PGC1 alpha Antibody - BSA Free

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-12662-3), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-PGC1 alpha Antibody NBP1-04676 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.

Flow Cytometry: PGC1 alpha Antibody - BSA Free [NBP1-04676] - An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Vehicle or RU 486 (5 mg/kg) injected mice were presented with/without corticosterone (10 mg/kg) for 3 days. The expressions of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1 alpha NBP1-04676) and NIX (BNIP3L NBP1-88558) were visualized via western blotting. Loading control is beta-actin. n = 5. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33473105/) licensed under a CC-BY licence.

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL PGC1 alpha Antibody (Molecular weight: 91 KDa) in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

Western Blot: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody - BSA Free [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.

Flow (Intracellular): PGC1 alpha Antibody - BSA Free [NBP1-04676] - An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Flow (Intracellular): PGC1 alpha Antibody - BSA Free [NBP1-04676] - An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Knockout Validated: PGC1 alpha Antibody - BSA Free [NBP1-04676] - Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane was probed with 1:1000 of Rabbit Polyclonal PGC1 alpha Antibody (Catalog # NBP1-04676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PGC1 alpha at approximately 105 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.

• Reactivity: Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, Pm
• Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PGC1 alpha Antibody - BSA Free Summary

• Immunogen: This PGC1 alpha Antibody was developed against a recombinant protein made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
• Localization: Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
• Predicted Species: Equine (94%), Primate (98%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARGC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow (Intracellular) 1 - 2.5 ug/ml
  • Flow Cytometry 1 - 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1 - 2 ug/ml
• Application Notes: Use in KD reported in scientific literature (PMID:35455432) In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID: 24508229). Use in IP reported in scientific literature (PMID: 24769256). Use in IHC-Frozen reported in scientific literature (PMID: 25981953). ChIP data based on customer review..
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35174626).Use in Rat reported in scientific literature (PMID:34573421). Use in Mouse reported in scientific literature (PMID:33719499). Expected from sequence similarity: Mouse Use in Sheep reported in scientific literature (PMID:32403966).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody - BSA Free

• LEM6
• Ligand effect modulator 6
• ligand effect modulator-6
• L-PGC-1alpha
• peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
• peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• PGC1 alpha
• PGC1
• PGC-1(alpha)
• PGC1A
• PGC-1-alpha
• PGC1APGC-1(alpha)
• PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
• PGC-1v
• PPAR gamma coactivator variant form
• PPAR gamma coactivator-1
• PPARgamma coactivator 1alpha
• PPAR-gamma coactivator 1-alpha
• PPARGC1
• PPARGC1A
• PPARGC-1-alpha

Background

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGC1 alpha Antibody (NBP1-04676)(220)

Publications using NBP1-04676

• Sun X, Kato H, Sato H et al. Dopamine related oxidative stress and mitochondrial dysfunction in dopaminergic neurons differentiated from deciduous teeth derived stem cells of children with Down syndrome FASEB BioAdvances 2022-04-08 [PMID: 35812076] (WB, Human)
• Han B, Zhao H, Gong X et al. Upregulation of PGC-1 alpha Attenuates Oxygen-Glucose Deprivation-Induced Hippocampal Neuronal Injury Neural plasticity 2022-06-09 [PMID: 35719138] (Chemotaxis, Mouse)
• Yang X, Zhang M, Xie B et al. Myocardial brain-derived neurotrophic factor regulates cardiac bioenergetics through the transcription factor Yin Yang 1 Cardiovascular research 2022-06-15 [PMID: 35704040]
• Brown AD, Fogarty MJ, Davis LA et al. Mitochondrial Adaptations to Inactivity in Diaphragm Muscle Fibers Journal of applied physiology (Bethesda, Md. : 1985) 2022-06-09 [PMID: 35678745] (WB, Rat)
• Moreau, M, Capallere, C, et al. Reconstruction of functional human epidermis equivalent containing 5%IPS-derived keratinocytes treated with mitochondrial stimulating plant extracts. Sci Rep [PMID: 35641783] (Simple Western, Human)
  
