MCT1/SLC16A1 Antibody - BSA Free

Knockdown Validated: MCT1/SLC16A1 Antibody [NBP1-59656] - Western blotting to show knockdown of the Mct1 gene in BAT injected with Mct1 shRNA. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25265-3) licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-MCT1/SLC16A1 Antibody NBP1-59656 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Blockade of MCT1 inhibits the effects of optogenetic stimulation of sympathetic efferent fibers of BAT. (a) Cropped images of western blotting showing expression of MCT1 in the mitochondrial fractions (n = 2 mice). Full-length blots are presented in Supplementary Figure 2. VDAC: voltage-dependent anion channel, Cyt. C: cytochrome C, Cadhe: Cadherin. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-25265-3) licensed under a CC-BY licence.

Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining observed in the submucosa of mouse intestine.

Immunocytochemistry/Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). General cytoplasmic and membrane staining was observed.

Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.

• Reactivity: Hu, Mu, Pm
• Applications: WB, ICC/IF, IHC, IHC-P, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MCT1/SLC16A1 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal portion of the human Monocarboxylic acid transporter 1 protein (between residues 200-300) [UniProt P53985]
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC16A1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Western Blot 2.5 ug/mL
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  293 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for MCT1/SLC16A1 Antibody - BSA Free

• FLJ36745
• HHF7
• MCT 1
• MCT
• MCT1
• MCT1MGC44475
• member 1
• monocarboxylate transporter 1
• SLC16A1
• Solute carrier family 16 member 1
• solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Background

The protein encoded by this gene (Monocarboxylic acid transporter 1 / MCT1) is a proton-linked monocarboxylate transporter that catalyzes the movement of any monocarboxylates, such as branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate cross plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for MCT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCT1/SLC16A1 Antibody (NBP1-59656)(12)

Publications using NBP1-59656

• Tice AL, Laudato JA, Fadool DA et al. -Acute binge alcohol alters whole-body metabolism and the time-dependent expression of skeletal muscle specific metabolic markers for multiple days in mice American journal of physiology. Endocrinology and metabolism 2022-07-06 [PMID: 35793479] (WB, Mouse)
• Deguchi H, Yamashita T, Hiramoto N et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy Scientific reports 2022-04-15 [PMID: 35428816] (ICC/IF, Human)
• Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594]
  
  Details:
  Citation using the PE version of this antibody.
• Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites 2021-08-06 [PMID: 34436459] (WB, Human)
• Torres-Torrelo H, Ortega-Saenz P, Gao L, Lopez-Barneo J Lactate sensing mechanisms in arterial chemoreceptor cells Nature communications 2021-07-06 [PMID: 34230483] (IHC)
• Choi YS, Lee J, Lee HS et al. Offset of apparent hyperpolarized 13C lactate flux by the use of adjuvant metformin in ionizing radiation therapy in vivo NMR Biolmedical 2021-01-19 [PMID: 34080736] (WB, Mouse)
• Mostafa, D, Yanagiya, A Et al. Loss of beta -cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation. Commun Biol 2020-08-28 [PMID: 32859966] (WB, Mouse)
• Komoll RM, Hu Q, von Dohlen L et al. MicroRNA-342-3p is a regulator of hepatocellular carcinoma regression. J Hepatol 2020-08-02 [PMID: 32738449]
• Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE 2020-01-30 [PMID: 31999787] (WB, Mouse)
• Shtriker MG, Peri I, Taieb E et al. Galactomannan More Than Pectin Exacerbates Liver Injury In Mice Fed High-Fat, High-Cholesterol Diet. Mol Nutr Food Res 2018-07-27 [PMID: 30051965] (WB, Mouse)
• Kumar Dhruv, New Jacob, Vishwakarma Vikalp et al. Cancer-Associated Fibroblasts Drive Glycolysis in a Targetable Signaling Loop Implicated in Head and Neck Squamous Cell Carcinoma Progression. Cancer Research 2018-05-16 [PMID: 29769197] (ICC/IF, Human)
• Jeong JH, Chang JS, Jo YH. Intracellular glycolysis in brown adipose tissue is essential for optogenetically induced nonshivering thermogenesis in mice Sci Rep 2018-04-27 [PMID: 29704006] (WB, Mouse)

Bioinformatics

• Gene Symbol: SLC16A1
• Entrez:
  • 6566(Human)
• Uniprot:
  • P53985()