OCT4 Antibody

Images

 
Western Blot: OCT4 Antibody [NB100-2379] - YM155 significantly reduced tumorsphere formation and inhibited EGFR autophosphorylation and G9a expression. YM155 is reported to suppress cancer stemness; therefore, we used ...read more
Western Blot: OCT4 Antibody [NB100-2379] - WB detection of OCT4 in mouse brain lysate using OCT4 antibody with ECL based detection. The antibody generated a specific band of OCT4 at the expected ~40kDa position.
Flow Cytometry: OCT4 Antibody [NB100-2379] - An intracellular stain was performed on Jurkat with NB100-2379 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells ...read more
Western Blot: OCT4 Antibody [NB100-2379] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and Western blot assay was performed. WB image submitted ...read more
Immunocytochemistry/ Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a DyLight 488 labeled secondary antibody.
Western Blot: OCT4 Antibody [NB100-2379] - The expression of pluripotency factors Oct4 in human head and neck cancer cell lines.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Pm, Po, Bv, FeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

OCT4 Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Porcine (93%), Bovine (93%), Feline (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
POU5F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
Application Notes
This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa. This OCT4 antibody is validated for Western blotting from verified customer review. Use in Immunohistochemistry reported in scientific literature (PMID: 30726734).
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
OCT4 Overexpression Lysate
HeLa Whole Cell Lysate
Control Peptide
OCT4 Peptide (NB100-2379PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-2379 in the following applications:

Publications
Read Publications using
NB100-2379 in the following applications:

  • 3 publications
  • 5 publications
  • IHC
    1 publication
  • KD
    1 publication
  • KO
    1 publication
  • WB
    8 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Cow (93%), Feline (93%), Porcine (93%). Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues. Bovine reactivity reported in scientific literature (PMID: 23054358).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15 M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OCT4 Antibody

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • otc-4
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein

Background

OCT4 (octamer-binding protein 4) is a transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and generates a trimeric complex with SOX2 on DNA for controlling the expression of several genes (YES1, FGF4, UTF1, ZFP206 etc.) involved in embryonic development. Expressed mainly in the nucleus, OCT4 interacts with UBE2I, ZSCAN10, PKM, WWP2 etc. and OCT4-PKM interaction positively regulate OCT4's transcriptional activity. Once translated, its modifications such as sumoylation enhances the protein stability, DNA binding and transactivation activity and is required for enhanced YES1 expression. OCT4 undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. OCT4 is found in oocytes and is zygotically expressed in pluripotent cells of pre-gastrulation embryos, and has been observed to be essential for early embryogenesis as well as for embryonic stem cell pluripotency. OCT4 is expressed in developing brain with highest levels in specific cell layers of the cortex, the olfactory bulb, hippocampus and cerebellum. Its expression decreases gradually after embryoid body formation, however, improper re-activation of OCT4 contributes to oncogenic processes and it has been implicated in certain malignancies such as breast and ovarian cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF1997
Species: Hu
Applications: WB, ChIP, ICC
H00388112-B01P
Species: Hu
Applications: WB
MAB2018
Species: Hu, Mu, Rt
Applications: WB, Simple Western, CyTOF-reported, ICC, ICFlow
NB600-302
Species: Hu, Mu, Dr
Applications: WB, Simple Western, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF3158
Species: Mu
Applications: WB, IHC, ChIP, ICC
NBP2-27406
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37357
Species: Hu, Mu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
MAB1259
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
AF1356
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
7460-F4/CF
Species: Hu
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
AF1759
Species: Hu, Mu
Applications: WB, Simple Western, IP, ChIP, ICC
AF3757
Species: Hu
Applications: WB, ICC
NB120-16518
Species: Hu, Mu, Rt, Po
Applications: WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready

Publications for OCT4 Antibody (NB100-2379)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 6 applications: Flow, ICC/IF, IHC, KD, KO, WB.


Filter By Application
Flow
(3)
ICC/IF
(5)
IHC
(1)
KD
(1)
KO
(1)
WB
(8)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
Rat
(3)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-2379 Applications Species
Yang J, Liu H, Sun H et al. Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach Stem Cell Res Aug 4 2020 [PMID: 32791484] (ICC/IF, Human) ICC/IF Human
Li W, Zhang N, Jin C et al. MUC1-C drives stemness in progression of colitis to colorectal cancer JCI Insight May 19 2020 [PMID: 32427590] (WB, KO, Mouse) WB, KO Mouse
Lu H, Xie Y, Tran L et al. Chemotherapy-induced S100A10 recruits KDM6A to facilitate OCT4-mediated breast cancer stemness J. Clin. Invest. May 19 2020 [PMID: 32427586] (WB, KD, Human) WB, KD Human
Yang T, Qin J, Zhang Q et Al. Generation of induced pluripotent stem cell line (ZZUi0018-A ) from a patient with spinocerebellar ataxia type 6 Stem Cell Res. [PMID: 32229428] (ICC/IF, Flow, Human) ICC/IF, Flow Human
Gao Y, Wang Y, Wang Z et al. Generation of induced pluripotent stem cell line (ZZUi0016-A) from dermal fibroblasts of a normal human Stem Cell Res. [PMID: 32007761] (ICC/IF, Flow, Human) ICC/IF, Flow Human
Sohni A, Tan K, Song HW et al. The Neonatal and Adult Human Testis Defined at the Single-Cell Level Cell Rep Feb 5 2019 [PMID: 30726734] (IHC, Human) IHC Human
Han YM, Bedarida T, Ding Y et al. B-Hydroxybutyrate Prevents Vascular Senescence through hnRNP A1-Mediated Upregulation of Oct4. Mol. Cell Sep 20 2018 [PMID: 30197300] (ICC/IF, Mouse) ICC/IF Mouse
Cheng CC, Chang J, Huang SC et al. YM155 as an inhibitor of cancer stemness simultaneously inhibits autophosphorylation of epidermal growth factor receptor and G9a-mediated stemness in lung cancer cells PLoS ONE 2017 Aug 08 [PMID: 28787001] (WB, Human) WB Human
McCulloh Christopher J, Olson Jacob K, Zhou Yu et al. Stem cells and necrotizing enterocolitis: A direct comparison of the efficacy of multiple types of stem cells. Journal of Pediatric Surgery 2018 Jun 1 [PMID: 28366560] (Flow, Rat) Flow Rat
Lupicka M, Socha B, Szczepanska A, Korzekwa A. Expression of pluripotency markers in the bovine uterus with adenomyosis. Reprod Biol Endocrinol 2015 [PMID: 26416515] (WB) WB
Show All 17 Publications.

Reviews for OCT4 Antibody (NB100-2379) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NB100-2379:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot OCT4 NB100-2379
Enlarge
5
reviewed by:
Anonymous
WB Human 05/18/2020
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
LotC-3
Western Blot OCT4 NB100-2379
Enlarge
5
reviewed by:
Anonymous
WB Human 10/11/2018
View

Summary

ApplicationWestern Blot
Sample Testedhuman head and neck cancer cell lines
SpeciesHuman
LotC-3

Product General Protocols

View specific protocols for OCT4 Antibody (NB100-2379): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OCT4 Antibody (NB100-2379). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for the following markers to use to stain cells derived from both human and mouse embryonic and induced pluripotent stem cells: undifferentiated cells, neurons, oligodendrocytes, and astrocytes.
    • For ES markers some popular targets are OCT4 (NB100-2379), KLF4 (NBP2-17070) and SOX2 (NB110-37235). PEA15 is expressed in Astrocytes and this might be one of interest for you (NBP1-61390). OCT4 is one that can also be expressed in Oligodendrocytes.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2379AF350
Alexa Fluor 405 NB100-2379AF405
Alexa Fluor 488 NB100-2379AF488
Alexa Fluor 532 NB100-2379AF532
Alexa Fluor 594 NB100-2379AF594
Alexa Fluor 647 NB100-2379AF647
Alexa Fluor 700 NB100-2379AF700
Alexa Fluor 750 NB100-2379AF750
Biotin NB100-2379B
DyLight 350 NB100-2379UV
DyLight 405 NB100-2379V
DyLight 488 NB100-2379G
DyLight 550 NB100-2379R
DyLight 594 NB100-2379PD6
DyLight 650 NB100-2379C
DyLight 680 NB100-2379FR
DyLight 755 NB100-2379IR
FITC NB100-2379F
HRP NB100-2379H
Janelia Fluor 549 NB100-2379JF549
Janelia Fluor 646 NB100-2379JF646
PE NB100-2379PE

Additional OCT4 Products

Bioinformatics Tool for OCT4 Antibody (NB100-2379)

Discover related pathways, diseases and genes to OCT4 Antibody (NB100-2379). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for OCT4 Antibody (NB100-2379)

Discover more about diseases related to OCT4 Antibody (NB100-2379).
 

Pathways for OCT4 Antibody (NB100-2379)

View related products by pathway.

PTMs for OCT4 Antibody (NB100-2379)

Learn more about PTMs related to OCT4 Antibody (NB100-2379).
 

Research Areas for OCT4 Antibody (NB100-2379)

Find related products by research area.

Blogs on OCT4.

Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

Stemness for Surviving Hypoxia: TGF-beta/Smad Signaling in Multiple Myeloma
By Jamshed Arslan Pharm.D. Multiple myeloma (MM) is a cancer of antibody-producing plasma cells. The bone marrow (BM) of MM patients is hypoxic, and MM cells overexpress many cancerous genes that are regulated by hy...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
05/18/2020
Application: WB
Species: Human

Anonymous
10/11/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol POU5F1
Entrez
Uniprot