CD117/c-kit Antibody [Unconjugated]

CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD117/c-kit at approximately 135, 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit at approximately 150-165 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CD117/c-kit Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
  Gln25-Thr519 (Ala207Glu)
  Accession # P05532
• Specificity: Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KIT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD117/c-kit Antibody (AF1356)(53)

Publications using AF1356

• Hao, X;Shen, Y;Chen, N;Zhang, W;Valverde, E;Wu, L;Chan, HL;Xu, Z;Yu, L;Gao, Y;Bado, I;Michie, LN;Rivas, CH;Dominguez, LB;Aguirre, S;Pingel, BC;Wu, YH;Liu, F;Ding, Y;Edwards, DG;Liu, J;Alexander, A;Ueno, NT;Hsueh, PR;Tu, CY;Liu, LC;Chen, SH;Hung, MC;Lim, B;Zhang, XH; Osteoprogenitor-GMP crosstalk underpins solid tumor-induced systemic immunosuppression and persists after tumor removal Cell stem cell 2023-05-04 [PMID: 37146584] (IHC, Mouse)
• A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse Nature Communications, 2022-12-21;13(1):7860. 2022-12-21 [PMID: 36543770] (IHC, Mouse)
• T Klein-Rode, K Micklich, A Sanz-Moren, M Tost, J Calzada-Wa, T Adler, M Klaften, S Sabrautzki, B Aigner, M Kraiger, V Gailus-Dur, H Fuchs, German Mou, A Gründer, H Pahl, E Wolf, M Hrabe de A, B Rathkolb New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance Scientific Reports, 2022-11-17;12(1):19793. 2022-11-17 [PMID: 36396684] (IHC, Mouse)
• Z Zheng, H He, XT Tang, H Zhang, F Gou, H Yang, J Cao, S Shi, Z Yang, G Sun, X Xie, Y Zeng, A Wen, Y Lan, J Zhou, B Liu, BO Zhou, T Cheng, H Cheng Uncovering the emergence of HSCs in the human fetal bone marrow by single-cell RNA-seq analysis Cell Stem Cell, 2022-11-03;29(11):1562-1579.e7. 2022-11-03 [PMID: 36332570] (Flow Cytometry, Human)
• S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells JCI Insight, 2022-03-08;7(5):. 2022-03-08 [PMID: 35260534] (IHC, Mouse)
• J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021-11-29;12(1):6963. 2021-11-29 [PMID: 34845225] (Flow Cytometry, Mouse)
• IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling Cell Reports, 2021-11-23;37(8):110036. 2021-11-23 [PMID: 34818550] (Western Blot, Human)
• A Heinrich, B Bhandary, SJ Potter, N Ratner, T DeFalco Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesis Cell Reports, 2021-10-26;37(4):109885. 2021-10-26 [PMID: 34706238] (IHC, Mouse)
• F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021-10-20;599(7883):125-130. 2021-10-20 [PMID: 34671159] (IHC, Mouse)
• K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo Bone Reports, 2021-09-29;15(0):101133. 2021-09-29 [PMID: 34632004] (IHC, Mouse)
• T Aschacher, K Schmidt, O Aschacher, E Eichmair, U Baranyi, B Winkler, M Grabenwoeg, A Spittler, F Enzmann, B Messner, J Riebandt, G Laufer, M Bergmann, M Ehrlich Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression Journal of Cellular and Molecular Medicine, 2021-09-25;0(0):. 2021-09-25 [PMID: 34562312] (Flow Cytometry, Human)
• K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021-04-07;146(3):125-135. 2021-04-07 [PMID: 34030795] (IHC, Rat)
• W Jia, L Kong, H Kidoya, H Naito, F Muramatsu, Y Hayashi, HY Hsieh, D Yamakawa, DK Hsu, FT Liu, N Takakura Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells Nature Communications, 2021-04-09;12(1):2118. 2021-04-09 [PMID: 33837181] (IHC, Mouse)
• S Suzuki, JR McCarrey, BP Hermann An mTORC1-dependent switch orchestrates the transition between mouse spermatogonial stem cells and clones of progenitor spermatogonia Cell Reports, 2021-02-16;34(7):108752. 2021-02-16 [PMID: 33596419] (Flow Cytometry, ICC, Mouse)
• K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules Scientific Reports, 2021-01-13;11(1):1110. 2021-01-13 [PMID: 33441739] (IHC, Mouse)
• T Su, Y Yang, S Lai, J Jeong, Y Jung, M McConnell, T Utsumi, Y Iwakiri Single-cell transcriptomics reveals zone-specific alterations of liver sinusoidal endothelial cells in cirrhosis Cellular and Molecular Gastroenterology and Hepatology, 2020-12-16;0(0):. 2020-12-16 [PMID: 33340713] (IHC, Mouse)
• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)
• EI Crosse, S Gordon-Key, S Rybtsov, A Binagui-Ca, H Felchle, NC Nnadi, K Kirschner, T Chandra, S Tamagno, DJ Webb, F Rossi, RA Anderson, A Medvinsky Multi-layered Spatial Transcriptomics Identify Secretory Factors Promoting Human Hematopoietic Stem Cell Development Cell Stem Cell, 2020-09-17;0(0):. 2020-09-17 [PMID: 32946788] (IHC, Human)
• C Sogawa-Fuj, A Hanagata, Y Fujiwara, Y Ishida, H Tomiyasu, T Kunieda, H Nakatomi, M Hori Defective development and microcirculation of intestine in Npr2 mutant mice Sci Rep, 2020-09-08;10(1):14761. 2020-09-08 [PMID: 32901096] (IHC, Western Blot, Mouse)
• DE Ebeid, F Firouzi, CY Esquer, JM Navarrete, BJ Wang, NA Gude, MA Sussman PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression Cells, 2020-08-31;9(9):. 2020-08-31 [PMID: 32878131] (Western Blot, Mouse)
• Y Hayashi, DT Asuzu, MR Bardsley, GB Gajdos, SM Kvasha, DR Linden, RA Nagy, SA Saravanape, SA Syed, Y Toyomasu, H Yan, EN Chini, SJ Gibbons, TA Kellogg, K Khazaie, M Kuro-O, JM Espindola, MP Singh, JG Tidball, M Wehling-He, G Farrugia, T Ordog Wnt-induced, TRP53-mediated Cell Cycle Arrest of Precursors Underlies Interstitial Cell of Cajal Depletion during Aging Cell Mol Gastroenterol Hepatol, 2020-08-07;0(0):. 2020-08-07 [PMID: 32771388] (Western Blot, Human, Mouse)
• MM Mikedis, Y Fan, PK Nicholls, T Endo, EK Jackson, SA Cobb, DG de Rooij, DC Page DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors Elife, 2020-07-20;9(0):. 2020-07-20 [PMID: 32686646] (IHC-P, Mouse)
• A Heinrich, SJ Potter, L Guo, N Ratner, T DeFalco Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and Spermatogenesis Cell Rep, 2020-04-14;31(2):107513. 2020-04-14 [PMID: 32294451] (IF, Mouse)
• M Hou, J Han, G Li, MY Kwon, J Jiang, S Emani, ES Taglauer, JA Park, EB Choi, M Vodnala, YW Fong, SM Emani, IO Rosas, MA Perrella, X Liu Multipotency of mouse trophoblast stem cells Stem Cell Res Ther, 2020-02-13;11(1):55. 2020-02-13 [PMID: 32054514] (ICC, IHC, Human, Mouse)
• P Agarwal, S Isringhaus, H Li, AJ Paterson, J He, Á Gomariz, T Nagasawa, C Nombela-Ar, R Bhatia Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells Cell Stem Cell, 2019-03-21;0(0):. 2019-03-21 [PMID: 30905620] (Neutralization, Mouse)
• H Kidoya, F Muramatsu, T Shimamura, W Jia, T Satoh, Y Hayashi, H Naito, Y Kunisaki, F Arai, M Seki, Y Suzuki, T Osawa, S Akira, N Takakura Regnase-1-mediated post-transcriptional regulation is essential for hematopoietic stem and progenitor cell homeostasis Nat Commun, 2019-03-06;10(1):1072. 2019-03-06 [PMID: 30842549] (IHC, Mouse)
• D Xie, Y Liao, B Wu, Y Chen, W Lin, D Lu, S Gao, S Zhu, C Peng, MH Jiang Cardiac Nestin+ Cells Derived from Early Stage of Dilated Cardiomyopathy Enhanced the Survival of the Doxorubicin-Injured Cardiac Muscle HL-1 Cells Int Heart J, 2018-01-01;59(1):180-189. 2018-01-01 [PMID: 29375111] (ICC, IHC, Mouse)
• S Saeidi, F Shapouri, RU de Iongh, F Casagranda, JM Sutherland, PS Western, EA McLaughlin, M Familari, GR Hime Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis PLoS ONE, 2018-01-11;13(1):e0190925. 2018-01-11 [PMID: 29324788] (IHC-P, Mouse)
• DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2017-11-13;15(1):39-46. 2017-11-13 [PMID: 29320487] (IHC, Mouse)
• Y Kawai, A Oda, Y Kanai, R Goitsuka Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis PLoS ONE, 2018-01-02;13(1):e0190702. 2018-01-02 [PMID: 29293683] (IHC-P, Mouse)
• JC Harrell, TM Shroka, BM Jacobsen Estrogen induces c-Kit and an aggressive phenotype in a model of invasive lobular breast cancer Oncogenesis, 2017-11-27;6(11):2. 2017-11-27 [PMID: 29176653] (Western Blot, Human)
• A Magadum, Y Ding, L He, T Kim, MD Vasudevara, Q Long, K Yang, N Wickramasi, HV Renikunta, N Dubois, G Weidinger, Q Yang, FB Engel Live cell screening platform identifies PPAR? as a regulator of cardiomyocyte proliferation and cardiac repair Cell Res., 2017-06-16;0(0):. 2017-06-16 [PMID: 28621328] (ICC, Mouse)
• C Carrieri, S Comazzetto, A Grover, M Morgan, A Buness, C Nerlov, D O'Carroll A transit-amplifying population underpins the efficient regenerative capacity of the testis J. Exp. Med., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461596] (ICC, Mouse)
• MM Monsanto, KS White, T Kim, BJ Wang, K Fisher, K Ilves, FG Khalafalla, A Casillas, K Broughton, S Mohsin, WP Dembitsky, MA Sussman Concurrent Isolation of Three Distinct Cardiac Stem Cell Populations from a Single Human Heart Biopsy Circ. Res., 2017-04-26;0(0):. 2017-04-26 [PMID: 28446444] (ICC, Human)
• MY Lee, SE Ha, C Park, PJ Park, R Fuchs, L Wei, BG Jorgensen, D Redelman, SM Ward, KM Sanders, S Ro Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures PLoS ONE, 2017-04-20;12(4):e0176031. 2017-04-20 [PMID: 28426719] (IHC-Fr, Mouse)
• L Luo, J Tang, K Nishi, C Yan, PU Dinh, J Cores, T Kudo, J Zhang, TS Li, K Cheng Fabrication of Synthetic Mesenchymal Stem Cells for the Treatment of Acute Myocardial Infarction in Mice Circ. Res, 2017-03-15;0(0):. 2017-03-15 [PMID: 28298296] (IHC, Mouse)
• AL Feng, YY Xiang, L Gui, G Kaltsidis, Q Feng, WY Lu Paracrine GABA and insulin regulate pancreatic alpha cell proliferation in a mouse model of type 1 diabetes Diabetologia, 2017-03-09;0(0):. 2017-03-09 [PMID: 28280900] (IHC, Mouse)
• Radiation Exposure Decreases the Quantity and Quality of Cardiac Stem Cells in Mice PLoS ONE, 2016-05-19;11(5):e0152179. 2016-05-19 [PMID: 27195709] (IHC, Mouse)
• Liu Q, Yang R, Huang X, Zhang H, He L, Zhang L, Tian X, Nie Y, Hu S, Yan Y, Zhang L, Qiao Z, Wang Q, Lui K, Zhou B Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes. Cell Res, 2015-12-04;26(1):119-30. 2015-12-04 [PMID: 26634606] (ICC, Mouse)
• Tanaka Y, Inoue-Yokoo T, Kulkeaw K, Yanagi-Mizuochi C, Shirasawa S, Nakanishi Y, Sugiyama D Embryonic Hematopoietic Progenitor Cells Reside in Muscle before Bone Marrow Hematopoiesis. PLoS ONE, 2015-09-21;10(9):e0138621. 2015-09-21 [PMID: 26389592] (IHC, Mouse)
• Quijada P, Hariharan N, Cubillo J, Bala K, Emathinger J, Wang B, Ormachea L, Bers D, Sussman M, Poizat C Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment. J Biol Chem, 2015-08-31;290(42):25411-26. 2015-08-31 [PMID: 26324717] (IHC-P, Mouse)
• Iglesias J, Cairney C, Ferrier R, McDonald L, Soady K, Kendrick H, Pringle M, Morgan R, Martin F, Smalley M, Blyth K, Stein T Annexin A8 identifies a subpopulation of transiently quiescent c-kit positive luminal progenitor cells of the ductal mammary epithelium. PLoS ONE, 2015-03-24;10(3):e0119718. 2015-03-24 [PMID: 25803307] (IHC-P, Mouse)
