PGC1 alpha Antibody

Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-PGC1-alpha in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of PGC-1 alpha in mouse prostate using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - IHC analysis of a formalin-fixed paraffin-embedded tissue section of mouse prostate using rabbit polyclonal PGC1 alpha antibody at 1:200 dilution. The antibody generated an expected strong nuclear (punctate in some cells) and a weak cytoplasmic staining in the glandular cells lining of tubule-alveolar gland.

Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue. This image was submitted via customer Review.

Immunocytochemistry/Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGC-1 alpha [NBP1-04676] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with PGC1 alpha Antibody NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody. Image from verified customer review.

• Reactivity: Hu, Mu, Rt, Ha, Sq, Ca, Eq, Pm
• Applications: WB, ChIP, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

PGC1 alpha Antibody Summary

• Immunogen: Synthetic peptide made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
• Localization: Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
• Predicted Species: Equine (94%), Primate (98%), Canine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPARGC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Flow (Intracellular)
• Application Notes: In WB, a band is seen at ~91 kDa representing PGC-1 alpha. In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID 24508229). Use in IP reported in scientific literature (PMID 24769256). Use in IHC-Frozen reported in scientific literature (PMID 25981953). ChIP data based on customer review.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Positive Control:

  PGC1 alpha Lysate (NBL1-14636)
  Skeletal Muscle Lysate (NB820-59668)

Reactivity Notes

Expected reactivity based on sequence identity: monkey/non-human primates (98%), equine (94%), and canine (93%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody

• LEM6
• Ligand effect modulator 6
• ligand effect modulator-6
• peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
• peroxisome proliferator-activated receptor gamma coactivator 1-alpha
• peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• PGC1 alpha
• PGC1A
• PGC-1-alpha
• PGC1APGC-1(alpha)
• PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
• PPAR gamma coactivator variant form
• PPAR gamma coactivator-1
• PPAR-gamma coactivator 1-alpha
• PPARGC1A
• PPARGC-1-alpha
• PPARGC1PGC-1v

Background

Named after its association with nuclear receptor peroxisome-proliferator activated receptor (PPAR) gamma, PGC-1 alpha (PPAR gamma coactivator 1-alpha) is now known to interact with a variety of transcription factors to regulate multiple aspects of cellular physiology. PGC-1 alpha binds MYBBP1A which results in inhibition of PGC-1 alpha's transcriptional activation potential and also interact with other proteins such as PRDM16, LRPPRC, LPIN1 etc. IRSs with core sequence T(G/A)TTTT serves as PGC-1 alpha's promoter and its transcription is repressed by ZNF746. CLK2 and AMPK execute PGC-1 alpha's phosphorylation and in the skeletal muscle its phosphorylation by AMPK increases activation of its own promoter. Under the condition of high nutrients, PGC-1 alpha is heavily acetylated by GCN5 resulting in its biological inactivation whereas it gets deacetylated by SIRT1 in low nutrients/high NAD conditions. PGC-1alpha acts as transcriptional coactivator for steroid and nuclear receptors, and enhances the transcriptional activity of PPARG as well as the thyroid hormone receptor on the uncoupling protein promoter. PGC-1alpha regulates key mitochondrial genes that contribute towards adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGC1 alpha Antibody (NBP1-04676)(46)

