AMPK alpha 1 Antibody (2B7)

Images

 
Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin ...read more
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were ...read more
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Flow cytometric analysis of PC-2 cells using AMPK alpha 1 mouse mAb (green) and negative control (purple).
ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

AMPK alpha 1 Antibody (2B7) Summary

Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA1
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-22127 in the following applications:

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for AMPK alpha 1 Antibody (2B7)

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increases the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for AMPK alpha 1 Antibody (NBP2-22127)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMPK alpha 1 Antibody (NBP2-22127) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22127AF350
Alexa Fluor 405 NBP2-22127AF405
Alexa Fluor 488 NBP2-22127AF488
Alexa Fluor 532 NBP2-22127AF532
Alexa Fluor 594 NBP2-22127AF594
Alexa Fluor 647 NBP2-22127AF647
Alexa Fluor 700 NBP2-22127AF700
Alexa Fluor 750 NBP2-22127AF750
Allophycocyanin NBP2-22127APC
Allophycocyanin/Cy7 NBP2-22127APCCY7
Biotin NBP2-22127B
DyLight 350 NBP2-22127UV
DyLight 405 NBP2-22127V
DyLight 488 NBP2-22127G
DyLight 550 NBP2-22127R
DyLight 594 NBP2-22127DL594
DyLight 650 NBP2-22127C
DyLight 680 NBP2-22127FR
DyLight 755 NBP2-22127IR
FITC NBP2-22127F
HRP NBP2-22127H
Janelia Fluor 549 NBP2-22127JF549
Janelia Fluor 646 NBP2-22127JF646
PE NBP2-22127PE
PE/Atto594 NBP2-22127PEATT594
PE/Cy5.5 NBP2-22127PECY55
PE/Cy7 NBP2-22127PECY7
PerCP NBP2-22127PCP

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Blogs on AMPK alpha 1.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Bioinformatics

Gene Symbol PRKAA1
Entrez
Uniprot
COVID-19 update