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HDAC9 Antibody

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Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml ...read more
Immunohistochemistry-Paraffin: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-03993 (bottom left, right) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Bv, Ca, PmSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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HDAC9 Antibody Summary

Immunogen
A portion of amino acids 100-150 of human HDAC-9 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HDAC9
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP2-03993 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human, horse, rat, mouse, opossum, chimpanzee, rhesus monkey, orangutan, dog and cow and 94% homologous in chicken.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HDAC9 Antibody

  • EC 3.5.1.98
  • HD7b
  • HD7HDAC
  • HD9
  • HDAC7
  • HDAC7BDKFZp779K1053
  • HDRP
  • histone deacetylase 4/5-related protein
  • Histone deacetylase 7B
  • histone deacetylase 9
  • Histone deacetylase-related protein
  • KIAA0744HDAC9B
  • MEF-2 interacting transcription repressor (MITR) protein
  • MEF2-interacting transcription repressor MITR
  • MITRHDAC9FL

Background

HDAC-9 is a histone deacetylase with an important role in the regulation of gene expression and hematopoiesis. A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid. It helps in deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. MEF2-dependent transcription is repressed by HDAC-9 by recruiting HDAC-1 and/or HDAC-3. It also inhibits skeletal myogenesis and is involved in heart development. Ubiquitous but variable expression is seen in brain and other tissues like heart, pancreas and also in human bladder carcinoma cells. Lack of HDAC-9 protein is found in a family with Peters anomaly, a central corneal leukoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HDAC9 Antibody
NBP2-03993
HDAC9 Antibody
Species: Hu, Bv, Ca, Pm
Applications: WB, IHC, IHC-P
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Publications for HDAC9 Antibody (NBP2-03993) (0)

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Review for HDAC9 Antibody (NBP2-03993) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-03993:
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Western Blot HDAC9 NBP2-03993
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reviewed by:
Anonymous
 WB Human 07/15/2016
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Summary

ApplicationWestern Blot
Sample TestedHuman Coronary Artery Endothelial Cell lysate
SpeciesHuman
Lot08123423B

Blocking

Blocking Details5% Bovine Serum Albumen in TBS-tween 0.05%

Primary Anitbody

Dilution Ratio1:1000 in 5% BSA in TBS-tween, overnight at 4 degrees celcius

Secondary Antibody

Secondary DescriptionGoat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate
Secondary Manufacturer Cat#ThermoFisher Scientific 31460
Secondary Concentration1:15000, 1 hour at room temperature diluted in 5% Skim milk in TBS-tween

Details

Detection NotesImaged with enhanced chemiluminescence on a biorad Chemidoc touch, 10 second exposure

Product General Protocols

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FAQs for HDAC9 Antibody (NBP2-03993) (0)

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Secondary Antibodies

 

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Western Blot HDAC9 NBP2-03993
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 Anonymous
07/15/2016
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol HDAC9
Uniprot