PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

Images

 
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control ...read more
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control ...read more
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control ...read more
Genetic Strategies: Knockdown Validated: PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free [NB300-537] - PPAR alpha/NR1C1 was knocked down with siRNA in primary cultured rat cardiomyocytes. Image from verified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, Flow, GS, ICC/IF, IHC, IP, ChIP, KD
Clone
3B6/PPAR
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free Summary

Immunogen
Purified recombinant PPAR alpha protein.
Localization
Nuclear
Specificity
PPAR alpha (3B6/PPAR)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PPARA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 1:10-1:500
  • Flow Cytometry 1 ug / 10^6 cells
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/ Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Knockdown Validated
  • Western Blot 2 - 5 ug/ml
Application Notes
May be useful in ChIP. Rabbit-IP reported in publication, see Sumanasekera WK et al. Rat-IHC reported in publication, see Crespillo A et al.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB300-537 in the following applications:

Publications
Read Publications using
NB300-537 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PPAR alpha/NR1C1 Antibody (3B6/PPAR) - BSA Free

  • alpha
  • hPPAR
  • NR1C1
  • NR1C1MGC2237
  • Nuclear receptor subfamily 1 group C member 1
  • peroxisome proliferator-activated receptor alpha
  • PPAR alpha
  • PPARA
  • PPARalpha
  • PPAR-alpha
  • PPARMGC2452

Background

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR alpha/NR1C1 Antibody (NB300-537)(16)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 5 applications: IF/IHC, IHC-P, IP, Simple Western, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(2)
IP
(2)
Simple Western
(1)
WB
(9)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
Rat
(4)
Bovine
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB300-537 Applications Species
Abou-Rjeileh U, Dos Santos Neto JM, Chirivi M et al. Oleic acid abomasal infusion limits lipolysis and improves insulin sensitivity in adipose tissue from periparturient dairy cows Journal of dairy science 2023-04-25 [PMID: 37105874] (Simple Western, Bovine)

Details:
Dilutions 1:50
Simple Western Bovine
Liu B, Jie X, Deng J et al. Bupi Yishen formula may prevent kidney fibrosis by modulating fatty acid metabolism in renal tubules Phytomedicine : international journal of phytotherapy and phytopharmacology 2023-03-16 [PMID: 37001297] (IHC-P, Human, Rat) IHC-P Human, Rat
Chun-yang H, Shin-ichi O, Xiaoyong X et al. PERM1 regulates genes involved in fatty acid metabolism in the heart by interacting with PPAR alpha and PGC-1 alpha Scientific Reports 2022-08-26 [PMID: 36028747] (WB, Rat) WB Rat
Zhang F, Liu L, Xie Y et al. Cardiac contractility modulation ameliorates myocardial metabolic remodeling in a rabbit model of chronic heart failure through activation of AMPK and PPAR-? pathway Open Med (Wars) 2022-02-22 [PMID: 35799598] (WB) WB
Yang Q, Sun S, Liu W et al. Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice Biochemical and Biophysical Research Communications 2020-02-01 [PMID: 32122652] (WB, Mouse) WB Mouse
Kumar S, Rani R, Karns R, Gandhi CR. Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression FASEB J. 2018-12-12 [PMID: 30540918] (IHC-P, Mouse) IHC-P Mouse
Li L, He M, Xiao H et al. Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway. Cell Physiol Biochem 2018-03-06 [PMID: 29529605] (WB, Rat) WB Rat
Sun Q, Zhang W, Zhong W et al. Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function. Biochim Biophys Acta 2016-09-12 [PMID: 27634671] (WB, Mouse) WB Mouse
Rotondi S, Modarelli A, Oliva MA et al. Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells. Mol. Cell. Endocrinol. 2016-02-10 [PMID: 26872613] (WB, Rat) WB Rat
Tatone C, Benedetti E, Vitti M et al. Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells. J. Cell. Physiol. 2015-09-02 [PMID: 26332656] (WB, Human) WB Human
Show All 16 Publications.

Reviews for PPAR alpha/NR1C1 Antibody (NB300-537) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Rat and Rat primary cultured cardiomyocytes.

Reviews using NB300-537:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(1)
Rat and Rat primary cultured cardiomyocytes
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot PPAR alpha/NR1C1 NB300-537
Enlarge
4
reviewed by:
Shinichi Oka
WB Rat and Rat primary cultured cardiomyocytes 08/24/2022
View

Summary

ApplicationWestern Blot
Sample Testedprimary neonatal ventricular cardiomyocytes
SpeciesRat and Rat primary cultured cardiomyocytes
LotUB274777

Comments

CommentsEndogenous PPARa was knocked down with siRNA.
***Novus Innovators Program - new species or application used on a primary antibody.***
Western Blot PPAR alpha/NR1C1 NB300-537
Enlarge
5
reviewed by:
Verified Customer
WB Human 10/02/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
SpeciesHuman

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PPAR alpha/NR1C1 Antibody (NB300-537). (Showing 1 - 2 of 2 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. Would you so kind to let me have protocol of product which cat have cat number NB300-537?
    • We do not have any protocols specifically for product NB300-537. However, we do have some publications listed for this product on the datasheet under the "Images, Reviews & Publications" tab. Furthermore, our general protocols for a range of antibody applications can be found using this link.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Shinichi Oka
08/24/2022
Application: WB
Species: Rat and Rat primary cultured cardiomyocytes

Verified Customer
10/02/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol PPARA
Entrez
Uniprot