Lactate Dehydrogenase A/LDHA Antibody [Alexa Fluor® 488]

Flow Cytometry: Lactate Dehydrogenase A/LDHA Antibody [Alexa Fluor® 488] [NBP1-48336AF488] - An intracellular stain was performed on HeLa cells with Lactate Dehydrogenase A/LDHA Antibody NBP1-48336AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

Lactate Dehydrogenase A/LDHA Antibody [Alexa Fluor® 488] Summary

• Immunogen: Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LDHA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lactate Dehydrogenase A/LDHA Antibody [Alexa Fluor® 488]

• Cell proliferation-inducing gene 19 protein
• EC 1.1.1
• EC 1.1.1.27
• GSD11
• Lactate Dehydrogenase A
• lactate dehydrogenase M
• LDH muscle subunit
• LDH1
• LDHA
• LDH-A
• LDHM
• LDH-M
• L-lactate dehydrogenase A chain
• proliferation-inducing gene 19
• Renal carcinoma antigen NY-REN-59

Background

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lactate Dehydrogenase A/LDHA Antibody (NBP1-48336AF488)(1)

Publication using NBP1-48336AF488

• Boyle J, Patronas NJ, Smirniotopoulos J et al CRH stimulation improves 18F-FDG-PET detection of pituitary adenomas in Cushing's disease. Endocrine. 2019-07-01 [PMID: 31062234] (IHC-P, Human)
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.

Bioinformatics

• Gene Symbol: LDHA