PGC1 alpha Antibody

Images

 
Genetic Strategies: Knockout Validated: PGC1 alpha Antibody [NBP1-04676] - Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane ...read more
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following ...read more
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong ...read more
Flow Cytometry: PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Gt, Ha, Sq, Ca, Eq, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

PGC1 alpha Antibody Summary

Immunogen
This PGC1 alpha Antibody was developed against a synthetic peptide made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
Localization
Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
Predicted Species
Canine (93%), Equine (94%), Primate (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARGC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/ml
  • Chromatin Immunoprecipitation
  • Flow Cytometry 1 - 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Flow (Intracellular) 1 - 2.5 ug/ml
  • Knockout Validated
Application Notes
In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID: 24508229). Use in IP reported in scientific literature (PMID: 24769256). Use in IHC-Frozen reported in scientific literature (PMID: 25981953). ChIP data based on customer review.
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PGC1 alpha Overexpression Lysate
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 11 Reviews rated 4.6
using
NBP1-04676 in the following applications:

Publications
Read Publications using
NBP1-04676 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32857105). Goat reactivity reported in scientific literature (PMID: 31158446)..

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody

  • LEM6
  • Ligand effect modulator 6
  • ligand effect modulator-6
  • L-PGC-1alpha
  • peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
  • peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
  • PGC1 alpha
  • PGC1
  • PGC-1(alpha)
  • PGC1A
  • PGC-1-alpha
  • PGC1APGC-1(alpha)
  • PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
  • PGC-1v
  • PPAR gamma coactivator variant form
  • PPAR gamma coactivator-1
  • PPARgamma coactivator 1alpha
  • PPAR-gamma coactivator 1-alpha
  • PPARGC1
  • PPARGC1A
  • PPARGC-1-alpha

Background

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O.,... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-04676
Species: Hu, Mu, Rt, Po, Gt, Ha, Sq, Ca, Eq, Pm
Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO

Publications for PGC1 alpha Antibody (NBP1-04676)(132)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Goat, Hamster, Porcine, Sheep, Squirrel, Zebrafish.

We have publications tested in 8 applications: ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
Flow
(2)
ICC/IF
(7)
IHC
(3)
IHC-Fr
(3)
IHC-P
(1)
IP
(1)
WB
(95)
All Applications
Filter By Species
Human
(30)
Mouse
(59)
Rat
(18)
Goat
(1)
Hamster
(1)
Porcine
(1)
Sheep
(1)
Squirrel
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 132. Show All 132 Publications.
Publications using NBP1-04676 Applications Species
Wen J, Li S, Zheng C et al. Tetramethylpyrazine nitrone improves motor dysfunction and pathological manifestations by activating the PGC-1 alpha/Nrf2/HO-1 pathway in ALS mice Neuropharmacology Nov 1 2020 [PMID: 33152451] (WB, Mouse) WB Mouse
Mu MD, Qian ZM, Yang SX et al. Sigma-1 receptor regulates mitophagy in dopaminergic neurons and contributes to dopaminergic protection Cell Death Dis Oct 26 2020 [PMID: 33116116]
Mukherjee D, Chander V, Bandyopadhyay A PARIS-DJ-1 Interaction Regulates Mitochondrial Functions in Cardiomyocytes which is Critically Important in Cardiac Hypertrophy Mol Cell Biol Oct 19 2020 [PMID: 33077496]
Subramani J, Kundumani-Sridharan V, Das KC Thioredoxin protects mitochondrial structure, function and biogenesis in myocardial ischemia-reperfusion via redox-dependent activation of AKT-CREB- PGC1 alpha pathway in aged mice Aging (Albany NY) Oct 13 2020 [PMID: 33049718] (WB, Human) WB Human
Rosa-Caldwell ME, Lim S, Haynie WS et al. Altering aspects of mitochondrial quality to improve musculoskeletal outcomes in disuse atrophy J. Appl. Physiol. Sep 17 2020 [PMID: 32940556]
Meyers K, Lopez M, Ho J et al. Lipocalin-2 deficiency may predispose to the progression of spontaneous age-related adiposity in mice Sci Rep Sep 3 2020 [PMID: 32883997]
Wang D, Yang Y, Zou X et al. Antioxidant Apigenin Relieves Age-Related Muscle Atrophy by Inhibiting Oxidative Stress and Hyperactive Mitophagy and Apoptosis in Skeletal Muscle of Mice J. Gerontol. A Biol. Sci. Med. Sci. Aug 28 2020 [PMID: 32857105] (WB, Mouse) WB Mouse
TEglAs T, AbrahAm D, JOkai M et al. Exercise combined with a probiotics treatment alters the microbiome, but moderately affects signalling pathways in the liver of male APP/PS1 transgenic mice Biogerontology Aug 18 2020 [PMID: 32812166] (WB, Mouse) WB Mouse
Kumar M, Acevedo-CintrOn J, Jhaldiyal A et al. Defects in Mitochondrial Biogenesis Drive Mitochondrial Alterations in PARKIN-Deficient Human Dopamine Neurons Stem Cell Reports Jul 30 2020 [PMID: 32795422] (WB, IHC, Human) WB, IHC Human
Aljaylani A, Fluitt M, Piselli A et al. Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice Cell. Physiol. Biochem. Jul 18 2020 [PMID: 32678535]
Show All 132 Publications.

