PGC1 alpha Antibody - BSA Free

Images

 
Genetic Strategies: Knockout Validated: PGC1 alpha Antibody [NBP1-04676] - Western blot shows lysates of A431 human squamous carcinoma parental cell line and PGC1 alpha knockout (KO) A431 cell line. PVDF membrane ...read more
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from human adipose and skeletal muscle tissue, HeLa and A431 cells lines was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked ...read more
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of PGC1 alpha Antibody in mouse prostate using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HepG2 cells with PGC1 alpha Antibody and a matched isotype control. Cells were fixed with 4% PFA and then permeablized ...read more
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Western Blot of total protein from murine skeletal muscle tissue using PGC1 alpha Antibody. Image from verified customer review.
Western Blot: PGC1 alpha Antibody [NBP1-04676] - Mitochondrial content and biogenesis. Immunoblot analysis of PGC1 alpha in MEFs (n = 7; t = 5.764; df = 6). Image collected and cropped by CiteAb from the following ...read more
Immunocytochemistry/ Immunofluorescence: PGC1 alpha Antibody [NBP1-04676] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Immunohistochemistry-Paraffin: PGC1 alpha Antibody [NBP1-04676] - Analysis of a FFPE section of mouse prostate using rabbit polyclonal PGC1 alpha Antibody at 1:200 dilution. The antibody generated an expected strong ...read more
Flow Cytometry: PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on A431 cells with PGC1 alpha Antibody NBP1-04676 (blue) and matched isotype control NBP2-24891 (orange). Cells were fixed with 4% ...read more
Flow (Intracellular): PGC1 alpha Antibody [NBP1-04676] - An intracellular stain was performed on HeLa cells with NBP1-04676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, PmSpecies Glossary
Applications WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

PGC1 alpha Antibody - BSA Free Summary

Immunogen
This PGC1 alpha Antibody was developed against a recombinant protein made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
Localization
Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
Predicted Species
Canine (93%), Equine (94%), Primate (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARGC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Flow (Intracellular) 1 - 2.5 ug/ml
  • Flow Cytometry 1 - 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1 - 2 ug/ml
Application Notes
In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in ICC reported in scientific literature (PMID: 24508229). Use in IP reported in scientific literature (PMID: 24769256). Use in IHC-Frozen reported in scientific literature (PMID: 25981953). ChIP data based on customer review.
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 11 Reviews rated 4.6
using
NBP1-04676 in the following applications:

Publications
Read Publications using
NBP1-04676 in the following applications:

  • 1 publication
  • 3 publications
  • 9 publications
  • IHC
    5 publications
  • 4 publications
  • 2 publications
  • IP
    2 publications
  • WB
    131 publications

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34573421). Use in Mouse reported in scientific literature (PMID:33719499). Expected from sequence similarity: Mouse Use in Sheep reported in scientific literature (PMID:32403966).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGC1 alpha Antibody - BSA Free

  • LEM6
  • Ligand effect modulator 6
  • ligand effect modulator-6
  • L-PGC-1alpha
  • peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-3ext
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B4-8a
  • peroxisome proliferator-activated receptor gamma coactivator 1 alpha transcript variant B5-NT
  • peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
  • PGC1 alpha
  • PGC1
  • PGC-1(alpha)
  • PGC1A
  • PGC-1-alpha
  • PGC1APGC-1(alpha)
  • PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
  • PGC-1v
  • PPAR gamma coactivator variant form
  • PPAR gamma coactivator-1
  • PPARgamma coactivator 1alpha
  • PPAR-gamma coactivator 1-alpha
  • PPARGC1
  • PPARGC1A
  • PPARGC-1-alpha

Background

Peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1alpha), PPAR-gamma coactivator 1-alpha, PPARGC-1-alpha (theoretical molecular weight 91 kDa) belongs to a family of transcription coactivators which includes two other proteins; PGC-1beta and PGC-1-related coactivators. PGC1 alpha interacts with various transcription factors (e.g., FOXO1, PPAR-(alpha, delta and gamma), LXR and FXR), promoting the transactivation of their specific target genes (1). As a transcription coactivator, PGC1 alpha is found in the nucleus where it interacts with transcriptional activators and chromatin modifiers such as p300/CBP and SRC-1 to induce targeted gene expression (1, 2). Transcription factors which interact with PGC1 alpha are involved in several functions related to cellular energy homeostasis including mitochondrial biogenesis, glucose and fatty acid metabolism, and skeletal muscle remodeling. Upstream regulators of PGC1 alpha activity include proteins that play a central role as energy sensors including SIRT1 and AMPK which, through different mechanisms, induce PGC1 alpha activity (3).

PGC1 alpha is highly expressed in skeletal muscle, cardiac muscle, and brown adipose tissue, all tissues with high oxidative capacity (1, 2). PGC1 alpha is also induced by energy taxing physiological states, for example increased physical activity, reduced body temperature, and reduced food intake (3). Because of its role in pathways controlling cellular energy expenditure, PGC1 alpha dysfunction has been associated with pathophysiological conditions such as type 2 diabetes and the metabolic syndrome (3).

References

1.Liang, H., & Ward, W. F. (2006). PGC-1alpha: A key regulator of energy metabolism. American Journal of Physiology - Advances in Physiology Education. https://doi.org/10.1152/advan.00052.2006

2.Arany, Z., He, H., Lin, J., Hoyer, K., Handschin, C., Toka, O., ... Spiegelman, B. M. (2005). Transcriptional coactivator PGC-1alpha controls the energy state and contractile function of cardiac muscle. Cell Metabolism. https://doi.org/10.1016/j.cmet.2005.03.002

3.Canto, C., & Auwerx, J. (2009). PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Current Opinion in Lipidology. https://doi.org/10.1097/MOL.0b013e328328d0a4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-04676
Species: Hu, Mu, Rt, Po, Gt, Ha, Sh, Sq, Ca, Eq, Pm
Applications: WB, ChIP, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KO

Publications for PGC1 alpha Antibody (NBP1-04676)(184)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Chicken, Goat, Hamster, Porcine, Sheep, Squirrel, Zebrafish.

We have publications tested in 8 applications: ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB.


Filter By Application
ChIP
(1)
Flow
(3)
ICC/IF
(9)
IHC
(5)
IHC-Fr
(4)
IHC-P
(2)
IP
(2)
WB
(131)
All Applications
Filter By Species
Human
(38)
Mouse
(76)
Rat
(24)
Chicken
(1)
Goat
(1)
Hamster
(1)
Porcine
(1)
Sheep
(1)
Squirrel
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 184. Show All 184 Publications.
Publications using NBP1-04676 Applications Species
Hu L, Guo Y, Song L et al. Nicotinamide Riboside Promotes Mfn2-mediated Mitochondrial Fusion in Diabetic Hearts through the SIRT1-PGC1 alpha-PPAR alpha Pathway Research Square Dec 30 2021 (WB, Mouse) WB Mouse
Shin J, Hong S, Choi S et al. Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner Redox Biology Apr 1 2022 [PMID: 35121402] (WB, Mouse) WB Mouse
Kim KH, Lee KA Metabolic Rewiring by Human Placenta-Derived Mesenchymal Stem Cell Therapy Promotes Rejuvenation in Aged Female Rats International journal of molecular sciences Jan 5 2022 [PMID: 35008991] (WB) WB
Kawarasaki S, Matsuo K, Kuwata H et al. Screening of flavor compounds using Ucp1-luciferase reporter beige adipocytes identified 5-methylquinoxaline as a novel UCP1-inducing compoundsss Bioscience, biotechnology, and biochemistry Dec 22 2021 [PMID: 34935880]
Raghavan S, Kundumani-Sridharan V, Kumar S et al. Thioredoxin Prevents Loss of UCP2 in Hyperoxia via MKK4-p38 MAPK- PGC1 alpha Signaling and Limits Oxygen Toxicity American journal of respiratory cell and molecular biology Dec 10 2021 [PMID: 34890296]
Awasthi S, Hindle A, Sawant NA Et al. RALBP1 in Oxidative Stress and Mitochondrial Dysfunction in Alzheimer's Disease Cells Nov 10 2021 [PMID: 34831336]
Longo M, Meroni M, Paolini E Et al. TM6SF2/PNPLA3/MBOAT7 loss-of-function genetic variants impact on NAFLD development and progression both in patients and in in vitro models Cellular and molecular gastroenterology and hepatology Nov 22 2021 [PMID: 34823063] (WB, ICC/IF) WB, ICC/IF
Kshirsagar S, Sawant N, Morton H Et al. Mitophagy enhancers against phosphorylated Tau-induced mitochondrial and synaptic toxicities in Alzheimer disease Pharmacological research Nov 8 2021 [PMID: 34763094] (WB, Mouse) WB Mouse
Bhat N, Narayanan A, Fathzadeh M Et al. Dyrk1b promotes autophagy during skeletal muscle differentiation by upregulating 4e-bp1 Cellular signalling Nov 6 2021 [PMID: 34752933] (WB) WB
Kim D, Lee Y, Kim HR Et al. Hypothalamic administration of sargahydroquinoic acid elevates peripheral thermogenic signaling and ameliorates high fat diet-induced obesity through the sympathetic nervous system Scientific reports Oct 29 2021 [PMID: 34716371] (WB, Mouse) WB Mouse
Show All 184 Publications.

Reviews for PGC1 alpha Antibody (NBP1-04676) (11) 4.611

Average Rating: 4.6
(Based on 11 reviews)
We have 11 reviews tested in 6 species: Human, Mouse, Rat, Human and Mouse, Mouse and mus musculus, Other.

Reviews using NBP1-04676:
Filter by Applications
WB
(10)
ChIP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Rat
(3)
Human and Mouse
(1)
Mouse and mus musculus
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Robin Ren
WB Human and Mouse 10/30/2018
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate, Sample Amount: 50ug-100ug protein
SpeciesHuman and Mouse
LotG-8
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Human 12/14/2017
View

Summary

ApplicationWestern Blot
Sample Tested3 human cancer cell lines
SpeciesHuman
LotF-1

Comments

CommentsAB DILUTIION: 1/1000
Western Blot PGC1 alpha NBP1-04676
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 12/08/2017
View

Summary

ApplicationWestern Blot
Sample TestedPrimary mouse hepatocytes
SpeciesMouse
LotG-1

Comments

Commentsmouse hepatocytes were treated with 100 ng/ml LPS for 4 hours
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Francesca Pacifici
WB Mouse 09/26/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotF-2

Comments

CommentsWestern Blot: PGC1 alpha Antibody [NBP1-04676] - Total protein from murine skeletal muscle tissue, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PGC1-alpha 1:4000 in non-fat milk.
  5
reviewed by:
Verified Customer
ChIP Rat 04/20/2017
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedAdult spinal cord
SpeciesRat
LotE-1
  4
reviewed by:
Harini Sampath
WB Mouse and mus musculus 04/18/2017
View

Summary

ApplicationWestern Blot
Sample Testedmouse liver lysate
SpeciesMouse and mus musculus
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Green Waggener
WB Mouse 03/22/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotG1

Comments

CommentsC57BL/6 Mice
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Rat 11/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedRat Liver Nuclear Protein Extract
SpeciesRat
LotD-1

Blocking

Blocking Details1x iBind Solution (Invitrogen) in iBind Western Device at room temperature

Primary Anitbody

Dilution Ratioused at 0.5ug/mL (dilution = 1:2000 in 1x iBind Solution) in iBind Western Device at room temperature

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#NEF812001EA
Secondary Concentration1:5000

Details

Detection NotesPerkinElmer Western Lightning® Plus-ECL Enhanced Chemiluminescence Substrate, 1-second exposure

Comments

CommentsWe have used other PGC-1 alpha antibodies, but this one worked best!
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Rat 09/16/2015
View

