FOXO3 Antibody

Images

 
Western Blot: FOXO3 Antibody [NBP2-16521] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the ...read more
Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody [NBP2-16521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FOXO3A protein stained by FOXO3A antibody [C3], C-term diluted at 1:500. Red: ...read more
Immunohistochemistry-Paraffin: FOXO3A Antibody [NBP2-16521] - Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using antibody at 1:500 dilution.
Western Blot: FOXO3A Antibody [NBP2-16521] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:500.
Western Blot: FOXO3A Antibody [NBP2-16521] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:500.
Western Blot: FOXO3 Antibody [NBP2-16521] - Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A antibody [C3], C-term.
Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of FOXO3A protein by 2 ug of FOXO3A antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, ShSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml
     

Genetic Strategies

   

Order Details

FOXO3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FOXO3A. The exact sequence is proprietary.
Localization
Cytoplasm; cytosol; Nucleus
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
ChIP usage reported in scientific literature (PMID: 26229077).
Theoretical MW
71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4
using
NBP2-16521 in the following applications:

Publications
Read Publications using
NBP2-16521 in the following applications:

  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FOXO3 Antibody

  • AF6q21 protein
  • AF6q21
  • DKFZp781A0677
  • FKHRL1
  • FKHRL1MGC31925
  • FKHRL1P2
  • forkhead box O3
  • forkhead box protein O3
  • forkhead homolog (rhabdomyosarcoma) like 1
  • Forkhead in rhabdomyosarcoma-like 1
  • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • FOXO2
  • FoxO3
  • FOXO3A
  • FOXO3Aforkhead box O3A
  • MGC12739

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FOXO3 Antibody (NBP2-16521)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ChIP, WB.


Filter By Application
ChIP
(1)
WB
(2)
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Filter By Species
Human
(1)
Mouse
(1)
All Species

Reviews for FOXO3 Antibody (NBP2-16521) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP2-16521:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot FOXO3 NBP2-16521
Enlarge
reviewed by:
Anonymous
WB Mouse 03/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
Western Blot FOXO3 NBP2-16521
Enlarge
reviewed by:
Anonymous
WB Human 07/31/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FOXO3 Antibody (NBP2-16521) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FOXO3 Antibody (NBP2-16521)

Discover related pathways, diseases and genes to FOXO3 Antibody (NBP2-16521). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for FOXO3 Antibody (NBP2-16521)

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Blogs on FOXO3

There are no specific blogs for FOXO3, but you can read our latest blog posts.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Anonymous
03/13/2017
Application: WB
Species: Mouse

Anonymous
07/31/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOXO3
Entrez
Uniprot