Lactate Dehydrogenase A/LDHA Antibody [DyLight 488]

Images

 
There are currently no images for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

Lactate Dehydrogenase A/LDHA Antibody [DyLight 488] Summary

Immunogen
Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
Localization
Cytoplasm.
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Lactate Dehydrogenase A antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~36 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human and mouse. Predicted to react with bovine and porcine based on 100% sequence homology. Bovine data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody [DyLight 488]

  • Cell proliferation-inducing gene 19 protein
  • EC 1.1.1
  • EC 1.1.1.27
  • GSD11
  • Lactate Dehydrogenase A
  • lactate dehydrogenase M
  • LDH muscle subunit
  • LDH1
  • LDHA
  • LDH-A
  • LDHM
  • LDH-M
  • L-lactate dehydrogenase A chain
  • proliferation-inducing gene 19
  • Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-53421
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-92065
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NBP1-89843
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-39113
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-02541
Species: Hu, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP
NBP1-32398
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-34980
Species: Hu
NBP1-54755
Species: Hu
Applications: WB
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NBP1-59656
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-32259
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-85632
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-48336G
Species: Hu, Mu, Po, Bv
Applications: WB, ICC/IF, IHC-Fr, IHC-P

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G) (0)

There are no publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G) (0)

There are no reviews for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Lactate Dehydrogenase A/LDHA Products

Bioinformatics Tool for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G)

Discover related pathways, diseases and genes to Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G)

Discover more about diseases related to Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G).
 

Pathways for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G)

View related products by pathway.

PTMs for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G)

Learn more about PTMs related to Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G).
 

Research Areas for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336G)

Find related products by research area.

Blogs on Lactate Dehydrogenase A/LDHA

There are no specific blogs for Lactate Dehydrogenase A/LDHA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lactate Dehydrogenase A/LDHA Antibody [DyLight 488] and receive a gift card or discount.

Bioinformatics

Gene Symbol LDHA
Entrez