iNOS Antibody (4E5)

Images

 
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - Analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow (Intracellular): iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on MCF7 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IHC, IHC-P
Clone
4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

iNOS Antibody (4E5) Summary

Immunogen
Purified recombinant fragment of human iNOS (C-terminal) expressed in E. coli. [UniProt# P35228]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOS2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Flow (Intracellular)
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29891729).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP2-22119 in the following applications:

Publications
Read Publications using
NBP2-22119 in the following applications:

  • 2 publications
  • IHC
    1 publication
  • 2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for iNOS Antibody (4E5)

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22119
Species: Hu, Mu
Applications: WB, ELISA, Flow, Flow-IC, IHC, IHC-P

Publications for iNOS Antibody (NBP2-22119)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Flow, IHC, IHC-P, WB.


Filter By Application
Flow
(2)
IHC
(1)
IHC-P
(2)
WB
(1)
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(2)
Mouse
(2)
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Showing Publications 1 - 7 of 7.
Publications using NBP2-22119 Applications Species
Galvez I, Martin-Cordero L, Hinchado MD Anti-inflammatory effect of beta-2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity Brain Behav. Immun. May 3 2019 [PMID: 31055173] (Flow, Mouse) Flow Mouse
Bastian C, Zaleski J, Stahon K et al. NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels. J. Neurosci. Jul 11 2018 [PMID: 29891729] (IHC, Mouse) IHC Mouse
Pistorius K, Souza PR, De Matteis R et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 [PMID: 29805036] (Flow) Cell Chem Biol.  2018 May 24 [PMID: 29805036] (Flow)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Flow
Omlor AJ, Le DD, Schlicker J et al. Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice. Small. Dec 23 2016 [PMID: 28009478]
Okuyama N, Matsuda S, Yamashita A et al. Human Coronary Thrombus Formation Is Associated With Degree of Plaque Disruption and Expression of Tissue Factor and Hexokinase II. Circ. J. 2015 Sep 03 [PMID: 26346032] (IHC-P, Human) IHC-P Human
Padgett Le, Burg Ar, Lei W, Tse Hm. Loss of nADPH Oxidase-Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes. Diabetes. 2014 Oct 06 [PMID: 25288672] (WB) WB
Wang Y, Wang W, Zhang Y et al. Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug [PMID: 20714866] (IHC-P, Human) IHC-P Human

Review for iNOS Antibody (NBP2-22119) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22119:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot iNOS NBP2-22119
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reviewed by:
Anonymous
WB Human 01/01/2017
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Summary

ApplicationWestern Blot
Sample TestedPlacental tissue
SpeciesHuman
LotA-4

Comments

CommentsNon induced, placental tissue on delivery.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional iNOS Products

Bioinformatics Tool for iNOS Antibody (NBP2-22119)

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Diseases for iNOS Antibody (NBP2-22119)

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Pathways for iNOS Antibody (NBP2-22119)

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PTMs for iNOS Antibody (NBP2-22119)

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Research Areas for iNOS Antibody (NBP2-22119)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

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Anonymous
01/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot