PBEF/Visfatin/NAMPT Antibody

Images

 
Biological Strategies: Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Detection of Human and Mouse PBEF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells ...read more
Immunohistochemistry: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Sample: FFPE section of human placental fetal membrane. Antibody: Affinity purified rabbit anti-PBEF used at a dilution of 1:5,000 (0.2ug/ml). Detection: ...read more
Biological Strategies: Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Whole cell lysate from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells.
Western Blot: PBEF/Visfatin/NAMPT Antibody [NB100-594] - Whole cell lysate from 293T cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

PBEF/Visfatin/NAMPT Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 400 and 450 of human pre-B-cell colony enhancing factor 1 using the numbering given in Swiss-Prot entry P43490 (GeneID 10135).
Predicted Species
Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAMPT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:2000-1:10000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Epitope retrieval with Citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB100-594.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PBEF/Visfatin/NAMPT Antibody

  • EC 2.4.2.12
  • MGC117256
  • NAmPRTase
  • NAMPT
  • nicotinamide phosphoribosyltransferase
  • NMPRTase
  • PBEF
  • PBEF1
  • PBEF1110035O14Rik
  • PBEF1DKFZp666B131
  • Pre-B cell-enhancing factor
  • pre-B-cell colony enhancing factor 1
  • Pre-B-cell colony-enhancing factor 1
  • VF
  • Visfatin

Background

The recently discovered adipocytokine PBEF is a visceral fat specific protein which has been shown to exert insulin-mimetic effects in insulin-sensitive tissues such as liver, muscle and fat. PBEF can bind to the insulin receptor and PBEF plasma levels increase with obesity. Based on their properties, adipocytokines are likely to play critical roles in metabolic syndromes such as obesity and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PBEF/Visfatin/NAMPT Antibody (NB100-594)(8)


Showing Publications 1 - 8 of 8.
Publications using NB100-594 Applications Species
Meier FM, Frommer KW, Peters MA et al. Visfatin/pre-B-cell colony-enhancing factor (PBEF), a proinflammatory and cell motility-changing factor in rheumatoid arthritis. J Biol Chem 2012 Aug [PMID: 22767598]
Yang HJ, Yen MC, Lin CC et al. A combination of the metabolic enzyme inhibitor APO866 and the immune adjuvant L-1-methyl tryptophan induces additive antitumor activity. Exp Biol Med (Maywood) 2010 Jul [PMID: 20558841]
Hokari F, Kawasaki E, Sakai A et al. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. J Appl Physiol 2010 Aug [PMID: 20413424]
Costford SR, Bajpeyi S, Pasarica M et al. Skeletal muscle NAMPT is induced by exercise in humans. Am J Physiol Endocrinol Metab 2010 Jan [PMID: 19887595]
Liu P, Li H, Cepeda J et al. Regulation of inflammatory cytokine expression in pulmonary epithelial cells by pre-B-cell colony-enhancing factor via a nonenzymatic and AP-1-dependent mechanism. J Biol Chem 2009 Oct [PMID: 19654329]
van der Veer E, Ho C, O'Neil C et al. Extension of human cell lifespan by nicotinamide phosphoribosyltransferase. J Biol Chem 2007 Apr [PMID: 17307730]
Tan BK, Chen J, Digby JE et al. Increased visfatin messenger ribonucleic acid and protein levels in adipose tissue and adipocytes in women with polycystic ovary syndrome: parallel increase in plasma visfatin. J Clin Endocrinol Metab 2006 Dec [PMID: 17003086]
Ye SQ, Simon BA, Maloney JP et al. Pre-B-cell colony-enhancing factor as a potential novel biomarker in acute lung injury. Am J Respir Crit Care Med 2005 Feb [PMID: 15579727]

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Bioinformatics

Gene Symbol NAMPT
Entrez
Uniprot