Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mouse mAb against PANC-1 (1) cell lysate.

Immunohistochemistry Whole-Mount: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Cochlear whole mount from wild-type and Sirt3-KO mice, immunolabeled with MYO7, SIRT3, and DAPI. The cross-hairs show the position from which the side view is taken. SIRT3 immunoreactivity only is shown in the right panels. IHC-WhMt image submitted by a verified customer review.

Flow Cytometry: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis of HeLa cells using SIRT3 mouse mAb (green) and negative control (red).

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against HEK293 (1) and SIRT3 (AA: 155-290)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)

ELISA: Sirtuin 3/SIRT3 Antibody (6B2A1) [NBP2-52563] - Black line: Control Antigen (100 ng); Purple line: Antigen(10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF, IHC
• Clone: 6B2A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Sirtuin 3/SIRT3 (AA: 155-290) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SIRT3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry 1:200 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready. This Sirtuin 3/SIRT3 Antibody (6B2A1) is validated for IHC-WhMt from a verified customer review.
• Theoretical MW: 43.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Sirtuin 3/SIRT3 Antibody (6B2A1) - BSA Free

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

SIRT3 is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sirtuin 3/SIRT3 Antibody (NBP2-52563)(1)

Publication using NBP2-52563

• Patel S, Shah L, Dang N, Tan X et Al. SIRT3 promotes auditory function in young adult FVB/nJ mice but is dispensable for hearing recovery after noise exposure PLoS One 2020-07-14 [PMID: 32658908]

Review for Sirtuin 3/SIRT3 Antibody (NBP2-52563) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Immunofluorescence histochemistry Mouse 01/02/2020

Summary

• Application: Other
• Sample Tested: Cochlear whole mount,mouse cochlear whole mount
• Species: Mouse
• Lot: 150630

Bioinformatics

• Gene Symbol: SIRT3