CD117/c-kit Antibody [Unconjugated]

Images

 
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. ...read more
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CD117/c-kit Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Specificity
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human CD117 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KIT
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Simple Western 5 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF1356 in the following applications:

Publications
Read Publications using
AF1356 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (AF1356)(27)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 11 applications: Flow, ICC, ICC Fresh, IHC, IHC - Paraffin embedded, IHC Frozen, IHC Not Specified, IHC OCT-embedded, IHC Paraffin-embedded, IHC-P, WB.


Filter By Application
Flow
(1)
ICC
(7)
ICC Fresh
(1)
IHC
(6)
IHC - Paraffin embedded
(1)
IHC Frozen
(2)
IHC Not Specified
(1)
IHC OCT-embedded
(1)
IHC Paraffin-embedded
(3)
IHC-P
(2)
WB
(2)
All Applications
Filter By Species
Human
(2)
Mouse
(24)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF1356 Applications Species
D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells Int Heart J, 2018;59(1):180-189. 2018 [PMID: 29375111] (ICC,, Mouse) ICC Mouse
S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis PLoS ONE, 2018;13(1):e0190925. 2018 [PMID: 29324788] (IHC-P,, Mouse) IHC-P Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC,, Mouse) IHC Mouse
Y Kawai, A Oda, Y Kanai, R Goitsuka Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis PLoS ONE, 2018;13(1):e0190702. 2018 [PMID: 29293683] (IHC-P,, Mouse) IHC-P Mouse
JC Harrell, TM Shroka, BM Jacobsen Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer Oncogenesis, 2017;6(11):2. 2017 [PMID: 29176653] (WB,, Human) WB Human
A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair Cell Res., 2017;0(0):. 2017 [PMID: 28621328] (ICC,, Mouse) ICC Mouse
C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll A transit-amplifying population underpins the efficient regenerative capacity of the testis J. Exp. Med., 2017;0(0):. 2017 [PMID: 28461596] (ICC,, Mouse) ICC Mouse
MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy Circ. Res., 2017;0(0):. 2017 [PMID: 28446444] (ICC,, Human) ICC Human
MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures PLoS ONE, 2017;12(4):e0176031. 2017 [PMID: 28426719] (IHC Frozen,, Mouse) IHC Frozen Mouse
L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice Circ. Res, 2017;0(0):. 2017 [PMID: 28298296] (IHC,, Mouse) IHC Mouse
Show All 27 Publications.

Reviews for CD117/c-kit Antibody (AF1356) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF1356:
Filter by Applications
WB
(2)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 03/23/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse es cells
SpeciesMouse
LotIEO0217011
 
reviewed by:
Anonymous
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Comments

CommentsPublished See PMID 24375406
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22262798
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22262798

Details

Detection NotesSee PMID 22262798

Comments

Comments"With 2nd Ab: AlexaFluor 546-conjugated donkey anti-goat IgG (Molecular probes A11056)"See PMID 22262798
 
reviewed by:
Anonymous
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Product General Protocols

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FAQs for CD117/c-kit Antibody (AF1356) (0)

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Diseases for CD117/c-kit Antibody (AF1356)

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Blogs on CD117/c-kit. Showing 1-10 of 49 blog posts - Show all blog posts.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene...  Read full blog post.

Showing 1-10 of 49 blog posts - Show all blog posts.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

 
Anonymous
03/23/2018
Application: WB
Species: Mouse

 
Anonymous
01/05/2015
Application: WB
Species: Mouse

 
Anonymous
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KIT
Entrez
Uniprot