PBEF/Visfatin/NAMPT Antibody


Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (50 ug) from HeLa, RKO, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PBEF ...read more
Immunohistochemistry-Paraffin: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). ...read more
Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit ...read more

Product Details

Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IP
1.0 mg/ml

PBEF/Visfatin/NAMPT Antibody Summary

The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human pre-B-cell colony enhancing factor 1 using the numbering given in Swiss-Prot entry P43490 (GeneID 10135).
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000
  • Immunoprecipitation 2 - 10 ug/mg lysate
  • Western Blot 1:10000 - 1:25000
Application Notes
Epitope retrieval with Citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-594 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC
  • MGC117256
  • NAmPRTase
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin


The recently discovered adipocytokine PBEF is a visceral fat specific protein which has been shown to exert insulin-mimetic effects in insulin-sensitive tissues such as liver, muscle and fat. PBEF can bind to the insulin receptor and PBEF plasma levels increase with obesity. Based on their properties, adipocytokines are likely to play critical roles in metabolic syndromes such as obesity and diabetes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBEF/Visfatin/NAMPT Antibody (NB100-594)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.

Filter By Application
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Showing Publications 1 - 9 of 9.
Publications using NB100-594 Applications Species
Yagi M, Toshima T, Amamoto R et al. Mitochondrial translation deficiency impairs NAD+ -mediated lysosomal acidification The EMBO journal 2021-02-02 [PMID: 33528041] (WB, Mouse) WB Mouse
Meier FM, Frommer KW, Peters MA et al. Visfatin/pre-B-cell colony-enhancing factor (PBEF), a proinflammatory and cell motility-changing factor in rheumatoid arthritis. J Biol Chem 2012-08-01 [PMID: 22767598]
Yang HJ, Yen MC, Lin CC et al. A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity. Exp Biol Med (Maywood) 2010-07-01 [PMID: 20558841]
Hokari F, Kawasaki E, Sakai A et al. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. J Appl Physiol 2010-08-01 [PMID: 20413424]
Costford SR, Bajpeyi S, Pasarica M et al. Skeletal muscle NAMPT is induced by exercise in humans. Am J Physiol Endocrinol Metab 2010-01-01 [PMID: 19887595]
Liu P, Li H, Cepeda J et al. Regulation of inflammatory cytokine expression in pulmonary epithelial cells by pre-B-cell colony-enhancing factor via a nonenzymatic and AP-1-dependent mechanism. J Biol Chem 2009-10-01 [PMID: 19654329]
van der Veer E, Ho C, O'Neil C et al. Extension of human cell lifespan by nicotinamide phosphoribosyltransferase. J Biol Chem 2007-04-01 [PMID: 17307730]
Tan BK, Chen J, Digby JE et al. Increased visfatin messenger ribonucleic acid and protein levels in adipose tissue and adipocytes in women with polycystic ovary syndrome: parallel increase in plasma visfatin. J Clin Endocrinol Metab 2006-12-01 [PMID: 17003086]
Ye SQ, Simon BA, Maloney JP et al. Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury. Am J Respir Crit Care Med 2005-02-01 [PMID: 15579727]

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Gene Symbol NAMPT