KAT3B/p300 Antibody (RW105) - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). ...read more
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW105) antibody NB100-616 (blue) and a matched isotype control NBP2-27287 (orange). ...read more
Flow Cytometry: KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on Raw264.7 cells with KAT3B/p300 Antibody (RW105) NB100-616 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Genetic Strategies: Knockdown Validated: KAT3B/p300 Antibody (RW105) [NB100-616] - 293T cells were treated with different siRNAs against p300 alone for 2 days and core histones were prepared and subjected to WB ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Md, PmSpecies Glossary
Applications WB, Flow, Func, ICC/IF, IP, MS, ChIP, KD
Clone
RW105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

KAT3B/p300 Antibody (RW105) - BSA Free Summary

Immunogen
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Localization
Nuclear
Specificity
This is specific for p300 protein. This recognizes residues 1921-2023.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EP300
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Electron Microscopy reported in scientific literature (PMID 25728767)
  • Flow (Intracellular) 1ug/mL
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • In vitro assay reported in scientific literature (PMID 25728767)
  • Knockdown Validated Validated for Knockdown from a verified customer review.
  • Western Blot 1:250-1:500
Control
HeLa Nuclear Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NB100-616 in the following applications:

Publications
Read Publications using
NB100-616 in the following applications:

Reactivity Notes

Mink.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KAT3B/p300 Antibody (RW105) - BSA Free

  • E1A binding protein p300
  • E1A-associated protein p300
  • E1A-binding protein, 300kD
  • EC 2.3.1
  • EC 2.3.1.48
  • EP300
  • histone acetyltransferase p300
  • KAT3B
  • p300 HAT
  • p300
  • RSTS2

Background

In the eukaryotic genome, chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB2676
Species: Hu, Mu, Rt
Applications: ICC, WB
MAB5285
Species: Hu
Applications: Simple Western, WB
NBP3-09392
Species: Hu, Mu
Applications: WB
NB100-81898
Species: Hu, Po
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-76729
Species: Hu
Applications: ELISA, ICC/IF, IP, WB
H00008850-M04
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP2-01264
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP2-13943
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-29463
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-32870
Species: Ch, Hu
Applications: IHC, IHC-P, IP, KD, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NB200-310
Species: Hu, Pm, Mu, Rt
Applications: ChIP, IP, WB
H00001523-M01
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
DYC2510-2
Species: Hu, Mu, Rt
Applications: ELISA
2695-SE
Species: Hu
Applications: EnzAct
NBP2-29661
Species: Hu, Mu, Rt
Applications: ELISA

Publications for KAT3B/p300 Antibody (NB100-616)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Chemotaxis, EM, IP, In-vitro, WB.


Filter By Application
Chemotaxis
(2)
EM
(1)
IP
(2)
In-vitro
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-616 Applications Species
Qin YP, Yu HB, Yuan SY et al. KAT2A Promotes Hepatitis B Virus Transcription and Replication Through Epigenetic Regulation of cccDNA Minichromosome Frontiers in Microbiology 2022-01-24 [PMID: 35140694]
Dhar SS, Zhao D, Lin T et al. MLL4 Is Required to Maintain Broad H3K4me3 Peaks and Super-Enhancers at Tumor Suppressor Genes Mol. Cell 2018-06-07 [PMID: 29861161] (Chemotaxis, Mouse) Chemotaxis Mouse
He M, Zheng B, Zhang Y et al. KLF4 mediates the link between TGF-beta1-induced gene transcription and H3 acetylation in vascular smooth muscle cells. FASEB J 2015-09-01 [PMID: 26082460]
Yi P, Wang Z, Feng Q et al. Structure of a biologically active estrogen receptor-coactivator complex on DNA Mol. Cell 2015-03-19 [PMID: 25728767] (EM, In-vitro) EM, In-vitro
Byun SW, Chang YJ, Chung IS et al. Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter Cancer Lett 2012-08-03 [PMID: 22867947] (Chemotaxis, Human) Chemotaxis Human
Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev;13(1):64-75. 1999-01-01 [PMID: 9887100]
Nemethova M, Wintersberger E. Polyomavirus large T antigen binds the transcriptional coactivator protein p300. J Virol;73(2):1734-9. 1999-02-01 [PMID: 9882390] (WB, IP, Mouse) WB, IP Mouse
Eckner R et al. Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. Genes Dev;1 (19):2478-90. 1996-10-01 [PMID: 8843199]
Eckner R et al. Association of p300 and CBP with simian virus 40 large T antigen. Mol Cell Biol;16(7):3454-64. 1996-07-01 [PMID: 8668161] (IP, Mouse) IP Mouse
Eckner R et al. Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes Dev;8(8):869-84. 1994-04-15 [PMID: 7523245] (WB, Human) WB Human

Review for KAT3B/p300 Antibody (NB100-616) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-616:
Filter by Applications
KD
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Knockdown Validated KAT3B/p300 NB100-616
Enlarge
5
reviewed by:
Verified Customer
KD Mouse 06/23/2023
View

Summary

ApplicationKnockdown Validated
Sample TestedMouse brain
SpeciesMouse
LotE-1

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.***
WB protocol is 6% gel for running, 40V overnight transfer membrane. primary Ab 1:500, secondary Ab 1:5000.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KAT3B/p300 Antibody (NB100-616). (Showing 1 - 1 of 1 FAQs).

  1. I'm in the market for a monoclonal antibody for p300/KAT3B for WB, that would react with mouse p300. In your inventory you have some listed that I saw meet these criteria. I'm interested in knowing more about the RW antibodies: RW105, RW109 and RW128. Do you know if in addition to WB, any of these can also be used in IP or ChIP?
    • Thank you for contacting Novus Biologicals regarding our p300 antibodies. The three clones you are interested in are all guaranteed to work in mouse, and in WB and IP. We haven't yet tested these in ChIP, but if you wanted to try them and let us know how those results turn out you actually qualify for our Innovators Reward Program.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional KAT3B/p300 Products

Diseases for KAT3B/p300 Antibody (NB100-616)

Discover more about diseases related to KAT3B/p300 Antibody (NB100-616).
 

Pathways for KAT3B/p300 Antibody (NB100-616)

View related products by pathway.

PTMs for KAT3B/p300 Antibody (NB100-616)

Learn more about PTMs related to KAT3B/p300 Antibody (NB100-616).
 

Research Areas for KAT3B/p300 Antibody (NB100-616)

Find related products by research area.

Blogs on KAT3B/p300

There are no specific blogs for KAT3B/p300, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
06/23/2023
Application: KD
Species: Mouse

Bioinformatics

Gene Symbol EP300