MCT1/SLC16A1 Antibody - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: MCT1/SLC16A1 Antibody [NBP1-59656] - Western blotting to show knockdown of the Mct1 gene in BAT injected with Mct1 shRNA. Image collected and cropped by CiteAb from the ...read more
Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Blockade of MCT1 inhibits the effects of optogenetic stimulation of sympathetic efferent fibers of BAT. (a) Cropped images of western blotting showing expression of ...read more
Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining ...read more
Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained ...read more
Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

MCT1/SLC16A1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human Monocarboxylic acid transporter 1 protein (between residues 200-300) [UniProt P53985]
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Western Blot 2.5 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using
NBP1-59656 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • WB
    7 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for MCT1/SLC16A1 Antibody - BSA Free

  • FLJ36745
  • HHF7
  • MCT 1
  • MCT
  • MCT1
  • MCT1MGC44475
  • member 1
  • monocarboxylate transporter 1
  • SLC16A1
  • Solute carrier family 16 member 1
  • solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Background

The protein encoded by this gene (Monocarboxylic acid transporter 1 / MCT1) is a proton-linked monocarboxylate transporter that catalyzes the movement of any monocarboxylates, such as branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate cross plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for MCT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13315
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81251
Species: Hu, Mu
Applications: IB, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP3-12238
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
NB300-877
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
NB100-64848
Species: Rt
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB110-39113
Species: Hu, Mu, Rb, Rt
Applications: ChIP, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-48615
Species: Hu
Applications: IHC, IHC-P, WB
D6050
Species: Hu
Applications: ELISA
NB120-14817
Species: Hu, Rt
Applications: ICC/IF, IHC, S-ELISA, WB
NBP1-59885
Species: Hu, Mu
Applications: IHC, IHC-P, WB
NBP2-57308
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
MAB1455
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
NBP2-43648
Species: Hu, Mu, Rt, Ze
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NB300-500
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
AF009
Species: Hu
Applications: IHC, WB
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP1-59656
Species: Hu, Mu, Pm
Applications: WB, ICC/IF, IHC, IHC-P, KD

Publications for MCT1/SLC16A1 Antibody (NBP1-59656)(11)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(2)
IHC
(1)
WB
(7)
All Applications
Filter By Species
Human
(4)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-59656 Applications Species
Deguchi H, Yamashita T, Hiramoto N et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy Scientific reports Apr 15 2022 [PMID: 35428816] (ICC/IF, Human) ICC/IF Human
Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites Aug 6 2021 [PMID: 34436459] (WB, Human) WB Human
Torres-Torrelo H, Ortega-Saenz P, Gao L, Lopez-Barneo J Lactate sensing mechanisms in arterial chemoreceptor cells Nature communications Jul 6 2021 [PMID: 34230483] (IHC) IHC
Jo YH Hydrocarboxylic acid receptor 1 in BAT regulates glucose uptake in mice fed a high-fat diet PLoS ONE Jan 30 2020 [PMID: 31999787] (WB, Mouse) WB Mouse
Shtriker MG, Peri I, Taieb E et al. Galactomannan More Than Pectin Exacerbates Liver Injury In Mice Fed High-Fat, High-Cholesterol Diet. Mol Nutr Food Res Jul 27 2018 [PMID: 30051965] (WB, Mouse) WB Mouse
Kumar Dhruv, New Jacob, Vishwakarma Vikalp et al. Cancer-Associated Fibroblasts Drive Glycolysis in a Targetable Signaling Loop Implicated in Head and Neck Squamous Cell Carcinoma Progression. Cancer Research 2018 May 16 [PMID: 29769197] (ICC/IF, Human) ICC/IF Human
Jeong JH, Chang JS, Jo YH. Intracellular glycolysis in brown adipose tissue is essential for optogenetically induced nonshivering thermogenesis in mice Sci Rep Apr 27 2018 [PMID: 29704006] (WB, Mouse) WB Mouse
Komoll RM, Hu Q, von Dohlen L et al. MicroRNA-342-3p is a regulator of hepatocellular carcinoma regression. journal of hepatology Jan 1 2020 (WB, Human) WB Human
Kumagai S, Koyama S, Itahashi K et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer cell Jan 25 2022 [PMID: 35090594]
Choi YS, Lee J, Lee HS et al. Offset of apparent hyperpolarized 13C lactate flux by the use of adjuvant metformin in ionizing radiation therapy in vivo NMR Biolmedical Jan 19 2021 [PMID: 34080736] (WB, Mouse) WB Mouse
Show All 11 Publications.

Reviews for MCT1/SLC16A1 Antibody (NBP1-59656) (0)

There are no reviews for MCT1/SLC16A1 Antibody (NBP1-59656). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MCT1/SLC16A1 Antibody (NBP1-59656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MCT1/SLC16A1 Products

Bioinformatics Tool for MCT1/SLC16A1 Antibody (NBP1-59656)

Discover related pathways, diseases and genes to MCT1/SLC16A1 Antibody (NBP1-59656). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MCT1/SLC16A1 Antibody (NBP1-59656)

Discover more about diseases related to MCT1/SLC16A1 Antibody (NBP1-59656).
 

Pathways for MCT1/SLC16A1 Antibody (NBP1-59656)

View related products by pathway.

PTMs for MCT1/SLC16A1 Antibody (NBP1-59656)

Learn more about PTMs related to MCT1/SLC16A1 Antibody (NBP1-59656).
 

Research Areas for MCT1/SLC16A1 Antibody (NBP1-59656)

Find related products by research area.

Blogs on MCT1/SLC16A1.

Monocarboxylate Transporter 1 (MCT1) - a novel oncogene
MCT1 is a proton-linked transport carrier that catalyzes the movement of short chain monocarboxylates (branched-chain oxo acids derived from leucine, valine, and isoleucine) across both plasma and inner mitochondrial membranes. In particular, subst...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MCT1/SLC16A1 Antibody - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol SLC16A1
Entrez
Uniprot