Recombinant Human BMP-4 Protein

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1 ug/lane of Recombinant Human BMP-4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 22-25 kDa and 37-41 kDa, respectively. ...read more
Recombinant Human BMP-4 (Catalog #314-BP/CF) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The ED50 for this effect is 2.5-15 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

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Recombinant Human BMP-4 Protein Summary

Additional Information
A New Animal Free BMP-4 is Available!
Details of Functionality
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.70-7.00 ng/mL. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is 3.00-30.0 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human BMP-4 protein
Ser293-Arg408
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP4
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
19-24 kDa, reducing conditions
35-41 kDa, non-reducing conditions
Publications
Read Publications using
314-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 50-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-4 Protein

  • BMP2B
  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • MCOPS6
  • OFC11
  • ZYME

Background

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7
disulfide-linked heterodimer (7-9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

 

  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Wozney, J. et al. (1988) Science 242:1528.
  7. Cui, Y. et al. (1998) EMBO J. 17:4735.
  8. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  9. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  10. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  11. Chen, D. et al. (2004) Growth Factors 22:233.
  12. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  13. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  14. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  15. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.

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314-BP
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Applications: Bioactivity

Publications for BMP-4 (314-BP)(400)

We have publications tested in 14 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Equine, Ovine, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque), Rabbit, Xenopus, Zebrafish.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Ovo, In Vivo, Surface Plasmon Resonance, Tissue Culture, Western Blot.


Filter By Application
Binding Assay
(3)
Bioassay
(352)
Cell Culture
(23)
Differentiation
(2)
In Ovo
(1)
In Vivo
(8)
Surface Plasmon Resonance
(3)
Tissue Culture
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(309)
Mouse
(42)
Rat
(5)
Bovine
(5)
Chicken
(15)
Equine
(1)
Ovine
(1)
Porcine
(2)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(2)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
(1)
Rabbit
(2)
Xenopus
(5)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 400. Show All 400 Publications.
Publications using 314-BP Applications Species
A Mithal, AJ Hume, J Lindstrom-, C Villacorta, J Olejnik, E Bullitt, A Hinds, E Mühlberger, G Mostoslavs Human Pluripotent Stem Cell-Derived Intestinal Organoids Model SARS-CoV-2 Infection Revealing a Common Epithelial Inflammatory Response Stem Cell Reports, 2021;16(4):940-953. 2021 [PMID: 33852884] (Bioassay, Human) Bioassay Human
S Io, M Kabata, Y Iemura, K Semi, N Morone, A Minagawa, B Wang, I Okamoto, T Nakamura, Y Kojima, C Iwatani, H Tsuchiya, B Kaswandy, E Kondoh, S Kaneko, K Woltjen, M Saitou, T Yamamoto, M Mandai, Y Takashima Capturing human trophoblast development with naive pluripotent stem cells in�vitro Cell Stem Cell, 2021;28(6):1023-1039.e13. 2021 [PMID: 33831365] (Bioassay, Human) Bioassay Human
G Campostrin, V Meraviglia, E Giacomelli, RWJ van Helden, L Yiangou, RP Davis, M Bellin, VV Orlova, CL Mummery Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells Nature Protocols, 2021;0(0):. 2021 [PMID: 33772245] (Bioassay, Human) Bioassay Human
AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D Nature Protocols, 2021;0(0):. 2021 [PMID: 33649566] (Bioassay, Human) Bioassay Human
W Yuan, Z Yao, V Veerapandi, X Yang, X Wang, D Chen, L Ma, C Li, Y Zheng, F Luo, XY Zhao The histone demethylase KDM2B regulates human primordial germ cell-like cells specification International journal of biological sciences, 2021;17(2):527-538. 2021 [PMID: 33613110] (Bioassay, Human) Bioassay Human
S Monkley, JK Krishnaswa, M Göransson, M Clausen, J Meuller, K Thörn, R Hicks, S Delaney, L Stjernborg Optimised generation of iPSC-derived macrophages and dendritic cells that are functionally and transcriptionally similar to their primary counterparts PLoS ONE, 2020;15(12):e0243807. 2020 [PMID: 33332401] (Cell Culture, Human) Cell Culture Human
J Qiu, S Nordling, HH Vasavada, EC Butcher, KK Hirschi Retinoic Acid Promotes Endothelial Cell Cycle Early G1 State to Enable Human Hemogenic Endothelial Cell Specification Cell Rep, 2020;33(9):108465. 2020 [PMID: 33264627] (Cell Culture, Human) Cell Culture Human
T Chitiashvi, I Dror, R Kim, FM Hsu, R Chaudhari, E Pandolfi, D Chen, S Liebscher, K Schenke-La, K Plath, A Clark Female human primordial germ cells display X-chromosome dosage compensation despite the absence of X-inactivation Nature Cell Biology, 2020;22(12):1436-1446. 2020 [PMID: 33257808] (Bioassay, Human) Bioassay Human
YS Hwang, S Suzuki, Y Seita, J Ito, Y Sakata, H Aso, K Sato, BP Hermann, K Sasaki Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cells Nat Commun, 2020;11(1):5656. 2020 [PMID: 33168808] (Cell Culture, Human) Cell Culture Human
EP Nora, L Caccianini, G Fudenberg, K So, V Kameswaran, A Nagle, A Uebersohn, B Hajj, AL Saux, A Coulon, LA Mirny, KS Pollard, M Dahan, BG Bruneau Molecular basis of CTCF binding polarity in genome folding Nat Commun, 2020;11(1):5612. 2020 [PMID: 33154377] (Bioassay, Mouse) Bioassay Mouse
Show All 400 Publications.

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Bioinformatics

Gene Symbol BMP4
Uniprot