Publications using NBP1-04676

• Unno Y, Yamamoto H, Takatsuki S et al. Palmitoyl lactic acid induces adipogenesis and a brown fat-like phenotype in 3T3-L1 preadipocytes. Biochim. Biophys. Acta Apr 12 2018 [PMID: 29654827] (Mouse)
• Wang D, Sun H, Song G et al. Resveratrol Improves Muscle Atrophy By Modulating Mitochondrial Quality Control In STZ-Induced Diabetic Mice. Mol Nutr Food Res. Mar 26 2018 [PMID: 29578301] (WB, Rat)
• Munoz VR, Gaspar RC, Kuga GK et al. Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation. J. Cell. Biochem. Mar 25 2018 [PMID: 29575149] (Mouse)
• Huang C, Wang K, Yang Y et al. Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation Phytomedicine 2018 Jan 01 [PMID: 29433686] (Rat)
• Zhang W, Zhong W, Sun Q et al. Adipose-specific lipin1 overexpression in mice protects against alcohol-induced liver injury Sci Rep 2018 Jan 11 [PMID: 29323242] (WB, Mouse)
• Bauters D, Bedossa P, Lijnen HR, Hemmeryckx B. Functional role of ADAMTS5 in adiposity and metabolic health PLoS ONE 2018 Jan 02 [PMID: 29293679] (WB, Mouse)
• Geoghegan F, Buckland RJ, Rogers E et al. Bioenergetics of acquired cisplatin resistant H1299 non-small cell lung cancer and P31 mesothelioma cells Oncotarget. 2017 Oct 16 [PMID: 29212260] (WB, Human)
• Fakouri NB, Durhuus JA, Regnell CE et al. Rev1 contributes to proper mitochondrial function via the PARP-NAD(+)-SIRT1-PGC1a axis. Sci Rep. 2017 Oct 02 [PMID: 28970491] (WB, Mouse)
• Wang D, Chen J, Liu X et al. A Chinese herbal formula, Jian-Pi-Yi-Shen decoction, improves muscle atrophy via regulating mitochondrial quality control process in 5/6 nephrectomised rats Sci Rep 2017 Aug 23 [PMID: 28835671] (WB, Rat)
• Chung E, Joiner HE, Skelton T et al. Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts. Physiol Rep. Mar 1 2017 [PMID: 28292876] (WB, Mouse)
• Kemter E, Frohlich T, Arnold GJ et al. Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease -UMOD (ADTKD-UMOD) Sci Rep. [PMID: 28220896] (WB, Mouse)
• Wang G, Song Y, Feng W et al. Activation of AMPK attenuates LPS-induced acute lung injury by upregulation of PGC1alpha and SOD1. Exp Ther Med. Sep 1 2016 [PMID: 27602077] (WB, Mouse)
• Oliviero M, Romilde I, Beatrice MM et al. Evaluations of thyme extract effects in human normal bronchial and tracheal epithelial cell lines and in human lung cancer cell line Chem. Biol. Interact. 2016 Jun 29 [PMID: 27369807] (WB, Human)
• Liu X, Wu J, Wang J et al. Possible role of mitochondrial injury in Caulis Aristolochia manshuriensis-induced chronic aristolochic acid nephropathy. Drug Chem Toxicol Jun 2 2016 [PMID: 27250112]
• Ivankovic D, Chau KY, Schapira AH, Gegg ME. Mitochondrial and lysosomal biogenesis are activated following PINK1/parkin-mediated mitophagy. J. Neurochem. 2015 Oct 28 [PMID: 26509433] (WB, Human)
  
  Details:
  PGC1 alpha antibody was used for WB analysis of total cell lysates of SH-SY5Y cells, TFEB-DDK cells and SH-SY5Y cells over-expressing human PGC-1a gene. WB was also performed on nuclear and cytosolic fractions of TFEB cells which were treated with 10 uM of carbonyl cyanide m- chlorophenylhydrazone(CCCP).
• De Andrade PB, Neff LA, Strosova MK et al. Caloric restriction induces energy-sparing alterations in skeletal muscle contraction, fiber composition and local thyroid hormone metabolism that persist during catch-up fat upon refeeding. Front Physiol 2015 [PMID: 26441673]
• Kishida T, Ejima A, Yamamoto K et al. Reprogrammed Functional Brown Adipocytes Ameliorate Insulin Resistance and Dyslipidemia in Diet-Induced Obesity and Type 2 Diabetes. Stem Cell Reports 2015 Sep 09 [PMID: 26365511] (ICC/IF, Human)
• Liu B, Zhang R, Tao G et al. Augmented Wnt-Signaling as a Therapeutic Tool to Prevent Ischemia and Reperfusion Injury in Liver: Preclinical Studies in a Mouse Model. Liver Transpl. 2015 Sep 03 [PMID: 26335930] (IHC-Fr, ICC/IF, Mouse)
• Tao L, Bei Y, Lin S et al. Exercise Training Protects Against Acute Myocardial Infarction via Improving Myocardial Energy Metabolism and Mitochondrial Biogenesis. Cell Physiol Biochem 2015 [PMID: 26303678]
• Biel TG, Lee S, Flores-Toro JA et al. Sirtuin 1 suppresses mitochondrial dysfunction of ischemic mouse livers in a mitofusin 2-dependent manner Cell Death Differ Feb 1 2016 [PMID: 26184910] (WB, Human)
• Hu J, Lang Y, Cao Y et al. The Neuroprotective Effect of Tetramethylpyrazine Against Contusive Spinal Cord Injury by Activating PGC-1alpha in Rats. Neurochem. Res. 2015 May 16 [PMID: 25981953] (WB, IHC-Fr, Rat)
• Wang H, Bei Y, Lu Y et al. Exercise Prevents Cardiac Injury and Improves Mitochondrial Biogenesis in Advanced Diabetic Cardiomyopathy with PGC-1alpha and Akt Activation. Cell Physiol Biochem 2015 [PMID: 25896313] (Mouse)
• Kim B, Lee H, Kawata K, Park Jy. Exercise-mediated wall shear stress increases mitochondrial biogenesis in vascular endothelium PLoS OnE et al. 2014 Nov 07 [PMID: 25375175] (WB, Human)
  
  Details:
  PGC1 alpha antibody used for WB on lysates of human aortic ECs (HAECs) exposed to exercise-mimicking LSS at 20 dyne/cm2 for 48 hrs, and then, recovery (Rec) LSS at 5 dyne/cm2 was followed for another 24 hrs (Figure 1b).
• Yoshizawa Sayuri, Brown Andrew, Barchowsky Aaron, Sfeir Charles. Role of magnesium ions on osteogenic response in bone marrow stromal cells. Connect Tissue Res. 2014 Aug 01 [PMID: 25158202]
• LaRocca TJ, Hearon CM, Henson GD, Seals DR. Mitochondrial quality control and age-associated arterial stiffening. Exp. Gerontol. 2014 Jul 14 [PMID: 25034910] (WB, Mouse)
  
  Details:
  PGC-1 alpha / PPAR gamma co-activator-1 alpha antibody used for WB (at 1:1000 dilution) in excised and cleaned thoracic aortas of male C57BL6 mice (young vs old) treated or not with trehalose. PGC-1 alpha studied as mitochondrial biogenesis/maintenance protein and WB image shown in Fig 1B.
• Roland CL, Arumugam T, Deng D et al. Cell surface lactate receptor GPR81 is crucial for cancer cell survival. Cancer Res. 2014 Jun 13 [PMID: 24928781] (IHC, Human)
  
  Details:
  IHC performed on primary human pancreatic cancer with 1:50 dilution of rabbit anti-PGC1 alpha
• Li M, Lu Y, Hu Y et al. Salvianolic acid B protects against acute ethanol-induced liver injury through SIRT1-mediated deacetylation of p53 in rats. Toxicol. Lett. 2014 Apr 21 [PMID: 24769256] (IP, Rat, Human)
• Yoshizawa S, Brown A, Barchowsky A. Magnesium ion stimulation of bone marrow stromal cells enhances osteogenic activity, simulating the effect of magnesium alloy degradation. Acta Biomater 2014 Feb 24 [PMID: 24512978] (WB, Human)
• Guo X, Dason ES, Zanon-Moreno V et al. PGC-1 alpha Signaling Coordinates Susceptibility to Metabolic and Oxidative Injury in the Inner Retina. Am. J. Pathol. 2014 Feb 28 [PMID: 24508229] (WB, ICC/IF, Mouse)
• Cho YR, Lim JH, Kim MY et al. Therapeutic Effects of Fenofibrate on Diabetic Peripheral Neuropathy by Improving Endothelial and Neural Survival in db/db Mice. PLoS One 2014 Jan 2 [PMID: 24392081] (WB, Human)
• Khan RS, Dine K, Das Sarma J, Shindler KS. SIRT1 Activating compounds reduce oxidative stress mediated neuronal loss in viral induced CNS demyelinating disease. Acta Neuropathol Commun 2014 Jan 2 [PMID: 24383546] (WB, Mouse)
• Nahirnyj A, Livne-Bar I, Guo X, Sivak JM. ROS Detoxification and Proinflammatory Cytokines Are Linked by p38 MAPK Signaling in a Model of Mature Astrocyte Activation. PLoS One 2013 Dec 23 [PMID: 24376630] (WB, Rat)
• Benderro GF, Tsipis CP, Sun X et al. Increased HIF-1alpha and HIF-2alpha Accumulation, but Decreased Microvascular Density, in Chronic Hyperoxia and Hypercapnia in the Mouse Cerebral Cortex. Adv Exp Med Biol 2013 [PMID: 23852473] (WB, Mouse)
• Fedorova LV, Sodhi K, Gatto-Weis C et al. Peroxisome Proliferator-Activated Receptor delta Agonist, HPP593, Prevents Renal Necrosis under Chronic Ischemia. PLoS One 2013 May 15 [PMID: 23691217] (WB, Rat)
• Xu R, Andres-Mateos E, Mejias R et al. Hibernating squirrel muscle activates the endurance exercise pathway despite prolonged immobilization. Exp Neurol 2013 Jan 16 [PMID: 23333568] (WB, Squirrel)
• Lim JH, Kim EN, Kim MY et al. Age-associated molecular changes in the kidney in aged mice Oxid Med Cell Longev 2012 [PMID: 23326623] (WB, Mouse)
• Khan RS, Fonseca-Kelly Z, Callinan C et al. SIRT1 activating compounds reduce oxidative stress and prevent cell death in neuronal cells Front Cell Neurosci 2012 [PMID: 23293585] (WB, Rat)
• Salem AF, Whitaker-Menezes D, Howell A et al. Mitochondrial biogenesis in epithelial cancer cells promotes breast cancer tumor growth and confers autophagy resistance. Cell Cycle. 2012 Nov [PMID: 23070475] (WB, Human)
• Qiao C, Ma J, Xu J et al. Drosha mediates destabilization of Lin28 mRNA targets Cell Cycle 2012 Aug 30 [PMID: 22935707] (WB, Human)
• Benderro GF, Sun X, Kuang Y, Lamanna JC. Decreased VEGF expression and microvascular density, but increased HIF-1 and 2alpha accumulation and EPO expression in chronic moderate hyperoxia in the mouse brain Brain Res 2012 Jul 20 [PMID: 22820296] (WB, Mouse)
• Sun K, Asterholm IW, Kusminski CM et al. Dichotomous effects of VEGF-A on adipose tissue dysfunction Proc Natl Acad Sci U S A 2012 Mar 26 [PMID: 22451920] (WB, Mouse)
• Liu W, Singh R, Choi CS et al. LDL Receptor Related Protein 6 (LRP6) regulates body fat and glucose homeostasis by modulating nutrient sensing pathways and mitochondrial energy expenditure. The Journal of Biological Chemistry. 2012 Jan. [PMID: 22232553]
• Wu Y, Zhao J, Zhao W et al. Nandrolone normalizes determinants of muscle mass and fiber type after spinal cord injury. J Neurotrauma. 2012 May 20 [PMID: 22208735] (Rat)
• Rebholz SL, Jones T, Burke KT, Jaeschke A, Tso P, D'Alessio DA, Woollett LA. Multiparity leads to obesity and inflammation in mothers and obesity in male offspring. Am J Physiol Endocrinol Metab;302(4):E449-57. 2012 Feb. [PMID: 22127227]
• Benderro GF, Lamanna JC. Hypoxia-induced angiogenesis is delayed in aging mouse brain. Brain Res. 2011 Mar 11. [PMID: 21402058]
• Poomthavorn P, Wong SHX, Higgins S et al. Activation of a prometastatic gene expression program in hypoxic neuroblastoma cells. Endocr Relat Cancer;16(3):991-1004. 2009 [PMID: 19423615]

Reviews for PGC1 alpha Antibody (NBP1-04676) (10)

Average Rating: 4.6

(Based on 10 reviews)

We have 10 reviews tested in 5 species: Human, Mouse, Rat, Other, mus musculus and Mouse.

reviewed by: Anonymous WB Human 12/14/2017

Summary

• Application: Western Blot
• Sample Tested: 3 human cancer cell lines
• Species: Human
• Lot: F-1

Comments

• Comments: AB DILUTIION: 1/1000

reviewed by: Anonymous WB Mouse 12/08/2017

Summary

• Application: Western Blot
• Sample Tested: Primary mouse hepatocytes
• Species: Mouse
• Lot: G-1

Comments

• Comments: mouse hepatocytes were treated with 100 ng/ml LPS for 4 hours

reviewed by: Francesca Pacifici WB Mouse 09/26/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle homogenate
• Species: Mouse
• Lot: F-2

Comments

• Comments: Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.

reviewed by: Anonymous ChIP Rat 04/20/2017

Summary

• Application: Chromatin Immunoprecipitation
• Sample Tested: Adult spinal cord
• Species: Rat
• Lot: E-1

reviewed by: Harini Sampath WB mus musculus and Mouse 04/18/2017

Summary

• Application: Western Blot
• Sample Tested: mouse liver lysate
• Species: mus musculus and Mouse

reviewed by: Green Waggener WB Mouse 03/22/2017

Summary

• Application: Western Blot
• Sample Tested: Mouse skeletal muscle
• Species: Mouse
• Lot: G1

Comments

• Comments: C57BL/6 Mice

reviewed by: Anonymous WB Rat 11/05/2015

Summary

• Application: Western Blot
• Sample Tested: Rat Liver Nuclear Protein Extract
• Species: Rat
• Lot: D-1

Blocking

• Blocking Details: 1x iBind Solution (Invitrogen) in iBind Western Device at room temperature

Primary Anitbody

• Dilution Ratio: used at 0.5ug/mL (dilution = 1:2000 in 1x iBind Solution) in iBind Western Device at room temperature

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG, HRP
• Secondary Manufacturer Cat#: NEF812001EA
• Secondary Concentration: 1:5000

Details

• Detection Notes: PerkinElmer Western Lightning® Plus-ECL Enhanced Chemiluminescence Substrate, 1-second exposure

Comments

• Comments: We have used other PGC-1 alpha antibodies, but this one worked best!

reviewed by: Anonymous WB Rat 09/16/2015

Summary

• Application: Western Blot
• Sample Tested: rat spinal cord
• Species: Rat
• Lot: D-1

Blocking

• Blocking Details: 5% milk-PBST, 1hour, room temperature

Primary Anitbody

• Dilution Ratio: 1:2000, overnight, 4d.c, 2% BSA-PBS

Secondary Antibody

• Secondary Description: goat anti-rabbit IgG-HRP
• Secondary Manufacturer Cat#: sc-2004
• Secondary Concentration: 1:4000

Details

• Detection Notes: ECL, BioRad, 50sec.

reviewed by: Anonymous WB Other 04/08/2015

Summary

• Application: Western Blot
• Species: Other
• Special Applications: I recently purchased a PGC1alpha antibody (NBP1-04676ss) for my study and was told if it worked in my animal model (Siberian Hamster) that I am eligible for a 100% credit voucher. Attached is the western blot images of white adipose tissue that was acquired using Novus' PGC1alpha antibody.
• File: View PDF

reviewed by: Giulia Ambrosi WB Human 04/23/2013

Summary

• Application: Western Blot
• Sample Tested: rat liver homogenates and human fibroblasts lysate
• Species: Human
• Lot: A-3
• Comments: Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Blocking

• Blocking Details: Left membrane: Odyssey (Li-Cor) blocking buffer. Right membrane: 5% Milk in PBS. Both 1hour at room temperature

Primary Anitbody

• Dilution Ratio: dilution (1:2000) in blocking buffer (either Odyssey block buffer or PBS+5%Milk) +0.1% Tween-20, overnight incubation at 4°C

Secondary Antibody

• Secondary Description: Donkey anti-rabbit IRDye 680, Odyssey Li-Cor
• Secondary Manufacturer Cat#: 926-32223
• Secondary Concentration: 1:10000

Details

• Detection Notes: Image analysis with a scanner from Odyssey near infrared technology. Scanner intensity: 5

Comments

• Comments: Considering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

1. Please differentiate me between PPAR and PGC clearly.i am confuse to know the differ these two. waiting for your reply
   • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
2. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product?
   • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.
3. What is the percent homology between the human sequence used as the immunogen and the corresponding region in mouse?
   • The immunogen for NBP1-04676 shares over 92% homology with mouse and is guaranteed to cross-react. We have IHC data from internal testing on mouse tissues, as well as Western blot data from multiple publications.
4. What is the second band in the Image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
   • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary.

Bioinformatics

• Gene Symbol: PPARGC1A
• Entrez:
  • 10891(Human)
  • 19017(Mouse)
  • 83516(Rat)
• Uniprot:
  • Q9UBK2(Human)