Lactate Dehydrogenase C Antibody

Images

 
Western Blot: Lactate Dehydrogenase C Antibody [NBP1-92065] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining of human cell line A-431 shows positivity in plasma membrane.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining of human prostate shows low expression as expected.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase C Antibody [NBP1-92065] - Staining in human testis and prostate tissues using anti-LDHC antibody. Corresponding LDHC RNA-seq data are presented for the same tissues.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Lactate Dehydrogenase C Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MSTVKEQLIEKLIEDDENSQCKITIVGTGAVG
Specificity
Specificity of human Lactate Dehydrogenase C antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDHC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Lactate Dehydrogenase C Protein (NBP1-92065PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-92065 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Lactate Dehydrogenase C Antibody

  • Cancer/testis antigen 32
  • CT32EC 1.1.1.27
  • EC 1.1.1
  • lactate dehydrogenase C
  • lactate dehydrogenase C4
  • LDH testis subunit
  • LDH3MGC111073
  • LDH-C
  • LDHX
  • LDH-X
  • L-lactate dehydrogenase C chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48336
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-53421
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KO
NBP1-84310
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01506
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-45978
Species: Hu
Applications: WB, Flow, IHC
NBP1-87069
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, KD
NBP1-89134
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89133
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB110-3638
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, RI
NB100-410
Species: Hu, Mu
Applications: WB, IP
NBP1-89131
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-32263
Species: Hu
Applications: WB, IHC, IP
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-38770
Species: Hu, Mu
Applications: WB, IHC, IHC-P

Publications for Lactate Dehydrogenase C Antibody (NBP1-92065) (0)

There are no publications for Lactate Dehydrogenase C Antibody (NBP1-92065).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for Lactate Dehydrogenase C Antibody (NBP1-92065) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-92065:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Lactate Dehydrogenase C NBP1-92065
Enlarge
reviewed by:
Corey.
ICC Human 04/17/2013
View

Summary

ApplicationImmunocytochemistry
Sample TestedA549 human alveolar adenocarcinoma cell line
SpeciesHuman
LotA61501
CommentsThe antibody worked as expected.

Blocking

Blocking Details10% goat serum in PBS 1 hour

Primary Anitbody

Dilution Ratio1:250, 1:500 and 1:800 in 1 hour room temperature

Secondary Antibody

Secondary DescriptionInvitrogen A1100, Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) 1:500 in 2% goat serum PBS

Details

Detection NotesCaptured images using ZEISS FLUORESCENCE MICROSCOPE imager Z1
Fixation DetailsSeeded 150,000 A549 cells p106, on a cover slip in a 6 well plate with 2ml media the day before, 0.01% Triton x-100
Wash DescriptionPBS 3 times

Comments

CommentsThe antibody worked as expected.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lactate Dehydrogenase C Antibody (NBP1-92065). (Showing 1 - 1 of 1 FAQ).

  1. What isotype is this antibody?
    • NBP1-92065 is an IgG isotype antibody.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for Lactate Dehydrogenase C Antibody (NBP1-92065)

Discover related pathways, diseases and genes to Lactate Dehydrogenase C Antibody (NBP1-92065). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lactate Dehydrogenase C Antibody (NBP1-92065)

Discover more about diseases related to Lactate Dehydrogenase C Antibody (NBP1-92065).
 

Pathways for Lactate Dehydrogenase C Antibody (NBP1-92065)

View related products by pathway.

PTMs for Lactate Dehydrogenase C Antibody (NBP1-92065)

Learn more about PTMs related to Lactate Dehydrogenase C Antibody (NBP1-92065).
 

Research Areas for Lactate Dehydrogenase C Antibody (NBP1-92065)

Find related products by research area.

Blogs on Lactate Dehydrogenase C

There are no specific blogs for Lactate Dehydrogenase C, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

3
5
0
4
0
3
1
2
0
1
0

Corey.
04/17/2013
Application: ICC
Species: Human

Bioinformatics

Gene Symbol LDHC