Recombinant Human BMP-4 Protein

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Recombinant Human BMP‑4 (Catalog # 314-BP) induces BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL.
1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-4 Protein Summary

Details of Functionality
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293-Arg408
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions
Publications
Read Publications using
314-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-4 Protein

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

 

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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314-BP
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Applications: Bioactivity

Publications for BMP-4 (314-BP)(496)

We have publications tested in 18 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Equine, Northern White Rhinoceros, Ovine, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Rhinoceros, Transgenic Mouse, Xenopus, Zebrafish.

We have publications tested in 8 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Vivo, Surface Plasmon Resonance, Tissue Culture, Western Blot.


Filter By Application
Binding Assay
(3)
Bioassay
(443)
Cell Culture
(33)
Differentiation
(4)
In Vivo
(13)
Surface Plasmon Resonance
(4)
Tissue Culture
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(394)
Mouse
(66)
Rat
(6)
Bovine
(5)
Chicken
(15)
Equine
(1)
Northern White Rhinoceros
(1)
Ovine
(1)
Porcine
(2)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Rabbit
(2)
Rhinoceros
(1)
Transgenic Mouse
(1)
Xenopus
(5)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 496. Show All 496 Publications.
Publications using 314-BP Applications Species
Zhang, H;Kafeiti, N;Lee, S;Masarik, K;Zheng, H;Zhan, H; Unlocking the Role of Endothelial MPL Receptor and JAK2V617F Mutation: Insights into Cardiovascular Dysfunction in MPNs and CHIP bioRxiv : the preprint server for biology 2023-07-13 [PMID: 37503259] (Bioassay, Human) Bioassay Human
Ai, Z;Niu, B;Yin, Y;Xiang, L;Shi, G;Duan, K;Wang, S;Hu, Y;Zhang, C;Zhang, C;Rong, L;Kong, R;Chen, T;Guo, Y;Liu, W;Li, N;Zhao, S;Zhu, X;Mai, X;Li, Y;Wu, Z;Zheng, Y;Fu, J;Ji, W;Li, T; Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids Cell research 2023-07-17 [PMID: 37460804] (Bioassay, Human) Bioassay Human
Tresenrider, A;Sridhar, A;Eldred, KC;Cuschieri, S;Hoffer, D;Trapnell, C;Reh, TA; Single-cell sequencing of individual retinal organoids reveals determinants of cell fate heterogeneity bioRxiv : the preprint server for biology 2023-06-03 [PMID: 37398481] (Bioassay, Human) Bioassay Human
Alber, AB;Marquez, HA;Ma, L;Kwong, G;Thapa, BR;Villacorta-Martin, C;Lindstrom-Vautrin, J;Bawa, P;Wang, F;Luo, Y;Ikonomou, L;Shi, W;Kotton, DN; Directed differentiation of mouse pluripotent stem cells into functional lung-specific mesenchyme Nature communications 2023-06-13 [PMID: 37311756] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Frum, T;Hsu, PP;Hein, RFC;Conchola, AS;Zhang, CJ;Utter, OR;Anand, A;Zhang, Y;Clark, SG;Glass, I;Sexton, JZ;Spence, JR; Opposing roles for TGF?- and BMP-signaling during nascent alveolar differentiation in the developing human lung bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205521] (Bioassay, Human) Bioassay Human
Maeda, Y;Isomura, A;Masaki, T;Kageyama, R; Differential cell-cycle control by oscillatory versus sustained Hes1 expression via p21 Cell reports 2023-05-17 [PMID: 37200191] (Bioassay, Human) Bioassay Human
Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW; A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Nature protocols 2023-05-10 [PMID: 37165073] (Bioassay, Human) Bioassay Human
Majd, H;Amin, S;Ghazizadeh, Z;Cesiulis, A;Arroyo, E;Lankford, K;Majd, A;Farahvashi, S;Chemel, AK;Okoye, M;Scantlen, MD;Tchieu, J;Calder, EL;Le Rouzic, V;Shibata, B;Arab, A;Goodarzi, H;Pasternak, G;Kocsis, JD;Chen, S;Studer, L;Fattahi, F; Deriving Schwann cells from hPSCs enables disease modeling and drug discovery for diabetic peripheral neuropathy Cell stem cell 2023-05-04 [PMID: 37146583] (Bioassay, Human) Bioassay Human
S Teague, G Primavera, B Chen, E Freeburne, H Khan, K Jo, C Johnson, I Heemskerk The time integral of BMP signaling determines fate in a stem cell model for early human development bioRxiv : the preprint server for biology, 2023-04-10;0(0):. 2023-04-10 [PMID: 37090515] (Bioassay, Human) Bioassay Human
A Gehrlein, V Udayar, N Anastasi, ML Morella, I Ruf, D Brugger, S von der Ma, R Thoma, A Rufer, D Heer, N Pfahler, A Jochner, J Niewoehner, L Wolf, M Fueth, M Ebeling, R Villaseñor, Y Zhu, MC Deen, X Shan, Z Ehsaei, V Taylor, E Sidransky, DJ Vocadlo, PO Freskgård, R Jagasia Targeting neuronal lysosomal dysfunction caused by beta-glucocerebrosidase deficiency with an enzyme-based brain shuttle construct Nature Communications, 2023-04-12;14(1):2057. 2023-04-12 [PMID: 37045813] (Bioassay, Human) Bioassay Human
Show All 496 Publications.

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Bioinformatics

Gene Symbol BMP4
Uniprot