Sirtuin 1/SIRT1 Antibody (1F3) [PE]

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

Sirtuin 1/SIRT1 Antibody (1F3) [PE] Summary

• Immunogen: Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:32179074).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for Sirtuin 1/SIRT1 Antibody (1F3) [PE]

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

SIRT1, the human homolog of the S. cerevisiae Sir2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT1