FOXO3 Antibody

Images

 
Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody [NBP2-16521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:1000. Blue: ...read more
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse duodenum. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Chromatin Immunoprecipitation (ChIP): FOXO3 Antibody [NBP2-16521] - (D) ChIP assay for LMOD1 and EGFR (as a positive control region) in HCASMC chromatin lysates immunoprecipitated with antibodies to FOXO3 or a negative ...read more
Western Blot: FOXO3 Antibody [NBP2-16521] - 50 ug of mouse brain whole cell lysate). Antibody at 1:500.
Genetic Strategies: Western Blot: FOXO3 Antibody [NBP2-16521] - Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A ...read more
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat colon. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse testis. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Rat brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Mouse brain. FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minutes.
Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - Jurkat whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ug of FOXO3A antibody 5% SDS-PAGE The ...read more
LMOD1 expression is mediated by PDGF-BB-FOXO3 signaling cascade. Western blotting (C) data revealing PDGF-BB and phosphorylated-FOXO3 (P-FOXO3) mediated LMOD1 expression. Image collected and cropped by CiteAb from the ...read more
Specificity of antibodies for tryptophan 2,3-dioxygenase (TDO) by Western blot (A) and immunofluorescent staining (B). A: The total protein (20 μg) of the dentate gyrus, extracted from the wild-type (lanes 1 and 3) and ...read more
ATO interferes with HAdV gene expression and capsid formation. H1299 cells were infected with HAdV‐C5 wt at a multiplicity of 20 FFU per cell and treated with the depicted concentrations of ATO at 2 h p.i. A) Cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Sq, Xp, Po, Bv, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

FOXO3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary.
Localization
Cytoplasm, Cytosol, Nucleus
Predicted Species
Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Application Notes
ChIP usage reported in scientific literature (PMID: 26229077).
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 4 Reviews rated 4
using
NBP2-16521 in the following applications:

Publications
Read Publications using
NBP2-16521 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7).
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FOXO3 Antibody

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for FOXO3 Antibody (NBP2-16521)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ChIP, Chemotaxis, IF/IHC, IP, WB.


Filter By Application
ChIP
(1)
Chemotaxis
(3)
IF/IHC
(2)
IP
(2)
WB
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP2-16521 Applications Species
Qiu CW, Chen B, Zhu HF et al. Gastrodin alleviates cisplatin nephrotoxicity by inhibiting ferroptosis via the SIRT1/FOXO3A/GPX4 signaling pathway Journal of ethnopharmacology 2023-10-05 [PMID: 37802374] (IP) IP
Yan D, He Q, Pei L et al. The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer Cell Death & Disease 2023-08-12 [PMID: 37573356]
Xing X, Liu M, Wang X et al. Promoting effects of calponin 3 on the growth of diffuse large B?cell lymphoma cells Oncology reports 2023-02-01 [PMID: 36660952] (ChIP, Human) ChIP Human
Niu J, Yan T, Guo W et al. The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcoma Autophagy 2022-11-30 [PMID: 36451342] (Chemotaxis, Human) Chemotaxis Human
Lan J, Wei G, Liu J et al. Chemotherapy-induced adenosine A2B receptor expression mediates epigenetic regulation of pluripotency factors and promotes breast cancer stemness Theranostics 2022-02-28 [PMID: 35401817] (IP, Human) IP Human
Park M, Baker W, Cambow D et al. Methamphetamine Enhances HIV-Induced Aberrant Proliferation of Neural Progenitor Cells via the FOXO3-Mediated Mechanism Molecular Neurobiology 2021-11-01 [PMID: 33983546]
Wang JJ, Ge W, Zhai QY et al. Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice PLoS biology 2020-12-22 [PMID: 33351795] (IF/IHC, Mouse) IF/IHC Mouse
Yan, S;Wang, Q;Huo, Z;Yang, T;Yin, X;Wang, Z;Zhang, Z;Wu, H; Gene expression profiles between cystic and solid vestibular schwannoma indicate susceptible molecules and pathways in the cystic formation of vestibular schwannoma Funct. Integr. Genomics 2019-04-05 [PMID: 30953268] (IF/IHC, Human) IF/IHC Human
Nanda V, Wang T, Pjanic M et al. Functional regulatory mechanism of smooth muscle cell-restricted LMOD1 coronary artery disease locus. PLoS Genet. 2018-11-16 [PMID: 30444878] (Chemotaxis, Human) Chemotaxis Human
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015-07-30 [PMID: 26229077] (Chemotaxis, WB, Human) Chemotaxis, WB Human
Show All 11 Publications.

Reviews for FOXO3 Antibody (NBP2-16521) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-16521:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(3)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FOXO3 NBP2-16521
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedNuclear fraction of human breast cancer cell lines
SpeciesHuman
Lot39443
Western Blot FOXO3 NBP2-16521
Enlarge
4
reviewed by:
Verified Customer
WB Human 09/18/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman Cancer Cells
SpeciesHuman
Lot39443
Western Blot FOXO3 NBP2-16521
Enlarge
4
reviewed by:
Verified Customer
WB Mouse 03/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
Western Blot FOXO3 NBP2-16521
Enlarge
4
reviewed by:
Verified Customer
WB Human 07/31/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FOXO3 Antibody (NBP2-16521) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
4
3
0
2
0
1
0

Verified Customer
10/24/2018
Application: WB
Species: Human

Verified Customer
09/18/2018
Application: WB
Species: Human

Verified Customer
03/13/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol FOXO3
Uniprot