mtTFA Antibody (18G102B2E11)

Images

 
Simple Western: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Simple Western lane view shows a specific band for mtTFA in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 ...read more
Genetic Strategies: Knockdown Validated: mtTFA Antibody (18G102B2E11) [NBP1-71648] - MtDNA depletion and reversible impaired immune functions in THP-1A cells. A and B, Treatment with 50A ng/mL ethidium bromide ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Biological Strategies: Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Induction of mitochondrial biogenesis following exposure of THP-1 cells to LPS. THP-1 cells were incubated with LPS (100 ng/ml) for ...read more
Immunohistochemistry-Paraffin: mtTFA Antibody (18G102B2E11) [NBP1-71648] - IHC staining of mtTFA on human testis using DAB with hematoxylin counterstain.
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells ...read more
Immunocytochemistry/ Immunofluorescence: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Immunostaining in HeLa cells. C1: DAPI staining 2 nuclei, C2: anti-mtTFA stain with Alexa Fluor 488 secondary ab (green), C3: ...read more
Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Total protein from Hela and U937 were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was ...read more
Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Analysis of mtTFA expression in HeLa whole cell lysate.
Biological Strategies: Western Blot: mtTFA Antibody (18G102B2E11) [NBP1-71648] - Alcohol feeding significantly decreased TFAM expression in rat liver. This image was submitted by a customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, KD, KO
Clone
18G102B2E11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

mtTFA Antibody (18G102B2E11) Summary

Immunogen
Human mtTFA [Swiss-Prot# Q00059]
Localization
Mitochondrion.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFAM
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Knockout Validated
  • Simple Western 1:20
  • Western Blot 1:1000
Application Notes
This mtTFA (18G102B2E11) antibody is useful for ICC/IF, IHC on paraffin-embedded sections and Western blot, where a band can be seen around 20 - 25 kDa. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Knockout validation (PMID: 32001952).
Control
cIAP-2/HIAP-1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 4
using
NBP1-71648 in the following applications:

Publications
Read Publications using
NBP1-71648 in the following applications:

  • 1 publication
  • KO
    1 publication
  • WB
    5 publications

Reactivity Notes

Human and rat. Mouse reactivity reported in scientific literature (PMID: 28053692)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for mtTFA Antibody (18G102B2E11)

  • mitochondrial transcription factor A
  • MtTF1
  • mtTFA
  • TCF-6
  • TCF6L1
  • TCF6L2Mitochondrial transcription factor 1
  • TCF6TCF6L3
  • Transcription factor 6
  • Transcription factor 6-like 2 (mitochondrial transcription factor)
  • Transcription factor 6-like 2
  • transcription factor A, mitochondrial

Background

mtTFA (transcription factor A, mitochondrial) is an essential protein that binds mitochondrial DNA (mtDNA) with or without sequence specificity to control mitochondrial transcription initiation and mtDNA copy number. mtTFA exists as monomer localized in mitochondrion matrix/nucleoid where it binds DNA and interacts with TFB1M/TFB2M. mtTFA associates with mitochondrial light strand promoter for controlling transcription and is essential for correct/efficient mtRNA polymerase promoter recognition. mtTFA is capable of unwinding DNA and bending mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes, and mtTFA is essential for controlling mtDNA levels as well as its organization/compaction. mtDNA is a multicopy, circular genome that is required for respiratory function and interference with mitochondrial genome sequence fidelity, transcription, or translation can cause ATP insufficiency and several pathologies including progressive external ophthalmoplegia, amyotrophic lateral sclerosis (ALS), Alpers' disease, Parkinson's disease, Alzheimer's disease, aging, cardiovascular disease, diabetes and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-71648
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD, KO

Publications for mtTFA Antibody (NBP1-71648)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, KO, WB.


Filter By Application
ICC/IF
(1)
KO
(1)
WB
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 10.
Publications using NBP1-71648 Applications Species
Patrick S, Gowda P, Lathoria K Et al. Diminished YAP1 affects mitochondrial dynamics in IDH1 mutant glioma Journal of cell science Oct 15 2021 [PMID: 34651186]
Takishita Y, Yasuda H, Shimizu M et al. Formation of neutrophil extracellular traps in mitochondrial DNA-deficient cells J Clin Biochem Nutr Jan 1 2020 [PMID: 32001952] (WB, KO, Mouse) WB, KO Mouse
Widdrington JD, Gomez-Duran A, Pyle A et al. Exposure of Monocytic Cells to Lipopolysaccharide Induces Coordinated Endotoxin Tolerance, Mitochondrial Biogenesis, Mitophagy, and Antioxidant Defenses. Front Immunol. Sep 27 2018 [PMID: 30319656] (WB, Human) WB Human
Dlaskova A, Engstova H, Spacek T et al. 3D super-resolution microscopy reflects mitochondrial cristae alternations and mtDNA nucleoid size and distribution Biochim Biochim Biophys Acta Bioenerg. 2018 May 1 [PMID: 29727614] (ICC/IF, Human)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
ICC/IF Human
Huang C, Wang K, Yang Y et al. Gastrodia elata alleviates mutant huntingtin aggregation through mitochondrial function and biogenesis mediation Phytomedicine 2018 Jan 01 [PMID: 29433686] (Rat) Rat
Widdrington JD, Gomez-Duran A, Steyn JS et al. Mitochondrial DNA depletion induces innate immune dysfunction rescued by IFN-gamma J. Allergy Clin. Immunol. 2017 Jun 17 [PMID: 28629747] (WB, Human) WB Human
Mastrocola R, Penna C et al. Pharmacological Inhibition of NLRP3 Inflammasome Attenuates Myocardial Ischemia/Reperfusion Injury by Activation of RISK and Mitochondrial Pathways. Oxid Med Cell Longev Jun 1 2017 [PMID: 28053692] (WB, Mouse) WB Mouse
Sun Q, Zhong W, Zhang W, Zhou Z Defect of mitochondrial respiratory chain is a mechanism of ROS overproduction in a rat model of alcoholic liver disease: role of zinc deficiency. Am J Physiol Gastrointest Liver Physiol 2016 Feb 1 [PMID: 26585415]
Choi J, Chandrasekaran K, Demarest TG et al. Brain diabetic neurodegeneration segregates with low intrinsic aerobic capacity. Annals of Clinical and Translational Neurology 2014 [PMID: 25356430]
Morris EM, Meers GM, Booth FW et al. PGC-1alpha Overexpression Results in Increased Hepatic Fatty Acid Oxidation with Reduced Triacylglycerol Accumulation and Secretion Am J Physiol Gastrointest Liver Physiol 2012 Aug 16 [PMID: 22899824] (WB, Rat) WB Rat

Reviews for mtTFA Antibody (NBP1-71648) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NBP1-71648:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry mtTFA NBP1-71648
Enlarge
4
reviewed by:
Prashant Rai
ICC Mouse 08/27/2021
View

Summary

ApplicationImmunocytochemistry
Sample Testedmice primary macrophages
SpeciesMouse
LotA-8

Comments

Comments0.1 % Triton X100 permeabilization
Western Blot mtTFA NBP1-71648
Enlarge
4
reviewed by:
Verified Customer
WB Rat 03/07/2018
View

Summary

ApplicationWestern Blot
Sample TestedLiver
SpeciesRat
Lota1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for mtTFA Antibody (NBP1-71648) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-71648AF350
Alexa Fluor 405 NBP1-71648AF405
Alexa Fluor 488 NBP1-71648AF488
Alexa Fluor 532 NBP1-71648AF532
Alexa Fluor 594 NBP1-71648AF594
Alexa Fluor 647 NBP1-71648AF647
Alexa Fluor 700 NBP1-71648AF700
Alexa Fluor 750 NBP1-71648AF750
Biotin NBP1-71648B
DyLight 350 NBP1-71648UV
DyLight 405 NBP1-71648V
DyLight 488 NBP1-71648G
DyLight 550 NBP1-71648R
DyLight 594 NBP1-71648DL594
DyLight 650 NBP1-71648C
DyLight 680 NBP1-71648FR
DyLight 755 NBP1-71648IR
FITC NBP1-71648F
HRP NBP1-71648H
Janelia Fluor 549 NBP1-71648JF549
Janelia Fluor 646 NBP1-71648JF646

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Prashant Rai
08/27/2021
Application: ICC
Species: Mouse

Verified Customer
03/07/2018
Application: WB
Species: Rat

Bioinformatics

Gene Symbol TFAM
Entrez
Uniprot