iNOS Antibody (4E5)

Images

 
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)
Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.
ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, Flow-IC, IHC, IHC-Fr, IHC-P
Clone
4E5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

iNOS Antibody (4E5) Summary

Immunogen
iNOS Antibody (4E5) was made to a purified recombinant fragment of human iNOS (C-terminus) expressed in E. coli. [UniProt# P35228]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NOS2
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Flow (Intracellular)
Application Notes
Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32780728)The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29891729).
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
iNOS Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 3
using
NBP2-22119 in the following applications:

Publications
Read Publications using
NBP2-22119 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.03% Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for iNOS Antibody (4E5)

  • EC 1.14.13.39
  • Hepatocyte NOS
  • HEP-NOS
  • Inducible NO synthase
  • Inducible NOS
  • iNOS
  • nitric oxide synthase 2, inducible
  • nitric oxide synthase 2A (inducible, hepatocytes)
  • nitric oxide synthase, inducible
  • nitric oxide synthase, macrophage
  • NOS type II
  • NOS
  • NOS2
  • NOS2A
  • Peptidyl-cysteine S-nitrosylase NOS2

Background

Nitric oxide (NO) is a colorless, free radical gas that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family including the two constitutive isoforms: brain, bNOS, or neuronal NOS, nNOS (type I) and endothelial cell NOS, eNOS (type III); along with the inducible isoform, iNOS (type II). NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO, requiring the cofactors calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN), heme, and tetrahydrobiopterin (1).

The 131 kDa enzyme, iNOS, is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. While constitutively expressed in kidneys, in other tissues iNOS is induced by bacterial lipopolysaccharides (LPS), growth factors, and cytokines such as IFN-gamma, TNF, IL-1 and IL-2. iNOS is not regulated by the level of intracellular Ca2+ and is constantly active as a dimer when expressed. iNOS activity is elevated in a variety of diseases including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Acting as a critical mediator of inflammation and apoptosis, iNOS inhibitors have been shown to alleviate obesity and stress inducted insulin resistance in mouse models (2,3).

References

1. Forstermann U, and Sessa WC. (2012) Nitric oxide synthases: regulation and function. Eur Heart J. 33(7): 829-837. PMID: 21890489

2. Aktan F. (2004) iNOS-mediated nitric oxide production and its regulation. Life Sci. 75(6):639-53. PMID: 15172174

3. Cinelli MA, Do HT, Miley GP, Silverman RB. (2020) Inducible nitric oxide synthase: Regulation, structure, and inhibition. Med Res Rev. 40(1):158-189. PMID: 31192483

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22119
Species: Hu, Mu
Applications: WB, ELISA, Flow, Flow-IC, IHC, IHC-Fr, IHC-P

Publications for iNOS Antibody (NBP2-22119)(13)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: FLOW, Flow, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
FLOW
(1)
Flow
(5)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
WB
(1)
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Human
(5)
Mouse
(5)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP2-22119 Applications Species
MartIn-Cordero L, Galvez I, Hinchado MD, Ortega E Influence of Obesity and Exercise on beta 2-Adrenergic-Mediated Anti-Inflammatory Effects in Peritoneal Murine Macrophages Biomedicines Nov 30 2020 [PMID: 33266248] (Flow, Human) Flow Human
Chu C, Hu C, Rung S et al. ARG1 could be expressed by human, but not represent for repair bioRxiv Jul 3 2020 (FLOW, Human) FLOW Human
Hu G, Su Y, Kang BH et al. High-throughput phenotypic screen and transcriptional analysis identify new compounds and targets for macrophage reprogramming Nature communications Feb 3 2021 [PMID: 33536439] (Flow, Mouse) Flow Mouse
Tyagi A, Sharma S, Wu K, et al. Nicotine promotes breast cancer metastasis by stimulating N2 neutrophils and generating pre-metastatic niche in lung Nature communications Jan 20 2021 [PMID: 33473115] (Flow, Human) Flow Human
Goldfarb S, Fainstein N, Ben-Hur T Electroconvulsive stimulation attenuates chronic neuroinflammation JCI Insight Sep 3 2020 [PMID: 32780728] (IHC-Fr, Mouse) IHC-Fr Mouse
Nishri Y, Hampton D, Ben-Shushan E et al. Continuous Immune-Modulatory Effects of Human Olig2+ Precursor Cells Attenuating a Chronic-Active Model of Multiple Sclerosis Mol. Neurobiol. 2019 Oct 28 [PMID: 31656989] (Mouse) Mouse
Galvez I, Martin-Cordero L, Hinchado MD Anti-inflammatory effect of beta-2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity Brain Behav. Immun. May 3 2019 [PMID: 31055173] (Flow, Mouse) Flow Mouse
Bastian C, Zaleski J, Stahon K et al. NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels. J. Neurosci. Jul 11 2018 [PMID: 29891729] (IHC, Mouse) IHC Mouse
Pistorius K, Souza PR, De Matteis R et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 [PMID: 29805036] (Flow) Cell Chem Biol. 2018 May 24 [PMID: 29805036] (Flow)

Details:
Citation using the Alexa Fluor 647 form of this antibody.
Flow
Omlor AJ, Le DD, