Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free


Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein more
Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI more
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration more
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB more
Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control more
Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).

Product Details

Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, ICC/IF, IHC, IHC-P, IHC-FrFl
BSA Free
1.0 mg/ml

Order Details

Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free Summary

Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/Immunofluorescence 1:10-1:1000
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This SIRT1 (1F3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. Use in Immunohistochemistry-free floating reported in scientific literature (PMID: 26327687).
Theoretical MW
120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Jurkat Whole Cell Lysate
HeLa Whole Cell Lysate
Read Publications using
NBP1-51641 in the following applications:

Reactivity Notes

Human, mouse, rat and monkey. Use in Rabbit reported in scientific literature (PMID:32179074).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Ammonium sulfate precipitation

Alternate Names for Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free

  • EC 3.5.1
  • S. cerevisiae, homolog) 1
  • SIR2alpha
  • SIR2L1
  • SIR2-like protein 1
  • SIRT1
  • Sirtuin 1


SIRT1, the human homolog of the S. cerevisiae SIR2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Ce
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
Species: Hu, Mu, Rt
Applications: IHC, WB
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Mu, Rt, Sq, Xp
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
Species: Gt, Ha, Hu, Mu, Po, Rt, Sh, Sq
Applications: ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC, ICC/IF, IHC-WhMt, IHC, IHC-P, WB
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, Flow, ICC/IF, WB
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
Species: Hu, Mu, Md, Pm, Rt
Applications: ChIP, EM, Flow-IC, Flow, ICC/IF, IP, In vitro, WB
Species: Hu, Mu, Rt
Applications: ELISA
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Species: Bv, Ca, Hu, Mu, Pm
Applications: WB

Publications for Sirtuin 1/SIRT1 Antibody (NBP1-51641)(8)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 3 applications: IHC-FrFl, KD, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-51641 Applications Species
Gao Y, Kamogashira T, Fujimoto C Et al. Pyrroloquinoline quinone (PQQ) protects mitochondrial function of HEI-OC1 cells under premature senescence NPJ Aging 2022-08-04 [PMID: 35927260]

Citation using the Alexa Fluor 647 version of this antibody.
Toma L, Barbalata T, Sanda G Et al. CRISPR/dCas9 Transcriptional Activation of Endogenous Apolipoprotein AI and Paraoxonase 1 in Enterocytes Alleviates Endothelial Cell Dysfunction Biomolecules 2021-11-25 [PMID: 34944413] (WB, Human) WB Human
Bansod S, Godugu C Nimbolide ameliorates pancreatic inflammation and apoptosis by modulating NF-kappa B/SIRT1 and apoptosis signaling in acute pancreatitis model International immunopharmacology 2020-12-09 [PMID: 33310297] (Mouse) Mouse
Kong H, Wang H, Zhuo Z et al. Inhibition of miR-181a-5p reduces astrocyte and microglia activation and oxidative stress by activating SIRT1 in immature rats with epilepsy Lab. Invest. 2020-05-27 [PMID: 32461588] (KD, WB, Rat) KD, WB Rat
Bansod S, Aslam Saifi M, Khurana A, Godugu C Nimbolide abrogates cerulein-induced chronic pancreatitis by modulating beta-catenin/Smad in a sirtuin-dependent way Pharmacol Res 2020-03-21 [PMID: 32194177]
Xiao Y, Zhou L, Zhang T et al. Anti-fibrosis activity of quercetin attenuates rabbit tracheal stenosis via the TGF-beta/AKT/mTOR signaling pathway Life Sci. 2020-06-01 [PMID: 32179074] (WB, Rabbit, Human) WB Rabbit, Human
Takata T, Sakasai-Sakai A, Takino JI, Takeuchi M Evidence for Toxic Advanced Glycation End-Products Generated in the Normal Rat Liver Nutrients 2019-07-16 [PMID: 31315223] (WB, Rat) WB Rat
Lo Iacono L, Visco-Comandini F, Valzania A et al. Adversity in childhood and depression: linked through SIRT1. Transl Psychiatry 2015-09-02 [PMID: 26327687] (IHC-FrFl, Mouse) IHC-FrFl Mouse

Reviews for Sirtuin 1/SIRT1 Antibody (NBP1-51641) (0)

There are no reviews for Sirtuin 1/SIRT1 Antibody (NBP1-51641). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Sirtuin 1/SIRT1 Antibody (NBP1-51641). (Showing 1 - 1 of 1 FAQs).

  1. Does this product recognize C or N-terminal fraction?
    • The immunogen consists of amino acids: 265-452, so the C-terminus of the target protein

Control Lysate(s)

Secondary Antibodies


Isotype Controls

Additional Sirtuin 1/SIRT1 Products

Bioinformatics Tool for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

Discover related pathways, diseases and genes to Sirtuin 1/SIRT1 Antibody (NBP1-51641). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

Discover more about diseases related to Sirtuin 1/SIRT1 Antibody (NBP1-51641).

Pathways for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

View related products by pathway.

PTMs for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

Learn more about PTMs related to Sirtuin 1/SIRT1 Antibody (NBP1-51641).

Research Areas for Sirtuin 1/SIRT1 Antibody (NBP1-51641)

Find related products by research area.

Blogs on Sirtuin 1/SIRT1

There are no specific blogs for Sirtuin 1/SIRT1, but you can read our latest blog posts.
Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free and receive a gift card or discount.


Gene Symbol SIRT1