Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in HeLa whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Analysis of Lactate Dehydrogenase A in NIH/3T3 whole cell lysates.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lactate Dehydrogenase A expression in bovine skeletal muscle lysate. Image from verified customer review.
Immunohistochemistry: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Staining of Lactate Dehydrogenase A in lung tissue.
Simple Western: Lactate Dehydrogenase A/LDHA Antibody [NBP1-48336] - Lane view shows a specific band for Lactate dehydrogenase A in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions ...read more
This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at approx. 36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 25004202)
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Porcine reactivity reported in scientific literature (Elgin et al).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for Lactate Dehydrogenase A/LDHA Antibody
Cell proliferation-inducing gene 19 protein
Lactate Dehydrogenase A
lactate dehydrogenase M
LDH muscle subunit
L-lactate dehydrogenase A chain
proliferation-inducing gene 19
Renal carcinoma antigen NY-REN-59
Lactate dehydrogenase (LDH) is a ubiquitous enzyme that catalyzes the inter-conversion of pyruvate and lactate, with simultaneous inter-conversion of NADH and NAD+. Mammalian LDH has five tetrameric isozymes comprising combinations of two subunits (i) A subunit (LDHA or LDHM) predominates in skeletal muscle where it involve in anaerobic metabolism and pyruvate reduction (ii) B subunit (LDHB) predominates in cardiac muscle where it helps catalyze aerobic oxidation of pyruvate. Several human cancers such as including renal, breast, gastric, nasopharyngeal cancer etc. have higher LDHA levels compared to normal tissues and LDHA upregulation ensures efficient anaerobic/glycolytic metabolism for tumor cells with reduced oxygen dependency. Moreover, LDHA also plays an important role in the development, invasion and metastasis of several malignancies and induced suppression of LDHA in cancer cells has been shown to trigger ROS burst, mitochondrial pathway apoptosis and limited tumorigenic potential. Defects in LDHA have also been linked to exertional myoglobinuria and glycogen storage disease type 11 (GSD11).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.