AMPK alpha 1 Antibody (2B7)

Images

 
Simple Western: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Simple Western lane view shows a specific band for AMPK Alpha 1 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the ...read more
Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) ...read more
Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin ...read more
Biological Strategies: Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Ability to combat ROS varies across cell lines. Western blot analysis showing levels of AMPK and activated AMPK (T172) at 24, 48 and ...read more
Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on NIH3T3 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127 (blue) and a matched isotype control (orange). Cells were fixed ...read more
Flow Cytometry: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - An intracellular stain was performed on RH-30 cells with AMPK alpha 1 Antibody (2B7) NBP2-22127PE (blue) and a matched isotype control (orange). Cells were ...read more
ELISA: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
2B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

AMPK alpha 1 Antibody (2B7) Summary

Immunogen
Purified recombinant fragment of human AMPK alpha 1 expressed in E. coli. [UniProt# Q13131]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAA1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:50
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This AMPK alpha 1 (2B7) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-22127 in the following applications:

Reactivity Notes

Human, mouse, rat and monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A or G purified

Alternate Names for AMPK alpha 1 Antibody (2B7)

  • AMP -activate kinase alpha 1 subunit
  • AMP-activated protein kinase, catalytic, alpha-1,5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK alpha 1
  • AMPK alpha 1,5'-AMP-activated protein kinase, catalytic alpha-1 chain
  • AMPK subunit alpha-1
  • AMPK
  • AMPK1
  • AMPKa1
  • EC 2.7.11
  • EC 2.7.11.1
  • MGC33776
  • MGC57364
  • PRKAA1
  • protein kinase, AMP-activated, alpha 1 catalytic subunit

Background

AMPK alpha 1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increases the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22127
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Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for AMPK alpha 1 Antibody (NBP2-22127)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(4)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP2-22127 Applications Species
Harun-Or-Rashid M, Pappenhagen N, Zubricky R et al. MCT2 overexpression rescues metabolic vulnerability and protects retinal ganglion cells in two models of glaucoma Neurobiol. Dis. May 15 2020 [PMID: 32422282] (WB, Mouse) WB Mouse
Vila IK, Park MK, Setijono SR et al A muscle-specific UBE2O/AMPK alpha 2 axis promotes insulin resistance and metabolic syndrome in obesity JCI Insight Jul 11 2019 [PMID: 31292296] (WB, Mouse) WB Mouse
Korponay TC, Balnis J, Vincent CE et al. High CO2 downregulates skeletal muscle protein anabolism via AMPK alpha 2-mediated depressed ribosomal biogenesis Am. J. Respir. Cell Mol. Biol. Jul 2 2019 [PMID: 31264907]
Harun-Or-Rashid M, Inman DM. Reduced AMPK activation and increased HCAR activation drive anti-inflammatory response and neuroprotection in glaucoma. J Neuroinflammation. Nov 13 2018 [PMID: 30424795] (WB, Mouse) WB Mouse
Chauhan AS, Liu X, Jing J et al. STIM2 interacts with AMPk and regulates calcium-induced AMPk activation. FASEB J. Oct 18 2018 [PMID: 30335546] (ICC/IF, Human) ICC/IF Human
Dai S, Dulcey AE, Hu X et al. Methyl-B-cyclodextrin restores impaired autophagy flux in Niemann-Pick C1-deficient cells through activation of AMPK. Autophagy. 2017 Jun 14 [PMID: 28613987] (WB, Human) WB Human
Vila IK, Yao Y, Kim G et al. A UBE2O-AMPKalpha2 Axis that Promotes Tumor Initiation and Progression Offers Opportunities for Therapy. Cancer Cell. 2017 Feb 13 [PMID: 28162974] (Mouse) Mouse
Xiao Y, Kwong M, Daemen A et al. Metabolic Response to NAD Depletion across Cell Lines Is Highly Variable. PLoS ONE Oct 6 2016 [PMID: 27711204]
Chen Y, Duan Y et al. Inhibition of ERK1/2 and activation of LXR synergistically reduce atherosclerotic lesions in ApoE-deficient mice. Arterioscler Thromb Vasc Biol Jan 4 2015 [PMID: 25810299] (WB, Human) WB Human

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for AMPK alpha 1 Antibody (NBP2-22127) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22127AF350
Alexa Fluor 405 NBP2-22127AF405
Alexa Fluor 488 NBP2-22127AF488
Alexa Fluor 532 NBP2-22127AF532
Alexa Fluor 594 NBP2-22127AF594
Alexa Fluor 647 NBP2-22127AF647
Alexa Fluor 700 NBP2-22127AF700
Alexa Fluor 750 NBP2-22127AF750
Allophycocyanin NBP2-22127APC
Allophycocyanin/Cy7 NBP2-22127APCCY7
Biotin NBP2-22127B
DyLight 350 NBP2-22127UV
DyLight 405 NBP2-22127V
DyLight 488 NBP2-22127G
DyLight 550 NBP2-22127R
DyLight 594 NBP2-22127DL594
DyLight 650 NBP2-22127C
DyLight 680 NBP2-22127FR
DyLight 755 NBP2-22127IR
FITC NBP2-22127F
HRP NBP2-22127H
Janelia Fluor 549 NBP2-22127JF549
Janelia Fluor 646 NBP2-22127JF646
PE NBP2-22127PE
PE/Atto594 NBP2-22127PEATT594
PE/Cy5.5 NBP2-22127PECY55
PE/Cy7 NBP2-22127PECY7
PerCP NBP2-22127PCP

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AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

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Bioinformatics

Gene Symbol PRKAA1
Entrez
Uniprot