c-Myc Antibody (9E10) - BSA Free

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Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. ...read more
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, ...read more
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Flow cytometric analysis of ROS and the mitochondrial status. Western blotting analysis of unsorted (W) and sorted P1 and P2 cells. Data shown are representative of at ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, DrSpecies Glossary
Applications WB, Simple Western, ChIP, ELISA, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA
Clone
9E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

c-Myc Antibody (9E10) - BSA Free Summary

Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1:50-1:200
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Simple Western 1:200
  • Western Blot 0.5-2.0 ug/ml
Application Notes
Use in PLA reported in scientific literature (PMID:33298911). This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, ICC/IF, IHC, IHC-Frozen, IHC-Paraffin and Immunoprecipitation. Use in immunoblotting reported in scientific literature (PMID: 23750001).
Use in Sandwich ELISA reported in scientific literature (PMID:33122198).
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:100; matrix was 12-230 kDa.
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
c-Myc Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB600-302 in the following applications:

Publications
Read Publications using
NB600-302 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for c-Myc Antibody (9E10) - BSA Free

  • 9E10 anti-Myc
  • 9E10 Myc
  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Myc Antibody (NB600-302)(38)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 9 applications: ELISA, FLOW, ICC/IF, IHC, IHC-P, IP, PLA, Sandwich ELISA, WB.


Filter By Application
ELISA
(1)
FLOW
(1)
ICC/IF
(6)
IHC
(1)
IHC-P
(1)
IP
(8)
PLA
(1)
Sandwich ELISA
(1)
WB
(16)
All Applications
Filter By Species
Human
(10)
Mouse
(9)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NB600-302 Applications Species
Harbauer AB, Hees JT, Wanderoy S et al. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy Neuron Feb 19 2022 [PMID: 35216662] (IP, Human) IP Human
Bewley MC, Gautam L, Gowda DC, Flanagan JM Molecular architecture and domain arrangement of the placental malaria protein VAR2CSA suggests a model for receptor binding bioRxiv Jan 1 2020
Li K, Wang F, Yang ZN et al. TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation Nature communications Dec 9 2020 [PMID: 33298911] (PLA, WB, Mouse) PLA, WB Mouse
Bewley MC, Gautam L, Jagadeeshaprasad MG et al. Molecular architecture and domain arrangement of the placental malaria protein VAR2CSA suggests a model for carbohydrate binding J Biol Chem Oct 29 2020 [PMID: 33122198] (Sandwich ELISA, Bovine) Sandwich ELISA Bovine
Shen M, Wei Y, Kim H Et al. Small-molecule inhibitors that disrupt the MTDH-SND1 complex suppress breast cancer progression and metastasis Nature Cancer Nov 29 2021 (IHC-P, WB, Mouse) IHC-P, WB Mouse
Kumagai S, Koyama S, Itahashi K et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer cell Jan 25 2022 [PMID: 35090594]
Zhao L, Han X, Lu J et al. A highly potent PROTAC androgen receptor (AR) degrader ARD-61 effectively inhibits AR-positive breast cancer cell growth in vitro and tumor growth in vivo Neoplasia (New York, N.Y.) Oct 1 2020 [PMID: 32928363] (WB) WB
Schuchner S, Behm C, Mudrak I, Ogris E The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context Sci Signal Jan 28 2020 [PMID: 31992583] (WB) WB
Pedrosa AR, Bodrug N, Gomez-Escudero J et al. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861 Cancer Res. 2019 Jun 12 [PMID: 31189647] (IP, WB, Mouse) IP, WB Mouse
Ida K, Miyamoto T, Higuchi S et al. Effectiveness of a genetic test panel designed for gynecological cancer: an exploratory study Med. Oncol. May 29 2019 [PMID: 31139954] (IHC, Human) IHC Human
Show All 38 Publications.

Reviews for c-Myc Antibody (NB600-302) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB600-302:
Filter by Applications
ELISA
(1)
Western Blot
(3)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
ELISA Human 05/31/2019
View

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman
LotMAB8594
  5
reviewed by:
Ju-Pi Li
Western Blot Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedJurkat T-Cells
SpeciesHuman
  4
reviewed by:
Verified Customer
Western Blot Human 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 10 ul
SpeciesHuman
  5
reviewed by:
Maimon Hubbi
Western Blot Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-302). (Showing 1 - 2 of 2 FAQs).

  1. This antibody NB600-302 is raised in Mouse. It is suggesting that it will work in Mouse system. Please explain, since I am trying to implement this antibody in the mouse system.
    • NB600-302 is a Mouse Monoclonal antibody, and it is fine for use on Mouse samples. Generally, there are no issues with mouse on mouse antibody if there are adequate blocking and washing steps.
  2. I am interested in using clone 9E10 and would like to confirm if it recognizes p64 version of the protein as well as p67 version of the protein
    • Yes, it recognizes both form of c-Myc protein (p64 and p67), based on literature and other scientific material.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for c-Myc Antibody (NB600-302)

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Blogs on c-Myc. Showing 1-10 of 15 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription ...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on ...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
Omicron Variant Antibodies

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Recent Reviews

4.3
5
2
4
1
3
1
2
0
1
0

 
Verified Customer
05/31/2019
Application: ELISA
Species: Human

 
Ju-Pi Li
11/30/2011
Application: Western Blot
Species: Human

 
Verified Customer
11/23/2010
Application: Western Blot
Species: Human

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot