ME1 Antibody

Images

 
Western Blot: ME1 Antibody [NBP1-32398] - Sample(30 ug whole cell lysate)A: A431 B: HeLa S3 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: ME1 Antibody [NBP1-32398] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: ME1 Antibody [NBP1-32398] - Paraffin-embedded Cal27 xenograft, using antibody at 1:500 dilution.
Western Blot: ME1 Antibody [NBP1-32398] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Western Blot: ME1 Antibody [NBP1-32398] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ME1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.
Localization
Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ME1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ME1 Antibody

  • EC 1.1.1
  • EC 1.1.1.40
  • HUMNDME
  • malate dehydrogenase
  • Malic enzyme 1
  • malic enzyme 1, NADP(+)-dependent, cytosolic
  • malic enzyme 1, soluble
  • Malic enzyme, cytoplasmic
  • MES
  • NADP-dependent malic enzyme
  • NADP-ME
  • pyruvic-malic carboxylase

Background

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ME1 Antibody (NBP1-32398) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on ME1

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Contact Information

Product PDFs

Calculators

Concentration Calculator

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Bioinformatics

Gene Symbol ME1

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