CD20 Antibody (AISB12)

Images

 
Western Blot: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Immunoblot of Balb/c thymus (lane 1) and A20 (lane 2) cell lysates with Anti-Mouse CD20 Purified.
Flow Cytometry: MS4A1/CD20 Antibody (AISB12) [NBP1-43435] - Staining of BALB/c splenocytes with Anti-Mouse CD19 Alexa Fluor (R) 647 and 1.0 micrograms conjugated anti-Mouse CD20 Purified followed by Anti-Rat IgG PE. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow
Clone
AISB12
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD20 Antibody (AISB12) Summary

Immunogen
The immunogen for this antibody was CD20.
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MS4A1
Purity
Protein A or G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
Application Notes
This AISB12 antibody has been tested by immunoblot on A20 cell lysates and flow cytometry on mouse splenocytes.
Publications
Read Publications using NBP1-43435.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD20 Antibody (AISB12)

  • B1
  • B-lymphocyte antigen CD20
  • B-lymphocyte cell-surface antigen B1
  • B-lymphocyte surface antigen B1
  • Bp35
  • Bp35MGC3969
  • CD20 antigen
  • CD20 receptor
  • CD20
  • CD20S7
  • CVID5
  • LEU-16
  • Leukocyte surface antigen Leu-16
  • Ly-44
  • Membrane-spanning 4-domains subfamily A member 1
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A1
  • MS4A2
  • S7

Background

CD20 is a B cell-specific 32kDa membrane protein involved in intracellular calcium regulation. CD20 expression begins after the induction of CD19 during development, and is coexpressed with IgM during the pre-B to immature B cell transition in bone marrow. Expression then increases during maturation with almost all mature cells expressing some level of CD20. The human CD20 antigen has proven to be an effective immunotherapeutic target in B cell lymphoma and several autoimmune diseases including rheumatoid arthritis and type I diabetes. CD20 consists of 4 transmembrane domains and a 44 amino acid extracellular loop domain where AISB12 binds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD20 Antibody (NBP1-43435)(3)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD20 Antibody (NBP1-43435). (Showing 1 - 1 of 1 FAQs).

  1. Does this clone of anti-mouse CD20 mAb deplete mouse CD20+ B cells in vivo? If so, do you have references for it?
    • We have only tested NBP1-43435 in Western blot and Flow cytometry applications, and have not evaluated whether it can deplete mouse CD20+ cells in vivo.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43435AF405
Alexa Fluor 488 Labeled NBP1-43435AF488
Alexa Fluor 647 Labeled NBP1-43435AF647
Alexa Fluor 700 Labeled NBP1-43435AF700
Allophycocyanin Labeled NBP1-43435APC
DyLight 350 Labeled NBP1-43435UV
DyLight 405 Labeled NBP1-43435V
DyLight 488 Labeled NBP1-43435G
DyLight 680 Labeled NBP1-43435FR
DyLight 755 Labeled NBP1-43435IR
PE Labeled NBP1-43435PE
PerCP Labeled NBP1-43435PCP

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Bioinformatics Tool for CD20 Antibody (NBP1-43435)

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Research Areas for CD20 Antibody (NBP1-43435)

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Blogs on CD20.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

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Bioinformatics

Gene Symbol MS4A1
Entrez