c-Abl Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF‑7 human breast cancer cell line, and MDA‑MB‑453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c‑Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

c-Abl Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human c-Abl
Ala941-Val1140
Accession # NP_009297
Specificity
Detects human c-Abl in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Control
c-Abl Lysate (NBP2-65256)
Publications
Read Publication using
AF5414 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for c-Abl Antibody [Unconjugated]

  • Abelson murine leukemia viral oncogene homolog 1
  • ABL
  • ABL1
  • bcr/abl
  • BCR-ABL1
  • c-abl oncogene 1, non-receptor tyrosine kinase
  • c-abl oncogene 1, receptor tyrosine kinase
  • cAbl
  • c-Abl
  • EC 2.7.10
  • EC 2.7.10.2
  • JTK7
  • JTK7bcr/abl
  • p150bcr/c-abl oncogene protein
  • Proto-oncogene c-Abl
  • proto-oncogene tyrosine-protein kinase ABL1
  • tyrosine-protein kinase ABL1
  • v-abl Abelson murine leukemia viral oncogene homolog 1
  • v-abl

Background

c-Abl (Abelson murine leukemia viral homolog 1) is a cytosolic member of the ABL subfamily, protein tyrosine kinase family of enzymes. It is ubiquitously expressed, and participates in multiple processes such as cell migration, actin reorganization, DNA damage response and apoptosis. Human c-Abl (I-B) is 1149 amino acids (aa) in length. It is myristoylated on Gly2 and contains one SH3 domain (aa 80‑140), an SH2 domain (aa 146‑236), a protein kinase region (aa 261‑512), three NLS's (aa 620‑634; 726‑739; 778‑791), one DNA-binding region (aa 888‑988), an NES motif (aa 1109‑1119) and an actin F-binding domain (aa 1120‑1149). There is one alternate splice form (I-A) that contains a 26 aa substitution for the N-terminal 45 amino acids. In chronic myelogenous leukemia, c-Abl is fused to the Bcr gene product, resulting in the production of a constitutively active tyrosine kinase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Abl Antibody (AF5414)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow.


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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ABL1
Entrez
Uniprot