Lactate Dehydrogenase A/LDHA Antibody

Images

 
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.
Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336]
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.
Simple Western: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, Bv, PoSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.25 mg/ml

Lactate Dehydrogenase A/LDHA Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
Localization
Cytoplasm.
Predicted Species
Porcine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5ug/ml
  • Simple Western 1:50
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at approx. 36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 25004202)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Control Peptide
Lactate Dehydrogenase A/LDHA Peptide (NBP1-48336PEP)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NBP1-48336 in the following applications:

Publications
Read Publications using
NBP1-48336 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Porcine.

We have publications tested in 3 applications: IHC-Fr, IHC-P, WB.


Filter By Application
IHC-Fr
(1)
IHC-P
(1)
WB
(10)
All Applications
Filter By Species
Human
(2)
Mouse
(7)
Bovine
(3)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP1-48336 Applications Species
Dang DS, Buhler JF, Thornton KJ et al. Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness Meat Sci. Jul 28 2020 [PMID: 32739757] (WB, Bovine) WB Bovine
Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE Jan 30 2020 [PMID: 31999787] (WB, Mouse) WB Mouse
Apaoblaza A, Gerrard SD, Matarneh SK et al. Muscle from grass- and grain-fed cattle differs energetically Meat Sci. Nov 6 2019 [PMID: 31734468] (WB, Bovine) WB Bovine
Choi YS, Song JE, Lee JE et al. Hyperpolarized [1-13C]lactate flux increased in the hippocampal region in diabetic mice Mol Brain Nov 1 2019 [PMID: 31675964] (WB, Mouse) WB Mouse
Boyle J, Patronas NJ, Smirniotopoulos J et al CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease. Endocrine. 2019 Jul [PMID: 31062234] (IHC-P, Human)

Details:
Citation using the Alexa Fluor 488 version of this antibody.
IHC-P Human
Hulley PA, Bishop T, Vernet A et al. Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2. J. Pathol. Apr 18 2017 [PMID: 28418093]
Takenouchi T, Tsukimoto M, Iwamaru Y et al. Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells. Immunol. Lett. 2015 Aug 12 [PMID: 26277554] (WB, Mouse)

Details:
Lactate Dehydrogenase A/LDHA Antibody was used for WB on microvesicle and exosome enriched /depleted fractions from LPS-primed MG6 cells (mouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene) after ATP stimulation (Fig. 3A).
WB Mouse
Kim J, Han J, Jang Y et al. High-capacity glycolytic and mitochondrial oxidative metabolisms mediate the growth ability of glioblastoma Int. J. Oncol. 2015 Jul 21 [PMID: 26202438]
Pastor-Soler NM, Sutton TA, Mang HE et al. Muc1 is protective during kidney ischemia-reperfusion injury Am J Physiol Renal Physiol. 2015 Apr 28 [PMID: 25925251] (WB, Mouse) WB Mouse
Adochite RC, Moshnikova A, Carlin SD et al. Targeting Breast Tumors with pH (Low) Insertion Peptides. Mol. Pharm. 2014 Jul 08 [PMID: 25004202] (IHC-Fr, Mouse)

Details:
Lactate Dehydrogenase A antibody used at 1:100 dilution for IHC-Fr application on 4% PFA fixed cryosections of FVB/N-Tg(MMTV-PyVT)634Mul/J transgenic female mice (develops palpable mammary tumors at 12-15 wk of age) as well as tissues from control noncarrier FVB/NJ female mice (Figures 6 and 7). IHC-Fr of LDHA also performed on the cryosections of tumors that were generated by grafting 4T1 mouse mammary tumor cell line on BALB/c mice.
IHC-Fr Mouse
Show All 15 Publications.

Reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NBP1-48336:
Filter by Applications
WB
(3)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
4
reviewed by:
Anonymous
WB Human 03/10/2020
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line
SpeciesHuman
Lotb-3

Comments

CommentsUsed it in 1:1000 with milk TBST.
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
5
reviewed by:
Eric England
WB Other 10/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at Room Temperature for 1 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
4
reviewed by:
Eric England
WB Other 10/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Immunohistochemistry Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
5
reviewed by:
Anony- mous
IHC Mouse 03/17/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
FileView PDF

Product General Protocols

View specific protocols for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-48336AF350
Alexa Fluor 405 NBP1-48336AF405
Alexa Fluor 488 NBP1-48336AF488
Alexa Fluor 532 NBP1-48336AF532
Alexa Fluor 594 NBP1-48336AF594
Alexa Fluor 647 NBP1-48336AF647
Alexa Fluor 700 NBP1-48336AF700
Alexa Fluor 750 NBP1-48336AF750
Biotin NBP1-48336B
DyLight 350 NBP1-48336UV
DyLight 405 NBP1-48336V
DyLight 488 NBP1-48336G
DyLight 550 NBP1-48336R
DyLight 594 NBP1-48336DL594
DyLight 650 NBP1-48336C
DyLight 680 NBP1-48336FR
DyLight 755 NBP1-48336IR
FITC NBP1-48336F
HRP NBP1-48336H
Janelia Fluor 549 NBP1-48336JF549
Janelia Fluor 646 NBP1-48336JF646

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Anonymous
03/10/2020
Application: WB
Species: Human

Eric England
10/10/2014
Application: WB
Species: Other

Eric England
10/01/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol LDHA
Entrez
Uniprot