CD117/c-kit Antibody [Unconjugated]

Images

 
Western blot shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line, and MC/9‑2 mouse mast cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse ...read more
Simple Western lane view shows lysates of MO7e human megakaryocytic leukemic cell line, P815 mouse mastocytoma cell line and MC/9-2 mouse mast cell line, loaded at 0.2 mg/mL. A specific band was detected for CD117/c-kit ...read more
CD117/c-kit was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human/Mouse CD117/c-kit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1356) at 15 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CD117/c-kit Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit
Gln25-Thr519 (Ala207Glu)
Accession # P05532
Specificity
Detects human and mouse CD117/c-kit in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KIT
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 5 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 4 Reviews rated 4.3
using
AF1356 in the following applications:

Publications
Read Publications using
AF1356 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD117/c-kit Antibody [Unconjugated]

  • CD117 antigen
  • CD117
  • ckit
  • c-kit
  • EC 2.7.10
  • EC 2.7.10.1
  • KIT
  • PBT
  • piebald trait
  • Proto-oncogene c-Kit
  • proto-oncogene tyrosine-protein kinase Kit
  • SCF R
  • SCFR
  • SCFRmast/stem cell growth factor receptor
  • soluble KIT variant 1
  • Tyrosine-protein kinase Kit
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
  • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem cell factor receptor (CD117, the gene product of the c-kit protooncogene) and its ligand, stem cell factor (also named c-kit ligand, mast cell growth factor), play essential roles in gametogenesis, melanogenesis and hematopoiesis. It is a transmembrane tyrosine kinase that is expressed on endothelial cells, mast cells, megakaryocytes, stem cells and multiple embryonic cells, such as melanoblasts and primordial germ cells. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD117/c-kit Antibody (AF1356)(46)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 7 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(9)
IHC
(16)
IHC-Fr
(2)
IHC-P
(7)
Neutralization
(1)
Western Blot
(7)
All Applications
Filter By Species
Human
(7)
Mouse
(36)
Rat
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using AF1356 Applications Species
S Klingler, KS Hsu, G Hua, ML Martin, M Adileh, T Baslan, Z Zhang, PB Paty, Z Fuks, AM Brown, R Kolesnick Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells JCI Insight, 2022;7(5):. 2022 [PMID: 35260534] (IHC, Mouse) IHC Mouse
J Heil, V Olsavszky, K Busch, K Klapproth, C de la Torr, C Sticht, K Sandorski, J Hoffmann, H Schönhaber, J Zierow, M Winkler, CD Schmid, T Staniczek, DE Daniels, J Frayne, G Metzgeroth, D Nowak, S Schneider, M Neumaier, V Weyer, C Groden, HJ Gröne, K Richter, C Mogler, MM Taketo, K Schledzews, C Géraud, S Goerdt, PS Koch Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation Nature Communications, 2021;12(1):6963. 2021 [PMID: 34845225] (Flow Cytometry, Mouse) Flow Cytometry Mouse
IC Young, B Wu, J Andricovic, ST Chuang, R Li, A Tzatsos, RC Wu, MY Wu Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling Cell Reports, 2021;37(8):110036. 2021 [PMID: 34818550] (Western Blot, Human) Western Blot Human
F Progatzky, M Shapiro, SH Chng, B Garcia-Cas, CH Classon, S Sevgi, A Laddach, AC Bon-Frauch, R Lasrado, M Rahim, EM Amaniti, S Boeing, K Shah, LJ Entwistle, A Suárez-Bon, MS Wilson, B Stockinger, V Pachnis Regulation of intestinal immunity and tissue repair by enteric glia Nature, 2021;599(7883):125-130. 2021 [PMID: 34671159] (IHC, Mouse) IHC Mouse
K Kimura, M Breitbach, FA Schildberg, M Hesse, BK Fleischman Bone marrow CD73+ mesenchymal stem cells display increased stemness in vitro and promote fracture healing in vivo Bone Reports, 2021;15(0):101133. 2021 [PMID: 34632004] (IHC, Mouse) IHC Mouse
T Aschacher, K Schmidt, O Aschacher, E Eichmair, U Baranyi, B Winkler, M Grabenwoeg, A Spittler, F Enzmann, B Messner, J Riebandt, G Laufer, M Bergmann, M Ehrlich Telocytes in the human ascending aorta: Characterization and exosome-related KLF-4/VEGF-A expression Journal of Cellular and Molecular Medicine, 2021;0(0):. 2021 [PMID: 34562312] (Flow Cytometry, Human) Flow Cytometry Human
K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis Journal of pharmacological sciences, 2021;146(3):125-135. 2021 [PMID: 34030795] (IHC, Rat) IHC Rat
W Jia, L Kong, H Kidoya, H Naito, F Muramatsu, Y Hayashi, HY Hsieh, D Yamakawa, DK Hsu, FT Liu, N Takakura Indispensable role of Galectin-3 in promoting quiescence of hematopoietic stem cells Nature Communications, 2021;12(1):2118. 2021 [PMID: 33837181] (IHC, Mouse) IHC Mouse
K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules Scientific Reports, 2021;11(1):1110. 2021 [PMID: 33441739] (IHC, Mouse) IHC Mouse
N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020;11(1):5171. 2020 [PMID: 33057002] (Western Blot, Mouse) Western Blot Mouse
Show All 46 Publications.

Reviews for CD117/c-kit Antibody (AF1356) (4) 4.34

Average Rating: 4.3
(Based on 4 reviews)
We have 4 reviews tested in 1 species: Mouse.

Reviews using AF1356:
Filter by Applications
WB
(2)
IF
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Mouse 03/23/2018
View

Summary

ApplicationWestern Blot
Sample Testedmouse es cells
SpeciesMouse
LotIEO0217011
  5
reviewed by:
Verified Customer
WB Mouse 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Comments

CommentsPublished See PMID 24375406
  4
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22262798
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22262798

Details

Detection NotesSee PMID 22262798

Comments

Comments"With 2nd Ab: AlexaFluor 546-conjugated donkey anti-goat IgG (Molecular probes A11056)"See PMID 22262798
  4
reviewed by:
Verified Customer
ICC Mouse 01/05/2015
View

Summary

ApplicationImmunocytochemistry
Sample TestedSee PMID 24375406
SpeciesMouse

Blocking

Blocking DetailsSee PMID 24375406

Details

Detection NotesSee PMID 24375406

Product General Protocols

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FAQs for CD117/c-kit Antibody (AF1356) (0)

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Secondary Antibodies

 

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Blogs on CD117/c-kit.

Do you see what I see? I c-Kit
The c-Kit (CD117) proto-oncogene is a 145 kD receptor tyrosine kinase family closely related to platelet-derived growth factor receptor (PDGFR). It is a transmembrane receptor and the cellular homolog of the HZ4-feline sarcoma virus transforming gene ...  Read full blog post.

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Recent Reviews

4.3
5
1
4
3
3
0
2
0
1
0

 
Verified Customer
03/23/2018
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: WB
Species: Mouse

 
Verified Customer
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol KIT
Uniprot