CD44 Antibody (2C5) [Unconjugated] - s Pan Specific

Images

 
Simple Western lane view shows lysates of human tonsil tissue, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 156 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human CD44 s Pan ...read more
Western blot shows lysates of PC‑3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) ...read more
CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, IHC, IP, CyTOF-ready
Clone
2C5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CD44 Antibody (2C5) [Unconjugated] - s Pan Specific Summary

Immunogen
Recombinant human CD44v3-10 (includes the invariant N-terminal exons and CD44v3-10 exons)
Specificity
Detects human CD44s on a panel of CD44 transfected COS cells by flow cytometry (Fox, S.B. et al. (1994) Cancer Res. 54:4539). This antibody recognizes an epitope in the invariant N-terminal region of all CD44 protein isoforms.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD44
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 20 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
  • CyTOF-ready
Control
CD44 Knockout HeLa Cell Lysate
Reviewed Applications
Read 5 Reviews rated 4.6
using
BBA10 in the following applications:

Publications
Read Publications using
BBA10 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD44 Antibody (2C5) [Unconjugated] - s Pan Specific

  • CD44 antigen
  • CD44 molecule (Indian blood group)
  • CD44
  • CD44R
  • CDw44
  • cell surface glycoprotein CD44
  • chondroitin sulfate proteoglycan 8
  • CSPG8
  • ECMR-III
  • epican
  • Extracellular matrix receptor III
  • GP90 lymphocyte homing/adhesion receptor
  • HCAM
  • HCELL
  • hematopoietic cell E- and L-selectin ligand
  • Heparan sulfate proteoglycan
  • Hermes antigen
  • homing function and Indian blood group system
  • HUTCH-I
  • Hyaluronate receptor
  • IN
  • LHR
  • MC56
  • MDU2
  • MDU2CD44 antigen (homing function and Indian blood group system)
  • MDU3
  • MDU3CDW44
  • MIC4
  • MIC4MGC10468
  • MUTCH-I
  • Pgp1
  • PGP-1
  • PGP-I
  • Phagocytic glycoprotein 1
  • Phagocytic glycoprotein I

Background

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44s, does not include the variable segments (1‑3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80-200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83%, and 79% identity with corresponding mouse, rat, equine, canine, and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
  3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
  4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
  7. Murakami, D. et al. (2003) Oncogene 22:1511.
  8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD44 Antibody (BBA10)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(4)
IHC
(2)
Immunoprecipitation
(3)
Western Blot
(10)
All Applications
Filter By Species
Human
(19)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using BBA10 Applications Species
SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration Mol Med Rep, 2019;0(0):. 2019 [PMID: 30720108] (Flow Cytometry, Rat) Flow Cytometry Rat
T Liu, L Jiang, O Tavana, W Gu The deubiquitylase OTUB1 mediates ferroptosis via stabilization of SLC7A11 Cancer Res., 2019;0(0):. 2019 [PMID: 30709928] (Western Blot, Human) Western Blot Human
T Murakami, J Kim, Y Li, GE Green, A Shikanov, A Ono Secondary lymphoid organ fibroblastic reticular cells mediate trans-infection of HIV-1 via CD44-hyaluronan interactions Nat Commun, 2018;9(1):2436. 2018 [PMID: 29934525] (Western Blot, Human) Western Blot Human
C Donnelly, B Dykstra, N Mondal, J Huang, BJ Kaskow, R Griffin, R Sackstein, C Baecher-Al Optimizing human Treg immunotherapy by Treg subset selection and E-selectin ligand expression Sci Rep, 2018;8(1):420. 2018 [PMID: 29323143] (Immunoprecipitation, Human) Immunoprecipitation Human
S Liu, C Cheng Akt signaling is sustained by a CD44 splice isoform-mediated positive feedback loop Cancer Res., 2017;0(0):. 2017 [PMID: 28533273] (Western Blot, Human) Western Blot Human
M Silva, RK Fung, CB Donnelly, PA Videira, R Sackstein Cell-Specific Variation in E-Selectin Ligand Expression among Human Peripheral Blood Mononuclear Cells: Implications for Immunosurveillance and Pathobiology J. Immunol, 2017;0(0):. 2017 [PMID: 28330896] (Immunoprecipitation, Human) Immunoprecipitation Human
Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas Stem Cell Reports, 2016;0(0):. 2016 [PMID: 27916539] (Western Blot, Human) Western Blot Human
Brad Dykstra Glycoengineering of E-selectin ligands by intracellular versus extracellular fucosylation differentially affects osteotropism of human mesenchymal stem cells Stem Cells, 2016;0(0):. 2016 [PMID: 27335219] (Immunoprecipitation, Human) Immunoprecipitation Human
Inada E, Saitoh I, Watanabe S, Aoki R, Miura H, Ohtsuka M, Murakami T, Sawami T, Yamasaki Y, Sato M PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells. Int J Oral Sci, 0;7(3):144-54. 0 [PMID: 26208039] (ICC, Human) ICC Human
AbuSamra D, Al-Kilani A, Hamdan S, Sakashita K, Gadhoum S, Merzaban J Quantitative Characterization of E-selectin Interaction with Native CD44 and P-selectin Glycoprotein Ligand-1 (PSGL-1) Using a Real Time Immunoprecipitation-based Binding Assay. J Biol Chem, 2015;290(35):21213-30. 2015 [PMID: 26124272] (Western Blot, Human) Western Blot Human
Show All 21 Publications.

Reviews for CD44 Antibody (BBA10) (5) 4.65

Average Rating: 4.6
(Based on 5 reviews)
We have 5 reviews tested in 1 species: Human.

Reviews using BBA10:
Filter by Applications
WB
(5)
All Applications
Filter by Species
Human
(5)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 01/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 21364036
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 21364036

Details

Detection NotesSee PMID: 21364036

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 21364036
 
reviewed by:
Anonymous
WB Human 01/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 21730156
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 21730156

Details

Detection NotesSee PMID: 21730156

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 21730156
 
reviewed by:
Anonymous
WB Human 01/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 23736004
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 23736004

Details

Detection NotesSee PMID: 23736004

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID: 23736004
 
reviewed by:
Anonymous
WB Human 01/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 23457460
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 23457460

Details

Detection NotesSee PMID: 23457460

Comments

CommentsIndependent review from antibodyresource.comClean and specific antibody picks up predominantly mature form of CD44 and other isoforms in concentrated samples. Published in PMID: 23457460.
 
reviewed by:
Anonymous
WB Human 01/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID: 24512624
SpeciesHuman

Blocking

Blocking DetailsSee PMID: 24512624

Details

Detection NotesSee PMID: 24512624

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID:24512624

Product General Protocols

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FAQs for CD44 Antibody (BBA10) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin FAB4948A
Phycoerythrin FAB4948P

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Recent Reviews

4.6
5
3
4
2
3
0
2
0
1
0

 
Anonymous
01/16/2015
Application: WB
Species: Human

 
Anonymous
01/16/2015
Application: WB
Species: Human

 
Anonymous
01/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol CD44
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