CD117/c-kit Antibody [Unconjugated]

Images

 
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. ...read more
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CD117/c-kit Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Specificity
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KIT
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF1356 in the following applications:

Publications
Read Publications using
AF1356 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (AF1356)(55)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Porcine, Transgenic Mouse.

We have publications tested in 8 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(5)
ICC
(10)
IF
(1)
IHC
(27)
IHC-Fr
(2)
IHC-P
(7)
Neutralization
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(8)
Mouse
(47)
Rat
(1)
Porcine
(1)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using AF1356 Applications Species
Nguyen, VTT;Taheri, N;Choi, EL;Kellogg, TA;Linden, DR;Hayashi, Y; Insulin-like growth factor1 preserves gastric pacemaker cells and motor function in aging via ERK1/2 activation Cellular and molecular gastroenterology and hepatology 2023-06-08 [PMID: 37301443] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Nakajima-Takagi, Y;Oshima, M;Takano, J;Koide, S;Itokawa, N;Uemura, S;Yamashita, M;Andoh, S;Aoyama, K;Isshiki, Y;Shinoda, D;Saraya, A;Arai, F;Yamaguchi, K;Furukawa, Y;Koseki, H;Ikawa, T;Iwama, A; Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis eLife 2023-06-02 [PMID: 37266576] (IHC, Transgenic Mouse, Mouse) IHC Transgenic Mouse, Mouse
Hao, X;Shen, Y;Chen, N;Zhang, W;Valverde, E;Wu, L;Chan, HL;Xu, Z;Yu, L;Gao, Y;Bado, I;Michie, LN;Rivas, CH;Dominguez, LB;Aguirre, S;Pingel, BC;Wu, YH;Liu, F;Ding, Y;Edwards, DG;Liu, J;Alexander, A;Ueno, NT;Hsueh, PR;Tu, CY;Liu, LC;Chen, SH;Hung, MC;Lim, B;Zhang, XH; Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removal Cell stem cell 2023-05-04 [PMID: 37146584] (IHC, Mouse) IHC Mouse
A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse Nature Communications, 2022-12-21;13(1):7860. 2022-12-21 [PMID: 36543770] (IHC, Mouse) IHC Mouse
T Klein-Rode, K Micklich, A Sanz-Moren, M Tost, J Calzada-Wa, T Adler, M Klaften, S Sabrautzki, B Aigner, M Kraiger, V Gailus-Dur, H Fuchs, German Mou, A Gründer, H Pahl, E Wolf, M Hrabe de A, B Rathkolb New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance Scientific Reports, 2022-11-17;12(1):19793. 2022-11-17 [PMID: 36396684] (IHC, Mouse) IHC Mouse
Z Zheng, H He, XT Tang, H Zhang, F Gou, H Yang, J Cao, S Shi, Z Yang, G Sun, X Xie, Y Zeng, A Wen, Y Lan, J Zhou, B Liu, BO Zhou, T Cheng, H Cheng Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis Cell Stem Cell, 2022-11-03;29(11):1562-1579.e7. 2022-11-03 [PMID: 36332570] (Flow Cytometry, Human) Flow Cytometry Human
S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells JCI Insight, 2022-03-08;7(5):. 2022-03-08 [PMID: 35260534] (IHC, Mouse) IHC Mouse
J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021-11-29;12(1):6963. 2021-11-29 [PMID: 34845225] (Flow Cytometry, Mouse) Flow Cytometry Mouse
IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling Cell Reports, 2021-11-23;37(8):110036. 2021-11-23 [PMID: 34818550] (Western Blot, Human) Western Blot Human
A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 2021-10-26;37(4):109885. 2021-10-26 [PMID: 34706238] (IHC, Mouse) IHC Mouse
Show All 55 Publications.

Reviews for CD117/c-kit Antibody (AF1356) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF1356:
Filter by Applications
WB
(2)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 03/23/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse es cells
SpeciesMouse
LotIEO0217011
  5
reviewed by:
Verified Customer
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24375406
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22262798
SpeciesMouse
  4
reviewed by:
Verified Customer
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24375406
SpeciesMouse

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FAQs for CD117/c-kit Antibody (AF1356) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for CD117/c-kit Antibody (AF1356)

Discover related pathways, diseases and genes to CD117/c-kit Antibody (AF1356). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Discover more about diseases related to CD117/c-kit Antibody (AF1356).
 

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

 
Verified Customer
03/23/2018
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KIT
Uniprot