  Details:
  0.180555556
• Hamuro J, Asada K, Ueno M et al. Repressed miR-34a Expression Dictates the Cell Fate to Corneal Endothelium Failure Investigative ophthalmology & visual science [PMID: 35475886] (WB, Human)
• Zhu P, Ma H, Cui S et al. ZLN005 Alleviates In Vivo and In Vitro Renal Fibrosis via PGC-1 alpha-Mediated Mitochondrial Homeostasis Pharmaceuticals (Basel, Switzerland) 2022-03-31 [PMID: 35455432] (IF/IHC, KD, WB, ICC/IF, Human, Mouse)
• O'Connor TN, Kallenbach JG, Orciuoli HM et al. Endurance exercise attenuates juvenile irradiation-induced skeletal muscle functional decline and mitochondrial stress Skeletal muscle 2022-04-12 [PMID: 35414122] (WB, Mouse)
• Cebulski K, Nowinska K, Jablonska K et al. Expression of Irisin/FNDC5 in Breast Cancer International Journal of Molecular Sciences 2022-03-24 [PMID: 35408891] (IF/IHC, Human)
• Kim YS, Yoon JW, Kim D et al. Tomatidine-stimulated maturation of human embryonic stem cell-derived cardiomyocytes for modeling mitochondrial dysfunction Experimental & molecular medicine [PMID: 35379934]
• Pirooznia SK, Wang H, Panicker N et al. Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease Science advances 2022-04-01 [PMID: 35363524] (WB, Mouse, Human)
• Takagi H, Tamura I, Fujimura T et al. Transcriptional coactivator PGC-1alpha contributes to decidualization by forming a histone-modifying complex with C/EBPbeta and p300 The Journal of biological chemistry 2022-03-28 [PMID: 35358514] (Chemotaxis, Human)
• Braga KMS, Araujo EG, Sellke FW, Abid MR Pequi Fruit Extract Increases Antioxidant Enzymes and Reduces Oxidants in Human Coronary Artery Endothelial Cells Antioxidants (Basel, Switzerland) 2022-02-28 [PMID: 35326129] (WB, Human)
• Hu L, Guo Y, Song L et al. Nicotinamide riboside promotes Mfn2-mediated mitochondrial fusion in diabetic hearts through the SIRT1-PGC1 alpha-PPAR alpha pathway Free radical biology & medicine 2022-03-19 [PMID: 35318101] (WB, Mouse)
• Jeong E, Martina JA, Contreras PS et al. The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3 Autophagy 2022-03-01 [PMID: 35230915] (WB, Human)
• Ramasubramanian B, Griffith C, Hanson M et al. Protective Effects of Chaya against Mitochondrial and Synaptic Toxicities in the Type 2 Diabetes Mouse Model TallyHO Cells 2022-02-21 [PMID: 35203393] (IF/IHC, WB, Mouse)
• Ye P, Li W, Huang X et al. BMAL1 regulates mitochondrial homeostasis in renal ischaemia-reperfusion injury by mediating the SIRT1/PGC-1alpha axis Journal of cellular and molecular medicine 2022-02-17 [PMID: 35174626] (WB, Human, Rat)
• Veeriah V, Lee SH, Levine F Long-term oral administration of an HNF4 alpha agonist prevents weight gain and hepatic steatosis by promoting increased mitochondrial mass and function Cell death & disease 2022-01-27 [PMID: 35087037] (WB, Mouse)
• Jiang J, Chen HN, Jin P et al. Targeting PSAT1 to mitigate metastasis in tumors with p53-72Pro variant Signal transduction and targeted therapy 2023-02-15 [PMID: 36788227] (WB, Human)
• Pharoah BM, Zhang C, Khodade VS et al. Hydropersulfides (RSSH) attenuate doxorubicin-induced cardiotoxicity while boosting its anticancer action Redox biology 2023-04-01 [PMID: 36773545] (WB, Rat)
• Song Q, Chen Y, Ding Q et al. mTORC1 inhibition uncouples lipolysis and thermogenesis in white adipose tissue to contribute to alcoholic liver disease Hepatology communications 2023-03-01 [PMID: 36757400] (WB, Mouse)
• Guo Z, Wang M, Ying X et al. Caloric restriction increases the resistance of aged heart to myocardial ischemia/reperfusion injury via modulating AMPK-SIRT1-PGC1a energy metabolism pathway Scientific reports 2023-02-04 [PMID: 36739302] (WB, Mouse)
• Rambout X, Cho H, Blanc R et al. PGC-1? senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII Molecular cell 2023-01-19 [PMID: 36669479] (WB, Mouse)
• Fu X, Kimura Y, Toku Y et al. Stiffer-Matrix-Induced PGC-1 alpha Upregulation Enhanced Mitochondrial Biogenesis and Oxidative Stress Resistance in Non-small Cell Lung Cancer Cellular and Molecular Bioengineering 2022-12-02 [PMID: 36660585]
• Tolomeo M, Chimienti G, Lanza M et al. Corticostriatal Projections Relying on GABA Levels Mediate Exercise-Induced Functional Recovery in Cerebral Ischemic Mice Free radical research 2022-12-08 [PMID: 36480241]
• Chao C, a , Huang C et al. SRT1720 as an SIRT1 Activator for Alleviating Paraquat-Induced Models of Parkinson's Disease Paraquat-Induced Models of Parkinson's Disease 2022-01-01 [PMID: 36379180] (IF/IHC, Mouse)
• Yu Y, Nie J, Zhao B et al. Structure characterization and anti-fatigue activity of an acidic polysaccharide from Panax ginseng C. A. Meyer Journal of ethnopharmacology 2022-10-14 [PMID: 36244638] (WB, Mouse)
• Vijayan M, Reddy PH Reduced VDAC1, Maintained Mitochondrial Dynamics and Enhanced Mitochondrial Biogenesis in a Transgenic Tau Mouse Model of Alzheimer's Disease International journal of molecular sciences 2022-08-02 [PMID: 35955694] (WB, IF/IHC, Mouse)
  
