Glut4 Antibody - BSA Free

Images

 
Western Blot: Glut4 Antibody [NBP1-49533] - Glycolysis-related genes and proteins were upregulated in differentiated podocytes. Representative blot images of glycolysis-related proteins (n =3). d All proteins were ...read more
Immunocytochemistry/ Immunofluorescence: Glut4 Antibody [NBP1-49533] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were ...read more
Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney
Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. ...read more
Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in ...read more
Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk ...read more
Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.
Biological Strategies: Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PlSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Glut4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide from the human Glucose Transporter GLUT4 protein [UniProt P14672]
Localization
Endomembrane system; Multi-pass membrane protein. Cytoplasm - perinuclear region. Note: Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC2A4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 0.5ug/ml
Application Notes
This GLUT4 antibody is useful for Western blot, IHC and ICC/IF. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 24339864). Flow cytometry data from customer review. Knockdown validation (PMID: 31555030).
Control
MCF-7 Whole Cell Lysate
Control Peptide
Glut4 Peptide (NBP1-49533PEP)
Reviewed Applications
Read 4 Reviews rated 4
using
NBP1-49533 in the following applications:

Publications
Read Publications using
NBP1-49533 in the following applications:

  • 3 publications
  • 5 publications
  • IHC
    2 publications
  • 2 publications
  • KD
    1 publication
  • 1 publication
  • WB
    11 publications

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 31555030).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glut4 Antibody - BSA Free

  • Glut4
  • GLUT-4
  • GLUT4Glucose transporter type 4, insulin-responsive
  • insulin-responsive glucose transporter type 4
  • SLC2A4
  • solute carrier family 2 (facilitated glucose transporter), member 4
  • solute carrier family 2, facilitated glucose transporter member 4

Background

Glucose Transporter GLUT4 (SLC2A4 or GLUT4) is an insulin-sensitive glucose transporter which facilitate insulin-stimulated glucose uptake in adipose tissue, skeletal muscle and cardiac tissues (that specifically express GLUT4). GLUT4 is a 12-transmembrane domain/ multi-pass membrane protein localized in endomembrane system as well as cytoplasm near perinuclear region, and facilitates glucose transport in the direction of glucose gradient. This transporter localizes to intracellular organelles (endosomes) in unstimulated cells and translocates to the cell surface following insulin stimulation via TBC1D4 phosphorylation. Transgenic mice lacking or overexpressing GLUT4 respectively decrease or increase whole-body insulin sensitivity, suggesting its role in maintenance of glucose homeostasis, and accordingly, under obesity conditions, reduction of GLUT4 gene expression is directly related to insulin resistance development. Inflammatory cytokines produced by the adipose tissue including TNF-alpha, IL-6 etc have been related to reduce GLUT4 expression and defective GLUT4 is an underlying cause of NIDDM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glut4 Antibody (NBP1-49533)(25)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Plant.

We have publications tested in 7 applications: Flow, ICC/IF, IHC, IHC-P, KD, PAGE, WB.


Filter By Application
Flow
(3)
ICC/IF
(5)
IHC
(2)
IHC-P
(2)
KD
(1)
PAGE
(1)
WB
(11)
All Applications
Filter By Species
Human
(5)
Mouse
(10)
Rat
(5)
Plant
(2)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NBP1-49533 Applications Species
Wei S, Li C, Luo X et al. Intermittent protein restriction protects islet beta cells and improves glucose homeostasis in diabetic mice Science Bulletin Dec 1 2021 (ICC/IF, Mouse) ICC/IF Mouse
Melo BP, Zacarias AC, Oliveira JCC Et al. Combination of Aerobic Training and Cocoa Flavanols as Effective Therapies to Reduce Metabolic and Inflammatory Disruptions in Insulin-Resistant Rats: The Exercise, Cocoa, and Diabetes Study International journal of sport nutrition and exercise metabolism Nov 22 2021 [PMID: 34808598]
Zeng Z, Nian Q, Chen N Et al. Ginsenoside Rg3 inhibits angiogenesis in gastric precancerous lesions through downregulation of Glut1 and Glut4 Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie Oct 11 2021 [PMID: 34799220] (IHC-P, Human) IHC-P Human
Kou YB, Yan XQ, Jing QY Et al. LIGHT (TNFSF14) inhibits glucose uptake of adipocytes by downregulating GLUT4 expression via AKT signaling pathway Biochemical and biophysical research communications Oct 28 2021 [PMID: 34735871] (ICC/IF, WB) ICC/IF, WB
Haba D, Nakagami G, Minematsu T, Sanada H Low-frequency vibration promotes AMPK-mediated glucose uptake in 3T3-L1 adipocytes Heliyon Sep 1 2021 [PMID: 34568592] (Human) Human
Yuan Q, Miao J, Yang Q et al. Role of pyruvate kinase M2-mediated metabolic reprogramming during podocyte differentiation Cell Death Dis May 11 2020 [PMID: 32393782] (WB, Mouse) WB Mouse
De Carvalho F, Munoz V, Brandao C Et Al. Taurine upregulates insulin signaling and mitochondrial metabolism in vitro, but not in adipocytes of obese women Nutrition Jul 1 2021
Wei S, Li C, Luo X et al. Intermittent protein restriction improves glucose homeostasis and restores damaged islet beta cells in diabetic mice Research Square Dec 10 2020 (ICC/IF, Mouse) ICC/IF Mouse
Wani IA, Kumar V, Verma S et al. Dactylorhiza hatagirea (D. Don) Soo: A Critically Endangered Perennial Orchid from the North-West Himalayas Plants (Basel) Nov 25 2020 [PMID: 33255646]
Reijrink M, de Boer SA, Antunes IF et al. [18F]FDG Uptake in Adipose Tissue Is Not Related to Inflammation in Type 2 Diabetes Mellitus Mol Imaging Biol Sep 4 2020 [PMID: 32886301] (IHC, Human) IHC Human
Show All 25 Publications.

Reviews for Glut4 Antibody (NBP1-49533) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 2 species: Human, Mouse.

Reviews using NBP1-49533:
Filter by Applications
Western Blot
(4)
All Applications
Filter by Species
Human
(1)
Mouse
(3)
All Species
Images Ratings Applications Species Date Details
Western Blot Glut4 NBP1-49533
Enlarge
4
reviewed by:
Jaroslawna Meister
Western Blot Mouse 04/23/2019
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotF-3
Western Blot Glut4 NBP1-49533
Enlarge
3
reviewed by:
Francesca Pacifici
Western Blot Mouse 03/27/2018
View

Summary

ApplicationWestern Blot
Sample Tested3T3-L1 mouse embryonic fibroblast adipose-like cell line
SpeciesMouse
LotE-1

Comments

CommentsWestern Blot: Glut4 Antibody [NBP1-49533SS] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated on a 4-12% gel by SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk.
Western Blot Glut4 NBP1-49533
Enlarge
5
reviewed by:
Verified Customer
Western Blot Mouse 03/28/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse skeletal muscle
SpeciesMouse
LotD1

Comments

CommentsC57BL/6 Mice
Western Blot Glut4 NBP1-49533
Enlarge
4
reviewed by:
Verified Customer
Western Blot Human 03/09/2014
View

Summary

ApplicationWestern Blot
Sample Testedlung cancer cells
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glut4 Antibody (NBP1-49533). (Showing 1 - 6 of 6 FAQs).

  1. NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as internal means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?
    • We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as internal region.
  2. Do you happen to sell peptide for this antibody?
    • We do have a peptide for this particular product. The catalog number is NBP1-49533PEP.
  3. Would you please help confirm if NBP1-49533 has been tested for IHC with frozen sections?
    • NBP1-49533 has been validated in WB, ICC/IF and IHC-P, but has not been validated in IHC-Fr yet. However, because this antibody worked very well in ICC/IF, I strongly believe that it will work in IHC-Fr also. Please see the protocol for IHC-Fr which is utilized in our lab.
  4. NBP1-49533 and NBP1-49533R are listed as being raised against a synthetic peptide made to an internal portion of the human GLUT4 protein (between residues 480-509 or 480-530 for NBP1-49533R), but since the protein is only 509aa in length, I am curious to know if the description as "internal" means that the actual sequence does not include the very c-terminal part, or if the listed sequence range is incorrect and the actual sequence in more internal?
    • We highly appreciate your feedback on the immunogen info on the datasheet of our Glucose Transporter GLUT4 antibody #NBP1-49533, and yes, the immunogen sequence is from human Glucose Transporter GLUT4 protein between residues 480-509. Similarly, the sequence for Glucose Transporter GLUT4 Antibody [DyLight 550] (NBP1-49533R) would also be the same (between residues 480-509). It is certainly C-terminal region and we apologize for the mistake committed on our end about writing it as "internal" region.
  5. Regarding NBP1-49533AF647, Glut4-Alexa Fluor 647, the data sheet declares that the immunogen is aa480-509. Uniport says it’s a cytoplasmatic region of the protein. Is this antibody suitable for flow? Has it been validated for this application? Can you provide the suggested protocol, specifically the fixation and permeabilzation part? Any available references for this antibody and application?
    • This antibody was validated in flow cytometry. We used our intracellular flow protocol found here: http://www.novusbio.com/support/support-by-application/flow-cytometry/protocol.html. The results from this testing can be seen on the unconjugated part datasheet here: http://www.novusbio.com/Glut4-Antibody_NBP1-49533.html.
  6. Regarding the use of NBP1-49533, is this anti Glut4 Antibody validated for extracellular domain of Glut4 in for flow cytometry? The data sheet shows images if intracellular and additional images of flow images that are not specified. 
    • NBP1-49533 is not specific for the extracellular domain of Glut4 becuase the immunogen of the antibody pertains to the cytoplasmic C-terminus of Glut4: Immunogen: A synthetic peptide made to a C-terminal portion of the human Glucose Transporter GLUT4 protein (between residues 480-509) [UniProt P14672]. Amino acids 480-509 correspond to the cytoplasmic tail of this protein, accordingly, NBP1-49533 has only been used for labeling the intracellular staining in FLOW cytometry.The only other option we have is MAB86541, which is made by our sister company R&D systems. They used their protocol for "Staining Membrane-associated Proteins" to product the Flow results, so if you have any other questions abotu this product I would contact them for the most accurate and proper information: https://www.rndsystems.com/resources/protocols/flow-cytometry-protocol-staining-membrane-associated-proteins-suspended-cells

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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GLUT4 is an insulin-sensitive glucose transporter that facilitates insulin-stimulated glucose uptake in adipose tissue, skeletal muscle, and cardiac tissues that specifically express this protein. It is a twelve transmembrane domain multi-pass protein...  Read full blog post.

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Recent Reviews

4
5
1
4
2
3
1
2
0
1
0

Jaroslawna Meister
04/23/2019
Application: Western Blot
Species: Mouse

Francesca Pacifici
03/27/2018
Application: Western Blot
Species: Mouse

Verified Customer
03/28/2017
Application: Western Blot
Species: Mouse

Bioinformatics

Gene Symbol SLC2A4
Entrez
Uniprot