RelA/NFkB p65 Antibody

Images

 
Western Blot: RelA/NFkB p65 Antibody [NB100-2176] - Analysis of NFkB p65 expression in A431 (A) and HeLa (B) whole cell lysates.
Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody [NB100-2176] - Staining of formalin-fixed, paraffin-embedded human DLBCL showing nuclear expression of NFkB p65 in the tumor cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

RelA/NFkB p65 Antibody Summary

Immunogen
Synthetic peptide corresponding to the C-terminus of human NFkB p65.
Localization
Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B).
Specificity
Reacts with NFkB p65
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RELA
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:400-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
In Western Blot this antibody detects a band of approximately 64 kDa (predicted molecular weight: 69 kDa). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27119753).
Positive Control
K-562 Lysate (NB820-59464)
NIH 3T3 Lysate (NB800-PC8)
A-431 Lysate (NB820-59461)
NFkB p105/p50 Lysate (NBL1-13619)
Reviewed Applications
Read 1 Review rated 5
using
NB100-2176 in the following applications:

Publications
Read Publications using
NB100-2176 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, 100 ug BSA.
Preservative
0.1% Sodium Azide
Concentration
0.2 mg/ml
Purity
IgG purified

Alternate Names for RelA/NFkB p65 Antibody

  • MGC131774
  • nf kb p65
  • NFkB p65
  • NF-kB p65
  • NFKB3
  • NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
  • Nuclear factor NF-kappa-B p65 subunit
  • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
  • p65
  • p65RelA
  • rela p65
  • RelA
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65

Background

p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RelA/NFkB p65 Antibody (NB100-2176)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(2)
WB
(4)
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Human
(2)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB100-2176 Applications Species
Ribes D, Casemayou A, El Hachem H et al. Asymptomatic circulating T-cell clone cause renal polymorphic inflammatory fibrosis. Clin. Exp. Nephrol. Dec 26 2016 [PMID: 28018996] (WB, Human) WB Human
Liu JY, Guo F, Wu HL et al. Midazolam anesthesia protects neuronal cells from oxidative stress-induced death via activation of the JNK-ERK pathway Mol Med Rep Jan 1 2017 [PMID: 27959401] (WB, Mouse) WB Mouse
Li J, Luco AL, Ochietti B et al. Tumoral Vitamin D Synthesis by CYP27B1 1-alpha-Hydroxylase Delays Mammary Tumor Progression in the PyMT-MMTV Mouse Model and Its Action Involves NF-kB Modulation. Endocrinology. Apr 27 2016 [PMID: 27119753] (ICC/IF, Mouse) ICC/IF Mouse
Sahbaz A, Aynioglu O, Isik H et al. Cholecalciferol (vitamin D3) prevents postoperative adhesion formation by inactivating the nuclear factor kappa B pathway: a randomized experimental study. J. Surg. Res. 2015 May 14 [PMID: 26038248] (IHC-P, Rat) IHC-P Rat
Zhu Shijun, Moore Terry W, Morii Nao et al. Synthetic curcumin analog UBS109 inhibits the growth of head and neck squamous cell carcinoma xenografts. Curr Cancer Drug Targets. 2014 May 01 [PMID: 24628271]
Zaky A, Mohammad B, Moftah M et al. Apurinic/apyrimidinic endonuclease 1 is a key modulator of aluminum-induced neuroinflammation. BMC Neurosci 2013 Mar 19 [PMID: 23497276] (WB, Rat) WB Rat
Zhu S, Moore TW, Lin X et al. Synthetic curcumin analog EF31 inhibits the growth of head and neck squamous cell carcinoma xenografts. Integr Biol (Camb). 2012 Jun [PMID: 22532032] (IHC-P, WB, Human) IHC-P, WB Human
Kang HB, Kim YE, Kwon HJ, Sok DE, Lee Y. Enhancement of NF-kappaB expression and activity upon differentiation of human embryonic stem cell line SNUhES3. Stem Cells Dev;16(4):615-23. 2007 Aug. [PMID: 17784835]
Fan Z, Soder S, Oehler S, Fundel K, Aigner T. Activation of interleukin-1 signaling cascades in normal and osteoarthritic articular cartilage. Am J Pathol;171(3):938-46. 2007 Sep. [PMID: 17640966]
Longpre F, Garneau P, Christen Y, Ramassamy C. Protection by EGb 761 against beta-amyloid-induced neurotoxicity: involvement of NF-kappaB, SIRT1, and MAPKs pathways and inhibition of amyloid fibril formation. Free Radic Biol Med;41(12):1781-94. 2006 Dec 15. [PMID: 17157181]
Show All 19 Publications.

Review for RelA/NFkB p65 Antibody (NB100-2176) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-2176:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot RelA/NFkB p65 NB100-2176
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reviewed by:
Anonymous
WB Human 08/31/2017
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Summary

ApplicationWestern Blot
Sample TestedEndothelial,Whole cell lysates
SpeciesHuman
LotΑ2816

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RelA/NFkB p65 Antibody (NB100-2176). (Showing 1 - 1 of 1 FAQs).

  1. I performed Western blot with NFkB p65 Antibody (NB100-2176) using brain and liver tissues extracts. My question: is it possible that NF-kB-Ikb complex could be detected by this antibody? Because I found 2 bands in the Western blot results, one is located at 65 and the other above 100 kD.
    • It possible that NB100-2176 could detect the entire NFkB complex in addition to just the p65 protein. If your samples were not completely reduced and denatured, they could have remained bound in your gel. The band above 100 kDa could also be an aggregate of NFkB. You may consider probing that same membrane for a different NFkB complex protein that you would expect to remain bound under these conditions. A negative signal in this case would tell you that it's an aggregate of NFkB, or maybe that it's a non-specific band. Did you run a negative control?

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional RelA/NFkB p65 Products

Bioinformatics Tool for RelA/NFkB p65 Antibody (NB100-2176)

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Diseases for RelA/NFkB p65 Antibody (NB100-2176)

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PTMs for RelA/NFkB p65 Antibody (NB100-2176)

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Blogs on RelA/NFkB p65.

The effect of antioxidants and the NFkB p65 pathway in inflammation
NFkB is a transcription factor that plays a role in the expression of genes involved in immune response, inflammation, metastasis, cell survival and more. RelA (p65) is one member of the NFkB mammalian family, alongside other subunits. NFkB subunit...  Read full blog post.

NFkB3-p65: Say that three times fast!
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus...  Read full blog post.

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Recent Reviews

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Anonymous
08/31/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol RELA
Entrez
Uniprot