CD117/c-kit Antibody [Unconjugated]

Images

 
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

CD117/c-kit Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Specificity
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KIT
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 3 Reviews rated 3
using
AF1356 in the following applications:

Publications
Read Publications using
AF1356 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (AF1356)(17)

We have publications tested in 2 confirmed species: Mouse, Porcine.

We have publications tested in 10 applications: Flow, ICC, ICC Fresh, IHC, IHC - Paraffin embedded, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, WB.


Filter By Application
Flow
(1)
ICC
(3)
ICC Fresh
(1)
IHC
(3)
IHC - Paraffin embedded
(1)
IHC Frozen
(2)
IHC Not Specified
(1)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(3)
WB
(1)
All Applications
Filter By Species
Mouse
(16)
Porcine
(1)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using AF1356 Applications Species
A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair Cell Res., 2017;0(0):. 2017 [PMID: 28621328] (ICC,, Mouse) ICC Mouse
MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures PLoS ONE, 2017;12(4):e0176031. 2017 [PMID: 28426719] (IHC Frozen,, Mouse) IHC Frozen Mouse
Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice PLoS ONE, 2016;11(5):e0152179. 2016 [PMID: 27195709] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis. PLoS ONE, 2015;10(9):e0138621. 2015 [PMID: 26389592] (IHC OCT-embedded,, Mouse) IHC OCT-embedded Mouse
Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment. J Biol Chem, 2015;290(42):25411-26. 2015 [PMID: 26324717] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium. PLoS ONE, 2015;10(3):e0119718. 2015 [PMID: 25803307] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth. Mucosal Immunol, 2015;8(5):1047-59. 2015 [PMID: 25603827] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. Nat Protoc, 2014;9(7):1662-81. 2014 [PMID: 24945383] (ICC Fresh,, Mouse) ICC Fresh Mouse
van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature, 2014;0(0):. 2014 [PMID: 24805242] (WB,, Mouse) WB Mouse
Toko, Haruhiro, Hariharan, Nirmala, Konstandin, Mathias, Ormachea, Lucia, McGregor, Michael, Gude, Natalie, Sundararaman, Balaji, Joyo, Eri, Joyo, Anya Y, Collins, Brett, Din, Shabana, Mohsin, Sadia, Uchida, Takafumi, Sussman, Mark A Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells. J Biol Chem, 2014;289(9):5348-56. 2014 [PMID: 24375406] (IHC,, Mouse) IHC Mouse
Show All 17 Publications.

Reviews for CD117/c-kit Antibody (AF1356) (3) 33

Average Rating: 3
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Mouse.

Reviews using AF1356:
Filter by Applications
WB
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Comments

CommentsPublished See PMID 24375406
 
reviewed by:
12/31/1969
View
 
reviewed by:
Anonymous
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Product General Protocols

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FAQs for CD117/c-kit Antibody (AF1356) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for CD117/c-kit Antibody (AF1356)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (AF1356). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

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Recent Reviews

3
5
1
4
1
3
0
2
0
1
0
1

 
Anonymous
01/05/2015
Application: WB
Species: Mouse

 

12/31/1969
Application:
Species:

 
Anonymous
01/05/2015
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol KIT
Entrez
Uniprot