PGC1 alpha Antibody

Knockout Validated: PGC1 alpha Antibody [NBP1-04676] - Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane was probed with 1:1000 of Rabbit Polyclonal PGC1 alpha Antibody (Catalog # NBP1-04676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for PGC1 alpha at approximately 105 kDa (as indicated) in the parental A431 cell line, but is not detectable in the knockout A431 cell line. This experiment was conducted under reducing conditions.

Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL PGC1 alpha Antibody (Molecular weight: 91 KDa) in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with PGC1 alpha Antibody [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.

Western Blot: PGC1 alpha Antibody [NBP1-04676] - Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.

Western Blot: PGC1 alpha Antibody [NBP1-04676] - Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-12662-3), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-PGC1 alpha Antibody NBP1-04676 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.

Flow Cytometry: PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

• Reactivity: Hu, Mu, Rt, Po, Gt, Ha, Sq, Ca, Eq, Pm
• Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PGC1 alpha Antibody Summary

• Immunogen: This PGC1 alpha Antibody was developed against a synthetic peptide made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
• Localization: Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
• Predicted Species: Canine (93%), Equine (94%), Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARGC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 - 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 - 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Flow (Intracellular) 1 - 2.5 ug/ml
  • Knockout Validated
• Application Notes: In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID: 24508229). Use in IP reported in scientific literature (PMID: 24769256). Use in IHC-Frozen reported in scientific literature (PMID: 25981953). ChIP data based on customer review.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  PGC1 alpha Overexpression Lysate
  Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32857105). Goat reactivity reported in scientific literature (PMID: 31158446)..

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody

• LEM6
• Ligand effect modulator 6
• ligand effect modulator-6
• L-PGC-1alpha
• peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a
• peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
• peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• PGC1 alpha
• PGC1
• PGC-1(alpha)
• PGC1A
• PGC-1-alpha
• PGC1APGC-1(alpha)
• PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
• PGC-1v
• PPAR gamma coactivator variant form
• PPAR gamma coactivator-1
• PPARgamma coactivator 1alpha
• PPAR-gamma coactivator 1-alpha
• PPARGC1
• PPARGC1A
• PPARGC-1-alpha

Background

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O.,... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGC1 alpha Antibody (NBP1-04676)(134)

