iNOS Antibody (4E5) - BSA Free


Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on NIH3T3 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were more
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
Flow Cytometry: iNOS Antibody (4E5) - BSA Free [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119 (blue) and a matched isotype control MAB002 (orange). Cells were more
ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IHC, IHC-Fr, IHC-P
BSA Free
1.0 mg/ml

Order Details

iNOS Antibody (4E5) - BSA Free Summary

iNOS Antibody (4E5) was made to a purified recombinant fragment of human iNOS (C-terminus) expressed in E. coli. [UniProt# P35228]
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA 1:10000
  • Flow (Intracellular)
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32780728)The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29891729).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
NBP2-22119 in the following applications:

Read Publications using
NBP2-22119 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein A purified

Alternate Names for iNOS Antibody (4E5) - BSA Free

  • EC
  • Hepatocyte NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2


Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).


1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NBP2-22119)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

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Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using NBP2-22119 Applications Species
Zheng X, Chen L, Tan J et al. Effect of micro/nano-sheet array structures on the osteo-immunomodulation of macrophages Regenerative biomaterials 2022-10-04 [PMID: 36284748]
Wilkinson HN, Reubinoff B, Shveiky D et al. Epithelial arginase-1 is a key mediator of age-associated delayed healing in vaginal injury Frontiers in endocrinology 2022-08-11 [PMID: 36034415] (IHC-P, Rat) IHC-P Rat
Zhang W, Chen J, Guo W et al. WKYMVm/FPR2 Alleviates Spinal Cord Injury by Attenuating the Inflammatory Response of Microglia Mediators of Inflammation 2022-07-27 [PMID: 35935810] (IHC-P, Rat) IHC-P Rat
Chen Y, Li P, Zhen R et al. Effects of niacin on intestinal epithelial Barrier, intestinal Immunity, and microbial community in weaned piglets challenged by PDCoV International immunopharmacology 2022-08-01 [PMID: 35921778] (WB, Mouse) WB Mouse
Yan B, Tan J, Zhang H et al. Constructing fluorine-doped Zr-MOF films on titanium for antibacteria, anti-inflammation, and osteogenesis Materials Science and Engineering: C 2022-02-01 [PMID: 35581071]
Ganz T, Fainstein N, Elad A et al. Microbial pathogens induce neurodegeneration in Alzheimer's disease mice: protection by microglial regulation Journal of neuroinflammation 2022-01-06 [PMID: 34991645] (IHC-Fr, Mouse) IHC-Fr Mouse
Fan P, Yang Y, Liu T Et Al. Anti-atopic effect of Viola yedoensis ethanol extract against 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin dysfunction Journal of ethnopharmacology 2021-07-28 [PMID: 34332065]
Selvaraj UM, Ujas TA, Kong X et al. Delayed diapedesis of CD8 T cells contributes to long-term pathology after ischemic stroke in male mice Brain, behavior, and immunity 2021-05-05 [PMID: 33964435] (IF/IHC, Mouse) IF/IHC Mouse
Kim G, Hyun C The hyaluronan synthesis inhibitor 7-hydroxy-4-methylcoumarin inhibits LPS-induced inflammatory response in RAW 264.7 macrophage cells Journal of Applied Biological Chemistry 2021-09-30
Shueng Pw, Yu Ly, Chiu Hc Et Al. Early phago-/endosomal escape of platinum drugs via ROS-responsive micelles for dual cancer chemo/immunotherapy Biomaterials 2021-07-07 [PMID: 34252800] (IHC-P) IHC-P
Show All 28 Publications.

Review for iNOS Antibody (NBP2-22119) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22119:
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Images Ratings Applications Species Date Details
Western Blot iNOS NBP2-22119
reviewed by:
Vickneshwaran Vinayagam
WB Human 01/01/2017


ApplicationWestern Blot
Sample TestedPlacental tissue


CommentsNon induced, placental tissue on delivery.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. We are having trouble figuring out the correct dilution for IHC. What are the recommended dilutions for this product’s applications?
    • The dilutions for IHC can vary by catalog number with suggested ranges of anywhere from 1:10 to 1:1000 across our iNOS antibody products. Our recommended dilutions for each application are listed in our products’ data sheets, which can be found on their product webpages. If no dilution guidelines are listed, we recommend running preliminary tests to find the optimal dilution for your experiments.

Secondary Antibodies


Isotype Controls

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria ...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews


Vickneshwaran Vinayagam
Application: WB
Species: Human


Gene Symbol NOS2