Western Blot: ERR alpha/NR3B1 Antibody [NBP1-47254] - Analysis of ERR alpha in A431 cell lysates
Immunocytochemistry/ Immunofluorescence: ERR alpha/NR3B1 Antibody [NBP1-47254] - ERR alpha antibody was tested in HeLa cells at a 1:100 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained ...read more
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha/ESRRA protein in a section of normal human kidney using 5 ug/ml concentration of Estrogen Related Receptor alpha ...read more
Immunohistochemistry-Paraffin: ERR alpha/NR3B1 Antibody [NBP1-47254] - IHC-P detection of ERR alpha / ESRRA protein in a section of normal skin of human using 5 ug/ml concentration of Estrogen Related Receptor alpha ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
Immunogen affinity purified
Western Blot 2 ug/ml
Immunohistochemistry 2 ug/ml
Immunohistochemistry-Paraffin 2 ug/ml
This Estrogen Related Receptor alpha antibody is useful for Western Blot, Immunohistochemistry-Paraffin and Immunocytochemistry/Immunofluorescence applications. ERR alpha/ ESRRA localize mainly to the nucleus, but there are reports on its cytoplasmic presence also, especially in cells with high nuclear expression. In a feedback loop mechanism, it has been shown that ERK8 /MAPK15 interacts with ERR alpha to induce its CRM1-dependent translocation to cytoplasm for inhibiting its transcriptional activity via regulation of its cellular localization (PMIDs 21190936, 18070081). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human and mouse. Predicted to react with rat, pig and dog based on 100% sequence homology.
Alternate Names for ERR alpha/NR3B1 Antibody
ERR1Estrogen receptor-like 1
estrogen-related receptor alphaERRalpha
NR3B1Nuclear receptor subfamily 3 group B member 1
steroid hormone receptor ERR1
Nuclear receptors are essential in embryonic development as well as for the regulation of adult physiological functions, and estrogen related receptor alpha (ERR-alpha) is one such receptors expressed in several tissues requiring high energy demand, including heart, skeletal muscle and brain. ERR-alpha binds to ERRE (ERR-alpha response element) containing a single consensus half-site, 5'-TNAAGGTCA-3'; to MCAD response element NRRE-1 for regulating MCAD promoter, and it also regulates lactoferrin gene promoter. ERR-alpha is an orphan nuclear hormone receptor capable of regulating transcription of genes involved in multiple cellular/physiological processes such as regulation of energy metabolism wherein it is co-activated by PPAR gamma and PGC-1 alpha to regulate several genes required for mitochondrial function. ERR-alpha regulates estrogen signaling and fatty acid oxidation/adaptative bioenergetic response, and is involved in cartilage/bone formation, macrophage activation and carcinogenesis. High ERR-alpha expression levels coupled with poor prognosis have been observed in various malignancies including colon, endometrium, ovary, prostate and breast cancers, and adrenocortical carcinoma.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.