PGC1 alpha Antibody [HRP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ha, Sq, Ca, Eq, PmSpecies Glossary
Applications WB, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

PGC1 alpha Antibody [HRP] Summary

Immunogen
Synthetic peptide made to an internal portion of the human PGC-1 alpha protein (within residues 400-550). [Swiss-Prot# Q9UBK2].
Localization
Nucleus when activated, can be cytoplasmic when inactive (PMID: PMC2253697).
Predicted Species
Equine (94%), Canine (93%), Primate (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARGC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This PGC-1 alpha antibody is useful for Western Blot and Immunohistochemistry on paraffin embedded sections. In WB, a band is seen at ~91 kDa representing PGC-1 alpha. In IHC-P, staining is very strong in the nucleus with some cytoplasmic staining. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24508229)

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Expected reactivity based on sequence identity: monkey/non-human primates (98%), equine (94%), and canine (93%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PGC1 alpha Antibody [HRP]

  • LEM6
  • Ligand effect modulator 6
  • ligand effect modulator-6
  • peroxisome proliferative activated receptor, gamma, coactivator 1, alpha
  • peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
  • PGC1 alpha
  • PGC1A
  • PGC-1-alpha
  • PGC1APGC-1(alpha)
  • PGC1peroxisome proliferative activated receptor, gamma, coactivator 1
  • PPAR gamma coactivator variant form
  • PPAR gamma coactivator-1
  • PPAR-gamma coactivator 1-alpha
  • PPARGC1A
  • PPARGC-1-alpha
  • PPARGC1PGC-1v

Background

PGC1 alpha is a transcriptional coactivator that regulates the genes involved in energy metabolism. It interacts with PPARgamma, which permits it's interaction with multiple transcription factors. PGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. It may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PGC1 alpha Antibody (NBP1-04676H). (Showing 1 - 2 of 2 FAQ).

  1. Please differentiate me between PPAR and PGC clearly.i am confuse to know the differ these two. waiting for your reply
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. What is isotype of this product?
    • It is a Rabbit polyclonal antibody and its isotype is IgG.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NBP1-04676B
DyLight 488 Labeled NBP1-04676G
DyLight 550 Labeled NBP1-04676R
DyLight 650 Labeled NBP1-04676C

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Bioinformatics

Gene Symbol PPARGC1A
Entrez