KAT3B/p300 Antibody (RW105)

Images

 
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/ Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells ...read more
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE ...read more
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on THP-1 cells with KAT3B/p300 (RW105) antibody NB100-616 (blue) and a matched isotype control NBP2-27287 (orange). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Md, PmSpecies Glossary
Applications WB, ChIP, EM, Flow-IC, ICC/IF, In vitro, IP
Clone
RW105
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

KAT3B/p300 Antibody (RW105) Summary

Immunogen
Fusion protein containing residues 1572-2371 of human KAT3B/p300. [UniProt# Q0947]
Epitope
The epitope recognized is found between amino acids 1921-2023.
Localization
Nuclear
Specificity
This is specific for p300 protein. This recognizes residues 1921-2023.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EP300
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:250-1:500
  • Chromatin Immunoprecipitation
  • Electron Microscopy
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Flow (Intracellular)
Application Notes
This KAT3B/p300 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. Use in In-vitro and Electron Microscopy reported in scientific literature (PMID 25728767)
Positive Control
HeLa Lysate (NB800-PC9)
KAT3B/p300 Lysate (NBP2-64769)
Publications
Read Publications using
NB100-616 in the following applications:

  • 1 publication
  • EM
    1 publication
  • IP
    2 publications
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, mouse, rat, monkey, and mink.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for KAT3B/p300 Antibody (RW105)

  • E1A binding protein p300
  • E1A-associated protein p300
  • E1A-binding protein, 300kD
  • EC 2.3.1
  • EC 2.3.1.48
  • EP300
  • histone acetyltransferase p300
  • KAT3B
  • p300 HAT
  • p300
  • RSTS2

Background

In eukaryotic genome, the chromatin dynamicity is crucial for regulation of processes requiring access to genetic information and it is regulated by several different factors including post-translational modifications of histone/non-histone components, especially via acetylation process which is catalyzed by enzymes known as lysine acetyltransferases (KATs). Among different KATs, KAT3B/ p300 and its homologue KAT3A/CBP (CREB-binding protein) are extensively studied KATs with well known cellular functions. KAT3B/ p300 and KAT3A/CBP have been shown to directly interact with several different DNA-binding transcription factors which includes nuclear hormone receptors, CREB, c-Fos, C-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD. Moreover, both KAT3B/p300 and KAT3A/CBP have been shown to exert HAT (histone acetyltransferase) activity, capable of acetylating all four core histone particles in nucleosomes and to play a key role in activating chromatin for transcription. KAT3B/p300 is a potent transcriptional coactivator that has been shown to regulate various biological processes such as cellular proliferation, differentiation, cell cycle, apoptosis, and response to genetic damage, and KAT3B/ p300 dysfunction interfering with these processes implicates in the pathogenesis of several diseases such as cancer, cardiac diseases, diabetes mellitus, and HIV.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KAT3B/p300 Antibody (NB100-616)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, EM, IP, In-vitro, WB.


Filter By Application
ChIP
(1)
EM
(1)
IP
(2)
In-vitro
(1)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-616 Applications Species
He M, Zheng B, Zhang Y et al. KLF4 mediates the link between TGF-beta1-induced gene transcription and H3 acetylation in vascular smooth muscle cells. FASEB J 2015 Sep [PMID: 26082460]
Yi P, Wang Z, Feng Q et al. Structure of a biologically active estrogen receptor-coactivator complex on DNA Mol. Cell 2015 Mar 19 [PMID: 25728767] (In-vitro, EM) In-vitro, EM
Byun SW, Chang YJ, Chung IS et al. Helicobacter pylori decreases p27 expression through the delta opioid receptor-mediated inhibition of histone acetylation within the p27 promoter Cancer Lett 2012 Aug 3 [PMID: 22867947] (ChIP, Human) ChIP Human
Bhattacharya S, Michels CL, Leung MK, Arany ZP, Kung AL, Livingston DM. Functional role of p35srj, a novel p300/CBP binding protein, during transactivation by HIF-1. Genes Dev;13(1):64-75. 1999 Jan 1. [PMID: 9887100]
Nemethova M, Wintersberger E. Polyomavirus large T antigen binds the transcriptional coactivator protein p300. J Virol;73(2):1734-9. 1999 Feb. [PMID: 9882390] (WB, IP, Mouse) WB, IP Mouse
Eckner R et al. Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation. Genes Dev;1 (19):2478-90. 1996 Oct 1. [PMID: 8843199]
Eckner R et al. Association of p300 and CBP with simian virus 40 large T antigen. Mol Cell Biol;16(7):3454-64. 1996 Jul. [PMID: 8668161] (IP, Mouse) IP Mouse
Eckner R et al. Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor. Genes Dev;8(8):869-84. 1994 Apr 15. [PMID: 7523245] (WB, Human) WB Human

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Product General Protocols

View specific protocols for KAT3B/p300 Antibody (NB100-616): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for KAT3B/p300 Antibody (NB100-616). (Showing 1 - 1 of 1 FAQs).

  1. I'm in the market for a monoclonal antibody for p300/KAT3B for WB, that would react with mouse p300. In your inventory you have some listed that I saw meet these criteria. I'm interested in knowing more about the RW antibodies: RW105, RW109 and RW128. Do you know if in addition to WB, any of these can also be used in IP or ChIP?
    • Thank you for contacting Novus Biologicals regarding our p300 antibodies. The three clones you are interested in are all guaranteed to work in mouse, and in WB and IP. We haven't yet tested these in ChIP, but if you wanted to try them and let us know how those results turn out you actually qualify for our Innovators Reward Program.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-616AF405
Alexa Fluor 488 Labeled NB100-616AF488
Alexa Fluor 647 Labeled NB100-616AF647
Alexa Fluor 700 Labeled NB100-616AF700
Allophycocyanin Labeled NB100-616APC
Biotin Labeled NB100-616B
DyLight 350 Labeled NB100-616UV
DyLight 405 Labeled NB100-616V
DyLight 488 Labeled NB100-616G
DyLight 550 Labeled NB100-616R
DyLight 650 Labeled NB100-616C
DyLight 680 Labeled NB100-616FR
DyLight 755 Labeled NB100-616IR
FITC Labeled NB100-616F
HRP Labeled NB100-616H
PE Labeled NB100-616PE
PerCP Labeled NB100-616PCP

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Bioinformatics

Gene Symbol EP300
Entrez