  Details:
  WB dilution used 1:1000, IHC dilution used 1:102
• Xue R, Li S, Wei Z et al. Melatonin attenuates di-(2-ethylhexyl) phthalate-induced apoptosis of human granulosa cells by inhibiting mitochondrial fission Reproductive toxicology (Elmsford, N.Y.) 2022-08-08 [PMID: 35952901] (Chemotaxis, Human)
  
  Details:
  Dilution used for ChIP 1:100
• Wang H, Wang X, Ma L et al. PGC-1 alpha regulates mitochondrial biogenesis to ameliorate hypoxia-inhibited cementoblast mineralization Annals of the New York Academy of Sciences 2022-08-02 [PMID: 35917205]
• Sun X, Dong S, Kato H et al. Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency Antioxidants (Basel, Switzerland) 2022-07-13 [PMID: 35883852] (WB, Human)
• Wang X, Zhang X, Cao K et al. Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy Nature communications 2022-07-08 [PMID: 35803927] (IB, Mouse)
  
  Details:
  Dilution: 1:2000
• Shin J, Hong S, Choi S et al. Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner Redox Biology 2022-04-01 [PMID: 35121402] (WB, Mouse)
• Bhat N, Narayanan A, Fathzadeh M, Srivastava A Dyrk1b is a key Regulatory Kinase Integrating Fgf, Shh and mTORC1 signaling in Skeletal Muscle Development and Homeostasis bioRxiv 2020-01-01 (WB, Zebrafish)
• Matadar R Metabolism Regulates Phenotype of Inner Annulus Fibrosus Cells Within Tissue Engineered Constructs Thesis 2020-01-01
• Kim KH, Lee KA Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats International journal of molecular sciences 2022-01-05 [PMID: 35008991] (WB)
• Schank M, Zhao J, Wang L Et al. Oxidative Stress Induces Mitochondrial Compromise in CD4 T Cells From Chronically HCV-Infected Individuals Front Immunol 2021-12-27 [PMID: 34956192] (FLOW, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Kawarasaki S, Matsuo K, Kuwata H et al. Screening of flavor compounds using Ucp1-luciferase reporter beige adipocytes identified 5-methylquinoxaline as a novel UCP1-inducing compoundsss Bioscience, biotechnology, and biochemistry 2021-12-22 [PMID: 34935880]
• Morales PE, Monsalves-Alvarez M, Tadinada SM et al. Skeletal muscle type-specific mitochondrial adaptation to high-fat diet relies on differential autophagy modulation FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-10-01 [PMID: 34555201] (WB, Mouse)
• Shen W, Wan X, Hou J et al. Peroxisome proliferator-activated receptor gamma coactivator 1 alpha maintains NAD + bioavailability protecting against steatohepatitis Life Medicine 2022-08-17 (WB, Mouse)
• Raghavan S, Kundumani-Sridharan V, Kumar S et al. Thioredoxin Prevents Loss of UCP2 in Hyperoxia via MKK4-p38 MAPK- PGC1 alpha Signaling and Limits Oxygen Toxicity American journal of respiratory cell and molecular biology 2021-12-10 [PMID: 34890296]
• Yu Y, Liu H, Nie J et al. Acidic polysaccharides of Mountain Cultivated Ginseng: The potential source of anti-fatigue nutrients Journal of Functional Foods 2022-08-01 (WB, Mouse)
  
