c-Myc Antibody (9E10) - BSA Free

Images

 
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. ...read more
Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, ...read more
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa ...read more
Western Blot: c-Myc Antibody (9E10) - BSA Free [NB600-302] - Western blotting analysis of unsorted (W) and sorted ROSl degrees w(L) and ROShigh(H) cells. Data shown are representative of at least two independent ...read more
Immunocytochemistry/ Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302PE (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Flow cytometric analysis of ROS and the mitochondrial status. Western blotting analysis of unsorted (W) and sorted P1 and P2 cells. Data shown are representative of at ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow (Intracellular): c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302F (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, DrSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, Func, ICC/IF, IHC, IP, WB, ChIP
Clone
9E10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

c-Myc Antibody (9E10) - BSA Free Summary

Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc Antibody (9E10). [UniProt# P01106]
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • ELISA 1:100-1:2000
  • Flow (Intracellular)
  • Flow Cytometry 1:50-1:200. Use reported in scientific literature (PMID 21315712)
  • Immunoblotting reported in scientific literature (PMID 23750001)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay reported in scientific literature (PMID 33298911)
  • Sandwich ELISA reported in scientific literature (PMID 33122198)
  • Simple Western 1:200
  • Western Blot 0.5-2.0 ug/ml
Application Notes
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:100; matrix was 12-230 kDa.
Theoretical MW
48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Jurkat Whole Cell Lysate
c-Myc Overexpression Lysate
Reviewed Applications
Read 4 Reviews rated 4.3
using
NB600-302 in the following applications:

Publications
Read Publications using
NB600-302 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for c-Myc Antibody (9E10) - BSA Free

  • 9E10 anti-Myc
  • 9E10 Myc
  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • cMyc
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is a multifunctional, nuclear phosphoprotein that functions as a transcription factor with a theoretical molecular weight of 62kDa. However, c-Myc is extremely labile and is degraded very quickly even in extracts prepared with boiling SDS sample buffer, such that a molecular weight of ~40 kDa has been observed. c-Myc is part of a heterodimeric complex with Max that acts as a potent transcriptional activator. c-Myc is modified by glycosylation and phosphorylation and has been shown to interact with numerous proteins including SMAD2, SMAD3, LSD1/KDM1A, MAD, and Sp1 (1).

A basic Helix-Loop-Helix, Leucine Zipper domain (bHLH/LZ), designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with other bHLH-Zip proteins, Mad and Mxi1. c-Myc plays a role in cell cycle progression, apoptosis, cellular transformation and angiogenesis (2). Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of cancers including B-cell Lymphomas, acute myeloid leukemia, glioblastoma, stomach adenocarcinoma, and prostate adenocarcinoma (3).

References

1. Wilkinson, D. S., Tsai, W. W., Schumacher, M. A., & Barton, M. C. (2008). Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming-growth-factor-beta-mediated transcription repression. Mol Cell Biol, 28(6), 1988-1998. doi:10.1128/mcb.01442-07

2. Pedrosa, A. R., Bodrug, N., Gomez-Escudero, J., Carter, E. P., Reynolds, L. E., Georgiou, P. N., . . . Hodivala-Dilke, K. M. (2019). Tumor Angiogenesis Is Differentially Regulated by Phosphorylation of Endothelial Cell Focal Adhesion Kinase Tyrosines-397 and -861. Cancer Res, 79(17), 4371-4386. doi:10.1158/0008-5472.Can-18-3934

3. Nagasaka, M., Tsuzuki, K., Ozeki, Y., Tokugawa, M., Ohoka, N., Inoue, Y., & Hayashi, H. (2019). Lysine-Specific Demethylase 1 (LSD1/KDM1A) Is a Novel Target Gene of c-Myc. Biol Pharm Bull, 42(3), 481-488. doi:10.1248/bpb.b18-00892

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for c-Myc Antibody (NB600-302)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Drosophila.

We have publications tested in 9 applications: B/N, ELISA, FLOW, ICC/IF, IF/IHC, IP, PLA, Sandwich ELISA, WB.


