c-Myc Antibody (9E10)

Images

 
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. ...read more
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, ...read more
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, DrSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
9E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

c-Myc Antibody (9E10) Summary

Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 1:200
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Flow (Intracellular)
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation. Use in immunoblotting reported in scientific literature (PMID: 23750001).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Jurkat Whole Cell Lysate
c-Myc Overexpression Lysate
c-Myc Knockout 293T Cell Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB600-302 in the following applications:

Publications
Read Publications using
NB600-302 in the following applications:

  • 1 publication
  • 1 publication
  • 6 publications
  • IHC
    1 publication
  • IP
    7 publications
  • WB
    12 publications

Reactivity Notes

Human, mouse and Drosophila.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for c-Myc Antibody (9E10)

  • 9E10 anti-Myc
  • 9E10 Myc
  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N.,... Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ChIP, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for c-Myc Antibody (NB600-302)(30)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA, FLOW, ICC/IF, IHC, IP, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
ICC/IF
(6)
IHC
(1)
IP
(7)
WB
(12)
All Applications
Filter By Species
Human
(9)
Mouse
(7)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using NB600-302 Applications Species
Schuchner S, Behm C, Mudrak I, Ogris E The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context Sci Signal Jan 28 2020 [PMID: 31992583] (WB) WB
Pedrosa AR, Bodrug N, Gomez-Escudero J et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res. 2019 Jun 12 [PMID: 31189647] (IP, WB, Mouse) IP, WB Mouse
Ida K, Miyamoto T, Higuchi S et al. Effectiveness of a genetic test panel designed for gynecological cancer: an exploratory study Med. Oncol. May 29 2019 [PMID: 31139954] (IHC, Human) IHC Human
Morioka S, Perry JSA, Raymond MH et al. Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. Nov 1 2018 [PMID: 30464343] (ICC/IF, Mouse) ICC/IF Mouse
Wu J, Fan W, Ma L, Geng X. miR-708-5p promotes fibroblast-like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by the inhibition of Wnt3a/B-catenin pathway Neuron Oct 10 2018 [PMID: 30349197] (WB) WB
Rodriguez-Muela N, Parkhitko A, Grass T et al. Blocking p62/SQSTM1-dependent SMN degradation ameliorates Spinal Muscular Atrophy disease phenotypes. J. Clin. Invest. Apr 19 2018 [PMID: 29672276] (Mouse) Mouse
Mahadevan S, Sathappan V, Utama B et al. Maternally expressed NLRP2 links the subcortical maternal complex (SCMC) to fertility, embryogenesis and epigenetic reprogramming. Sci Rep. Mar 20 2017 [PMID: 28317850] (ICC/IF, Mouse) ICC/IF Mouse
van der Kant R, Jonker CT, Wijdeven RH et al. Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity J Biol Chem 2015 Dec 18 [PMID: 26463206] (Human)

Details:
This reference used the HRP version of NB600-302.
Human
da Costa JP, Carvalhais V, Amado F et al. Anti-tumoral activity of human salivary peptides Peptides. 2015 Jul 23 [PMID: 26211891] (WB, Human)

Details:
c-Myc antibody used for Western blot on human cell lines from MG63, HT29, T24 and T-47 cells that were treated with different concentrations of GG peptide (Fig 4).
WB Human
Jang KJ, Mano H, Aoki K et al. Mitochondrial function provides instructive signals for activation-induced B-cell fates Nat Commun. Nat Commun 2015 Apr 10 [PMID: 25857523] (WB, Mouse)

Details:
Using the DyLight 650 conjugated version of NB600-302, catalog number NB600-302C.
WB Mouse
Show All 30 Publications.

Reviews for c-Myc Antibody (NB600-302) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB600-302:
Filter by Applications
ELISA
(1)
WB
(3)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Phil Hill
ELISA Human 05/31/2019
View

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman
LotMAB8594
 
reviewed by:
Anonymous
WB Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedJurkat T-Cells
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Secondary Antibody

Secondary DescriptionSecondary Ab: sigma anti-mouse

Details

Detection NotesDetection Method: ECL

Comments

CommentsGood!
 
reviewed by:
Astrid Spannhoff
WB Human 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 10 ul
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-mouse IgG, Secondary Ab Dilution Ratio: 1:5000
Secondary Manufacturer Cat#NA9320

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 1 minute, Specific Bands: 53 kDa
 
reviewed by:
Anonymous
WB Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-302) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-302AF350
Alexa Fluor 405 NB600-302AF405
Alexa Fluor 488 NB600-302AF488
Alexa Fluor 532 NB600-302AF532
Alexa Fluor 594 NB600-302AF594
Alexa Fluor 647 NB600-302AF647
Alexa Fluor 700 NB600-302AF700
Alexa Fluor 750 NB600-302AF750
Allophycocyanin NB600-302APC
Biotin NB600-302B
DyLight 350 NB600-302UV
DyLight 405 NB600-302V
DyLight 488 NB600-302G
DyLight 550 NB600-302R
DyLight 594 NB600-302DL594
DyLight 650 NB600-302C
DyLight 680 NB600-302FR
DyLight 755 NB600-302IR
FITC NB600-302F
HRP NB600-302H
Janelia Fluor 549 NB600-302JF549
Janelia Fluor 646 NB600-302JF646
PE NB600-302PE
PerCP NB600-302PCP

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Blogs on c-Myc. Showing 1-10 of 15 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
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Recent Reviews

4.3
5
2
4
1
3
1
2
0
1
0

 
Phil Hill
05/31/2019
Application: ELISA
Species: Human

 
Anonymous
11/30/2011
Application: WB
Species: Human

 
Astrid Spannhoff
11/23/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot
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