Lactate Dehydrogenase A/LDHA Antibody - BSA Free

Images

 
Simple Western: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions ...read more
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Increased lactate level detected in the hippocampus of streptozotocin-injected mice fed an HFD (HFD + STZ).b LDHA and LDHB protein levels were measured ...read more
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.
Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336]
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells
Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - An intracellular stain was performed on HeLa cells with Lactate Dehydrogenase A/LDHA Antibody NBP1-48336AF488 (blue) and a matched isotype control ...read more
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Po, BvSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Lactate Dehydrogenase A/LDHA Antibody - BSA Free Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
Localization
Cytoplasm.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:50
  • Western Blot 0.5ug/ml
Application Notes
Use in ELISA reported in scientific literature (PMID:35052722).This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at approx. 36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 25004202)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue..
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
HeLa Whole Cell Lysate
Control Peptide
Lactate Dehydrogenase A/LDHA Peptide (NBP1-48336PEP)
Reviewed Applications
Read 4 Reviews rated 4.5
using
NBP1-48336 in the following applications:

Publications
Read Publications using
NBP1-48336 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody - BSA Free

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336)(26)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Porcine.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
WB
(13)
All Applications
Filter By Species
Human
(5)
Mouse
(8)
Bovine
(5)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NBP1-48336 Applications Species
Marwarha G, Rosand o, Scrimgeour N et al. miR-210 Regulates Apoptotic Cell Death during Cellular Hypoxia and Reoxygenation in a Diametrically Opposite Manner Biomedicines Dec 25 2021 [PMID: 35052722] (ELISA) ELISA
Shen W, Lee SR, Mathai AE et al. Effect of selectively knocking down key metabolic genes in MUller glia on photoreceptor health Glia Apr 9 2021 [PMID: 33835598]
Pinto IF, Soares RRG, MAkinen ME et al. Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production ACS sensors Mar 26 2021 [PMID: 33724791]
Osis G, Traylor AM, Black LM et al. Expression of Lactate Dehydrogenase A and B isoforms in the mouse kidney American journal of physiology. Renal physiology Mar 15 2021 [PMID: 33719570]
Weiss-Sadan, T, Itzhak, G Et al. Cathepsin L Regulates Metabolic Networks Controlling Rapid Cell Growth and Proliferation. Mol Cell Proteomics Jul 1 2019 [PMID: 33451519] (IHC, Human) IHC Human
Dang DS, Buhler JF, Thornton KJ et al. Myosin heavy chain isoform and metabolic profile differ in beef steaks varying in tenderness Meat Sci. Jul 28 2020 [PMID: 32739757] (WB, Bovine) WB Bovine
Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE Jan 30 2020 [PMID: 31999787] (WB, Mouse) WB Mouse
Knowles HJ Distinct roles for the hypoxia-inducible transcription factors HIF-1alpha and HIF-2alpha in human osteoclast formation and function Scientific reports Dec 3 2020 [PMID: 33273561] (WB, Human) WB Human
Duda P, Janczara J et al. The Reverse Warburg Effect is Associated with Fbp2-Dependent Hif1 alpha Regulation in Cancer Cells Stimulated by Fibroblasts. Cells Jan 14 2020 [PMID: 31947613] (ICC/IF, Mouse) ICC/IF Mouse
Show All 26 Publications.

Reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (4) 4.54

Average Rating: 4.5
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Other.

Reviews using NBP1-48336:
Filter by Applications
WB
(3)
IHC
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
4
reviewed by:
Verified Customer
WB Human 03/10/2020
View

Summary

ApplicationWestern Blot
Sample TestedHepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line
SpeciesHuman
Lotb-3

Comments

CommentsUsed it in 1:1000 with milk TBST.
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
5
reviewed by:
Eric England
WB Other 10/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at Room Temperature for 1 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
4
reviewed by:
Eric England
WB Other 10/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Immunohistochemistry Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
5
reviewed by:
Bryan Tinsley
IHC Mouse 03/17/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
FileView PDF

Product General Protocols

View specific protocols for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4.5
5
2
4
2
3
0
2
0
1
0

Verified Customer
03/10/2020
Application: WB
Species: Human

Eric England
10/10/2014
Application: WB
Species: Other

Eric England
10/01/2014
Application: WB
Species: Other

Bioinformatics

Gene Symbol LDHA
Entrez
Uniprot