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PGC1 alpha Antibody - BSA Free

Images

 
Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following publication ...read more
HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-PGC1 alpha Antibody NBP1-04676 at 2 ug/ml overnight at 4C and ...read more
Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in ...read more
An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells ...read more
Biological Strategies: Vehicle or RU 486 (5 mg/kg) injected mice were presented with/without corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator ...read more
Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.
HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight ...read more
Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.
An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an ...read more
An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody ...read more
Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane was probed with 1:1000 of Rabbit Polyclonal PGC1 alpha Antibody (Catalog # ...read more
DM1-derived fibroblasts have no changes in mitochondria biogenesis. (A, B) Representative images of immunofluorescence of TOMM20, and PGC1-alpha in DM1 and control fibroblasts (n=3). (C) Medium fluorescence intensity ...read more
Feeding the diabetes mellitus (DM) mice with the RSV-enriched diet improved mitochondrial biogenesis in the quadriceps muscle. A) Representative western blots using antibodies against nuclear respiratory factor (NRF-1), ...read more
Mitochondrial content and biogenesis. (d) Immunoblot analysis of PGC1alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28970491), ...read more
Atg7 deficiency in vascular smooth muscle cells (VSMCs) alters mitochondrial quality control and enhances apoptosis. c) Western blot analyses of the expression of TFEB and PGC-1-alpha proteins in aortic VSMCs isolated ...read more
At the time of second passage, vessels in successfully growing xenografts of four different tumor types surveyed were of murine origin: A-C Colon; D-F Lung; G-I Pancreatic; J-L Renal Cell Carcinoma. For each tumor type, ...read more
Knockdown of autophagy-related gene 5 (ATG5) inhibits trehalose-induced autophagy in normal human primary airway epithelial cells.Normal human tracheobronchial epithelial cells were transfected with Naito1 chimera RNAi ...read more
Expression of ER stress associated protein and autophagy related protein in the C28I2 chondrocyte infected with Ad-ATG5 or Ad-ATG7. a The ATG5 and ATG7 mRNA level were analysed by RT-PCR in C28I2 cells. b Analysis of ...read more
A431 human skin carcinoma cell line was stained with Rabbit anti-PGC1 alpha Affinity-purified Polyclonal Antibody conjugated to Phycoerythrin (Catalog # NBP1-04676PE, blue histogram) or matched control antibody (Catalog ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, PmSpecies Glossary
Applications WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

PGC1 alpha Antibody - BSA Free Summary

Immunogen
This PGC1 alpha Antibody was developed against a recombinant protein made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
Localization
Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
Predicted Species
Equine (94%), Primate (98%), Canine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARGC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported by customer review
  • Flow (Intracellular) 1 - 2.5 ug/ml
  • Flow Cytometry 1 - 2.5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 1:1000. Use reported in scientific literature (PMID 24508229)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 25981953)
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation reported in scientific literature (PMID 24769256)
  • Knockdown Validated reported in scientific literature (PMID 35455432)
  • Knockout Validated
  • Western Blot 1 - 2 ug/ml
Application Notes
In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 11 Reviews rated 4.6
using
NBP1-04676 in the following applications:

Publications
Read Publications using
NBP1-04676 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35174626, 34573421). Use in Mouse reported in scientific literature (PMID:33719499). Use in Sheep reported in scientific literature (PMID:32403966). Expected from sequence similarity: Mouse

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody - BSA Free

  • LEM6
  • Ligand effect modulator 6
  • ligand effect modulator-6
  • L-PGC-1alpha
  • peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
  • peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
  • PGC1 alpha
  • PGC1
  • PGC-1(alpha)
  • PGC1A
  • PGC-1-alpha
  • PGC1APGC-1(alpha)
  • PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
  • PGC-1v
  • PPAR gamma coactivator variant form
  • PPAR gamma coactivator-1
  • PPARgamma coactivator 1alpha
  • PPAR-gamma coactivator 1-alpha
  • PPARGC1
  • PPARGC1A
  • PPARGC-1-alpha

Background

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-04676
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Applications: WB, ChIP, Flow, ICC/IF, IHC, IP, ChIP, KD, KO

Publications for PGC1 alpha Antibody (NBP1-04676)(261)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Chicken, Goat, Hamster, Porcine, Sheep, Squirrel, Zebrafish.

We have publications tested in 14 applications: B/N, ChIP, Chemotaxis, FLOW, IB, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, IP, KD, Simple Western, WB.


