mtTFA Antibody (18G102B2E11)

Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide (EtBr) for 8A weeks. Transfection with 30A nmol/L negative or TFAM siRNA for 8A days. TFAM protein relative toI2-actin. D, MtDNA levels. E, LPS-induced TNF-I+-and IL-8 release. Image collected and cropped by Cite Ab from the following publication (Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-g. J Allergy Clin Immunol (2017)) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: Mitotracker Red staining mitochondria. Image courtesy of Elizabeth Wang.

Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for 0AE

Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.

Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.

Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.

Immunocytochemistry/Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Antibody was tested in HeLa cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD, KO
• Clone: 18G102B2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

mtTFA Antibody (18G102B2E11) Summary

• Immunogen: Human mtTFA [Swiss-Prot# Q00059]
• Localization: Mitochondrion.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TFAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Knockout Validated
• Application Notes: This mtTFA (18G102B2E11) antibody is useful for ICC/IF, IHC on paraffin-embedded sections and Western blot, where a band can be seen around 20 - 25 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockout validation (PMID: 32001952).
• Control:

  cIAP-2/HIAP-1 Overexpression Lysate

Reactivity Notes

Human and rat. Mouse reactivity reported in scientific literature (PMID: 28053692)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for mtTFA Antibody (18G102B2E11)

• mitochondrial transcription factor A
• MtTF1
• mtTFA
• TCF-6
• TCF6L1
• TCF6L2Mitochondrial transcription factor 1
• TCF6TCF6L3
• Transcription factor 6
• Transcription factor 6-like 2 (mitochondrial transcription factor)
• Transcription factor 6-like 2
• transcription factor A, mitochondrial

Background

mtTFA (transcription factor A, mitochondrial) is an essential protein that binds mitochondrial DNA (mtDNA) with or without sequence specificity to control mitochondrial transcription initiation and mtDNA copy number. mtTFA exists as monomer localized in mitochondrion matrix/nucleoid where it binds DNA and interacts with TFB1M/TFB2M. mtTFA associates with mitochondrial light strand promoter for controlling transcription and is essential for correct/efficient mtRNA polymerase promoter recognition. mtTFA is capable of unwinding DNA and bending mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes, and mtTFA is essential for controlling mtDNA levels as well as its organization/compaction. mtDNA is a multicopy, circular genome that is required for respiratory function and interference with mitochondrial genome sequence fidelity, transcription, or translation can cause ATP insufficiency and several pathologies including progressive external ophthalmoplegia, amyotrophic lateral sclerosis (ALS), Alpers' disease, Parkinson's disease, Alzheimer's disease, aging, cardiovascular disease, diabetes and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for mtTFA Antibody (NBP1-71648)(9)

Publications using NBP1-71648

• Takishita Y, Yasuda H, Shimizu M et al. Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells J Clin Biochem Nutr Jan 1 2020 [PMID: 32001952] (WB, KO, Mouse)
• Widdrington JD, Gomez-Duran A, Pyle A et al. Exposure of Monocytic Cells to Lipopolysaccharide Induces Coordinated Endotoxin Tolerance, Mitochondrial Biogenesis, Mitophagy, and Antioxidant Defenses. Front Immunol. Sep 27 2018 [PMID: 30319656] (WB, Human)
• Dlaskova A, Engstova H, Spacek T et al. 3D super-resolution microscopy reflects mitochondrial cristae alternations and mtDNA nucleoid size and distribution Biochim Biochim Biophys Acta Bioenerg. 2018 May 1 [PMID: 29727614] (ICC/IF, Human)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.
• Huang C, Wang K, Yang Y et al. Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation Phytomedicine 2018 Jan 01 [PMID: 29433686] (Rat)
• Widdrington JD, Gomez-Duran A, Steyn JS et al. Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-gamma J. Allergy Clin. Immunol. 2017 Jun 17 [PMID: 28629747] (WB, Human)
• Mastrocola R, Penna C et al. Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways. Oxid Med Cell Longev Jun 1 2017 [PMID: 28053692] (WB, Mouse)
• Sun Q, Zhong W, Zhang W, Zhou Z Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency. Am J Physiol Gastrointest Liver Physiol 2016 Feb 1 [PMID: 26585415]
• Choi J, Chandrasekaran K, Demarest TG et al. Brain diabetic neurodegeneration segregates with low intrinsic aerobic capacity. Annals of Clinical and Translational Neurology 2014 [PMID: 25356430]
• Morris EM, Meers GM, Booth FW et al. PGC-1alpha Overexpression Results in Increased Hepatic Fatty Acid Oxidation with Reduced Triacylglycerol Accumulation and Secretion Am J Physiol Gastrointest Liver Physiol 2012 Aug 16 [PMID: 22899824] (WB, Rat)

Review for mtTFA Antibody (NBP1-71648) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous WB Rat 03/07/2018

Summary

• Application: Western Blot
• Sample Tested: Liver
• Species: Rat
• Lot: a1

Bioinformatics

• Gene Symbol: TFAM
• Entrez:
  • 7019(Human)
• Uniprot:
  • Q00059(Human)