  Details:
  Dilution: 1:1000
• Awasthi S, Hindle A, Sawant NA Et al. RALBP1 in Oxidative Stress and Mitochondrial Dysfunction in Alzheimer's Disease Cells 2021-11-10 [PMID: 34831336]
• Longo M, Meroni M, Paolini E Et al. TM6SF2/PNPLA3/MBOAT7 loss-of-function genetic variants impact on NAFLD development and progression both in patients and in in vitro models Cellular and molecular gastroenterology and hepatology 2021-11-22 [PMID: 34823063] (ICC/IF, WB)
• Kshirsagar S, Sawant N, Morton H Et al. Mitophagy enhancers against phosphorylated Tau-induced mitochondrial and synaptic toxicities in Alzheimer disease Pharmacological research 2021-11-08 [PMID: 34763094] (WB, Mouse)
• Bhat N, Narayanan A, Fathzadeh M Et al. Dyrk1b promotes autophagy during skeletal muscle differentiation by upregulating 4e-bp1 Cellular signalling 2021-11-06 [PMID: 34752933] (WB)
• Kim D, Lee Y, Kim HR Et al. Hypothalamic administration of sargahydroquinoic acid elevates peripheral thermogenic signaling and ameliorates high fat diet-induced obesity through the sympathetic nervous system Scientific reports 2021-10-29 [PMID: 34716371] (WB, Mouse)
• Bucher M, Kadam L, Ahuna K, Myatt L Differences in Glycolysis and Mitochondrial Respiration between Cytotrophoblast and Syncytiotrophoblast In-Vitro: Evidence for Sexual Dimorphism International journal of molecular sciences 2021-10-08 [PMID: 34639216] (WB, Human)
• Rosa-Caldwell ME, Lim S, Haynie WS Et al. Mitochondrial aberrations during the progression of disuse atrophy differentially affect male and female mice Journal of cachexia, sarcopenia and muscle 2021-09-29 [PMID: 34585846] (WB, Mouse)
• Kasamo Y, Kikuchi K, Yamakuchi M et al. 1,5-Anhydro-D-fructose Protects against Rotenone-Induced Neuronal Damage In Vitro through Mitochondrial Biogenesis International journal of molecular sciences 2021-09-14 [PMID: 34576111]
• Chimienti G, Orlando A, Russo F et al. The Mitochondrial Trigger in an Animal Model of Nonalcoholic Fatty Liver Disease Genes 2021-09-18 [PMID: 34573421] (WB, Rat)
• Song MH, Choi SC, Noh JM et al. LEFTY-PITX2 signaling pathway is critical for generation of mature and ventricular cardiac organoids in human pluripotent stem cell-derived cardiac mesoderm cells Biomaterials 2021-09-21 [PMID: 34571434] (WB, Human)
• Abbadi D, Andrews J, Katsara O, Schneider R AUF1 Gene Transfer Increases Exercise Performance and Improves Skeletal Muscle Deficit in Adult Mice Molecular Therapy - Methods & Clinical Development 2021-07-01 [PMID: 34485607] (WB)
• Nguyen TTT, Shang E, Shu C et al. Aurora kinase A inhibition reverses the Warburg effect and elicits unique metabolic vulnerabilities in glioblastoma Nature communications 2021-09-01 [PMID: 34471141] (WB, Human)
• Godoy Pa, Mennickent D, Cuchillo-Ibanez I Et Al. Increased P2x2 receptors induced by amyloid-beta peptide participates in the neurotoxicity in alzheimer's disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021-07-31 [PMID: 34343896] (ICC/IF)
• Balatskyi VV, Vaskivskyi VO, Myronova A et al. Cardiac-specific beta-catenin deletion dysregulates energetic metabolism and mitochondrial function in perinatal cardiomyocytes Mitochondrion 2021-07-21 [PMID: 34303005] (WB)
• Jiang Q, Ji A, Li D Et Al. Mitochondria damage in ambient particulate matter induced cardiotoxicity: Roles of PPAR alpha/PGC-1 alpha signaling Environmental pollution (Barking, Essex : 1987) 2021-07-14 [PMID: 34280742]
• Jing M, Cen Y, Gao F Et Al. Nephroprotective Effects of Tetramethylpyrazine Nitrone TBN in Diabetic Kidney Disease Frontiers in pharmacology 2021-06-24 [PMID: 34248629] (WB)
• Goshovska Yv, Fedichkina Ra, Balatskyi Vv Et Al. Induction of Glutathione Synthesis Provides Cardioprotection Regulating NO, AMPK and PPARa Signaling in Ischemic Rat Hearts Life (Basel, Switzerland) 2021-06-29 [PMID: 34209822] (WB)
• Huang X, Pan L, Zuo Z et al. LINC00842 inactivates transcription co-regulator PGC-1alpha to promote pancreatic cancer malignancy through metabolic remodelling Nature communications 2021-06-22 [PMID: 34158490] (IP, WB, Human)
• Xing, Y, Wei, H Et al. Methylated Vnn1 at promoter regions induces asthma occurrence via the PI3K/Akt/NF kappa B-mediated inflammation in the IUGR mice. Biol Open 2020-01-01 [PMID: 34004666]
• Wang G, Yang Y, Ma H et al. LncRNA FENDRR Inhibits ox-LDL Induced Mitochondrial Energy Metabolism Disorder in Aortic Endothelial Cells via miR-18a-5p/PGC-1 alpha Signaling Pathway Frontiers in endocrinology 2021-04-12 [PMID: 33912133] (WB, Mouse)
