FoxO1/FKHR Antibody (83N7F8)

Images

 
Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Western blot analysis of Ntera2 cell lysate (A), HEK293 cell lysate (B), and human placenta tissue (C) using FOXO1A antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - IHC-P analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells ...read more
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a ...read more
Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - WB detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a ...read more
Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - IHC-P analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and ...read more
Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary ...read more
Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Simple Western lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, SqSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
83N7F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FoxO1/FKHR Antibody (83N7F8) Summary

Immunogen
FOXO1A
Localization
Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus.
Predicted Species
Mouse (92%), Rat (92%), Porcine (90%), Squirrel (91%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FOXO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-31376 in the following applications:

Reactivity Notes

Human. Based upon immunogen sequence simlarity, this antibody is predicted to react with Mouse (92%), Rat (92%), Bovine/Cow (91%), Squirrel (91%) and Porcine/Pig (90%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for FoxO1/FKHR Antibody (83N7F8)

  • FKH1
  • FKHR
  • FKHRforkhead box protein O1
  • forkhead box O1
  • Forkhead box protein O1A
  • forkhead homolog in rhabdomyosarcoma
  • Forkhead in rhabdomyosarcoma
  • FoxO1
  • FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

The FOX1A transcription factor is a member of forkhead family of transcription factors. It is the main target of insulin signaling. When insulin is present, its activity is suppressed. It regulates metabolic homeostasis in response to oxidative stress. It has several other functions: main regulator of redox balance, control osteoblast numbers, promotes gluconeogenesis (hepatocytes), cell death regulation, promote neural cell death, regulation of adipogenic genes, regulate transcriptional activity of GADD45A, and repair nitric oxide-damaged DNA (beta-cells).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxO1/FKHR Antibody (NBP2-31376) (0)

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Review for FoxO1/FKHR Antibody (NBP2-31376) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-31376:
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Images Ratings Applications Species Date Details
Western Blot FoxO1/FKHR NBP2-31376
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reviewed by:
Anonymous
WB Human 08/23/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxO1/FKHR Antibody (NBP2-31376) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FoxO1/FKHR Antibody (NBP2-31376)

Discover related pathways, diseases and genes to FoxO1/FKHR Antibody (NBP2-31376). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FoxO1/FKHR Antibody (NBP2-31376)

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Pathways for FoxO1/FKHR Antibody (NBP2-31376)

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PTMs for FoxO1/FKHR Antibody (NBP2-31376)

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Research Areas for FoxO1/FKHR Antibody (NBP2-31376)

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Blogs on FoxO1/FKHR.

FOXO1/FKHR (fork head in Rhabdomyosarcoma)
FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,...  Read full blog post.

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Recent Reviews

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2
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1
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Anonymous
08/23/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol FOXO1
Entrez
Uniprot