ME2 Antibody

Images

 
Independent Antibodies: Western Blot: ME2 Antibody [NBP1-89559] - Analysis using Anti-ME2 antibody NBP1-89559 (A) shows similar pattern to independent antibody NBP1-82434 (B).
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human colon.
Western Blot: ME2 Antibody [NBP1-89559] - Analysis in human cell line Daudi.
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human duodenum shows high expression.
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human liver shows low expression as expected.
Orthogonal Strategies: Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining in human duodenum and liver tissues using anti-ME2 antibody. Corresponding ME2 RNA-seq data are presented for the same ...read more
Independent Antibodies: Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-ME2 antibody NBP1-89559 (A) shows similar protein ...read more
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human lymph node.
Immunohistochemistry-Paraffin: ME2 Antibody [NBP1-89559] - Staining of human cerebral cortex.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ME2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KVISKPISEHKILFLGAGEAALGIANLIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPESIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSNPTA
Specificity
Specificity of human ME2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ME2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
ME2 Protein (NBP1-89559PEP)
Publications
Read Publications using NBP1-89559.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for ME2 Antibody

  • EC 1.1.1
  • EC 1.1.1.38
  • malate dehydrogenase
  • Malic enzyme 2
  • malic enzyme 2, NAD(+)-dependent, mitochondrial
  • NAD-dependent malic enzyme, mitochondrial
  • NAD-ME
  • ODS1
  • pyruvic-malic carboxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ME2 Antibody (NBP1-89559) (0)

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Secondary Antibodies

 

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Other Available Formats

Additional ME2 Products

Array NBP1-89559

Bioinformatics Tool for ME2 Antibody (NBP1-89559)

Discover related pathways, diseases and genes to ME2 Antibody (NBP1-89559). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on ME2

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Bioinformatics

Gene Symbol ME2
Entrez
Uniprot