• Inak G, Rybak-Wolf A, Lisowski P et al. Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome Nature communications 2021-03-26 [PMID: 33771987] (WB, Human)
• Seldeen K, Shahini A, Thiyagarajan R et al. Short term nicotinamide riboside treatment improves muscle quality and function in mice as well as increases cellular energetics and differentiating capacity of myogenic progenitors Nutrition 2021-02-01 [PMID: 33744645] (WB, Mouse)
• Horvath O, Ordog K, Bruszt K et al. Modulation of Mitochondrial Quality Control Processes by BGP-15 in Oxidative Stress Scenarios: From Cell Culture to Heart Failure Oxidative medicine and cellular longevity 2021-02-27 [PMID: 33728024] (WB, Rat)
• Li X, Lu Q, Qiu Y et al. Direct Cardiac Actions of the Sodium Glucose Co-Transporter 2 Inhibitor Empagliflozin Improve Myocardial Oxidative Phosphorylation and Attenuate Pressure-Overload Heart Failure Journal of the American Heart Association 2021-03-16 [PMID: 33719499] (WB, Mouse)
• Go, G, Lee, C S Et al. PrPC Aptamer Conjugated-Gold Nanoparticles for Targeted Delivery of Doxorubicin to Colorectal Cancer Cells. Int J Mol Sci 2021-02-17 [PMID: 33671292] (IHC-Fr, Chicken)
• Petrosino JM, Longenecker JZ, Ramkumar S et al. Paracardial fat remodeling affects systemic metabolism through alcohol dehydrogenase 1 The Journal of clinical investigation 2021-02-15 [PMID: 33586683] (IHC-P)
• Horvath O, Ordog K, Bruszt K et al. BGP-15 Protects against Heart Failure by Enhanced Mitochondrial Biogenesis and Decreased Fibrotic Remodelling in Spontaneously Hypertensive Rats Oxidative medicine and cellular longevity 2021-01-30 [PMID: 33564362] (WB, Rat)
• Kashiwagi Y, Yi H, Liu S, et al. Mitochondrial biogenesis factor PGC-1 alpha suppresses spinal morphine tolerance by reducing mitochondrial superoxide Experimental neurology 2021-01-28 [PMID: 33516729] (WB, Rat)
• Choi GE, Lee HJ, Chae CW, et al. BNIP3L/NIX-mediated mitophagy protects against glucocorticoid-induced synapse defects Nature communications 2021-01-20 [PMID: 33473105] (WB, Mouse)
• Xu SH, Fu WW, Zhang J, et al. Different fuel regulation in two types of myofiber results in different antioxidant strategies in Daurian ground squirrels (Spermophilus dauricus) during hibernation The Journal of experimental biology 2021-01-18 [PMID: 33443047]
• Bianchi A, Marchetti L, Hall Z, et al. Moderate Exercise Inhibits Age-Related Inflammation, Liver Steatosis, Senescence, and Tumorigenesis Journal of immunology (Baltimore, Md. : 1950) 2021-02-15 [PMID: 33441438] (WB, Mouse)
• Ordog K, Horvath O, Eros K, et al. Mitochondrial protective effects of PARP-inhibition in hypertension-induced myocardial remodeling and in stressed cardiomyocytes Life sciences 2021-01-06 [PMID: 33421523] (WB, Rat)
• Han SY, Song HK, Cha JJ, et al. Farnesoid X receptor (FXR) agonist ameliorates systemic insulin resistance, dysregulation of lipid metabolism, and alterations of various organs in a type 2 diabetic kidney animal model Acta diabetologica 2021-01-05 [PMID: 33399988] (WB)
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  Details:
  C2C12 cells underwent Western blot analysis.
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  Primary bronchial fibroblasts from humans were transduced with GFP and underwent immunofluorescence analysis.
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  Citation using the Alexa Fluor 647 version of this antibody.
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  Citation using the Alexa Fluor 488 version of this antibody.
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  Details:
  This citation used the Alexa Fluor 488 version of this antibody.
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  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
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  Details:
  Citation using the Biotin version of this antibody.
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  Details:
  PINK1 antibody was used for WB analysis of 10 uM carbonylcyanide m-chlorophenylhydrazone /CCCP treated SH-SY5Y cells which were expressing scrambled shRNA or PINK1shRNA namely 117-2, 118-3, and 118-4 (Fig 3a).
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• Kim B, Lee H, Kawata K, Park Jy. Exercise-mediated wall shear stress increases mitochondrial biogenesis in vascular endothelium PLoS OnE et al. 2014-11-07 [PMID: 25375175] (WB, Human)
  
