Sirtuin 1/SIRT1 Antibody (1F3)

Images

 
Western Blot: SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.
Immunocytochemistry/ Immunofluorescence: SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.
Flow Cytometry: SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ...read more
Immunocytochemistry/ Immunofluorescence: SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
1F3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Sirtuin 1/SIRT1 Antibody (1F3) Summary

Immunogen
Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIRT1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Jurkat Lysate (NB800-PC2)
MCF-7 Lysate (NB800-PC5)
HeLa Lysate (NB800-PC1)
Publications
Read Publication using
NBP1-51641 in the following applications:

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for Sirtuin 1/SIRT1 Antibody (1F3)

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1

Background

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Sirtuin 1/SIRT1 Antibody (NBP1-51641)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-FrFl.


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IHC-FrFl
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sirtuin 1/SIRT1 Antibody (NBP1-51641). (Showing 1 - 1 of 1 FAQ).

  1. Does this product recognize C or N-terminal fraction?
    • The immunogen consists of amino acids: 265-452, so the C-terminus of the target protein

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

PE Labeled NBP1-51641PE

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Bioinformatics Tool for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

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Bioinformatics

Gene Symbol SIRT1
Entrez
Uniprot