Lactate Dehydrogenase B Antibody

Images

 
Genetic Strategies: Knockout Validated: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF ...read more
Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Total protein from human HeLa and A431 cells, mouse MEF cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and ...read more
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. ...read more
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody [NBP2-53421] - Analysis of a FFPE tissue section of mouse liver using Lactate Dehydrogenase B antibody at 1:400 dilution. The staining was developed using ...read more
Flow Cytometry: Lactate Dehydrogenase B Antibody [NBP2-53421] - An intracellular stain was performed on SK-MEL-28 cells with Lactate Dehyrdrogenase B Antibody NBP2-53421F (blue) and a matched isotype control (orange). ...read more
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Lactate Dehydrogenase B Antibody Summary

Immunogen
Partial recombinant human Lactate Dehydrogenase B protein (amino acids 1-93) [P07195]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Knockout Validated
Control
Lactate Dehydrogenase B Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase B Antibody

  • EC 1.1.1
  • EC 1.1.1.27
  • Epididymis Secretory Protein Li 281
  • HEL-S-281
  • Lactate Dehydrogenase B
  • LDH heart subunit
  • LDHB
  • LDH-B
  • LDHBD
  • LDH-H
  • L-lactate dehydrogenase B chain
  • Renal Carcinoma Antigen NY-REN-46
  • TRG-5

Background

LDHB, also known as L-lactate dehydrogenase B chain, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein, fused to His-tagging at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase B Antibody (NBP2-53421) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-53421AF350
Alexa Fluor 405 NBP2-53421AF405
Alexa Fluor 488 NBP2-53421AF488
Alexa Fluor 532 NBP2-53421AF532
Alexa Fluor 594 NBP2-53421AF594
Alexa Fluor 647 NBP2-53421AF647
Alexa Fluor 700 NBP2-53421AF700
Alexa Fluor 750 NBP2-53421AF750
Biotin NBP2-53421B
DyLight 350 NBP2-53421UV
DyLight 405 NBP2-53421V
DyLight 488 NBP2-53421G
DyLight 550 NBP2-53421R
DyLight 594 NBP2-53421DL594
DyLight 650 NBP2-53421C
DyLight 680 NBP2-53421FR
DyLight 755 NBP2-53421IR
FITC NBP2-53421F
HRP NBP2-53421H
Janelia Fluor 549 NBP2-53421JF549
Janelia Fluor 646 NBP2-53421JF646

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Bioinformatics

Gene Symbol LDHB