Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.

Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining. Image from the standard format of this antibody.

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.

Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.

• Reactivity: Hu, Mu, Rt, Pm, Rb
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, IHC
• Clone: 1F3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free Summary

• Immunogen: Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT1
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:1000
  • Immunohistochemistry Free-Floating reported in scientific literature (PMID 26327687)
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 120 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

SIRT1, the human homolog of the S. cerevisiae SIR2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT1