MCT1/SLC16A1 Antibody - BSA Free

Images

 
Genetic Strategies: Knockdown Validated: MCT1/SLC16A1 Antibody [NBP1-59656] - Western blotting to show knockdown of the Mct1 gene in BAT injected with Mct1 shRNA. Image collected and cropped by CiteAb from the ...read more
Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Ntera2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Blockade of MCT1 inhibits the effects of optogenetic stimulation of sympathetic efferent fibers of BAT. (a) Cropped images of western blotting showing expression of ...read more
Immunohistochemistry-Paraffin: MCT1/SLC16A1 Antibody [NBP1-59656] - Staining with Monocarboxylic acid transporter 1 antibody. Strong staining of lumen and crypt cells was observed with weaker cytoplasmic staining ...read more
Immunocytochemistry/ Immunofluorescence: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained ...read more
Western Blot: MCT1/SLC16A1 Antibody [NBP1-59656] - Monocarboxylic acid transporter 1 Antibody [NBP1-59656] - Jurkat cell lysate, concentration 2.5 ug/mL.
CNOT3 is essential for beta-cell maturation and identity. g) Immunoblot analysis of MCT1 and LDHA in islet lysates from 8-week-old control and Cnot3betaKO mice. This blot is a representative of three different blots. h ...read more
Localization of PGAM with antibody directed to the C-terminal peptide of the protein in nuclei of KLN-205 cells treated with drugs disturbing ribosomal biogenesisActD – actinomycin D; PI – propidium iodide. Bar=5 ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

MCT1/SLC16A1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal portion of the human Monocarboxylic acid transporter 1 protein (between residues 200-300) [UniProt P53985]
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC16A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Knockdown Validated
  • Western Blot 2.5 ug/mL
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
293 Whole Cell Lysate
HeLa Whole Cell Lysate
Publications
Read Publications using
NBP1-59656 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MCT1/SLC16A1 Antibody - BSA Free

  • FLJ36745
  • HHF7
  • MCT 1
  • MCT
  • MCT1
  • MCT1MGC44475
  • member 1
  • monocarboxylate transporter 1
  • SLC16A1
  • Solute carrier family 16 member 1
  • solute carrier family 16, member 1 (monocarboxylic acid transporter 1)

Background

The protein encoded by this gene (Monocarboxylic acid transporter 1 / MCT1) is a proton-linked monocarboxylate transporter that catalyzes the movement of any monocarboxylates, such as branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate cross plasma membrane. Mutations in this gene are associated with erythrocyte lactate transporter defect. Alternatively spliced transcript variants have been found for MCT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MCT1/SLC16A1 Antibody (NBP1-59656)(14)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.


Filter By Application
ICC/IF
(3)
IF/IHC
(1)
WB
(7)
All Applications
Filter By Species
Human
(3)
Mouse
(6)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using NBP1-59656 Applications Species
Chaumeil M, Guglielmetti C, Qiao K et al. Hyperpolarized (13)C metabolic imaging detects long-lasting metabolic alterations following mild repetitive traumatic brain injury Research Square 2023-08-15 [PMID: 37645937]
Hiltz RL, McCurdy DE, Moreland S et al. Effects of weaning on regulators of volatile fatty acid absorption and intracellular pH in Holstein calves JDS Communications 2021-11-01 [PMID: 36337096] (ICC/IF) ICC/IF
Tice AL, Laudato JA, Fadool DA et al. -Acute binge alcohol alters whole-body metabolism and the time-dependent expression of skeletal muscle specific metabolic markers for multiple days in mice American journal of physiology. Endocrinology and metabolism 2022-07-06 [PMID: 35793479] (WB, Mouse) WB Mouse
Deguchi H, Yamashita T, Hiramoto N et al. Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy Scientific reports 2022-04-15 [PMID: 35428816] (ICC/IF, Human) ICC/IF Human
Kumagai S, Koyama S, Itahashi K Et al. Lactic acid promotes PD-1 expression in regulatory T cells in highly glycolytic tumor microenvironments Cancer Cell 2022-01-29 [PMID: 35090594]

Details:
Citation using the PE version of this antibody.
Lai YC, Hsieh CY, Lu KY Et al. Monitoring Early Glycolytic Flux Alterations Following Radiotherapy in Cancer and Immune Cells: Hyperpolarized Carbon-13 Magnetic Resonance Imaging Study Metabolites 2021-08-06 [PMID: 34436459] (WB, Human) WB Human
Torres-Torrelo H, Ortega-Saenz P, Gao L, Lopez-Barneo J Lactate sensing mechanisms in arterial chemoreceptor cells Nature communications 2021-07-06 [PMID: 34230483] (IF/IHC) IF/IHC
Choi YS, Lee J, Lee HS et al. Offset of apparent hyperpolarized 13C lactate flux by the use of adjuvant metformin in ionizing radiation therapy in vivo NMR Biolmedical 2021-01-19 [PMID: 34080736] (WB, Mouse) WB Mouse
Mostafa, D, Yanagiya, A Et al. Loss of beta -cell identity and diabetic phenotype in mice caused by disruption of CNOT3-dependent mRNA deadenylation. Commun Biol 2020-08-28 [PMID: 32859966] (WB, Mouse) WB Mouse
Komoll RM, Hu Q, von Dohlen L et al. MicroRNA-342-3p is a regulator of hepatocellular carcinoma regression. J Hepatol 2020-08-02 [PMID: 32738449]
Show All 14 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MCT1/SLC16A1 Antibody (NBP1-59656) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional MCT1/SLC16A1 Products

Research Areas for MCT1/SLC16A1 Antibody (NBP1-59656)

Find related products by research area.

Blogs on MCT1/SLC16A1.

Monocarboxylate Transporter 1 (MCT1) - a novel oncogene
MCT1 is a proton-linked transport carrier that catalyzes the movement of short chain monocarboxylates (branched-chain oxo acids derived from leucine, valine, and isoleucine) across both plasma and inner mitochondrial membranes. In particular, subst...  Read full blog post.

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Bioinformatics

Gene Symbol SLC16A1
Entrez
Uniprot