mtTFA Antibody (18G102B2E11)

Images

 
Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Genetic Strategies: Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Biological Strategies: Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for ...read more
Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: ...read more
Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Total protein from Hela and U937 were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.
Biological Strategies: Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.
Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for 0-72 h before assessing mitochondrial biogenesis and respiration. (D) The level of TFAM ...read more
Dopaminergic neuron derivation from H9 stem cells and hIPSCs.Scheme showing the different steps of the DA neuron derivation protocol (A). Immunolabelings of cultured H9 (top panels) and WT hiPSCs (bottom panels) for ...read more
Patupilone counteracts hypoxia- but not IR-induced LOX-secretion. (A) Western blot of basal and IR-induced LOX in conditioned media derived from patupilone-untreated or patupilone-pretreated (0.5 nM) cells and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD, KO
Clone
18G102B2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

mtTFA Antibody (18G102B2E11) Summary

Immunogen
Human mtTFA [Swiss-Prot# Q00059]
Localization
Mitochondrion.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Knockout Validated reported in scientific literature (PMID 32001952)
  • Simple Western 1:20
  • Western Blot 1:1000
Application Notes
In Western blot, a band can be seen around 20 - 25 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
cIAP-2/HIAP-1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-71648 in the following applications:

Publications
Read Publications using
NBP1-71648 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28053692)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for mtTFA Antibody (18G102B2E11)

  • mitochondrial transcription factor A
  • MtTF1
  • mtTFA
  • TCF-6
  • TCF6L1
  • TCF6L2Mitochondrial transcription factor 1
  • TCF6TCF6L3
  • Transcription factor 6
  • Transcription factor 6-like 2 (mitochondrial transcription factor)
  • Transcription factor 6-like 2
  • transcription factor A, mitochondrial

Background

mtTFA (transcription factor A, mitochondrial) is an essential protein that binds mitochondrial DNA (mtDNA) with or without sequence specificity to control mitochondrial transcription initiation and mtDNA copy number. mtTFA exists as monomer localized in mitochondrion matrix/nucleoid where it binds DNA and interacts with TFB1M/TFB2M. mtTFA associates with mitochondrial light strand promoter for controlling transcription and is essential for correct/efficient mtRNA polymerase promoter recognition. mtTFA is capable of unwinding DNA and bending mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes, and mtTFA is essential for controlling mtDNA levels as well as its organization/compaction. mtDNA is a multicopy, circular genome that is required for respiratory function and interference with mitochondrial genome sequence fidelity, transcription, or translation can cause ATP insufficiency and several pathologies including progressive external ophthalmoplegia, amyotrophic lateral sclerosis (ALS), Alpers' disease, Parkinson's disease, Alzheimer's disease, aging, cardiovascular disease, diabetes and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mtTFA Antibody (NBP1-71648)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC-P, KO, Simple Western, WB.


Filter By Application
ELISA
(1)
ICC/IF
(4)
IHC-P
(1)
KO
(1)
Simple Western
(1)
WB
(6)
All Applications
Filter By Species
Human
(8)
Mouse
(3)
Rat
(2)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-71648 Applications Species
Gui J, Qiao W, Hu C et al. Chemotherapy potentiates CD8+ T cell cytotoxicity through stimulating cancer cell-autonomous type I IFN induction via oxidized mtDNA sensing Research Square 2022-12-06 (ICC/IF, Mouse) ICC/IF Mouse
Xu W, Zhao L. An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA-Protein Cross-Links from Mammalian Cells DNA (Basel) 2023-08-21 [PMID: 37601565] (ELISA) ELISA
Saal KA, Shaib AH, Mougios N et al. Heat denaturation enables multicolor X10-STED microscopy Scientific reports 2023-04-01 [PMID: 37005431] (ICC/IF, Human) ICC/IF Human
Xu W, Zhao L An Enzyme-Linked Immunosorbent Assay for the Detection of Mitochondrial DNA-Protein Cross-Links from Mammalian Cells DNA 2022-11-11 (WB, Human) WB Human
Xu R, Huang L, Liu J et al. Remodeling of Mitochondrial Metabolism by a Mitochondria-Targeted RNAi Nanoplatform for Effective Cancer Therapy Small (Weinheim an der Bergstrasse, Germany) 2023-11-02 [PMID: 37919865]
Ilamathi H, Benhammouda S, Lounas A et al. Contact sites between endoplasmic reticulum sheets and mitochondria regulate mitochondrial DNA replication and segregation iScience 2023-06-01 [PMID: 37534187] (ICC/IF, Human) ICC/IF Human
Vidali S, Feichtinger RG, Emberger M et al. Ageing is associated with a reduction in markers of mitochondrial energy metabolism in the human epidermis Experimental dermatology 2023-02-27 [PMID: 36851889] (IHC-P, Human) IHC-P Human
Limagne E, Nuttin L, Thibaudin M et al. MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells Cancer cell 2022-01-17 [PMID: 35051357]
Patrick S, Gowda P, Lathoria K Et al. Diminished YAP1 affects mitochondrial dynamics in IDH1 mutant glioma Journal of cell science 2021-10-15 [PMID: 34651186]
SA Harper, JR Bassler, S Peramsetty, Y Yang, LM Roberts, D Drummer, RT Mankowski, C Leeuwenbur, K Ricart, RP Patel, MM Bamman, SD Anton, BC Jaeger, TW Buford Resveratrol and exercise combined to treat functional limitations in late life: A pilot randomized controlled trial Exp Gerontol, 2020-10-15;0(0):111111. 2020-10-15 [PMID: 33068691] (Simple Western, Human) Simple Western Human
Show All 19 Publications.

Reviews for mtTFA Antibody (NBP1-71648) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-71648:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry mtTFA NBP1-71648
Enlarge
4
reviewed by:
Prashant Rai
ICC Mouse 08/27/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedmice primary macrophages
SpeciesMouse
LotA-8

Comments

Comments0.1 % Triton X100 permeabilization
Western Blot mtTFA NBP1-71648
Enlarge
4
reviewed by:
Verified Customer
WB Rat 03/07/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesRat
Lota1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mtTFA Antibody (NBP1-71648) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for mtTFA Antibody (NBP1-71648)

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Blogs on mtTFA

There are no specific blogs for mtTFA, but you can read our latest blog posts.
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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Prashant Rai
08/27/2021
Application: ICC
Species: Mouse

Verified Customer
03/07/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol TFAM
Entrez
Uniprot