Filter By Application
B/N
(2)
ELISA
(2)
FLOW
(1)
ICC/IF
(6)
IF/IHC
(1)
IP
(8)
PLA
(1)
Sandwich ELISA
(1)
WB
(19)
All Applications
Filter By Species
Human
(11)
Mouse
(8)
Bovine
(1)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB600-302 Applications Species
Cheramangalam RN, Anand T, Pandey P et al. Bendless is essential for PINK1-Park mediated Mitofusin degradation under mitochondrial stress caused by loss of LRPPRC PLoS genetics 2023-04-01 [PMID: 37098042] (WB, Drosophila) WB Drosophila
Graziani V, Garcia AR, Alcolado LS et al. Metabolic rewiring in MYC-driven medulloblastoma by BET-bromodomain inhibition Scientific Reports 2023-01-23 [PMID: 36690651] (WB, B/N) WB, B/N
Jagadeeshaprasad MG, Gautam L, Bewley MC et al. Disulfide bond and crosslinking analyses reveal inter-domain interactions that contribute to the rigidity of placental malaria VAR2CSA structure and formation of CSA binding channel International journal of biological macromolecules 2022-12-05 [PMID: 36470436] (ELISA) ELISA
Jezek M, Sun W, Negesse MY et al. Set1 regulates telomere function via H3K4 methylation-dependent and -independent pathways and calibrates the abundance of telomere maintenance factors Molecular Biology of the Cell 2023-01-01 [PMID: 36416860] (WB) WB
Edwards-Hicks J, Su H, Mangolini M et al. MYC sensitises cells to apoptosis by driving energetic demand Nature communications 2022-08-09 [PMID: 35945217] (WB, Human) WB Human
Harbauer AB, Hees JT, Wanderoy S et al. Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy Neuron 2022-02-19 [PMID: 35216662] (IP, Human) IP Human
Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594]

Details:
Citation using the PE version of this antibody.
Li K, Wang F, Yang ZN et al. TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation Nature communications 2020-12-09 [PMID: 33298911] (WB, PLA, Mouse) WB, PLA Mouse
Wang F, Gao Y, Xue S et al. SCARB2 Drives Hepatic Carcinoma Initiation by Supporting Cancer Stem Cell Traits and Enhancing MYC Transcriptional Activity Research Square 2022-02-17 (WB) WB
Wang F, Gao Y, Xue S et al. SCARB2 drives hepatocellular carcinoma tumor initiating cells via enhanced MYC transcriptional activity Nat Commun 2023-09-22 [PMID: 37739936]
Show All 44 Publications.

Reviews for c-Myc Antibody (NB600-302) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Human.

Reviews using NB600-302:
Filter by Applications
ELISA
(1)
WB
(3)
All Applications
Filter by Species
Human
(4)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Phil Hill
ELISA Human 05/31/2019
View

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman
LotMAB8594
  5
reviewed by:
Verified Customer
WB Human 11/30/2011
View

Summary

ApplicationWestern Blot
Sample TestedJurkat T-Cells
SpeciesHuman
  4
reviewed by:
Astrid Spannhoff
WB Human 11/23/2010
View

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 10 ul
SpeciesHuman
  5
reviewed by:
Verified Customer
WB Human 08/10/2010
View

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
SpeciesHuman

Product General Protocols

View specific protocols for c-Myc Antibody (NB600-302): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for c-Myc Antibody (NB600-302). (Showing 1 - 2 of 2 FAQs).

  1. This antibody NB600-302 is raised in Mouse. It is suggesting that it will work in Mouse system. Please explain, since I am trying to implement this antibody in the mouse system.
    • NB600-302 is a Mouse Monoclonal antibody, and it is fine for use on Mouse samples. Generally, there are no issues with mouse on mouse antibody if there are adequate blocking and washing steps.
  2. I am interested in using clone 9E10 and would like to confirm if it recognizes p64 version of the protein as well as p67 version of the protein
    • Yes, it recognizes both form of c-Myc protein (p64 and p67), based on literature and other scientific material.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional c-Myc Products

Research Areas for c-Myc Antibody (NB600-302)

Find related products by research area.

Blogs on c-Myc. Showing 1-10 of 15 blog posts - Show all blog posts.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

MYC - A human oncogene with valuable laboratory applications
Myc is a basic helix-loop-helix zipper transcription factor that regulates a network of many hundreds of genes. Myc up-regulates the expression of many genes involved in cell growth and proliferation such as ribosome biogenesis and protein synthesi...  Read full blog post.

c-Myc - transcription factor and oncogene
c-Myc is a protein of the Myc family of transcription factors (c-Myc, B-Myc, L-Myc, N-Myc, and s-Myc) encoded by the MYC proto-oncogene. c-Myc was first discovered as the cellular homolog of the retroviral v-Myc oncogene. c-Myc is a transcription ...  Read full blog post.

c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample on ...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2A....  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.
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Recent Reviews

4.3
5
2
4
1
3
1
2
0
1
0

 
Phil Hill
05/31/2019
Application: ELISA
Species: Human

 
Verified Customer
11/30/2011
Application: WB
Species: Human

 
Astrid Spannhoff
11/23/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot