MDH2 Antibody

Images

 
Western Blot: MDH2 Antibody [NBP1-32259] - A. 50 ug rat lung lysate/extract 10 % SDS-PAGEMDH2 antibody dilution: 1:5000
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded mouse heart. MDH2 antibody diluted at 1:500.
Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of MDH2 in HeLa whole cell lysate. Image courtesy of anonymous customer product review.
Western Blot: MDH2 Antibody [NBP1-32259] - Analysis of A293T whole cell lysate.
Western Blot: MDH2 Antibody [NBP1-32259] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000
Western Blot: MDH2 Antibody [NBP1-32259] - Sample(30 ug of whole cell lysate) A:293T 12% SDS PAGE diluted at 1:1000.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded OVCA xenograft.
Immunohistochemistry-Paraffin: MDH2 Antibody [NBP1-32259] - Paraffin-embedded mouse kidney. MDH2 antibody diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.76 mg/ml

Order Details

MDH2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MDH2. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MDH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32259 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.76 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MDH2 Antibody

  • EC 1.1.1
  • EC 1.1.1.37
  • malate dehydrogenase 2, NAD (mitochondrial)
  • MDH
  • MGC:3559
  • mitochondrial
  • MOR1

Background

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-32259
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Applications: WB, IHC, IHC-P

Publications for MDH2 Antibody (NBP1-32259) (0)

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Review for MDH2 Antibody (NBP1-32259) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32259:
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Images Ratings Applications Species Date Details
Western Blot MDH2 NBP1-32259
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reviewed by:
Hongxia Hu
WB Human 01/09/2011
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 10ug
SpeciesHuman
CommentsBand at expected size with one unspecific band

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 5 minutes, Specific Bands: 38 kDa, Non-Specific Bands: 65 kDa

Comments

CommentsBand at expected size with one unspecific band

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MDH2 Antibody (NBP1-32259) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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5
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4
1
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2
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1
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Hongxia Hu
01/09/2011
Application: WB
Species: Human

Bioinformatics

Gene Symbol MDH2