  Details:
  PGC1 alpha antibody used for WB on lysates of human aortic ECs (HAECs) exposed to exercise-mimicking LSS at 20 dyne/cm2 for 48 hrs, and then, recovery (Rec) LSS at 5 dyne/cm2 was followed for another 24 hrs (Figure 1b).
• Yoshizawa Sayuri, Brown Andrew, Barchowsky Aaron, Sfeir Charles. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014-08-01 [PMID: 25158202]
• LaRocca TJ, Hearon CM, Henson GD, Seals DR. Mitochondrial quality control and age-associated arterial stiffening. Exp. Gerontol. 2014-07-14 [PMID: 25034910] (WB, Mouse)
  
  Details:
  BNIP3 antibody used for WB (at 1:1000 dilution) in excised and cleaned thoracic aortas of male C57BL6 mice (young vs old) treated or not with trehalose. BNIP3 studied as mitochondrial quality control - mitophagy mediator and WB image shown in Fig 1A.
• Roland CL, Arumugam T, Deng D et al. Cell surface lactate receptor GPR81 is crucial for cancer cell survival. Cancer Res. 2014-06-13 [PMID: 24928781] (IF/IHC, Human)
  
  Details:
  IHC performed on primary human pancreatic cancer with 1:50 dilution of rabbit anti-PGC1 alpha
• Li M, Lu Y, Hu Y et al. Salvianolic acid B protects against acute ethanol-induced liver injury through SIRT1-mediated deacetylation of p53 in rats. Toxicol. Lett. 2014-04-21 [PMID: 24769256] (IP, Human, Rat)
• Yoshizawa S, Brown A, Barchowsky A. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation. Acta Biomater 2014-02-24 [PMID: 24512978] (WB, Human)
• Guo X, Dason ES, Zanon-Moreno V et al. PGC-1 alpha Signaling Coordinates Susceptibility to Metabolic and Oxidative Injury in the Inner Retina. Am. J. Pathol. 2014-02-28 [PMID: 24508229] (WB, ICC/IF, Mouse)
• Cho YR, Lim JH, Kim MY et al. Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice. PLoS One 2014-01-02 [PMID: 24392081] (WB, Human)
• Khan RS, Dine K, Das Sarma J, Shindler KS. SIRT1 Activating compounds reduce oxidative stress mediated neuronal loss in viral induced CNS demyelinating disease. Acta Neuropathol Commun 2014-01-02 [PMID: 24383546] (WB, Mouse)
• Nahirnyj A, Livne-Bar I, Guo X, Sivak JM. ROS Detoxification and Proinflammatory Cytokines Are Linked by p38 MAPK Signaling in a Model of Mature Astrocyte Activation. PLoS One 2013-12-23 [PMID: 24376630] (WB, Rat)
• Benderro GF, Tsipis CP, Sun X et al. Increased HIF-1alpha and HIF-2alpha Accumulation, but Decreased Microvascular Density, in Chronic Hyperoxia and Hypercapnia in the Mouse Cerebral Cortex. Adv Exp Med Biol 2013-01-01 [PMID: 23852473] (WB, Mouse)
