Glut4 Antibody

Images

 
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk ...read more
Immunocytochemistry/ Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with Glut4 Antibody NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with ...read more
Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A. MCF7 whole cell lysate and B. 3T3L1 whole cell lysate
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.
Biological Strategies: Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Glut4 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509) [UniProt P14672]
Localization
Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin
  • Flow (Intracellular)
Application Notes
This GLUT4 antibody is useful for Western blot, IHC and ICC/IF. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24339864). Flow cytometry data from customer review.
Positive Control
MCF-7 Lysate (NB820-59465)
Control Peptide
Glut4 Peptide (NBP1-49533PEP)
Reviewed Applications
Read 3 Reviews rated 4
using
NBP1-49533 in the following applications:

Publications
Read Publications using
NBP1-49533 in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with rat based on 100% sequence homology. Rat reactivity reported in scientific literature (PMID: 26468734)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut4 Antibody

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glucose Transporter GLUT4 (SLC2A4 or GLUT4) is an insulin-sensitive glucose transporter which facilitate insulin-stimulated glucose uptake in adipose tissue, skeletal muscle and cardiac tissues (that specifically express GLUT4). GLUT4 is a 12-transmembrane domain/ multi-pass membrane protein localized in endomembrane system as well as cytoplasm near perinuclear region, and facilitates glucose transport in the direction of glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation via TBC1D4 phosphorylation. Transgenic mice lacking or overexpressing GLUT4 respectively decrease or increase whole-body insulin sensitivity, suggesting its role in maintenance of glucose homeostasis, and accordingly, under obesity conditions, reduction of GLUT4 gene expression is directly related to insulin resistance development. Inflammatory cytokines produced by the adipose tissue including TNF-alpha, IL-6 etc have been related to reduce GLUT4 expression and defective GLUT4 is an underlying cause of NIDDM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP1-49533)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
WB
(6)
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Filter By Species
Human
(1)
Mouse
(4)
Rat
(2)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-49533 Applications Species
Munoz VR, Gaspar RC, Kuga GK et al. Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle. J. Cell. Physiol. 2017 Dec 08 [PMID: 29219181] (WB, Rat) WB Rat
Marinozzi MR, fi LP, Malatesta M et al. Innovative approach to safely induce controlled lipolysis by superparamagnetic iron oxide nanoparticles-mediated hyperthermic treatment. Int J Biochem Cell Biol. 2017 Oct 27 [PMID: 29111382] (Mouse) Mouse
Kang HW, Lim WC, Lee JK et al. Germinated Waxy Black Rice Ameliorates Hyperglycemia and Dyslipidemia in Streptozotocin-Induced Diabetic Rats Biol. Pharm. Bull. 2017 [PMID: 29093331] (WB) WB
Molina Sa, Moriarty HK, Infield DT et al. Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner. Am. J. Physiol. Lung Cell Mol. Physiol. May 1 2017 [PMID: 28213469] (WB, ICC/IF, IHC, Human) WB, ICC/IF, IHC Human
Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ. Glucagon-like peptide 1 (GLP-1)-based therapy upregulate LXR-ABCA1/ABCG1 cascade in adipocytes. Biochem. Biophys. Res. Commun. 2015 Nov 18 [PMID: 26603933] (WB, Mouse) WB Mouse
Kain V, Kapadia B, Viswakarma N et al. Co-activator binding protein PIMT mediates TNF-a induced insulin resistance in skeletal muscle via the transcriptional down-regulation of MEF2A and GLUT4. Sci Rep. 2015 Oct 16 [PMID: 26468734] (WB, Rat) WB Rat
Zidek LM, Ackermann T, Hartleben G et al. Deficiency in mTORC1-controlled C/EBPbeta-mRNA translation improves metabolic health in mice. EMBO Rep 2015 Aug [PMID: 26113365] (WB, Mouse) WB Mouse
Gauger KJ, Bassa LM, Henchey EM et al. Mice Deficient in Sfrp1 Exhibit Increased Adiposity, Dysregulated Glucose Metabolism, and Enhanced Macrophage Infiltration. PLoS One 2013 Dec 5 [PMID: 24339864] (IHC-P, Mouse) IHC-P Mouse

Reviews for Glut4 Antibody (NBP1-49533) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-49533:
Filter by Applications
WB
(3)
All Applications
Filter by Species
Human
(1)
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut4 NBP1-49533
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reviewed by:
Anonymous
WB Mouse 03/27/2018
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Summary

ApplicationWestern Blot
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse
LotE-1

Comments

CommentsWestern Blot: Glut4 Antibody [NBP1-49533SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk.
Western Blot Glut4 NBP1-49533
Enlarge
reviewed by:
Frances Bell
WB Mouse 03/28/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotD1

Comments

CommentsC57BL/6 Mice
Western Blot Glut4 NBP1-49533
Enlarge
reviewed by:
Marie-estelle LOSFELD
WB Human 03/09/2014
View

Summary

ApplicationWestern Blot
Sample Testedlung cancer cells
SpeciesHuman

Blocking

Blocking Details5% milk in TBS tween

Primary Anitbody

Dilution Ratio1/1000 incubation 3h RT in 5% milk in TBS-tween

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut4 Antibody (NBP1-49533). (Showing 1 - 5 of 5 FAQs).

  1. NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as internal means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?
    • We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as internal region.
  2. Do you happen to sell peptide for this antibody?
    • We do have a peptide for this particular product. The catalog number is NBP1-49533PEP.
  3. Would you please help confirm if NBP1-49533 has been tested for IHC with frozen sections?
    • NBP1-49533 has been validated in WB, ICC/IF and IHC-P, but has not been validated in IHC-Fr yet. However, because this antibody worked very well in ICC/IF, I strongly believe that it will work in IHC-Fr also. Please see the protocol for IHC-Fr which is utilized in our lab.
  4. NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as "internal" means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?
    • We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as "internal" region.
  5. Regarding NBP1-49533AF647, Glut4-Alexa Fluor 647, the data sheet declares that the immunogen is aa480-509. Uniport says it’s a cytoplasmatic region of the protein. Is this antibody suitable for flow? Has it been validated for this application? Can you provide the suggested protocol, specifically the fixation and permeabilzation part? Any available references for this antibody and application?
    • This antibody was validated in flow cytometry. We used our intracellular flow protocol found here: http://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html. The results from this testing can be seen on the unconjugated part datasheet here: http://www.novusbio.com/Glut4-Antibody_NBP1-49533.html.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49533AF405
Alexa Fluor 488 Labeled NBP1-49533AF488
Alexa Fluor 647 Labeled NBP1-49533AF647
Alexa Fluor 700 Labeled NBP1-49533AF700
Biotin Labeled NBP1-49533B
DyLight 405 Labeled NBP1-49533V
DyLight 488 Labeled NBP1-49533G
DyLight 550 Labeled NBP1-49533R
DyLight 650 Labeled NBP1-49533C
FITC Labeled NBP1-49533F
HRP Labeled NBP1-49533H

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Blogs on Glut4.

Glucose Transporter 4 (GLUT4, SLC2A4)
GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

Anonymous
03/27/2018
Application: WB
Species: Mouse

Frances Bell
03/28/2017
Application: WB
Species: Mouse

Marie-estelle LOSFELD
03/09/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol SLC2A4
Entrez
Uniprot