OCT4 Antibody [Alexa Fluor 647]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, Bovine, Feline, PorcineSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit

OCT4 Antibody [Alexa Fluor 647] Summary

Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein (between residues 100-200) [UniProt# Q01860]
Localization
Nuclear
Marker
Embryonic Stem Cell Marker
Predicted Species
Porcine (93%), Bovine (93%), Feline (93%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
POU5F1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 647
Format
Alexa Fluor 647 (A=650, E=668)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This OCT4 antibody is useful for Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot where a band is seen at ~40 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, monkey, rat, mouse. Immunogen has 93% homology to cow, feline and pig. Customer feedback suggests that this antibody does not give good results in IHC-P on feline tissues.

Notes

Alexa Flour (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research.

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Alternate Names for OCT4 Antibody [Alexa Fluor 647]

  • class 5, transcription factor 1
  • MGC22487
  • Oct-3
  • OCT3Oct4
  • Oct-4
  • Octamer-binding protein 3
  • OTF3POU domain class 5, transcription factor 1
  • OTF4
  • POU class 5 homeobox 1
  • POU-type homeodomain-containing DNA-binding protein

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OCT4 Antibody (NB100-2379AF647) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 700 Labeled NB100-2379AF700

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Blogs on OCT4.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Histones, Bmi1 & OCT4: Investigating the Secrets of ESC Pluripotency
Epigenetic alterations have come to prominence in biomedical research. In particular, hypermethylation of CpG islands located in the promoter regions of tumor-suppressor genes is now firmly established as an important mechanism for gene inactivation i...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

The Sox2 Antibody Aids Brain Cancer Research
The Sox2 antibody is widely used in sensory, neuroscience and stem cell marker research. Recently, Sox2 antibody preparations identified the Sox2 protein as a marker for malignant neural gliomas. We at Novus Biologicals offer a wide variety of highly...  Read full blog post.

Not as Pluripotent as You Used to Be: Embryonic Stem Cell Markers and the Aging Process
We at Novus Biologicals recently extended our antibody catalog to include several embryonic stem cells (ESC) antibodies validated for use in FACS (fluorescent activated cell sorting) assays. Among them was Oct4, which recently became the focus of an i...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol POU5F1

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