iNOS Antibody (4E5)


Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Western blot analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5)and MCF-7 (6) cell lysate.
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - Flow cytometric analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Western blot analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
1.0 mg/ml

Order Details

iNOS Antibody (4E5) Summary

Purified recombinant fragment of human iNOS expressed in E. coli. [UniProt# P35228]
Ammonium sulfate precipitation
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
1.0 mg/ml
Ammonium sulfate precipitation


Application Notes
This iNOS (4E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
131 kDa
Read Publications using
NBP2-22119 in the following applications:

  • 2 publications
  • WB
    1 publication

Reactivity Notes

Human and mouse.

Alternate Names for iNOS Antibody (4E5)

  • EC
  • Hepatocyte NOS
  • HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
  • Inducible NO synthase
  • Inducible NOS
  • INOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS
  • NOS, type II
  • NOS2
  • NOS2A
  • NOS2ANOS type II


Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for iNOS Antibody (NBP2-22119)(3)

Reviews for iNOS Antibody (NBP2-22119) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 1 of 1 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Secondary Antibodies


Isotype Controls

Additional iNOS Antibody (4E5) Products

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Bioinformatics Tool for iNOS Antibody (NBP2-22119)

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Research Areas for iNOS Antibody (NBP2-22119)

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Blogs on iNOS.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol NOS2

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