PPAR alpha/NR1C1 Antibody (3B6/PPAR)

Images

 
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in 3T3 cells compared to an isotype control (blue).
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Jurkat cells compared to an isotype control (blue).
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of PPAR alpha in Hela cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RbSpecies Glossary
Applications WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP
Clone
3B6/PPAR
Clonality
Monoclonal
Host
Mouse
Concentration
1 mg/ml

PPAR alpha/NR1C1 Antibody (3B6/PPAR) Summary

Immunogen
Purified recombinant PPAR alpha protein.
Localization
Nuclear
Specificity
PPAR alpha (3B6/PPAR)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PPARA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 2 - 5 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Flow Cytometry 1 ug / 10^6 cells
  • Gel Super Shift Assays 1:1 - 1:100
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:10 - 1:500
Application Notes
May be useful in ChIP. Rabbit-IP reported in publication, see Sumanasekera WK et al. Rat-IHC reported in publication, see Crespillo A et al.
Reviewed Applications
Read 1 Review rated 5
using
NB300-537 in the following applications:

Publications
Read Publications using
NB300-537 in the following applications:

  • IHC
    1 publication
  • IP
    2 publications
  • WB
    4 publications

Alternate Names for PPAR alpha/NR1C1 Antibody (3B6/PPAR)

  • alpha
  • hPPAR
  • NR1C1
  • NR1C1MGC2237
  • Nuclear receptor subfamily 1 group C member 1
  • peroxisome proliferator-activated receptor alpha
  • PPAR alpha/NR1C1
  • PPARA
  • PPARalpha
  • PPAR-alpha
  • PPARMGC2452

Background

Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. The corresponding gene for the PPAR alpha is NR1C1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP

Publications for PPAR alpha/NR1C1 Antibody (NB300-537)(7)

We have publications tested in 3 confirmed species: Human, Rat, Rabbit.

We have publications tested in 3 applications: IHC, IP, WB.


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IHC
(1)
IP
(2)
WB
(4)
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Showing Publications 1 - 7 of 7.
Publications using NB300-537 Applications Species
Rotondi S, Modarelli A, Oliva MA et al. Expression of Peroxisome Proliferator-Activated Receptor alpha (PPARalpha) in somatotropinomas: Relationship with Aryl hydrocarbon receptor Interacting Protein (AIP) and in vitro effects of fenofibrate in GH3 cells. Mol. Cell. Endocrinol. 2016 Feb 10 [PMID:26872613] (WB, Rat) WB Rat
Tatone C, Benedetti E, Vitti M et al. Modulating Intrafollicular Hormonal Milieu in Controlled Ovarian Stimulation: Insights From PPAR Expression in Human Granulosa Cells. J. Cell. Physiol. 2015 Sep 02 [PMID:26332656] (WB, Human) WB Human
Gao W, Xu W, Li H. The expression and role of peroxisome proliferator-activated receptor alpha in atherosclerosis. Mol Med Rep 2013 Mar [PMID:23337913] (Rabbit) Rabbit
Crespillo A, Suarez J et al. Expression of the cannabinoid system in muscle: effects of a high-fat diet and CB1 receptor blockade. Biochem J;433(1):175-85. 2010 Dec 15. [PMID:20955176] (WB, IHC) WB, IHC
Liu YY, Heymann RS et al. A mutant thyroid hormone receptor alpha antagonizes peroxisome proliferator-activated receptor alpha signaling in vivo and impairs fatty acid oxidation. Endocrinology;148(3):1206-17. 2007 Mar. [PMID:17122078] (WB) WB
Alwayn IP, Andersson C et al. Inhibition of matrix metalloproteinases increases PPAR-alpha and IL-6 and prevents dietary-induced hepatic steatosis and injury in a murine model. Am J Physiol Gastrointest Liver Physiol;291(6):G1011-9. 2006 Dec. [PMID:16844679] (IP) IP
Sumanasekera WK et al. Evidence that peroxisome proliferator-activated receptor alpha is complexed with the 90-kDa heat shock protein and the hepatitis virus B X-associated protein 2. J Biol Chem;278(7):4467-73. 2003 Feb 14. [PMID:12482853] (IP) IP

Reviews for PPAR alpha/NR1C1 Antibody (NB300-537) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB300-537:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PPAR alpha/NR1C1 NB300-537
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reviewed by:
Anonymous
WB Human 10/02/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 20 sec

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PPAR alpha/NR1C1 Antibody (NB300-537). (Showing 1 - 2 of 2 FAQs).

  1. Please differentiate me between PPAR and PGC clearly.i am confuse to know the differ these two. waiting for your reply
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.
  2. Would you so kind to let me have protocol of product which cat have cat number NB300-537?
    • We do not have any protocols specifically for product NB300-537. However, we do have some publications listed for this product on the datasheet under the "Images, Reviews & Publications" tab. Furthermore, our general protocols for a range of antibody applications can be found using this link.

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol PPARA
Entrez
Uniprot

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