Summary

ApplicationWestern Blot
Sample Testedrat spinal cord
SpeciesRat
LotD-1

Blocking

Blocking Details5% milk-PBST, 1hour, room temperature

Primary Anitbody

Dilution Ratio1:2000, overnight, 4d.c, 2% BSA-PBS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2004
Secondary Concentration1:4000

Details

Detection NotesECL, BioRad, 50sec.
Western Blot PGC1 alpha NBP1-04676
Enlarge
5
reviewed by:
Verified Customer
WB Other 04/08/2015
View

Summary

ApplicationWestern Blot
SpeciesOther
Special ApplicationsI recently purchased a PGC1alpha antibody (NBP1-04676ss) for my study and was told if it worked in my animal model (Siberian Hamster) that I am eligible for a 100% credit voucher. Attached is the western blot images of white adipose tissue that was acquired using Novus' PGC1alpha antibody.
FileView PDF
Western Blot PGC1 alpha NBP1-04676
Enlarge
3
reviewed by:
Giulia Ambrosi
WB Human 04/23/2013
View

Summary

ApplicationWestern Blot
Sample Testedrat liver homogenates and human fibroblasts lysate
SpeciesHuman
LotA-3
CommentsConsidering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Blocking

Blocking DetailsLeft membrane: Odyssey (Li-Cor) blocking buffer. Right membrane: 5% Milk in PBS. Both 1hour at room temperature

Primary Anitbody

Dilution Ratiodilution (1:2000) in blocking buffer (either Odyssey block buffer or PBS+5%Milk) +0.1% Tween-20, overnight incubation at 4°C

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IRDye 680, Odyssey Li-Cor
Secondary Manufacturer Cat#926-32223
Secondary Concentration1:10000

Details

Detection NotesImage analysis with a scanner from Odyssey near infrared technology. Scanner intensity: 5

Comments

CommentsConsidering our experience, the antibody PGC1-alpha (NBP1-04676) from Novus Biologicals works very well in western blot. It can be used at relatively high dilutions, it can be frozen and re-used with no loss of signal intensity and finally it can be adopted on protein samples derived from different species (i.e. human and rat tested) with approximately the same detection power. The antibody is sensitive and specific: a clear band appears at the expected molecular weight (~90Kda) and the background noise is very little, especially on rodent protein extracts.

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PGC1 alpha Antibody (NBP1-04676). (Showing 1 - 4 of 4 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. What is the second band in the image from verified customer review: Western Blot: PGC1 alpha Antibody [NBP1-04676] - PGC1 alpha expression in lysates from Siberian hamster using anti-PGC1 alpha antibody?
    • The second band in the customer review image on our datasheet is from their actin loading control. Our band just below 50 kDa in human kidney lysate has not yet been identified. It is possible that it was heavy chain IgG detection from the secondary
  3. Do you have any information about the specificity of NBP1-04676 with the methylated form of PGC1 alpha?
    • According to UniProt, there are no methylated a.a. reported for this protein target.  However there are 13 N6-acetyllysine reported, and a.a. 413, 442, and 451 are included in the immunogen.  Since this is a polyclonal antibody, it's anticipated that this antibody will detect both unacetylated and acetylated PGC1 alpha, regardless of where or how many of a.a. (lysine) residues are modified.
  4. I am looking for a primary PGC1 alpha antibody against human to be used in fluorescence. However, it cannot be conjugated with FITC (Green). It has to be conjugated with something else (Gold, red, orange, blue or aqua). Do you have such a product? 
    • NBP1-04676R is conjugated to DyLight 550 which is an equivalent of TRITC. NBP1-04676C is conjugated to DyLight 650 which is an equivalent of APC. One of these two should fit your needs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on PGC1 alpha.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

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Recent Reviews

4.6
5
8
4
2
3
1
2
0
1
0

Robin Ren
10/30/2018
Application: WB
Species: Human and Mouse

Verified Customer
12/14/2017
Application: WB
Species: Human

Verified Customer
12/08/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol PPARGC1A
Entrez
Uniprot