OCT4 Antibody (NB100-2379)

Images

 
Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.
Flow Cytometry: OCT4 Antibody [NB100-2379] - Flow cytometry analysis of NTERA-2 cells using OCT4 antibody NB100-2379 at 1:50 dilution with detection employing Dylight-488 conjugated goat anti-rabbit secondary antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Po, Bv, FeSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

OCT4 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Porcine (93%), Feline (93%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU5F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
Application Notes
This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
OCT4 Lysate (NBL1-14620)
HeLa Lysate (NB800-PC1)
Control Peptide
OCT4 Peptide (NB100-2379PEP)
Publications
Read Publications using
NB100-2379 in the following applications:

Reactivity Notes

Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OCT4 Antibody

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • otc-4
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein

Background

OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2379
Species: Hu, Mu, Rt, Pm, Po, Bv, Fe
Applications: WB, Flow, ICC/IF

Publications for OCT4 Antibody (NB100-2379)(8)

We have publications tested in 3 confirmed species: Human, Rat, Bovine.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(5)
All Applications
Filter By Species
Human
(2)
Rat
(2)
Bovine
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-2379 Applications Species
Lupicka M, Socha B, Szczepanska A, Korzekwa A. Expression of pluripotency markers in the bovine uterus with adenomyosis. Reprod Biol Endocrinol 2015 [PMID: 26416515] (WB) WB
Lin HY, Lee DC, Wang HD et al. Activation of FGF1B Promoter and FGF1 Are Involved in Cardiogenesis Through the Signaling of PKC, but Not MAPK. Stem Cells Dev 2015 Dec 15 [PMID: 26414172]
Lu H Samanta D Xiang L et al. Chemotherapy triggers HIF-1-dependent glutathione synthesis and copper chelation that induces the breast cancer stem cell phenotype. Proc Natl Acad Sci U S A. 2015 Jul 30 [PMID: 26229077] (WB, Human) WB Human
Darekar SD, Mushtaq M, Gurrapu S et al. Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts. Oncotarget. 2015 May 12 [PMID: 26023799] (WB, Rat)

Details:
OCT4 antibody was used for WB analysis of lysates from primary rat skin fibroblasts /RSFs and immortalized cells namely clone 17, clone 13 and clone 6. Oct4 was found to be induced in all clones of immortalized RSFs compared with the parental cells (Figure 2d).
WB Rat
Liu JW, Hsu YC, Kao CY et al. LIF-induced Stat3 signaling suppresses FGF1-induced Erk1/2 activation to inhibit the downstream differentiation in mouse embryonic stem cells Stem Cells Dev 2012 Dec 4 [PMID: 23205673]
Ruggeri RR, Watanabe Y, Meirelles F et al. The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions J Assist Reprod Genet 2012 Sep 29 [PMID: 23054358] (ICC/IF, Bovine) ICC/IF Bovine
Cho YM, Kim YS, Kang MJ et al. Long-term recovery of irradiated prostate cancer increases cancer stem cells Prostate 2012 Dec 1 [PMID: 22513891] (WB, Human) WB Human
Yenamandra SP, Darekar SD, Kashuba V et al. Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts. Cell Death Dis 2012 Jan [PMID: 22258410] (WB, Rat) WB Rat

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Product General Protocols

View specific protocols for OCT4 Antibody (NB100-2379): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OCT4 Antibody (NB100-2379). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
    • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2379AF405
Alexa Fluor 488 Labeled NB100-2379AF488
Alexa Fluor 647 Labeled NB100-2379AF647
Alexa Fluor 700 Labeled NB100-2379AF700
Biotin Labeled NB100-2379B
DyLight 405 Labeled NB100-2379V
DyLight 488 Labeled NB100-2379G
DyLight 550 Labeled NB100-2379R
DyLight 650 Labeled NB100-2379C
FITC Labeled NB100-2379F
HRP Labeled NB100-2379H
PE Labeled NB100-2379PE

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Research Areas for OCT4 Antibody (NB100-2379)

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Blogs on OCT4.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol POU5F1
Entrez
Uniprot