• Fedorova LV, Sodhi K, Gatto-Weis C et al. Peroxisome Proliferator-Activated Receptor delta Agonist, HPP593, Prevents Renal Necrosis under Chronic Ischemia. PLoS One 2013-05-15 [PMID: 23691217] (WB, Rat)
• Xu R, Andres-Mateos E, Mejias R et al. Hibernating squirrel muscle activates the endurance exercise pathway despite prolonged immobilization. Exp Neurol 2013-01-16 [PMID: 23333568] (WB, Squirrel)
• Lim JH, Kim EN, Kim MY et al. Age-associated molecular changes in the kidney in aged mice Oxid Med Cell Longev 2012-01-01 [PMID: 23326623] (WB, Mouse)
• Khan RS, Fonseca-Kelly Z, Callinan C et al. SIRT1 activating compounds reduce oxidative stress and prevent cell death in neuronal cells Front Cell Neurosci 2012-01-01 [PMID: 23293585] (WB, Rat)
• Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle 2012-11-15 [PMID: 23070475] (WB, Human)
• Qiao C, Ma J, Xu J et al. Drosha mediates destabilization of Lin28 mRNA targets Cell Cycle 2012-08-30 [PMID: 22935707] (WB, Human)
• Benderro GF, Sun X, Kuang Y, Lamanna JC. Decreased VEGF expression and microvascular density, but increased HIF-1 and 2alpha accumulation and EPO expression in chronic moderate hyperoxia in the mouse brain Brain Res 2012-07-20 [PMID: 22820296] (WB, Mouse)
• Sun K, Asterholm IW, Kusminski CM et al. Dichotomous effects of VEGF-A on adipose tissue dysfunction Proc Natl Acad Sci U S A 2012-03-26 [PMID: 22451920] (WB, Mouse)
• Liu W, Singh R, Choi CS et al. LDL Receptor Related Protein 6 (LRP6) regulates body fat and glucose homeostasis by modulating nutrient sensing pathways and mitochondrial energy expenditure. The Journal of Biological Chemistry. 2012-01-01 [PMID: 22232553]
• Wu Y, Zhao J, Zhao W et al. Nandrolone normalizes determinants of muscle mass and fiber type after spinal cord injury. J Neurotrauma. 2012-05-20 [PMID: 22208735] (Rat)
• Rebholz SL, Jones T, Burke KT, Jaeschke A, Tso P, D'Alessio DA, Woollett LA. Multiparity leads to obesity and inflammation in mothers and obesity in male offspring. Am J Physiol Endocrinol Metab;302(4):E449-57. 2012-02-01 [PMID: 22127227]
• Benderro GF, Lamanna JC. Hypoxia-induced angiogenesis is delayed in aging mouse brain. Brain Res. 2011-03-11 [PMID: 21402058]
• Poomthavorn P, Wong SHX, Higgins S et al. Activation of a prometastatic gene expression program in hypoxic neuroblastoma cells. Endocr Relat Cancer;16(3):991-1004. 2009-01-01 [PMID: 19423615]