• Shiu J, Piazuelo M, Ding H, Czinn S, Drakes M, Banerjee A, Basappa N, Kobayashi K, Fricke W, Blanchard T Gastric LTi cells promote lymphoid follicle formation but are limited by IRAK-M and do not alter microbial growth. Mucosal Immunol, 2015-01-21;8(5):1047-59. 2015-01-21 [PMID: 25603827] (IHC-P, Mouse)
• Smith A, Lewis F, Aquila I, Waring C, Nocera A, Agosti V, Nadal-Ginard B, Torella D, Ellison G Isolation and characterization of resident endogenous c-Kit+ cardiac stem cells from the adult mouse and rat heart. Nat Protoc, 2014-06-19;9(7):1662-81. 2014-06-19 [PMID: 24945383] (ICC, Mouse)
• van Berlo J, Kanisicak O, Maillet M, Vagnozzi R, Karch J, Lin S, Middleton R, Marban E, Molkentin J c-kit+ cells minimally contribute cardiomyocytes to the heart. Nature, 2014-05-07;0(0):. 2014-05-07 [PMID: 24805242] (Western Blot, Mouse)
• Toko , Haruhiro, Hariharan , Nirmala, Konstandin , Mathias, Ormachea , Lucia, McGregor , Michael, Gude , Natalie, Sundararaman , Balaji, Joyo , Eri, Joyo , Anya Y, Collins , Brett, Din , Shabana, Mohsin , Sadia, Uchida , Takafumi, Sussman , Mark A Differential regulation of cellular senescence and differentiation by prolyl isomerase Pin1 in cardiac progenitor cells. J Biol Chem, 2013-12-27;289(9):5348-56. 2013-12-27 [PMID: 24375406] (IHC, Mouse)
• Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair. Circulation, 2013-09-10;128(11):S32-41. 2013-09-10 [PMID: 24030419] (IHC-P, Porcine)
• Fang , Shentong, Wei , Jing, Pentinmikko , Nalle, Leinonen , Hannele, Salven , Petri Generation of Functional Blood Vessels from a Single c-kit+ Adult Vascular Endothelial Stem Cell. PLoS Biol, 2012-10-16;10(10):e1001407. 2012-10-16 [PMID: 23091420] (IHC, Mouse)
• Nakamura-Ishizu A, Okuno Y, Omatsu Y, Okabe K, Morimoto J, Uede T, Nagasawa T, Suda T, Kubota Y Extracellular matrix protein tenascin-C is required in the bone marrow microenvironment primed for hematopoietic regeneration. Blood, 2012-05-02;119(23):5429-37. 2012-05-02 [PMID: 22553313] (IHC-Fr, Mouse)
• Moon TC, Yoshimura T, Parsons T, Befus AD Microenvironmental regulation of inducible nitric oxide synthase expression and nitric oxide production in mouse bone marrow-derived mast cells. J. Leukoc. Biol., 2012-01-18;91(4):581-90. 2012-01-18 [PMID: 22262798] (ICC, Mouse)
• Miyamoto K, Yoshida S, Kawasumi M Osteoclasts are dispensable for hematopoietic stem cell maintenance and mobilization. J. Exp. Med., 2011-10-17;208(11):2175-81. 2011-10-17 [PMID: 22006978] (IHC, Mouse)
• Fifadara NH, Aye CC, Raghuwanshi SK, Richardson RM, Ono SJ CCR1 expression and signal transduction by murine BMMC results in secretion of TNF-alpha, TGFbeta-1 and IL-6. Int. Immunol., 2009-07-10;21(8):991-1001. 2009-07-10 [PMID: 19592420] (Flow Cytometry, Mouse)
• Shin JS, Shelburne CP, Jin C, LeFurgey EA, Abraham SN Harboring of particulate allergens within secretory compartments by mast cells following IgE/FcepsilonRI-lipid raft-mediated phagocytosis. J. Immunol., 2006-11-01;177(9):5791-800. 2006-11-01 [PMID: 17056503] (ICC, Mouse)

Reviews for CD117/c-kit Antibody (AF1356) (4)

Average Rating: 4.3

(Based on 4 reviews)

We have 4 reviews tested in 1 species: Mouse.

reviewed by: Chiara Chiereghin WB Mouse 03/23/2018

Summary

• Application: Western Blot
• Sample Tested: mouse es cells
• Species: Mouse
• Lot: IEO0217011

reviewed by: Anonymous - WB Mouse 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 24375406
• Species: Mouse

reviewed by: Anonymous - IF Mouse 01/05/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22262798
• Species: Mouse

reviewed by: Anonymous - ICC Mouse 01/05/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 24375406
• Species: Mouse

Bioinformatics

• Gene Symbol: KIT
• Uniprot:
  • P05532()