AMPK alpha 2 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for AMPK alpha 2 at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

AMPK alpha 2 was detected in immersion fixed HEK293 human embryonic kidney cell line using Goat Anti-Human/Mouse/Rat AMPK alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2850) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AMPK alpha 2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AMPK alpha 2
  Phe340-Arg552
  Accession # P54646
• Specificity: Detects human, mouse, and rat AMPK alpha 2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human AMPK alpha 1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRKAA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AMPK alpha 2 Antibody [Unconjugated]

• 5'-AMP-activated protein kinase catalytic subunit alpha-2
• AMPK alpha 2
• AMPK subunit alpha-2
• AMPK2
• AMPK5'-AMP-activated protein kinase, catalytic alpha-2 chain
• AMPK-alpha-2 chain
• EC 2.7.11
• EC 2.7.11.1
• PRKAA
• PRKAA2
• protein kinase, AMP-activated, alpha 2 catalytic subunit

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The catalytic alpha subunit of AMPK is activated allosterically by AMP, and by phosphorylation via the AMPK kinase LKB1. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPK alpha 2 Antibody (AF2850)(6)

Publications using AF2850

• CH Chan, SN Wu, BY Bao, HW Li, TL Lu MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake International Journal of Molecular Sciences, 2021-01-20;22(3):. 2021-01-20 [PMID: 33498219] (IHC, Mouse)
• M Domise, F Sauvé, S Didier, R Caillerez, S Bégard, S Carrier, M Colin, C Marinangel, L Buée, V Vingtdeux Neuronal AMP-activated protein kinase hyper-activation induces synaptic loss by an autophagy-mediated process Cell Death Dis, 2019-03-04;10(3):221. 2019-03-04 [PMID: 30833547] (Western Blot, Mouse)
• Manon Domise AMP-activated protein kinase modulates tau phosphorylation and tau pathology in vivo Sci Rep, 2016-05-27;6(0):26758. 2016-05-27 [PMID: 27230293] (IHC, Mouse)
• Jiang S, Park D, Stigler W, Creighton J, Ravi S, Darley-Usmar V, Zmijewski J Mitochondria and AMP-activated protein kinase-dependent mechanism of efferocytosis. J Biol Chem, 2013-07-29;288(36):26013-26. 2013-07-29 [PMID: 23897815] (Western Blot, Mouse)
• Komori T, Doi A, Nosaka T, Furuta H, Akamizu T, Kitamura T, Senba E, Morikawa Y Regulation of AMP-activated protein kinase signaling by AFF4 protein, member of AF4 (ALL1-fused gene from chromosome 4) family of transcription factors, in hypothalamic neurons. J. Biol. Chem., 2012-04-23;287(24):19985-96. 2012-04-23 [PMID: 22528490] (Western Blot, Mouse)
• Gan Z, Burkart-Hartman EM, Han DH, Finck B, Leone TC, Smith EY, Ayala JE, Holloszy J, Kelly DP The nuclear receptor PPARbeta/delta programs muscle glucose metabolism in cooperation with AMPK and MEF2. Genes Dev., 2011-12-01;25(24):2619-30. 2011-12-01 [PMID: 22135324] (ChIP, Mouse)

Bioinformatics

• Gene Symbol: PRKAA2
• Uniprot:
  • P54646()