![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Western-Blot-NBP2-31376-img0007.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of (A) Ntera2 cell lysate, (B) HEK293 cell lysate, and (C) human placenta tissue using FOXO1A antibody at a concentration of 2 ug/ml.")
| Reactivity | Hu, Mu, Rt, Po, Bv, SqSpecies Glossary |
| Applications | WB, Simple Western, Flow, ICC/IF, IHC, IHC-P |
| Clone | 83N7F8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | FOXO1A |
| Localization | Cytoplasm. Nucleus. Note: Shuttles between the cytoplasm and nucleus. |
| Predicted Species | Mouse (92%), Rat (92%), Porcine (90%), Bovine (91%), Squirrel (91%). Backed by our 100% Guarantee. |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | FOXO1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Anonymous |
WB | Human | 08/23/2015 |
Summary
Blocking
Primary Anitbody
Secondary Antibody
Details
|
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-31376AF350 | |
| Alexa Fluor 405 | NBP2-31376AF405 | |
| Alexa Fluor 488 | NBP2-31376AF488 | |
| Alexa Fluor 532 | NBP2-31376AF532 | |
| Alexa Fluor 594 | NBP2-31376AF594 | |
| Alexa Fluor 647 | NBP2-31376AF647 | |
| Alexa Fluor 700 | NBP2-31376AF700 | |
| Alexa Fluor 750 | NBP2-31376AF750 | |
| Biotin | NBP2-31376B | |
| DyLight 350 | NBP2-31376UV | |
| DyLight 405 | NBP2-31376V | |
| DyLight 488 | NBP2-31376G | |
| DyLight 550 | NBP2-31376R | |
| DyLight 594 | NBP2-31376DL594 | |
| DyLight 650 | NBP2-31376C | |
| DyLight 680 | NBP2-31376FR | |
| DyLight 755 | NBP2-31376IR | |
| FITC | NBP2-31376F | |
| HRP | NBP2-31376H | |
| Janelia Fluor 549 | NBP2-31376JF549 | |
| Janelia Fluor 646 | NBP2-31376JF646 |
Diseases for FoxO1/FKHR Antibody (NBP2-31376)Discover more about diseases related to FoxO1/FKHR Antibody (NBP2-31376).
| Pathways for FoxO1/FKHR Antibody (NBP2-31376)View related products by pathway.
|
|
FOXO1/FKHR (fork head in Rhabdomyosarcoma) FOXO1 belongs to the very large Forkhead family of transcription factors which contain a conserved distinct DNA-binding domain known as the Forkhead Box, or FOX. The Forkhead domain is a 100 amino acid long motif capable of binding and bending DNA,... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Immunocytochemistry-Immunofluorescence-NBP2-31376-img0009.jpg "Immunocytochemistry/Immunofluorescence: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - FOXO1A antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). An antibody concentration of 0.1 ug/ml was used. Image objective 40x.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Immunohistochemistry-Paraffin-NBP2-31376-img0011.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human uterus by using FOXO1A antibody at a concentration of 5 ug/ml. The columnar cells of straight non-convoluted glands in uterous showed specific staining for FOXO1A in the cellular cytoplasm as well as nuclei, whereas the stromal cells showed mild cytoplasmic staining only.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Flow-Cytometry-NBP2-31376-img0016.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on RH-30 cells with FoxO1/FKHR Antibody [83N7F8] NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Western-Blot-NBP2-31376-img0004.jpg "Western Blot: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Detection of FOXO1A / FOXO1 protein in (A) Hela cells lysate, (B) Jurkat cell lysate, and (C) partial recombinant protein by using FOXO1A antibody at a concentration of 0.5 ug/ml for lysate and 0.05 ug/ml for the recombinant protein.")
![Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Immunohistochemistry-Paraffin-NBP2-31376-img0010.jpg "Immunohistochemistry-Paraffin: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis of FOXO1A protein in a section of normal human kidney by using FOXO1A antibody at a concentration of 5 ug/ml. Cytoplasmic and nuclear positivity was observed in the cells of various tubules/ducts of renal medulla.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Flow-Cytometry-NBP2-31376-img0013.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Analysis using the Alexa Fluor 488 conjugated FoxO1 antibody, NBP2-31376AF488 (blue) and fluorescence minus one control (red). Staining of FoxO1 in primary CD4+ T-cells isolated from human peripheral blood using 1uL of antibody per 1X10e5 cells. Image from verified customer review.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Flow-Cytometry-NBP2-31376-img0014.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Flow-Cytometry-NBP2-31376-img0015.jpg "Flow Cytometry: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - An intracellular stain was performed on U2-OS cells with FoxO1/FKHR [83N7F8] Antibody NBP2-31376AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/FoxO1-FKHR-Antibody-(83N7F8)-Simple-Western-NBP2-31376-img0012.jpg "Simple Western: FoxO1/FKHR Antibody (83N7F8) [NBP2-31376] - Lane view shows a specific band for FoxO1/FKHR in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.")
