In Western Blot this antibody detects a band of approximately 64 kDa (predicted molecular weight: 69 kDa). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 27119753).
Read Publications using NB100-2176 in the following applications:
NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
Nuclear factor NF-kappa-B p65 subunit
Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
v-rel reticuloendotheliosis viral oncogene homolog A (avian)
v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for RelA/NFkB p65 Antibody (NB100-2176). (Showing 1 - 1 of 1 FAQs).
I performed Western blot with NFkB p65 Antibody (NB100-2176) using brain and liver tissues extracts. My question: is it possible that NF-kB-Ikb complex could be detected by this antibody? Because I found 2 bands in the Western blot results, one is located at 65 and the other above 100 kD.
It possible that NB100-2176 could detect the entire NFkB complex in addition to just the p65 protein. If your samples were not completely reduced and denatured, they could have remained bound in your gel. The band above 100 kDa could also be an aggregate of NFkB. You may consider probing that same membrane for a different NFkB complex protein that you would expect to remain bound under these conditions. A negative signal in this case would tell you that it's an aggregate of NFkB, or maybe that it's a non-specific band. Did you run a negative control?
NFkB3-p65: Say that three times fast! NF-kappa-B is a ubiquitous transcription factor involved in several biological processes such as inflammation, immunity, differentiation, cell growth, tumor genesis and apoptosis. Unlike the majority of transcription factors that reside in the nucleus... Read full blog post.