific Bands: 53 kDa
 
reviewed by:
Anonymous
WB Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-302) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB600-302AF405
Alexa Fluor 488 Labeled NB600-302AF488
Alexa Fluor 647 Labeled NB600-302AF647
Alexa Fluor 700 Labeled NB600-302AF700
Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 650 Labeled NB600-302C
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
HRP Labeled NB600-302H
PE Labeled NB600-302PE
PerCP Labeled NB600-302PCP

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Blogs on c-Myc. Showing 1-10 of 13 blog posts - Show all blog posts.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.

Contact Information

Product PDFs

Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
11/30/2011
Application: WB
Species: Human

 
Astrid Spannhoff
11/23/2010
Application: WB
Species: Human

 
Anonymous
08/10/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

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