ERR alpha/NR3B1 Antibody

Images

 
Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates
Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha ...read more
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

ERR alpha/NR3B1 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of human Estrogen Related Receptor alpha (within residues 1-50). [Swiss-Prot# P11474]
Localization
Nucleus, Cytoplasm
Predicted Species
Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ESRRA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
Application Notes
This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Raw 264.7 Lysate (NBP1-30157)
Control Peptide
ERR alpha/NR3B1 Peptide (NBP1-47254PEP)
Publications
Read Publications using
NBP1-47254 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human and mouse. Predicted to react with rat, pig and dog based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERR alpha/NR3B1 Antibody

  • ERR alpha
  • ERR1
  • ERR1Estrogen receptor-like 1
  • ERRa
  • ESRL1
  • ESRL1ERR-alpha
  • ESRRA
  • estrogen-related receptor alphaERRalpha
  • NR3B1
  • NR3B1Nuclear receptor subfamily 3 group B member 1
  • steroid hormone receptor ERR1

Background

Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

PP-H6812-00
Species: Hu
Applications: WB, IHC, IP, DirELISA
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-04676
Species: Hu, Mu, Rt, Ha, Sq
Applications: WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-90066
Species: Hu
Applications: WB, IHC, IHC-P
NB100-74413
Species: Hu, Mu, Rb
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB300-537
Species: Hu, Mu, Rt, Bv, Rb
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB300-748
Species: Hu, Rt
Applications: WB, ICC/IF
NBP1-90813
Species: Hu
Applications: WB, IHC, IHC-P
H00000034-D01P
Species: Hu, Mu, Rt
Applications: WB
NBP2-13674
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PP-N1665-00
Species: Hu
Applications: WB, IHC, IP
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-19586
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-1596
Species: Hu, Pm, Rb
Applications: WB, ICC/IF, IHC
AF4096
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB200-310
Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, IP, IF

Publications for ERR alpha/NR3B1 Antibody (NBP1-47254)(4)

Reviews for ERR alpha/NR3B1 Antibody (NBP1-47254) (0)

There are no reviews for ERR alpha/NR3B1 Antibody (NBP1-47254). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for ERR alpha/NR3B1 Antibody (NBP1-47254): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERR alpha/NR3B1 Antibody (NBP1-47254) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-47254AF405
Alexa Fluor 488 Labeled NBP1-47254AF488
Alexa Fluor 647 Labeled NBP1-47254AF647
Alexa Fluor 700 Labeled NBP1-47254AF700
Biotin Labeled NBP1-47254B
DyLight 405 Labeled NBP1-47254V
DyLight 488 Labeled NBP1-47254G
DyLight 550 Labeled NBP1-47254R
DyLight 650 Labeled NBP1-47254C
FITC Labeled NBP1-47254F
HRP Labeled NBP1-47254H

Additional ERR alpha/NR3B1 Products

Bioinformatics Tool for ERR alpha/NR3B1 Antibody (NBP1-47254)

Discover related pathways, diseases and genes to ERR alpha/NR3B1 Antibody (NBP1-47254). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ERR alpha/NR3B1 Antibody (NBP1-47254)

Discover more about diseases related to ERR alpha/NR3B1 Antibody (NBP1-47254).
 

Pathways for ERR alpha/NR3B1 Antibody (NBP1-47254)

View related products by pathway.

PTMs for ERR alpha/NR3B1 Antibody (NBP1-47254)

Learn more about PTMs related to ERR alpha/NR3B1 Antibody (NBP1-47254).
 

Research Areas for ERR alpha/NR3B1 Antibody (NBP1-47254)

Find related products by research area.

Blogs on ERR alpha/NR3B1

There are no specific blogs for ERR alpha/NR3B1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ERR alpha/NR3B1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ESRRA
Entrez
Uniprot