PPAR alpha/NR1C1 Antibody (3B6/PPAR)


Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on U-87 cells with PPAR alpha/NR1C1 Antibody [3B6/PPAR] NB300-537AF647 (blue) and a matched isotype control ...read more
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Hek293 cells with PPAR alpha/NR1C1 [3B6/PPAR] Antibody NB300-537AF647 (blue) and a matched isotype control ...read more
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control ...read more

Product Details

Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
1 mg/ml

PPAR alpha/NR1C1 Antibody (3B6/PPAR) Summary

Purified recombinant PPAR alpha protein.
PPAR alpha (3B6/PPAR)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1 ug / 10^6 cells
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 2 - 5 ug/ml
Application Notes
May be useful in ChIP. Rabbit-IP reported in publication, see Sumanasekera WK et al. Rat-IHC reported in publication, see Crespillo A et al.
Reviewed Applications
Read 1 Review rated 5
NB300-537 in the following applications:

Read Publications using
NB300-537 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • IP
    2 publications
  • WB
    7 publications

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified

Alternate Names for PPAR alpha/NR1C1 Antibody (3B6/PPAR)

  • alpha
  • hPPAR
  • NR1C1
  • NR1C1MGC2237
  • Nuclear receptor subfamily 1 group C member 1
  • peroxisome proliferator-activated receptor alpha
  • PPAR alpha
  • PPARalpha
  • PPAR-alpha
  • PPARMGC2452


Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: ELISA
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Species: Hu
Applications: ELISA
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Bv, Hu, Mu, Po, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
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Applications: DirELISA, IP, WB
Species: Hu
Applications: IHC, IP, WB
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
Species: Ch, Fe, Ha, Hu, Mu, Pl, Po, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KD, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for PPAR alpha/NR1C1 Antibody (NB300-537)(12)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 4 applications: IHC, IHC-P, IP, WB.

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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB300-537 Applications Species
Yang Q, Sun S, Liu W et al. Hypoxia training improves hepatic steatosis partly by downregulation of CB1 receptor in obese mice Biochemical and Biophysical Research Communications Feb 1 2020 [PMID: 32122652] (WB, Mouse) WB Mouse
Kumar S, Rani R, Karns R, Gandhi CR. Augmenter of liver regeneration protein deficiency promotes hepatic steatosis by inducing oxidative stress and microRNA-540 expression FASEB J. Dec 12 2018 [PMID: 30540918] (IHC-P, Mouse) IHC-P Mouse
Li L, He M, Xiao H et al. Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway. Cell Physiol Biochem 2018 Mar 06 [PMID: 29529605] (WB, Rat) WB Rat
Sun Q, Zhang W, Zhong W et al. Pharmacological inhibition of NOX4 ameliorates alcohol-induced liver injury in mice through improving oxidative stress and mitochondrial function. Biochim Biophys Acta 2016 Sep 12 [PMID: 27634671] (WB, Mouse) WB Mouse
Rotondi S, Modarelli A, Oliva MA et al. Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells. Mol. Cell. Endocrinol. 2016 Feb 10 [PMID: 26872613] (WB, Rat) WB Rat
Tatone C, Benedetti E, Vitti M et al. Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells. J. Cell. Physiol. 2015 Sep 02 [PMID: 26332656] (WB, Human) WB Human
Cao H, Wen G et al. Role of peroxisome proliferator-activated receptor alpha in atherosclerosis. Mol Med Rep Jan 5 2014 [PMID: 24604149] (IHC, Rabbit) IHC Rabbit
Gao W, Xu W, Li H. The expression and role of peroxisome proliferator-activated receptor alpha in atherosclerosis. Mol Med Rep 2013 Mar [PMID: 23337913] (Rabbit) Rabbit
Crespillo A, Suarez J et al. Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade. Biochem J;433(1):175-85. 2010 Dec 15. [PMID: 20955176] (WB, IHC) WB, IHC
Liu YY, Heymann RS et al. A mutant thyroid hormone receptor alpha antagonizes peroxisome proliferator-activated receptor alpha signaling in vivo and impairs fatty acid oxidation. Endocrinology;148(3):1206-17. 2007 Mar. [PMID: 17122078] (WB) WB
Show All 12 Publications.

Review for PPAR alpha/NR1C1 Antibody (NB300-537) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB300-537:
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Images Ratings Applications Species Date Details
Western Blot PPAR alpha/NR1C1 NB300-537
reviewed by:
Verified Customer
WB Human 10/02/2014


ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer


Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000


Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PPAR alpha/NR1C1 Antibody (NB300-537). (Showing 1 - 2 of 2 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. Would you so kind to let me have protocol of product which cat have cat number NB300-537?
    • We do not have any protocols specifically for product NB300-537. However, we do have some publications listed for this product on the datasheet under the "Images, Reviews & Publications" tab. Furthermore, our general protocols for a range of antibody applications can be found using this link.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-537AF350
Alexa Fluor 405 NB300-537AF405
Alexa Fluor 488 NB300-537AF488
Alexa Fluor 532 NB300-537AF532
Alexa Fluor 594 NB300-537AF594
Alexa Fluor 647 NB300-537AF647
Alexa Fluor 700 NB300-537AF700
Alexa Fluor 750 NB300-537AF750
Allophycocyanin NB300-537APC
Biotin NB300-537B
DyLight 350 NB300-537UV
DyLight 405 NB300-537V
DyLight 488 NB300-537G
DyLight 550 NB300-537R
DyLight 594 NB300-537DL594
DyLight 650 NB300-537C
DyLight 680 NB300-537FR
DyLight 755 NB300-537IR
FITC NB300-537F
HRP NB300-537H
Janelia Fluor 549 NB300-537JF549
Janelia Fluor 646 NB300-537JF646
PE NB300-537PE
PerCP NB300-537PCP

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Recent Reviews


Verified Customer
Application: WB
Species: Human


Gene Symbol PPARA