Lactate Dehydrogenase A/LDHA Antibody

Images

 
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in porcine skeletal muscle lysate. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Immunocytochemical analysis of Lactate Dehydrogenase A in HeLa cells
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of postnatal day 8(P8) Plp-EGFP transgenic mice brain section. Image from verified customer review.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - IHC staining of Lactate Dehydrogenase A in lung tissue.
Simple Western: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Simple Western lane view shows a specific band for Lactate Hydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, BvSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.25 mg/ml

Lactate Dehydrogenase A/LDHA Antibody Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
Localization
Cytoplasm.
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at approx. 36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 25004202)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
HeLa Lysate (NB800-PC1)
Control Peptide
Lactate Dehydrogenase A/LDHA Peptide (NBP1-48336PEP)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP1-48336 in the following applications:

Publications
Read Publications using
NBP1-48336 in the following applications:

Reactivity Notes

Human and mouse. Predicted to react with bovine and porcine based on 100% sequence homology. Bovine data from customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.25 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Po, Bv
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-Fr, WB.


Filter By Application
IHC-Fr
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(2)
All Species
Showing Publications 1 - 4 of 4.
Publications using NBP1-48336 Applications Species
Hulley PA, Bishop T, Vernet A et al. Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2. J. Pathol. Apr 18 2017 [PMID: 28418093]
Takenouchi T, Tsukimoto M, Iwamaru Y et al. Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells. Immunol. Lett. 2015 Aug 12 [PMID: 26277554] (WB, Mouse)

Details:
Lactate Dehydrogenase A/LDHA Antibody was used for WB on microvesicle and exosome enriched /depleted fractions from LPS-primed MG6 cells (mouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene) after ATP stimulation (Fig. 3A).
WB Mouse
Kim J, Han J, Jang Y et al. High-capacity glycolytic and mitochondrial oxidative metabolisms mediate the growth ability of glioblastoma Int. J. Oncol. 2015 Jul 21 [PMID: 26202438]
Adochite RC, Moshnikova A, Carlin SD et al. Targeting Breast Tumors with pH (Low) Insertion Peptides. Mol. Pharm. 2014 Jul 08 [PMID: 25004202] (IHC-Fr, Mouse)

Details:
Lactate Dehydrogenase A antibody used at 1:100 dilution for IHC-Fr application on 4% PFA fixed cryosections of FVB/N-Tg(MMTV-PyVT)634Mul/J transgenic female mice (develops palpable mammary tumors at 12-15 wk of age) as well as tissues from control noncarrier FVB/NJ female mice (Figures 6 and 7). IHC-Fr of LDHA also performed on the cryosections of tumors that were generated by grafting 4T1 mouse mammary tumor cell line on BALB/c mice.
IHC-Fr Mouse

Reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Other.
We have 3 reviews tested in 2 application: WB, IHC.

Reviews using NBP1-48336:
Filter by Applications
WB
(2)
IHC
(1)
All Applications
Filter by Species
Mouse
(1)
Other
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
reviewed by:
Eric England
WB Other 10/10/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20 for 1 hr

Primary Anitbody

Dilution Ratio1/2000 at Room Temperature for 1 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Western Blot Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
reviewed by:
Eric England
WB Other 10/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole Skeletal Muscle Lysate
SpeciesOther
LotA

Blocking

Blocking Details3% Casein in PBS with 0.1% Tween-20

Primary Anitbody

Dilution Ratio1/2000 at 4C for 18 hr

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IRDye 800CW - Licor Biosciences
Secondary Manufacturer Cat#926-32213
Secondary Concentration1/25,000

Details

Detection NotesLicor Odyssey
Immunohistochemistry Lactate Dehydrogenase A/LDHA NBP1-48336
Enlarge
reviewed by:
Anony- mous
IHC Mouse 03/17/2014
View

Summary

ApplicationImmunohistochemistry
SpeciesMouse
FileView PDF

Product General Protocols

View specific protocols for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-48336AF405
Alexa Fluor 488 Labeled NBP1-48336AF488
Alexa Fluor 647 Labeled NBP1-48336AF647
Alexa Fluor 700 Labeled NBP1-48336AF700
Biotin Labeled NBP1-48336B
DyLight 405 Labeled NBP1-48336V
DyLight 488 Labeled NBP1-48336G
DyLight 550 Labeled NBP1-48336R
DyLight 650 Labeled NBP1-48336C
FITC Labeled NBP1-48336F
HRP Labeled NBP1-48336H

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Eric England
10/10/2014
Application: WB
Species: Other

Eric England
10/01/2014
Application: WB
Species: Other

Anony- mous
03/17/2014
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol LDHA
Entrez
Uniprot