PPAR gamma/NR1C3 Antibody

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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% ...read more
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

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PPAR gamma/NR1C3 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human PPAR gamma protein (between residues 20-120) [UniProt P37231]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This PPAR gamma antibody is useful for Immunohistochemistry paraffin embedded sections and Western blot, where a band is detected at ~55 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Adrenal Lysate (NB820-59171)
Adipose Lysate (NB820-59170)
PPAR gamma/NR1C3 Lysate (NBP2-65304)
PPAR gamma/NR1C3 Lysate (NBP2-65366)
Reviewed Applications
Read 1 Review rated 3
using
NBP2-22106 in the following applications:

Publications
Read Publications using
NBP2-22106 in the following applications:

  • WB
    3 publications

Reactivity Notes

Immunogen has 87% homology to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR gamma/NR1C3 Antibody

  • CIMT1
  • NR1C3
  • NR1C3GLM1
  • Nuclear receptor subfamily 1 group C member 3
  • peroxisome proliferator-activated receptor gamma 1
  • peroxisome proliferator-activated receptor gamma
  • PPAR gamma
  • PPARG
  • PPARG1peroxisome proliferative activated receptor, gamma
  • PPARG2PPARgamma
  • PPAR-gamma

Background

Peroxisome proliferator-activated receptor gamma (PPARG), also known as nuclear receptor subfamily 1 group C member 3, NR1C3, GLM1, CIMT1, PPARG1, PPARG2, and PPARgamma, regulates adipocyte differentiation and binds peroxisome proliferators that include hypolipidemic drugs and fatty acids. PPARG regulates the peroxisomal beta-oxidation pathway of these fatty acids. PPARG activates transcription for acyl-CoA oxidase and regulates glucose homeostasis. PPARG is a member of the PPAR family, which is a subfamily of nuclear receptors. PPARG is found in the nucleus and the cytoplasm and is most prevalent in adipose tissue but also found in skeletal muscle, spleen, heart, liver, placenta, lung, and ovary. PPARG is found in three known isoforms (57.6, 54.8, and 21.6 kDa), which are produced by alternative splicing. The PPARG protein is subject to phosphorylation. PPARG has been linked to many diseases such as obesity, diabetes, atherosclerosis, and cancer. Recent research has indicated that mutations in PPARG play a role in non-alcoholic fatty liver disease (PMID: 22160635). Other research has found that using PPARG ligands as part of a chemotherapy treatment could help improve treatment for advanced pancreatic cancer (PMID: 22020928). Similar cancer research discovered that PPARG can be used to induce colorectal carcinoma cell death, making PPARG, in conjunction with survivin and caspase-3, a potential cancer cell regulator (PMID: 21765467).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR gamma/NR1C3 Antibody (NBP2-22106)(3)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 1 application: WB.


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WB
(3)
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Showing Publications 1 - 3 of 3.
Publications using NBP2-22106 Applications Species
Miller WR. PPARgamma agonism attenuates cocaine seeking behavior via association with ERk MAPk Thesis (WB, Rat)

Details:
This reference used the HRP version of NBP2-22106.
WB Rat
Miller WR. PPARgamma agonism attenuates cocaine seeking behavior via association with ERk MAPk. Thesis. Jul 6 (WB, Rat) WB Rat
Fidoamore A, Cristiano L, Laezza C et al. Energy metabolism in glioblastoma stem cells: PPARa a metabolic adaptor to intratumoral microenvironment Oncotarget 2017 Dec 12 [PMID: 29312541] (WB, Human) WB Human

Review for PPAR gamma/NR1C3 Antibody (NBP2-22106) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-22106:
Filter by Applications
WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot PPAR gamma/NR1C3 NBP2-22106
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reviewed by:
Anonymous
WB Mouse 03/27/2018
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Summary

ApplicationWestern Blot
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse
LotA-3

Comments

CommentsWestern Blot: PPAR gamma/NR1C3 Antibody [NBP2-422106SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk.

Product General Protocols

View specific protocols for PPAR gamma/NR1C3 Antibody (NBP2-22106): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPAR gamma/NR1C3 Antibody (NBP2-22106) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

HRP Labeled NBP2-22106H

Additional Array Products

Bioinformatics Tool for PPAR gamma/NR1C3 Antibody (NBP2-22106)

Discover related pathways, diseases and genes to PPAR gamma/NR1C3 Antibody (NBP2-22106). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for PPAR gamma/NR1C3 Antibody (NBP2-22106)

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Pathways for PPAR gamma/NR1C3 Antibody (NBP2-22106)

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PTMs for PPAR gamma/NR1C3 Antibody (NBP2-22106)

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Research Areas for PPAR gamma/NR1C3 Antibody (NBP2-22106)

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Blogs on PPAR gamma/NR1C3.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

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Recent Reviews

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1
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Anonymous
03/27/2018
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARG
Entrez
Uniprot