UCP2 Antibody

Images

 
Western Blot: UCP2 Antibody [NB100-59742] - Staining of rat adipose (A) and mouse spleen (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence. Approx 37 kDa band observed in rat ...read more
Immunocytochemistry/ Immunofluorescence: UCP2 Antibody [NB100-59742] - Analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

UCP2 Antibody Summary

Immunogen
Peptide with sequence C-DSVKQFYTKGSEH corresponding to internal region according to NP_003346.2.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UCP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
UCP2 Overexpression Lysate
UCP2 Knockout HeLa Cell Lysate
UCP2 Knockout 293T Cell Lysate
Reviewed Applications
Read 2 Reviews rated 3
using
NB100-59742 in the following applications:

Publications
Read Publications using
NB100-59742 in the following applications:

Reactivity Notes

Predicted cross-reactivity based on sequence similarity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UCP2 Antibody

  • mitochondrial uncoupling protein 2
  • SLC25A8
  • SLC25A8BMIQ4
  • Solute carrier family 25 member 8
  • UCP 2
  • UCP2
  • UCPH
  • uncoupling protein 2 (mitochondrial, proton carrier)

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for UCP2 Antibody (NB100-59742)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-59742 Applications Species
Kim SY, Hong SW, Kim MO et al. S-adenosyl methionine prevents endothelial dysfunction by inducing heme oxygenase-1 in vascular endothelial cells. Mol Cells. 2013 Oct [PMID: 24046187] (WB, Human) WB Human
Vazquez-Medina JP, Popovich I, Thorwald MA et al. Angiotensin Receptor Mediated Oxidative Stress Is Associated with Impaired Cardiac Redox Signaling and Mitochondrial Function in Insulin Resistant Rats. Am J Physiol Heart Circ Physiol 2013 Jun 14 [PMID: 23771688] (WB, Rat) WB Rat
Montez P, Vazquez-Medina JP, Rodriguez R et al. Angiotensin receptor blockade recovers hepatic UCP2 expression and aconitase and SDH activities and ameliorates hepatic oxidative damage in insulin resistant rats Endocrinology. 2012 Dec [PMID: 23087176] (WB, Rat) WB Rat
De Souza CT, Araujo EP, Stoppiglia LF et al. Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action. FASEB J 2007 Jan 5 [PMID: 17209127]
Bhattacharyya SN, Chatterjee S, Chakrabarty Y Mitochondria Controlled mTORC1 Activation Compartmentalizes Translation Initiation Factor eIF4E to Augment Intracellular Trafficking and Extracellular Export of miRNA in Mammalian Cells bioRxiv Jan 1 2020 (WB, Human) WB Human
Pellegrin M, BouzourEne K, Aubert JF et al. Impact of aerobic exercise type on blood flow, muscle energy metabolism, and mitochondrial biogenesis in experimental lower extremity artery disease Sci Rep Aug 20 2020 [PMID: 32820213] (WB, Mouse) WB Mouse
Ghoshal B, Bertrand E, Bhattacharyya S Non-Canonical Ago Loading of EV-Derived Exogenous Single Stranded miRNA in Recipient Cells SSRN Journal Aug 27 2020 (ICC/IF, WB, Human, Mouse) ICC/IF, WB Human, Mouse

Reviews for UCP2 Antibody (NB100-59742) (2) 32

Average Rating: 3
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat.

Reviews using NB100-59742:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry UCP2 NB100-59742
Enlarge
2
reviewed by:
Anonymous
ICC Human 10/24/2020
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman primary fibroblast
SpeciesHuman
Lots6p2

Comments

CommentsFixation: PFA 4% 15´ RT. Blocking/permeabilization: 1h in 10% DS in PBST (0.1% Triton). UCP2 antibody incubation O/N 4ºC 1/100 dilution in 10% DS in PBST (0.1%Triton). Secondary antibody: Donkey anti-Goat Alexa Fluor 647 1:500 1h RT.
***Novus Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
  4
reviewed by:
Anonymous
WB Rat 12/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedLiver mitochondrial protein preparations
SpeciesRat

Blocking

Blocking DetailsSee PMID: 23087176

Details

Detection NotesSee PMID: 23087176

Comments

CommentsPublished in PMID: 23087176.Independent review from antibodyresource.com

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for UCP2 Antibody (NB100-59742). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I'd like to measure UCP2 levels in various mouse tissues by western blot. I was wondering which of your antibodies would be best for this purpose. In the literature I have seen some papers where they have isolated mitochondria to be able to see UCP2 and others where they have not. We would like to use frozen tissue samples, so isolating mitochondria will not be an option. We would like to use liver, muscle, pancreas, kidney, brown fat, subcutaneous fat, and gonadal fat. I'd appreciate any advice you might have.
    • Regarding your question on our UCP2 antibodies, we do have a product (catalog # NB100-78377), but our lab confirmed that it has only been validated in mouse liver and brain and there is no guarantee on other tissues.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

3
5
0
4
1
3
0
2
1
1
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Anonymous
10/24/2020
Application: ICC
Species: Human

 
Anonymous
12/19/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol UCP2
Entrez
Uniprot