Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: FOXO3 Antibody [NBP2-16521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:1000. Blue: ...read more
Western Blot: FOXO3 Antibody [NBP2-16521] - 30 ug of whole 293T cell lysate. Antibody at 1:500.
Western Blot: FOXO3 Antibody [NBP2-16521] - 50 ug of mouse brain whole cell lysate). Antibody at 1:500.
Genetic Strategies: Western Blot: FOXO3 Antibody [NBP2-16521] - Non-transfected (-) and transfected (+) C2C12 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXO3A ...read more
Western Blot: FOXO3 Antibody [NBP2-16521] - 30 ug of whole cell lysate. A: Jurkat. B: Raji. Antibody at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunoprecipitation: FOXO3 Antibody [NBP2-16521] - A. Control with 2 ug of preimmune rabbit IgG B. IP of FOXO3A protein by 2 ug of FOXO3A antibody at 1:1000.
ChIP usage reported in scientific literature (PMID: 26229077).
71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS, 20% Glycerol (pH7).
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for FOXO3 Antibody
forkhead box O3
forkhead box protein O3
forkhead homolog (rhabdomyosarcoma) like 1
Forkhead in rhabdomyosarcoma-like 1
forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
FOXO3Aforkhead box O3A
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for FOXO3 Antibody (NBP2-16521)(4)
We have publications tested in 2 confirmed species: Human, Mouse.
We have publications tested in 3 applications: ChIP, IHC, WB.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.