Publications using NBP1-04676

• Luo X, Shi X, Sun Z et al. GCN2 Deficiency Enhances Protective Effects of Exercise on Hepatic Steatosis BioMed Research International Nov 24 2020 [PMID: 33299856] (WB, Mouse)
• Supruniuk E, Miklosz A, Chabowski A Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes Nat Commun Nov 5 2020 [PMID: 33171690] (WB, Rat)
• Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology Nov 1 2020 [PMID: 33152451] (WB, Mouse)
• Mu MD, Qian ZM, Yang SX et al. Sigma-1 receptor regulates mitophagy in dopaminergic neurons and contributes to dopaminergic protection Cell Death Dis Oct 26 2020 [PMID: 33116116]
• Mukherjee D, Chander V, Bandyopadhyay A PARIS-DJ-1 Interaction Regulates Mitochondrial Functions in Cardiomyocytes which is Critically Important in Cardiac Hypertrophy Mol Cell Biol Oct 19 2020 [PMID: 33077496]
• Subramani J, Kundumani-Sridharan V, Das KC Thioredoxin protects mitochondrial structure, function and biogenesis in myocardial ischemia-reperfusion via redox-dependent activation of AKT-CREB- PGC1 alpha pathway in aged mice Aging (Albany NY) Oct 13 2020 [PMID: 33049718] (WB, Human)
• Rosa-Caldwell ME, Lim S, Haynie WS et al. Altering aspects of mitochondrial quality to improve musculoskeletal outcomes in disuse atrophy J. Appl. Physiol. Sep 17 2020 [PMID: 32940556]
• Meyers K, Lopez M, Ho J et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice Sci Rep Sep 3 2020 [PMID: 32883997]
• Wang D, Yang Y, Zou X et al. Antioxidant Apigenin Relieves Age-Related Muscle Atrophy by Inhibiting Oxidative Stress and Hyperactive Mitophagy and Apoptosis in Skeletal Muscle of Mice J. Gerontol. A Biol. Sci. Med. Sci. Aug 28 2020 [PMID: 32857105] (WB, Mouse)
• TEglAs T, AbrahAm D, JOkai M et al. Exercise combined with a probiotics treatment alters the microbiome, but moderately affects signalling pathways in the liver of male APP/PS1 transgenic mice Biogerontology Aug 18 2020 [PMID: 32812166] (WB, Mouse)
• Kumar M, Acevedo-CintrOn J, Jhaldiyal A et al. Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PARKIN-Deficient Human Dopamine Neurons Stem Cell Reports Jul 30 2020 [PMID: 32795422] (WB, IHC, Human)
• Aljaylani A, Fluitt M, Piselli A et al. Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice Cell. Physiol. Biochem. Jul 18 2020 [PMID: 32678535]
• Wan X, Zhu X, Wang H et al. PGC1 alpha protects against hepatic steatosis and insulin resistance via enhancing IL10-mediated anti-inflammatory response FASEB J. Jul 7 2020 [PMID: 32633848]
• Chandrasekaran K, Choi J, Arvas MI et al. Nicotinamide Mononucleotide Administration Prevents Experimental Diabetes-Induced Cognitive Impairment and Loss of Hippocampal Neurons Int J Mol Sci May 26 2020 [PMID: 32466541] (WB, Rat)
• Podgorski II, Pinteric M, Marcinko D et al. Combination of sirtuin 3 and hyperoxia diminishes tumorigenic properties of MDA-MB-231 cells Life Sci. May 16 2020 [PMID: 32428596] (WB, Human)
• Harun-Or-Rashid M, Pappenhagen N, Zubricky R et al. MCT2 overexpression rescues metabolic vulnerability and protects retinal ganglion cells in two models of glaucoma Neurobiol. Dis. May 15 2020 [PMID: 32422282] (WB, Mouse)
• Bifari F, Dolci S, Bottani E et al. Complete neural stem cell (NSC) neuronal differentiation requires a branched chain amino acids-induced persistent metabolic shift towards energy metabolism Pharmacol. Res. May 12 2020 [PMID: 32407957] (WB, Human)
• Myers DA, Singleton K, Hyatt K et al. Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb Adipocyte Dec 1 2020 [PMID: 32403966] (WB, Sheep)
• Nguyen T, Zhang Y, Shang E et al. HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma models J. Clin. Invest. Apr 21 2020 [PMID: 32315286] (WB, Mouse)
• GarcIa-Puga M, Saenz-Antonanzas A et al. Myotonic Dystrophy type 1 cells display impaired metabolism and mitochondrial dysfunction that are reversed by metformin. Aging (Albany NY) Aug 4 2020 [PMID: 32310829] (ICC/IF, WB, Human)
• Arao Y, Kawai H, Kamimura K et al. Effect of methionine/choline-deficient diet and high-fat diet-induced steatohepatitis on mitochondrial homeostasis in mice Biochem. Biophys. Res. Commun. Apr 10 2020 [PMID: 32284169] (WB, Mouse)
• Xing Y, Wei H, Xiao X et al. Methylated Vnn1 at promoter regions induces asthma occurrence via the PI3K/Akt/NFkappaB-mediated inflammation in IUGR mice Biol Open Apr 28 2020 [PMID: 32139393] (WB, Mouse)
• Zheng J, Akbari M, Schirmer C et Al. Hippocampal tau oligomerization early in tau pathology coincides with a transient alteration of mitochondrial homeostasis and DNA repair in a mouse model of tauopathy Acta Neuropathol Commun Mar 4 2020 [PMID: 32131898] (WB, Human)
• Bort A, Sanchez Bg, De Miguel I et Al. Dysregulated lipid metabolism in hepatocellular carcinoma cancer stem cells Mol. Biol. Rep. Mar 3 2020 [PMID: 32125560]
• Yang Q, Sun S, Liu W et al. Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice Biochemical and Biophysical Research Communications Feb 1 2020 [PMID: 32122652] (WB, Mouse)
• Yong-SeokHan, Jun HeeLee, Sang Hun Lee Melatonin suppresses ischemia-induced fibrosis by regulating miR-149 Biochem. Biophys. Res. Commun. Feb 21 2020 [PMID: 32089265] (WB, Mouse)
• Piers TM, Cosker K, Mallach A et al. A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC-derived microglia FASEB J. Feb 1 2020 [PMID: 31907987] (WB, Human)
• Tao L, Huang X, Xu M, et al. MiR 144 protects the heart from hyperglycemia induced injury by regulating mitochondrial biogenesis and cardiomyocyte apoptosis FASEB j. Dec 12 2019 [PMID: 31907983] (WB, Mouse)
• Lu H, Ye Z, Zhai Y, et al. QKI regulates adipose tissue metabolism by acting as a brake on thermogenesis and promoting obesity EMBO Rep. Dec 23 2019 [PMID: 31868295]
• Yin J, Nielsen M, Carcione T et al. Apolipoprotein E regulates mitochondrial function through the PGC-1 alpha-sirtuin 3 pathway Aging (Albany NY) Dec 6 2019 [PMID: 31808750] (WB, Mouse)
• Langbein H, Shahid A, Hofmann A, et al. NADPH oxidase 4 mediates the protective effects of physical activity against obesity-induced vascular dysfunction Cardiovasc. Res. Dec 4 2019 [PMID: 31800011] (Mouse)
• Sun X, Seidman JS, Zhao P et al. Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic Steatohepatitis Cell Metab. Nov 14 2019 [PMID: 31761566]
• Chandrasekaran K, Salimian M, Konduru SR et al. Overexpression of Sirtuin 1 protein in neurons prevents and reverses experimental diabetic neuropathy Brain Dec 1 2019 [PMID: 31754701] (WB, Mouse)
• Gao P, Jiang Y, Wu H et al. Inhibition of Mitochondrial Calcium Overload by SIRT3 Prevents Obesity- or Age-Related Whitening of Brown Adipose Tissue Diabetes Feb 1 2020 [PMID: 31712319]
• Kim JH, Ko HY, Wang HJ et al. Effect of Dapagliflozin, a Sodium-glucose Cotransporter-2 Inhibitor, on Gluconeogenesis in Proximal Renal Tubules Diabetes Obes Metab. Nov 6 2019 [PMID: 31692240] (WB, Mouse)
• Kalvala AK, Yerra VG, Kumar A LONP1 induction by SRT1720 attenuates mitochondrial dysfunction against high glucose induced neurotoxicity in PC12 cells Toxicol In Vitro 2019 Oct 19 [PMID: 31639451] (ICC/IF, Rat)
• McGuire JL, Correll EA, Lowery AC et al. Pioglitazone improves working memory performance when administered in chronic TBI Neurobiol. Dis. Sep 9 2019 [PMID: 31513844] (WB, Rat)
• la Fuente FP, Quezada L, SepUlveda C et al. Exercise regulates lipid droplet dynamics in normal and fatty liver Biochim Biophys Acta Mol Cell Biol Lipids Aug 29 2019 [PMID: 31473346] (WB, Mouse)
• Zhou ZD, Xie SP, Saw WT et al. The Therapeutic Implications of Tea Polyphenols Against Dopamine (DA) Neuron Degeneration in Parkinson's Disease (PD) Cells Aug 16 2019 [PMID: 31426448] (WB, Human)
• Gan M, Shen L, Liu L, et al. miR-222 is involved in the regulation of genistein on skeletal muscle fiber type The Journal of Nutritional Biochemistry Dec 1 2019 (WB, Mouse)
• Suzuki J Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice Physiol Rep Jul 1 2019 [PMID: 31328438] (ICC/IF, Mouse) Effects of exercise training with short-duration intermittent hypoxia on endurance performance and muscle metabolism in well-trained mice. Physiol Rep. 2019 Jul [PMID: 31328438] (ICC/IF, Mouse)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Wang Y, Han P, Wang M et al. Artemether improves type 1 diabetic kidney disease by regulating mitochondrial function Am J Transl Res Jun 15 2019 [PMID: 31312396] (WB, Mouse)
• Hu L, Ding M, Tang D et al. Targeting mitochondrial dynamics by regulating Mfn2 for therapeutic intervention in diabetic cardiomyopathy Theranostics May 31 2019 [PMID: 31281507] (WB, ChIP, Mouse)
• Dziewulska A, Dobosz AM, Dobrzyn A et al. SCD1 regulates the AMPK/SIRT1 pathway and histone acetylation through changes in adenine nucleotide metabolism in skeletal muscle J. Cell. Physiol. Jun 26 2019 [PMID: 31241768] (Mouse)
• Brahmachari S, Lee S, Kim S, et al. Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease Brain 2019 Aug 01 [PMID: 31237944]
• Shao Y, Chen J, Freeman W, et al. Canonical Wnt Signaling Promotes Neovascularization through Determination of Endothelial Progenitor Cell Fate via Metabolic Profile Regulation Stem Cells 2019 Jun 24 [PMID: 31233254] (WB, Mouse)
• An SY, Zhang GM, Liu ZF, et al MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A Gene 2019 May 31 [PMID: 31158446] (IHC-P, WB, Goat)
• Nukaga T, Sakai D, Schol J et al. Assessment of the Main Compounds of the Lipolytic System in Treadmill Running Rats: Different Response Patterns between the Right and Left Ventricle Int J Mol Sci May 24 2019 [PMID: 31137663] (WB, Rat)
• Hemmeryckx B, Vranckx C, Bauters D Does butein affect adipogenesis? Adipocyte Dec 1 2019 [PMID: 31094273] (WB, Mouse)
• Cai YY, Zhang HB, Fan CX et al. Renoprotective effects of brown adipose tissue activation in diabetic mice J Diabetes Apr 24 2019 [PMID: 31020790] (WB, Mouse)
• Kim JS, Park JY Effects of resveratrol on laminar shear stress-induced mitochondrial biogenesis in human vascular endothelial cells J Exerc Nutrition Biochem Mar 31 2019 [PMID: 31010269] (WB, Human)
• Orlando, A;Chimienti, G;Pesce, V;Fracasso, F;Lezza, AMS;Russo, F; An In Vitro Study on Mitochondrial Compensatory Response Induced by Gliadin Peptides in Caco-2 Cells Int J Mol Sci Apr 15 2019 [PMID: 30991726] (WB, Human)
• Zheng J, Croteau DL, Bohr VA, Akbari M Diminished OPA1 expression and impaired mitochondrial morphology and homeostasis in Aprataxin-deficient cells Nucleic Acids Res. 2019 May 06 [PMID: 30986824] (WB, Human)
• Chi, L;Wang, N;Yang, W;Wang, Q;Zhao, D;Sun, T;Li, W; Protection of Myocardial Ischemia-Reperfusion by Therapeutic Hypercapnia: a Mechanism Involving Improvements in Mitochondrial Biogenesis and Function J Cardiovasc Transl Res Apr 12 2019 [PMID: 30980235] (WB, Rat)
• Nahapetyan H, Moulis M, Grousset E et al. Altered mitochondrial quality control in Atg7-deficient VSMCs promotes enhanced apoptosis and is linked to unstable atherosclerotic plaque phenotype Cell Death Dis Feb 11 2019 [PMID: 30741928] (ICC/IF, Mouse)
• Miller, CN;Samuels, JS;Azhar, Y;Parmar, A;Shashidharamurthy, R;Rayalam, S; Guggulsterone Activates Adipocyte Beiging through Direct Effects on 3T3-L1 Adipocytes and Indirect Effects Mediated through RAW264.7 Macrophages Medicines (Basel) Jan 31 2019 [PMID: 30709026] (WB, Mouse)
• Al-Horani RA, Al-Trad B, Haifawi S. Modulation of Cardiac Vascular Endothelial Growth Factor and PGC-1a with Regular Post-Exercise Cold Water Immersion of Rats J. Appl. Physiol. Jan 24 2019 [PMID: 30676864] (WB, Rat)
• Chang HW, Kim MR, Lee HJ et al. p53/BNIP3-dependent mitophagy limits glycolytic shift in radioresistant cancer. Oncogene Jan 21 2019 [PMID: 30664690] (WB, Mouse)
• Baskaran P, Covington K, Bennis J et al. Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin. Molecules Dec 4 2018 [PMID: 30518154] (WB, Mouse)
• Tao L, Wang L, Yang X et al. Recombinant human glucagon-like peptide-1 protects against chronic intermittent hypoxia by improving myocardial energy metabolism and mitochondrial biogenesis Mol. Cell. Endocrinol. Feb 5 2019 [PMID: 30503377] (WB, Mouse)
• Axelrod CL, Fealy CE, Mulya A, Kirwan JP. Exercise Training Remodels Human Skeletal Muscle Mitochondrial Fission and Fusion Machinery Towards a Pro-Elongation Phenotype. Acta Physiol (Oxf). Nov 8 2018 [PMID: 30408342] (Human)
• Liu X, Qu H, Zheng Y et al. Mitochondrial glycerol 3-phosphate dehydrogenase promotes skeletal muscle regeneration EMBO Mol Med Dec 1 2018 [PMID: 30389681] (WB, Mouse)
• Nguyen LT, Chen H, Zaky A et al. SIRT1 overexpression attenuates offspring metabolic and liver disorders as a result of maternal high-fat feeding. J. Physiol. (Lond.). Oct 31 2018 [PMID: 30381838] (WB, Mouse)
• Merz T, Wepler M, Nubbaum B et al. Cystathionine-g-lyase expression is associated with mitochondrial respiration during sepsis-induced acute kidney injury in swine with atherosclerosis. Intensive Care Med Exp. Oct 20 2018 [PMID: 30343340] (IHC, Porcine)
• Basheer WA, Fu Y, Shimura D et al. Stress response protein GJA1-20k promotes mitochondrial biogenesis, metabolic quiescence, and cardioprotection against ischemia/reperfusion injury. JCI Insight. Oct 18 2018 [PMID: 30333316] (WB, Mouse)
• Younes SA, Talla A, Pereira Ribeiro S et al. Cycling CD4+ T cells in HIV-infected immune nonresponders have mitochondrial dysfunction. J. Clin. Invest. Oct 15 2018 [PMID: 30320604] (Flow, Human)
• Arai T, Tanaka M, Goda N. HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver. Sci Rep Sep 21 2018 [PMID: 30242180] (WB, Mouse)
• Manczak M, Kandimalla R, Yin X, Hemachandra Reddy P. Mitochondrial division inhibitor 1 reduces dynamin-related protein 1 and mitochondrial fission activity. Hum. Mol. Genet. Sep 19 2018 [PMID: 30239719] (WB, Mouse)
• Niu X, Zhang J, Ni J et al. Network pharmacology-based identification of major component of Angelica sinensis and its action mechanism for the treatment of acute myocardial infarction. Biosci. Rep. Sep 19 2018 [PMID: 30232231] (WB, Rat)
• Xu J, Li Y, Lou M et al. Baicalin regulates SirT1/STAT3 pathway and restrains excessive hepatic glucose production Pharmacol. Res. Aug 22 2018 [PMID: 30144531] (WB, Human)
• Cox CS, McKay SE, Holmbeck MA et al. Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling Cell Metab. Aug 13 2018 [PMID: 30122556] (WB, Mouse)
• Finlin BS, Memetimin H, Confides AL et al. Human adipose beiging in response to cold and mirabegron JCI Insight Aug 9 2018 [PMID: 30089732] (WB, Human)
• Van Laar V S, Arnold B et al. Evidence for Compartmentalized Axonal Mitochondrial Biogenesis: Mitochondrial DNA Replication Increases in Distal Axons As an Early Response to Parkinson's Disease-Relevant Stress. J Neurosci Aug 22 2018 [PMID: 30030401] (WB, Rat)
• Hasan-Olive MM, Lauritzen kH, Ali M et al. A ketogenic Diet Improves Mitochondrial Biogenesis and Bioenergetics via the PGC1a-SIRT3-UCP2 Axis. Neurochem. Res. Jul 19 2018 [PMID: 30027365] (WB, Rat)
• kuefner MS, Deng X, Stephenson EJ et al. Secretory phospholipase A2 group IIA enhances the metabolic rate and increases glucose utilization in response to thyroid hormone. FASEB J. Jul 18 2018 [PMID: 30020829] (WB, Mouse)
• Cho JH, Kim GY, Mansfield BC, Chou JY. Sirtuin signaling controls mitochondrial function in glycogen storage disease type Ia J. Inherit. Metab. Dis. May 8 2018 [PMID: 29740774] (WB)
• Nobis S, Goichon A, Achamrah N et al. Alterations of proteome, mitochondrial dynamic and autophagy in the hypothalamus during activity-based anorexia Sci Rep May 8 2018 [PMID: 29740148] (WB, Mouse)
• Unno Y, Yamamoto H, Takatsuki S et al. Palmitoyl lactic acid induces adipogenesis and a brown fat-like phenotype in 3T3-L1 preadipocytes. Biochim. Biophys. Acta Apr 12 2018 [PMID: 29654827] (Mouse)
• Cho H, Rambout X, Gleghorn ML et al. Transcriptional coactivator PGC-1a contains a novel CBP80-binding motif that orchestrates efficient target gene expression. Genes Dev. Apr 1 2018 [PMID: 29654059] (WB, Mouse)
• Munoz VR, Gaspar RC, Kuga GK et al. Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation. J. Cell. Biochem. Mar 25 2018 [PMID: 29575149] (Mouse)
• Sun J, Chen X, Liu T et al. Berberine Protects Against Palmitate-Induced Apoptosis in Tubular Epithelial Cells by Promoting Fatty Acid Oxidation Med. Sci. Monit. Mar 12 2018 [PMID: 29528039] (WB, Human)
• Bhat N, Narayanan A, Fathzadeh M, Srivastava A Dyrk1b is a key Regulatory Kinase Integrating Fgf, Shh and mTORC1 signaling in Skeletal Muscle Development and Homeostasis bioRxiv Jan 1 2020 (WB, Zebrafish)
• Lim Ji Hee, Kim Hyung Wook, Kim Min Young et al. Cinacalcet-mediated activation of the CaMKK beta-LKB1-AMPK pathway attenuates diabetic nephropathy in db/db mice by modulation of apoptosis and autophagy. Bmc Cancer 2018 Feb 15 [PMID: 29449563] (WB, Mouse)
• Rosenkranz S, Shaposhnykov A, Trager S et al. Enhancing mitochondrial activity in neurons protects against neurodegeneration in CNS inflammation bioRxiv Jun 20 2020 (IHC-Fr, WB, Mouse)
• Wang D, Sun H, Song G et al. Resveratrol Improves Muscle Atrophy By Modulating Mitochondrial Quality Control In STZ-Induced Diabetic Mice. Mol Nutr Food Res. Mar 26 2018 [PMID: 29578301] (WB, Rat)
• Huang C, Wang K, Yang Y et al. Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation Phytomedicine 2018 Jan 01 [PMID: 29433686] (Rat)
• Ramakrishnan R, Bajbouj K, Al Heialy S et al. IL-17 Induced Autophagy Regulates Mitochondrial Dysfunction and Fibrosis in Severe Asthmatic Bronchial Fibroblasts Front. Immunol. May 21 2020 (WB, Human)
• Kim Eun Nim, Lim Ji Hee, Kim Min Young et al. Resveratrol, an Nrf2 activator, ameliorates aging-related progressive renal injury. Experimental & Molecular Medicine 2018 Jan 11 [PMID: 29326403] (WB, Mouse)
• Zhang W, Zhong W, Sun Q et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury Sci Rep 2018 Jan 11 [PMID: 29323242] (WB, Mouse)
• Bauters D, Bedossa P, Lijnen HR, Hemmeryckx B. Functional role of ADAMTS5 in adiposity and metabolic health PLoS ONE 2018 Jan 02 [PMID: 29293679] (WB, Mouse)
• Geoghegan F, Buckland RJ, Rogers E et al. Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells Oncotarget. 2017 Oct 16 [PMID: 29212260] (WB, Human)
• Chatterjee S, Daenthanasanmak A, Chakraborty P et al. CD38-NAD+Axis Regulates Immunotherapeutic Anti-Tumor T Cell Response. Cell Metab. 2018 Jan 9 [PMID: 29129787] (Flow, Mouse)
  
  Details:
  Citation used the Alexa Fluor 647 format of this antibody.
• Fakouri NB, Durhuus JA, Regnell CE et al. Rev1 contributes to proper mitochondrial function via the PARP-NAD(+)-SIRT1-PGC1a axis. Sci Rep. 2017 Oct 02 [PMID: 28970491] (WB, Mouse)
• Wang D, Chen J, Liu X et al. A Chinese herbal formula, Jian-Pi-Yi-Shen decoction, improves muscle atrophy via regulating mitochondrial quality control process in 5/6 nephrectomised rats Sci Rep 2017 Aug 23 [PMID: 28835671] (WB, Rat)
• Ryu Vitaly, Zarebidaki Eleen, Albers H Elliott et al. Short photoperiod reverses obesity in Siberian hamsters via sympathetically induced lipolysis and Browning in adipose tissue. Physiology & Behavior 2017 Jul 8 [PMID: 28694154] (WB, Hamster)
• Chung E, Joiner HE, Skelton T et al. Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts. Physiol Rep. Mar 1 2017 [PMID: 28292876] (WB, Mouse)
• Kemter E, Frohlich T, Arnold GJ et al. Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease -UMOD (ADTKD-UMOD) Sci Rep. [PMID: 28220896] (WB, Mouse)
• Lee Hun, Kim Eung Kweon, Kim Hee Young, Kim Tae-Im. Effects of Exposure to Ozone on the Ocular Surface in an Experimental Model of Allergic Conjunctivitis. PLoS One 2017 [PMID: 28046113] (WB, Mouse)
• Wang G, Song Y, Feng W et al. Activation of AMPK attenuates LPS-induced acute lung injury by upregulation of PGC1alpha and SOD1. Exp Ther Med. Sep 1 2016 [PMID: 27602077] (WB, Mouse)
• Oliviero M, Romilde I, Beatrice MM et al. Evaluations of thyme extract effects in human normal bronchial and tracheal epithelial cell lines and in human lung cancer cell line Chem. Biol. Interact. 2016 Jun 29 [PMID: 27369807] (WB, Human)
• Liu X, Wu J, Wang J et al. Possible role of mitochondrial injury in Caulis Aristolochia manshuriensis-induced chronic aristolochic acid nephropathy. Drug Chem Toxicol Jun 2 2016 [PMID: 27250112]
• Ivankovic D, Chau KY, Schapira AH, Gegg ME. Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J. Neurochem. 2015 Oct 28 [PMID: 26509433] (WB, Human)
  
  Details:
  PGC1 alpha antibody was used for WB analysis of total cell lysates of SH-SY5Y cells, TFEB-DDK cells and SH-SY5Y cells over-expressing human PGC-1a gene. WB was also performed on nuclear and cytosolic fractions of TFEB cells which were treated with 10 uM of carbonyl cyanide m- chlorophenylhydrazone(CCCP).
• De Andrade PB, Neff LA, Strosova MK et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol 2015 [PMID: 26441673]
• Kishida T, Ejima A, Yamamoto K et al. Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes. Stem Cell Reports 2015 Sep 09 [PMID: 26365511] (ICC/IF, Human)
• Liu B, Zhang R, Tao G et al. Augmented Wnt-Signaling as a Therapeutic Tool to Prevent Ischemia and Reperfusion Injury in Liver: Preclinical Studies in a Mouse Model. Liver Transpl. 2015 Sep 03 [PMID: 26335930] (IHC-Fr, ICC/IF, Mouse)
• Tao L, Bei Y, Lin S et al. Exercise Training Protects Against Acute Myocardial Infarction via Improving Myocardial Energy Metabolism and Mitochondrial Biogenesis. Cell Physiol Biochem 2015 [PMID: 26303678]
• Biel TG, Lee S, Flores-Toro JA et al. Sirtuin 1 suppresses mitochondrial dysfunction of ischemic mouse livers in a mitofusin 2-dependent manner Cell Death Differ Feb 1 2016 [PMID: 26184910] (WB, Human)
• Kim J S, Kim B et al. Shear stress-induced mitochondrial biogenesis decreases the release of microparticles from endothelial cells. Am J Physiol Heart Circ Physiol Jan 8 2015 [PMID: 26024684] (WB, Human)
• Hu J, Lang Y, Cao Y et al. The Neuroprotective Effect of Tetramethylpyrazine Against Contusive Spinal Cord Injury by Activating PGC-1alpha in Rats. Neurochem. Res. 2015 May 16 [PMID: 25981953] (WB, IHC-Fr, Rat)
• Wang H, Bei Y, Lu Y et al. Exercise Prevents Cardiac Injury and Improves Mitochondrial Biogenesis in Advanced Diabetic Cardiomyopathy with PGC-1alpha and Akt Activation. Cell Physiol Biochem 2015 [PMID: 25896313] (Mouse)
• Kim B, Lee H, Kawata K, Park Jy. Exercise-mediated wall shear stress increases mitochondrial biogenesis in vascular endothelium PLoS OnE et al. 2014 Nov 07 [PMID: 25375175] (WB, Human)
  
  Details:
  PGC1 alpha antibody used for WB on lysates of human aortic ECs (HAECs) exposed to exercise-mimicking LSS at 20 dyne/cm2 for 48 hrs, and then, recovery (Rec) LSS at 5 dyne/cm2 was followed for another 24 hrs (Figure 1b).
• Yoshizawa Sayuri, Brown Andrew, Barchowsky Aaron, Sfeir Charles. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014 Aug 01 [PMID: 25158202]
• LaRocca TJ, Hearon CM, Henson GD, Seals DR. Mitochondrial quality control and age-associated arterial stiffening. Exp. Gerontol. 2014 Jul 14 [PMID: 25034910] (WB, Mouse)
  
  Details:
  PGC-1 alpha / PPAR gamma co-activator-1 alpha antibody used for WB (at 1:1000 dilution) in excised and cleaned thoracic aortas of male C57BL6 mice (young vs old) treated or not with trehalose. PGC-1 alpha studied as mitochondrial biogenesis/maintenance protein and WB image shown in Fig 1B.
• Roland CL, Arumugam T, Deng D et al. Cell surface lactate receptor GPR81 is crucial for cancer cell survival. Cancer Res. 2014 Jun 13 [PMID: 24928781] (IHC, Human)
  
  Details:
  IHC performed on primary human pancreatic cancer with 1:50 dilution of rabbit anti-PGC1 alpha
• Li M, Lu Y, Hu Y et al. Salvianolic acid B protects against acute ethanol-induced liver injury through SIRT1-mediated deacetylation of p53 in rats. Toxicol. Lett. 2014 Apr 21 [PMID: 24769256] (IP, Rat, Human)
• Yoshizawa S, Brown A, Barchowsky A. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation. Acta Biomater 2014 Feb 24 [PMID: 24512978] (WB, Human)
• Guo X, Dason ES, Zanon-Moreno V et al. PGC-1 alpha Signaling Coordinates Susceptibility to Metabolic and Oxidative Injury in the Inner Retina. Am. J. Pathol. 2014 Feb 28 [PMID: 24508229] (WB, ICC/IF, Mouse)
• Cho YR, Lim JH, Kim MY et al. Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice. PLoS One 2014 Jan 2 [PMID: 24392081] (WB, Human)
• Khan RS, Dine K, Das Sarma J, Shindler KS. SIRT1 Activating compounds reduce oxidative stress mediated neuronal loss in viral induced CNS demyelinating disease. Acta Neuropathol Commun 2014 Jan 2 [PMID: 24383546] (WB, Mouse)
• Nahirnyj A, Livne-Bar I, Guo X, Sivak JM. ROS Detoxification and Proinflammatory Cytokines Are Linked by p38 MAPK Signaling in a Model of Mature Astrocyte Activation. PLoS One 2013 Dec 23 [PMID: 24376630] (WB, Rat)
• Benderro GF, Tsipis CP, Sun X et al. Increased HIF-1alpha and HIF-2alpha Accumulation, but Decreased Microvascular Density, in Chronic Hyperoxia and Hypercapnia in the Mouse Cerebral Cortex. Adv Exp Med Biol 2013 [PMID: 23852473] (WB, Mouse)
• Fedorova LV, Sodhi K, Gatto-Weis C et al. Peroxisome Proliferator-Activated Receptor delta Agonist, HPP593, Prevents Renal Necrosis under Chronic Ischemia. PLoS One 2013 May 15 [PMID: 23691217] (WB, Rat)
• Xu R, Andres-Mateos E, Mejias R et al. Hibernating squirrel muscle activates the endurance exercise pathway despite prolonged immobilization. Exp Neurol 2013 Jan 16 [PMID: 23333568] (WB, Squirrel)
• Lim JH, Kim EN, Kim MY et al. Age-associated molecular changes in the kidney in aged mice Oxid Med Cell Longev 2012 [PMID: 23326623] (WB, Mouse)
• Khan RS, Fonseca-Kelly Z, Callinan C et al. SIRT1 activating compounds reduce oxidative stress and prevent cell death in neuronal cells Front Cell Neurosci 2012 [PMID: 23293585] (WB, Rat)
• Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle. 2012 Nov [PMID: 23070475] (WB, Human)
• Qiao C, Ma J, Xu J et al. Drosha mediates destabilization of Lin28 mRNA targets Cell Cycle 2012 Aug 30 [PMID: 22935707] (WB, Human)
• Benderro GF, Sun X, Kuang Y, Lamanna JC. Decreased VEGF expression and microvascular density, but increased HIF-1 and 2alpha accumulation and EPO expression in chronic moderate hyperoxia in the mouse brain Brain Res 2012 Jul 20 [PMID: 22820296] (WB, Mouse)
• Sun K, Asterholm IW, Kusminski CM et al. Dichotomous effects of VEGF-A on adipose tissue dysfunction Proc Natl Acad Sci U S A 2012 Mar 26 [PMID: 22451920] (WB, Mouse)
• Liu W, Singh R, Choi CS et al. LDL Receptor Related Protein 6 (LRP6) regulates body fat and glucose homeostasis by modulating nutrient sensing pathways and mitochondrial energy expenditure. The Journal of Biological Chemistry. 2012 Jan. [PMID: 22232553]
• Wu Y, Zhao J, Zhao W et al. Nandrolone normalizes determinants of muscle mass and fiber type after spinal cord injury. J Neurotrauma. 2012 May 20 [PMID: 22208735] (Rat)
• Rebholz SL, Jones T, Burke KT, Jaeschke A, Tso P, D'Alessio DA, Woollett LA. Multiparity leads to obesity and inflammation in mothers and obesity in male offspring. Am J Physiol Endocrinol Metab;302(4):E449-57. 2012 Feb. [PMID: 22127227]
• Benderro GF, Lamanna JC. Hypoxia-induced angiogenesis is delayed in aging mouse brain. Brain Res. 2011 Mar 11. [PMID: 21402058]
• Poomthavorn P, Wong SHX, Higgins S et al. Activation of a prometastatic gene expression program in hypoxic neuroblastoma cells. Endocr Relat Cancer;16(3):991-1004. 2009 [PMID: 19423615]

Reviews for PGC1 alpha Antibody (NBP1-04676) (11)

Average Rating: 4.6

(Based on 11 reviews)

We have 11 reviews tested in 6 species: Human, Mouse, Rat, Mouse and Human, Other, mus musculus and Mouse.

reviewed by: Robin Ren WB Mouse and Human 10/30/2018

Summary

• Application: Western Blot
• Sample Tested: Cell lysate, Sample Amount: 50ug-100ug protein
• Species: Mouse and Human
• Lot: G-8

reviewed by: Anonymous WB Human 12/14/2017

Summary

• Application: Western Blot
• Sample Tested: 3 human cancer cell lines
• Species: Human
• Lot: F-1

Comments

• Comments: AB DILUTIION: 1/1000

reviewed by: Anonymous WB Mouse 12/08/2017

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: G-1

Comments

• Comments: mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

reviewed by: Francesca Pacifici WB Mouse 09/26/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle homogenate
• Species: Mouse
• Lot: F-2

Comments

• Comments: Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.

reviewed by: Anonymous ChIP Rat 04/20/2017

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Adult spinal cord
• Species: Rat
• Lot: E-1

reviewed by: Harini Sampath WB mus musculus and Mouse 04/18/2017

Summary

• Application: Western Blot
• Sample Tested: mouse liver lysate
• Species: mus musculus and Mouse

reviewed by: Green Waggener WB Mouse 03/22/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: G1

Comments

• Comments: C57BL/6 Mice

reviewed by: Anonymous WB Rat 11/05/2015

Summary

• Application: Western Blot
• Sample Tested: Rat Liver Nuclear Protein Extract
• Species: Rat
• Lot: D-1

Blocking

• Blocking Details: 1x iBind Solution (Invitrogen) in iBind Western Device at room temperature

Primary Anitbody

• Dilution Ratio: used at 0.5ug/mL (dilution = 1:2000 in 1x iBind Solution) in iBind Western Device at room temperature

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: NEF812001EA
• Secondary Concentration: 1:5000

Details

• Detection Notes: PerkinElmer Western Lightning® Plus-ECL Enhanced Chemiluminescence Substrate, 1-second exposure

Comments

• Comments: We have used other PGC-1 alpha antibodies, but this one worked best!

reviewed by: Anonymous WB Rat 09/16/2015

Summary

• Application: Western Blot
• Sample Tested: rat spinal cord
• Species: Rat
• Lot: D-1

Blocking

• Blocking Details: 5% milk-PBST, 1hour, room temperature

Primary Anitbody

• Dilution Ratio: 1:2000, overnight, 4d.c, 2% BSA-PBS

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP
• Secondary Manufacturer Cat#: sc-2004
• Secondary Concentration: 1:4000

Details

• Detection Notes: ECL, BioRad, 50sec.

reviewed by: Anonymous WB Other 04/08/2015

Summary

• Application: Western Blot
• Species: Other
• Special Applications: I recently purchased a PGC1alpha antibody (NBP1-04676ss) for my study and was told if it worked in my animal model (Siberian Hamster) that I am eligible for a 100% credit voucher. Attached is the western blot images of white adipose tissue that was acquired using Novus' PGC1alpha antibody.
• File: View PDF

reviewed by: Giulia Ambrosi WB Human 04/23/2013

Summary

• Application: Western Blot
• Sample Tested: rat liver homogenates and human fibroblasts lysate
• Species: Human
• Lot: A-3
• Comments: Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Blocking

• Blocking Details: Left membrane: Odyssey (Li-Cor) blocking buffer. Right membrane: 5% Milk in PBS. Both 1hour at room temperature

Primary Anitbody

• Dilution Ratio: dilution (1:2000) in blocking buffer (either Odyssey block buffer or PBS+5%Milk) +0.1% Tween-20, overnight incubation at 4°C

Secondary Antibody

• Secondary Description: Donkey anti-rabbit IRDye 680, Odyssey Li-Cor
• Secondary Manufacturer Cat#: 926-32223
• Secondary Concentration: 1:10000

Details

• Detection Notes: Image analysis with a scanner from Odyssey near infrared technology. Scanner intensity: 5

Comments

• Comments: Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
2. What is the second band in the image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
   • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50 kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary
3. Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
   • According to UniProt, there are no methylated a.a. reported for this protein target.  However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen.  Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
4. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product? 
   • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.

Bioinformatics

• Gene Symbol: PPARGC1A
• Entrez:
  • 10891(Human)
  • 19017(Mouse)
  • 83516(Rat)
• Uniprot:
  • Q9UBK2(Human)