Reviews for PGC1 alpha Antibody (NBP1-04676) (11) 4.611

Average Rating: 4.6
(Based on 11 reviews)
We have 11 reviews tested in 6 species: Human, Mouse, Rat, Mouse and Human, Other, mus musculus and Mouse.

Reviews using NBP1-04676:
Filter by Applications
WB
(10)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rat
(3)
Mouse and Human
(1)
Other
(1)
mus musculus and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Robin Ren
WB Mouse and Human 10/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate, Sample Amount: 50ug-100ug protein
SpeciesMouse and Human
LotG-8
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Anonymous
WB Human 12/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
LotF-1

Comments

CommentsAB DILUTIION: 1/1000
Western Blot PGC1 alpha NBP1-04676
Enlarge
4
reviewed by:
Anonymous
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotG-1

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Francesca Pacifici
WB Mouse 09/26/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotF-2

Comments

CommentsWestern Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.
  5
reviewed by:
Anonymous
ChIP Rat 04/20/2017
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedAdult spinal cord
SpeciesRat
LotE-1
  4
reviewed by:
Harini Sampath
WB mus musculus and Mouse 04/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
Speciesmus musculus and Mouse
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Green Waggener
WB Mouse 03/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotG1

Comments

CommentsC57BL/6 Mice
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Anonymous
WB Rat 11/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat Liver Nuclear Protein Extract
SpeciesRat
LotD-1

Blocking

Blocking Details1x iBind Solution (Invitrogen) in iBind Western Device at room temperature

Primary Anitbody

Dilution Ratioused at 0.5ug/mL (dilution = 1:2000 in 1x iBind Solution) in iBind Western Device at room temperature

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#NEF812001EA
Secondary Concentration1:5000

Details

Detection NotesPerkinElmer Western Lightning® Plus-ECL Enhanced Chemiluminescence Substrate, 1-second exposure

Comments

CommentsWe have used other PGC-1 alpha antibodies, but this one worked best!
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Anonymous
WB Rat 09/16/2015
View

Summary

ApplicationWestern Blot
Sample Testedrat spinal cord
SpeciesRat
LotD-1

Blocking

Blocking Details5% milk-PBST, 1hour, room temperature

Primary Anitbody

Dilution Ratio1:2000, overnight, 4d.c, 2% BSA-PBS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:4000

Details

Detection NotesECL, BioRad, 50sec.
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Anonymous
WB Other 04/08/2015
View

Summary

ApplicationWestern Blot
SpeciesOther
Special ApplicationsI recently purchased a PGC1alpha antibody (NBP1-04676ss) for my study and was told if it worked in my animal model (Siberian Hamster) that I am eligible for a 100% credit voucher. Attached is the western blot images of white adipose tissue that was acquired using Novus' PGC1alpha antibody.
FileView PDF
Western Blot PGC1 alpha NBP1-04676
Enlarge
3
reviewed by:
Giulia Ambrosi
WB Human 04/23/2013
View

Summary

ApplicationWestern Blot
Sample Testedrat liver homogenates and human fibroblasts lysate
SpeciesHuman
LotA-3
CommentsConsidering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Blocking

Blocking DetailsLeft membrane: Odyssey (Li-Cor) blocking buffer. Right membrane: 5% Milk in PBS. Both 1hour at room temperature

Primary Anitbody

Dilution Ratiodilution (1:2000) in blocking buffer (either Odyssey block buffer or PBS+5%Milk) +0.1% Tween-20, overnight incubation at 4°C

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IRDye 680, Odyssey Li-Cor
Secondary Manufacturer Cat#926-32223
Secondary Concentration1:10000

Details

Detection NotesImage analysis with a scanner from Odyssey near infrared technology. Scanner intensity: 5

Comments

CommentsConsidering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. What is the second band in the image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
    • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50 kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary
  3. Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
    • According to UniProt, there are no methylated a.a. reported for this protein target.  However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen.  Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
  4. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product? 
    • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-04676AF350
Alexa Fluor 405 NBP1-04676AF405
Alexa Fluor 488 NBP1-04676AF488
Alexa Fluor 532 NBP1-04676AF532
Alexa Fluor 594 NBP1-04676AF594
Alexa Fluor 647 NBP1-04676AF647
Alexa Fluor 700 NBP1-04676AF700
Alexa Fluor 750 NBP1-04676AF750
Allophycocyanin NBP1-04676APC
Biotin NBP1-04676B
DyLight 350 NBP1-04676UV
DyLight 405 NBP1-04676V
DyLight 488 NBP1-04676G
DyLight 550 NBP1-04676R
DyLight 594 NBP1-04676DL594
DyLight 650 NBP1-04676C
DyLight 680 NBP1-04676FR
DyLight 755 NBP1-04676IR
FITC NBP1-04676F
HRP NBP1-04676H
Janelia Fluor 549 NBP1-04676JF549
Janelia Fluor 646 NBP1-04676JF646
PE NBP1-04676PE
PerCP NBP1-04676PCP

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Recent Reviews

4.6
5
8
4
2
3
1
2
0
1
0

Robin Ren
10/30/2018
Application: WB
Species: Mouse and Human

Anonymous
12/14/2017
Application: WB
Species: Human

Anonymous
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARGC1A
Entrez
Uniprot