Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong ...read more
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells ...read more
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were ...read more
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with NBP1-04676 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with PGC1 alpha Antibody NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with ...read more
Product Details
Summary
Reactivity
Hu, Mu, Rt, Po, Ha, Sq, Ca, Eq, PmSpecies Glossary
Applications
WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID: 24508229). Use in IP reported in scientific literature (PMID: 24769256). Use in IHC-Frozen reported in scientific literature (PMID: 25981953). ChIP data based on customer review.
Theoretical MW
91 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Named after its association with nuclear receptor peroxisome-proliferator activated receptor (PPAR) gamma, PGC-1 alpha (PPAR gamma coactivator 1-alpha) is now known to interact with a variety of transcription factors to regulate multiple aspects of cellular physiology. PGC-1 alpha binds MYBBP1A which results in inhibition of PGC-1 alpha's transcriptional activation potential and also interact with other proteins such as PRDM16, LRPPRC, LPIN1 etc. IRSs with core sequence T(G/A)TTTT serves as PGC-1 alpha's promoter and its transcription is repressed by ZNF746. CLK2 and AMPK execute PGC-1 alpha's phosphorylation and in the skeletal muscle its phosphorylation by AMPK increases activation of its own promoter. Under the condition of high nutrients, PGC-1 alpha is heavily acetylated by GCN5 resulting in its biological inactivation whereas it gets deacetylated by SIRT1 in low nutrients/high NAD conditions. PGC-1alpha acts as transcriptional coactivator for steroid and nuclear receptors, and enhances the transcriptional activity of PPARG as well as the thyroid hormone receptor on the uncoupling protein promoter. PGC-1alpha regulates key mitochondrial genes that contribute towards adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.
I recently purchased a PGC1alpha antibody (NBP1-04676ss) for my study and was told if it worked in my animal model (Siberian Hamster) that I am eligible for a 100% credit voucher. Attached is the western blot images of white adipose tissue that was acquired using Novus' PGC1alpha antibody.
rat liver homogenates and human fibroblasts lysate
Species
Human
Lot
A-3
Comments
Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.
Blocking
Blocking Details
Left membrane: Odyssey (Li-Cor) blocking buffer. Right membrane: 5% Milk in PBS. Both 1hour at room temperature
Primary Anitbody
Dilution Ratio
dilution (1:2000) in blocking buffer (either Odyssey block buffer or PBS+5%Milk) +0.1% Tween-20, overnight incubation at 4°C
Secondary Antibody
Secondary Description
Donkey anti-rabbit IRDye 680, Odyssey Li-Cor
Secondary Manufacturer Cat#
926-32223
Secondary Concentration
1:10000
Details
Detection Notes
Image analysis with a scanner from Odyssey near infrared technology. Scanner intensity: 5
Comments
Comments
Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.
Product General Protocols
View specific protocols for PGC1 alpha Antibody (NBP1-04676):
FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 5 of 5 FAQs).
Please differentiate me between PPAR and PGC clearly.i am confuse to know the differ these two. waiting for your reply
Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product?
NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.
What is the percent homology between the human sequence used as the immunogen and the corresponding region in mouse?
The immunogen for NBP1-04676 shares over 92% homology with mouse and is guaranteed to cross-react. We have IHC data from internal testing on mouse tissues, as well as Western blot data from multiple publications.
What is the second band in the Image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary.
Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
According to UniProt, there are no methylated a.a. reported for this protein target. However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen. Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
Bioinformatics Tool for PGC1 alpha Antibody (NBP1-04676)
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Diseases for PGC1 alpha Antibody (NBP1-04676)
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![Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PGC1-alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Western-Blot-NBP1-04676-img0012.jpg "Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PGC1-alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-04676-img0008.jpg "Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.")
![Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-04676-img0011.jpg "Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.")
![Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue. Image from verified customer review.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Western-Blot-NBP1-04676-img0013.jpg "Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-PGC1 alpha at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04676-img0017.jpg "Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-PGC1 alpha at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-PGC1 alpha at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04676-img0018.jpg "Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - PC12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-PGC1 alpha at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-04676-img0010.jpg "Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with NBP1-04676 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Flow-(Intracellular)-NBP1-04676-img0016.jpg "Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with NBP1-04676 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).")
![Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with PGC1 alpha Antibody NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/PGC1-alpha-Antibody-Flow-(Intracellular)-NBP1-04676-img0014.jpg "Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with PGC1 alpha Antibody NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
![Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.](http://images.novusbio.com/fullsize2/PPAR-gamma-NR1C3-Antibody-Western-Blot-NBP2-22106-img0003.jpg)
![Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.](http://images.novusbio.com/fullsize2/PPAR-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-22106-img0001.jpg)
![Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.](http://images.novusbio.com/fullsize2/PPAR-gamma-Antibody-Western-Blot-NBP2-22106-img0002.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0004.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0008.jpg)
![Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Western-Blot-NB300-537-img0006.jpg)
![Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize2/PPAR-alpha-NR1C1-Antibody-(3B6-PPAR)-Flow-Cytometry-NB300-537-img0009.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.](http://images.novusbio.com/fullsize2/mtTFA-Antibody-(18G102B2E11)-Western-Blot-NBP1-71648-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.](http://images.novusbio.com/fullsize2/mtTFA-Antibody-(18G102B2E11)-Immunocytochemistry-Immunofluorescence-NBP1-71648-img0003.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/mtTFA-Antibody-(18G102B2E11)-Immunohistochemistry-Paraffin-NBP1-71648-img0007.jpg)
![Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extractB. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract10 % SDS-PAGE NRF1 antibody dilution: 1:1000](http://images.novusbio.com/fullsize2/Nrf1-Antibody-Western-Blot-NBP2-19586-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Nrf1 Antibody [NBP2-19586] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NRF1 protein stained by NRF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize2/Nrf1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19586-img0005.jpg)
![Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize2/Nrf1-Antibody-Immunohistochemistry-Paraffin-NBP2-19586-img0010.jpg)
![Western Blot: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Western blot analysis using AMPK alpha 1 mouse mAb against Jurkat (1), Hela (2), HepG2 (3), MCF-7 (4), Cos7 (5), NIH/3T3 (6), K562 (7), HEK293 (8), and PC-12 (9) cell lysate.](http://images.novusbio.com/fullsize2/AMPK alpha 1 Antibody (2B7)-Western Blot-NBP2-22127-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunofluorescence analysis of NTERA-2 cells using AMPK alpha 1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/AMPK alpha 1 Antibody (2B7)-Immunocytochemistry-Immunofluorescence-NBP2-22127-img0004.jpg)
![Immunohistochemistry-Paraffin: AMPK alpha 1 Antibody (2B7) [NBP2-22127] - Immunohistochemical analysis of paraffin-embedded ovarian cancer (left) and brain tissues (right) using AMPK alpha 1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/AMPK alpha 1 Antibody (2B7)-Immunohistochemistry-Paraffin-NBP2-22127-img0005.jpg)
![Western Blot: AMPK beta 1 Antibody (2C3) [H00005564-M02] - PRKAB1 monoclonal antibody (M02), clone 2C3 Analysis of PRKAB1 expression in HeLa.](http://images.novusbio.com/fullsize2/AMPK beta 1 Antibody (2C3)-Western Blot-H00005564-M02-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Analysis of monoclonal antibody to PRKAB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/AMPK-beta-1-Antibody-(2C3)-Immunocytochemistry-Immunofluorescence-H00005564-M02-img0005.jpg)
![Sandwich ELISA: AMPK beta 1 Antibody (2C3) [H00005564-M02] - Detection limit for recombinant GST tagged PRKAB1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/AMPK-beta-1-Antibody-(2C3)-Sandwich-ELISA-H00005564-M02-img0006.jpg)
![Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates](http://images.novusbio.com/fullsize2/ERR-alpha-NR3B1-Antibody-Western-Blot-NBP1-47254-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize2/ERR-alpha-NR3B1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-47254-img0007.jpg)
![Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha antibody. Some cells of tubules/ducts in the tested renal tissue depicted intense nuclear staining with an overall weak/diffused cytoplasmic staining. (10X Magnification)](http://images.novusbio.com/fullsize2/ERR-alpha-NR3B1-Antibody-Immunohistochemistry-Paraffin-NBP1-47254-img0010.jpg)
![Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.](http://images.novusbio.com/fullsize2/Glut4-Antibody-Western-Blot-NBP1-49533-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-GLUT4 [NBP1-49533] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/Glut4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49533-img0011.jpg)
![Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney](http://images.novusbio.com/fullsize2/Glut4-Antibody-Immunohistochemistry-NBP1-49533-img0005.jpg)
![Western Blot: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Analysis in control (vector only transfected HEK293T lysate) and PGC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize2/Pepsinogen-C-PGC-Progastricsin-Antibody-Western-Blot-NBP1-91011-img0013.jpg)
![Immunohistochemistry-Paraffin: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize2/Pepsinogen-C-PGC-Progastricsin-Antibody-Immunohistochemistry-Paraffin-NBP1-91011-img0019.jpg)
![Western Blot: Pepsinogen C/PGC/Progastricsin Antibody [NBP1-91011] - Analysis in human stomach tissue.](http://images.novusbio.com/fullsize2/Pepsinogen-C-PGC-Progastricsin-Antibody-Western-Blot-NBP1-91011-img0009.jpg)
![Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [1761].](http://images.novusbio.com/fullsize2/CS-Citrate-Synthase-Antibody-(1761)-Western-Blot-NBP2-43648-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Citrate synthetase protein stained by Citrate synthetase antibody [1761] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/CS-Citrate-Synthase-Antibody-(1761)-Immunocytochemistry-Immunofluorescence-NBP2-43648-img0012.jpg)
![Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.](http://images.novusbio.com/fullsize2/CS-Citrate-Synthase-Antibody-(1761)-Immunohistochemistry-Paraffin-NBP2-43648-img0004.jpg)
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Western-Blot-NBP2-31376-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Immunocytochemistry-Immunofluorescence-NBP2-31376-img0009.jpg)
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Immunohistochemistry-Paraffin-NBP2-31376-img0011.jpg)
![Western Blot: UCP2 Antibody [NB100-59742] - Staining of rat adipose (A) and mouse spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence. Approx 37 kDa band observed in rat adipose and 28-30 kDa in mouse spleen lysates (calculated MW of 33.4kDa according to Mouse NP_035801.3 and 33.4kDa according to Rat NP_062227.2).](http://images.novusbio.com/fullsize2/UCP2-Antibody-Western-Blot-NB100-59742-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: UCP2 Antibody [NB100-59742] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize2/UCP2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-59742-img0002.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")