iNOS Antibody (4E5)

Images

 
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Western blot analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - Flow cytometric analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Western blot analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

iNOS Antibody (4E5) Summary

Immunogen
Purified recombinant fragment of human iNOS (C-terminal) expressed in E. coli. [UniProt# P35228]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOS2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This iNOS (4E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP2-22119 in the following applications:

Publications
Read Publications using
NBP2-22119 in the following applications:

  • 2 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for iNOS Antibody (4E5)

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ELISA, Flow, IHC-P

Publications for iNOS Antibody (NBP2-22119)(4)

Review for iNOS Antibody (NBP2-22119) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP2-22119:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot iNOS NBP2-22119
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reviewed by:
Anonymous
WB Human 01/01/2017
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Summary

ApplicationWestern Blot
Sample TestedPlacental tissue
SpeciesHuman
LotA-4

Comments

CommentsNon induced, placental tissue on delivery.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for iNOS Antibody (NBP2-22119)

Discover related pathways, diseases and genes to iNOS Antibody (NBP2-22119). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for iNOS Antibody (NBP2-22119)

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Pathways for iNOS Antibody (NBP2-22119)

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PTMs for iNOS Antibody (NBP2-22119)

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Research Areas for iNOS Antibody (NBP2-22119)

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Blogs on iNOS.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot