FoxO1/FKHR Antibody (83N7F8)

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.

Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.

Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Rt, Mu, Rt, Po, Bv, Sq
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
• Clone: 83N7F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FoxO1/FKHR Antibody (83N7F8) Summary

• Immunogen: FOXO1A
• Localization: Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus.
• Predicted Species: Mouse (92%), Rat (92%), Porcine (90%), Bovine (91%), Squirrel (91%). Backed by our 100% Guarantee.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FOXO1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 0.1-2 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31553623).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for FoxO1/FKHR Antibody (83N7F8)

• FKH1
• FKHR
• FKHRforkhead box protein O1
• forkhead box O1
• Forkhead box protein O1A
• forkhead homolog in rhabdomyosarcoma
• Forkhead in rhabdomyosarcoma
• FoxO1
• FOXO1Aforkhead, Drosophila, homolog of, in rhabdomyosarcoma

Background

The FOX1A transcription factor is a member of forkhead family of transcription factors. It is the main target of insulin signaling. When insulin is present, its activity is suppressed. It regulates metabolic homeostasis in response to oxidative stress. It has several other functions: main regulator of redox balance, control osteoblast numbers, promotes gluconeogenesis (hepatocytes), cell death regulation, promote neural cell death, regulation of adipogenic genes, regulate transcriptional activity of GADD45A, and repair nitric oxide-damaged DNA (beta-cells).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxO1/FKHR Antibody (NBP2-31376)(2)

Publications using NBP2-31376

• Presby DM, Checkley LA, Jackman MR et al. Regular exercise potentiates energetically expensive hepatic de novo lipogenesis during early weight regain Am. J. Physiol. Regul. Integr. Comp. Physiol. Sep 25 2019 [PMID: 31553623] (WB, Rat)
• Hepp P, Hutter S, Knabl J et al. Histone H3 lysine 9 acetylation is downregulated in GDM Placentas and Calcitriol supplementation enhanced this effect Int J Mol Sci Dec 14 2018 [PMID: 30558244] (WB, Human)

Review for FoxO1/FKHR Antibody (NBP2-31376) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 08/23/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Blocking

• Blocking Details: Blocking with 5% non-fat milk for 30 min

Primary Anitbody

• Dilution Ratio: 1:1000, overnight

Secondary Antibody

• Secondary Description: Sheep anti-mouse IgG, HRP
• Secondary Manufacturer Cat#: GE Healthcare, NA931V
• Secondary Concentration: 1:2000

Details

• Detection Notes: Regular Western blot Protocol

Bioinformatics

• Gene Symbol: FOXO1
• Entrez:
  • 2308(Human)
• Uniprot:
  • Q12778(Human)