HDAC9 Antibody - BSA Free

Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Bv, Ca, Pm, Rt, Pm, Eq, Ma-Op, Pm-Or, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HDAC9 Antibody - BSA Free Summary

• Immunogen: A portion of amino acids 100-150 of human HDAC-9 was used as the immunogen.
• Predicted Species: Rat (100%), Equine (100%), Rhesus Macaque (100%), Opossum (100%), Orangutan (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HDAC9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HDAC9 Antibody - BSA Free

• EC 3.5.1.98
• HD7b
• HD7HDAC
• HD9
• HDAC7
• HDAC7B
• HDAC7BDKFZp779K1053
• HDAC9
• HDRP
• histone deacetylase 4/5-related protein
• Histone deacetylase 7B
• histone deacetylase 9
• Histone deacetylase-related protein
• KIAA0744
• KIAA0744HDAC9B
• MEF-2 interacting transcription repressor (MITR) protein
• MEF2-interacting transcription repressor MITR
• MITR
• MITRHDAC9FL

Background

HDAC-9 is a histone deacetylase with an important role in the regulation of gene expression and hematopoiesis. A nuclear protein, HDAC-9 belongs to the histone deacetylase family with several isoforms and is inhibited by Trichostatin A (TSA) and suberoylanilide hydroxamic acid. It helps in deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) and gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. MEF2-dependent transcription is repressed by HDAC-9 by recruiting HDAC-1 and/or HDAC-3. It also inhibits skeletal myogenesis and is involved in heart development. Ubiquitous but variable expression is seen in brain and other tissues like heart, pancreas and also in human bladder carcinoma cells. Lack of HDAC-9 protein is found in a family with Peters anomaly, a central corneal leukoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for HDAC9 Antibody (NBP2-03993) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Laura Lecce WB Human 07/15/2016

Summary

• Application: Western Blot
• Sample Tested: Human Coronary Artery Endothelial Cell lysate
• Species: Human
• Lot: 08123423B

Bioinformatics

• Gene Symbol: HDAC9
• Uniprot:
  • AAI11736.1(Human)