Filter By Application
B/N
(6)
ChIP
(2)
Chemotaxis
(5)
FLOW
(4)
IB
(1)
ICC/IF
(11)
IF/IHC
(10)
IHC
(3)
IHC-Fr
(3)
IHC-P
(4)
IP
(4)
KD
(1)
Simple Western
(2)
WB
(175)
All Applications
Filter By Species
Human
(62)
Mouse
(99)
Rat
(29)
Chicken
(1)
Goat
(1)
Hamster
(1)
Porcine
(1)
Sheep
(1)
Squirrel
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 261. Show All 261 Publications.
Publications using NBP1-04676 Applications Species
Aimaretti E, Chimienti G, Rubeo C et al. Different Effects of High-Fat/High-Sucrose and High-Fructose Diets on Advanced Glycation End-Product Accumulation and on Mitochondrial Involvement in Heart and Skeletal Muscle in Mice Nutrients 2023-11-22 [PMID: 38068732] (WB, Mouse) WB Mouse
Xue J, Zhang J, Zhang J et al. The Parkinson's disease-associated mutation LRRK2 G2385R alters mitochondrial biogenesis via the PGC-1?-TFAM pathway Mitochondrion 2023-09-13 [PMID: 37708949] (WB, Human) WB Human
Fujiwara T, Takeda N, Hara H et al. PGC-1?-mediated angiogenesis prevents pulmonary hypertension in mice JCI insight 2023-09-08 [PMID: 37681410] (WB, IHC-P, Mouse) WB, IHC-P Mouse
Soler Palacios B, Villares R, Lucas P et al. Growth hormone remodels the 3D-structure of the mitochondria of inflammatory macrophages and promotes metabolic reprogramming Frontiers in Immunology 2023-07-05 [PMID: 37475858] (IHC) IHC
Tamiya H, Urushihara N, Shizuma K et al. SHARPIN Enhances Ferroptosis in Synovial Sarcoma Cells via NF-?B- and PRMT5-Mediated PGC1? Reduction Cancers (Basel) 2023-07-04 [PMID: 37444594]
Li C, Han S, Zhu J, Cheng F MiR-132-3p activation aggravates renal ischemia-reperfusion injury by targeting Sirt1/PGC1alpha axis Cellular signalling 2023-07-09 [PMID: 37433399] (WB, Mouse)

Details:
1:1000 WB dilution
WB Mouse
Wang H, Chen R, Xiao L et al. Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PINK1-deficient Human Dopamine Neurons bioRxiv 2023-06-30 [PMID: 37425943] (B/N, WB) B/N, WB
Vámos A, Arianti R, Vinnai B et al. Human abdominal subcutaneous-derived active beige adipocytes carrying FTO rs1421085 obesity-risk alleles exert lower thermogenic capacity Frontiers in Cell and Developmental Biology 2023-06-14 [PMID: 37416800] (WB, Human) WB Human
Pak M, Kim Y, Kim H et al. Anti-inflammatory effects of the human milk oligosaccharide, 2’-fucosyllactose, exerted via modulation of M2 microglial activation in a mouse model of ischemia-reperfusion injury Preprints 2023-05-18 [PMID: 37372011] (WB, Mouse)

Details:
1:1000 WB dilution
WB Mouse
Han J, Li S, Wang W et al. SIRT1 Activator E1231 Alleviates Nonalcoholic Fatty Liver Disease by Regulating Lipid Metabolism Current issues in molecular biology 2023-06-08 [PMID: 37367070] (WB) WB
Show All 261 Publications.

Reviews for PGC1 alpha Antibody (NBP1-04676) (11) 4.611

Average Rating: 4.6
(Based on 11 reviews)
We have 11 reviews tested in 6 species: Human, Mouse, Rat, Human and Mouse, Mouse and mus musculus, Other.

Reviews using NBP1-04676:
Filter by Applications
WB
(10)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rat
(3)
Human and Mouse
(1)
Mouse and mus musculus
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PGC1 alpha NBP1-04676
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5
reviewed by:
Robin Ren
WB Human and Mouse 10/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate, Sample Amount: 50ug-100ug protein
SpeciesHuman and Mouse
LotG-8
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Human 12/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
LotF-1

Comments

CommentsAB DILUTIION: 1/1000
Western Blot PGC1 alpha NBP1-04676
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotG-1

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Francesca Pacifici
WB Mouse 09/26/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotF-2

Comments

CommentsWestern Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.
  5
reviewed by:
Verified Customer
ChIP Rat 04/20/2017
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedAdult spinal cord
SpeciesRat
LotE-1
  4
reviewed by:
Harini Sampath
WB Mouse and mus musculus 04/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse and mus musculus
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Green Waggener
WB Mouse 03/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotG1

Comments

CommentsC57BL/6 Mice
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Rat 11/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat Liver Nuclear Protein Extract
SpeciesRat
LotD-1
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Rat 09/16/2015
View

Summary

ApplicationWestern Blot
Sample Testedrat spinal cord
SpeciesRat
LotD-1
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Other 04/08/2015
View

Summary

ApplicationWestern Blot
SpeciesOther
Western Blot PGC1 alpha NBP1-04676
Enlarge
3
reviewed by:
Giulia Ambrosi
WB Human 04/23/2013
View

Summary

ApplicationWestern Blot
Sample Testedrat liver homogenates and human fibroblasts lysate
SpeciesHuman
LotA-3

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. What is the second band in the image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
    • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50 kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary
  3. Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
    • According to UniProt, there are no methylated a.a. reported for this protein target.  However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen.  Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
  4. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product? 
    • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

4.6
5
8
4
2
3
1
2
0
1
0

Robin Ren
10/30/2018
Application: WB
Species: Human and Mouse

Verified Customer
12/14/2017
Application: WB
Species: Human

Verified Customer
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARGC1A
Entrez
Uniprot