Reviews for PGC1 alpha Antibody (NBP1-04676) (11)

Average Rating: 4.6

(Based on 11 reviews)

We have 11 reviews tested in 6 species: Human, Mouse, Rat, Human and Mouse, Mouse and mus musculus, Other.

reviewed by: Verified Customer WB Human and Mouse 10/30/2018

Summary

• Application: Western Blot
• Sample Tested: Cell lysate, Sample Amount: 50ug-100ug protein
• Species: Human and Mouse
• Lot: G-8

reviewed by: Prabir Chakraborty WB Human 12/14/2017

Summary

• Application: Western Blot
• Sample Tested: 3 human cancer cell lines
• Species: Human
• Lot: F-1

Comments

• Comments: AB DILUTIION: 1/1000

reviewed by: Liyong Zhang WB Mouse 12/08/2017

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: G-1

Comments

• Comments: mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

reviewed by: Verified Customer WB Mouse 09/26/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle homogenate
• Species: Mouse
• Lot: F-2

Comments

• Comments: Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.

reviewed by: Hyun Yi ChIP Rat 04/20/2017

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Adult spinal cord
• Species: Rat
• Lot: E-1

reviewed by: Verified Customer WB Mouse and mus musculus 04/18/2017

Summary

• Application: Western Blot
• Sample Tested: mouse liver lysate
• Species: Mouse and mus musculus

reviewed by: Verified Customer WB Mouse 03/22/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: G1

Comments

• Comments: C57BL/6 Mice

reviewed by: Kimberly Hyatt WB Rat 11/05/2015

Summary

• Application: Western Blot
• Sample Tested: Rat Liver Nuclear Protein Extract
• Species: Rat
• Lot: D-1

reviewed by: Hyun Yi WB Rat 09/16/2015

Summary

• Application: Western Blot
• Sample Tested: rat spinal cord
• Species: Rat
• Lot: D-1

reviewed by: Bryan Tinsley WB Other 04/08/2015

Summary

• Application: Western Blot
• Species: Other

reviewed by: Verified Customer WB Human 04/23/2013

Summary

• Application: Western Blot
• Sample Tested: rat liver homogenates and human fibroblasts lysate
• Species: Human
• Lot: A-3

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
2. What is the second band in the image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
   • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50 kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary
3. Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
   • According to UniProt, there are no methylated a.a. reported for this protein target.  However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen.  Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
4. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product? 
   • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.

Bioinformatics

• Gene Symbol: PPARGC1A
• Entrez:
  • 10891(Human)
  • 19017(Mouse)
  • 83516(Rat)
• Uniprot:
  • Q9UBK2(Human)