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.](http://images.novusbio.com/fullsize2/AKT1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0014.jpg)
![Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize2/AKT1-[p-Ser473]-Antibody-(104A282)-Western-Blot-NB100-56749-img0015.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/AKT1-[p-Ser473]-Antibody-(104A282)-Immunohistochemistry-Paraffin-NB100-56749-img0009.jpg)
![Western Blot: FOXO3 Antibody [NBP2-16521] - FoxO3 expression in nuclear and cytosolic fraction of human breast cancer cell line MDA-MB-231. Image from verified customer review.](http://images.novusbio.com/fullsize2/FOXO3-Antibody-Western-Blot-NBP2-16521-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: FOXO3 Antibody [NBP2-16521] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FOXO3A stained by FOXO3A antibody [C3], C-term diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/FOXO3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16521-img0023.jpg)
![Immunohistochemistry-Paraffin: FOXO3 Antibody [NBP2-16521] - Huh-7 xenograft, using FOXO3A antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize2/FOXO3A Antibody-Immunohistochemistry-Paraffin-NBP2-16521-img0003.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0004.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of PYK2 on mouse brain lysates using anti-PYK2 antibody at 1:1000 dilution.](http://images.novusbio.com/fullsize2/PYK2-FAK2 Antibody (SC06-15)-Western Blot-NBP2-67627-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Staining PYK2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilized with 0.25% Triton X-100/PBS.](http://images.novusbio.com/fullsize2/PYK2-FAK2-Antibody-(SC06-15)-Immunocytochemistry-Immunofluorescence-NBP2-67627-img0003.jpg)
![Immunohistochemistry-Paraffin: PYK2/FAK2 Antibody (SC06-15) [NBP2-67627] - Analysis of paraffin-embedded mouse kidney tissue using anti-PYK2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PYK2-FAK2-Antibody-(SC06-15)-Immunohistochemistry-Paraffin-NBP2-67627-img0008.jpg)
![Western Blot: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PI 3-Kinase p110.](http://images.novusbio.com/fullsize2/PI 3-Kinase p110 gamma Antibody (2B1)-Western Blot-NBP2-46411-img0010.jpg)
![Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PI-3-Kinase-p110-gamma-Antibody-(2B1)-Immunohistochemistry-NBP2-46411-img0009.jpg)
![Immunohistochemistry: PI 3-Kinase p110 gamma Antibody (2B1) [NBP2-46411] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize2/PI-3-Kinase-p110-gamma-Antibody-(2B1)-Immunohistochemistry-NBP2-46411-img0001.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize2/PIK3CA-Antibody-(6D9)-Immunohistochemistry-NBP2-46403-img0011.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize2/PIK3CA-Antibody-(6D9)-Immunohistochemistry-NBP2-46403-img0001.jpg)
![Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize2/PIK3CA-Antibody-(6D9)-Immunohistochemistry-NBP2-46403-img0002.jpg)
![Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - A. 30 ug PC-12 whole cell lysate/extract 5 % SDS-PAGE PI3K p110 beta antibody [N3C2], Internal dilution: 1:1000](http://images.novusbio.com/fullsize2/PI-3-Kinase-p110-beta-PIK3CB-Antibody-Western-Blot-NBP1-33116-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/PI-3-Kinase-p110-beta-PIK3CB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33116-img0005.jpg)
![Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody [NBP1-33116] - Paraffin-embedded rat testis. PI3K p110 beta antibody [N3C2], Internal diluted at 1:400.](http://images.novusbio.com/fullsize2/PI-3-Kinase-p110-beta-PIK3CB-Antibody-Immunohistochemistry-Paraffin-NBP1-33116-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Staining FOXO4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/FOXO4-Antibody-(JJ09-11)-Immunocytochemistry-Immunofluorescence-NBP2-67892-img0003.jpg)
![Flow Cytometry: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Analysis of 293 cells with FOXO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize2/FOXO4-Antibody-(JJ09-11)-Flow-Cytometry-NBP2-67892-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FOXO4 Antibody (JJ09-11) [NBP2-67892] - Staining FOXO4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/FOXO4-Antibody-(JJ09-11)-Immunocytochemistry-Immunofluorescence-NBP2-67892-img0002.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Sirtuin-1-SIRT1-Antibody-(1F3)-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Western Blot: G6PC Antibody [NBP1-80533] - Sample Tissue: Mouse Lung Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize2/G6PC-Antibody-Western-Blot-NBP1-80533-img0003.jpg)
![Western Blot: G6PC Antibody [NBP1-80533] - Mouse Intestine, concentration 1 ug/ml.](http://images.novusbio.com/fullsize2/G6PC-Antibody-Western-Blot-NBP1-80533-img0002.jpg)
![Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize2/TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0004.jpg)
![Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.](http://images.novusbio.com/fullsize2/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-Paraffin-NB600-607-img0005.jpg)
![Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize2/TOR-mTOR-[p-Ser2448]-Antibody-Immunohistochemistry-NB600-607-img0003.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), BIM NBP1-77118, (5 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize2/Bim-Antibody-Western-Blot-NBP1-76963-img0012.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP1-76963] - Human skin cancer cells with Bim antibody at 20 ug/ml.](http://images.novusbio.com/fullsize2/Bim-Antibody-Immunohistochemistry-Paraffin-NBP1-76963-img0001.jpg)
![Western Blot: Bim Antibody [NBP1-76963] - Western Blot Validation in Rat Myeloma Cell line:Loading: 15 ug of rat myeloma YB2/0 cell lysate per lane.Antibodies: BIM NBP1-76963, (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize2/Bim-Antibody-Western-Blot-NBP1-76963-img0008.jpg)
![Immunohistochemistry-Paraffin: Insulin Receptor beta Antibody [NBP2-12793] - Staining shows expression of insulin receptor beta in the Cortex in mouse brain. Dilution 1:500](http://images.novusbio.com/fullsize2/Insulin-Receptor-beta-Antibody-Immunohistochemistry-Paraffin-NBP2-12793-img0002.jpg)
![Immunoprecipitation: Insulin Receptor beta Antibody [NBP2-12793] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Insulin Receptor Beta antibody NBP2-12793 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Insulin Receptor Beta was also immunoprecipitated by rabbit anti-Insulin Receptor Beta antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize2/Insulin-Receptor-beta-Antibody-Immunoprecipitation-NBP2-12793-img0001.jpg)
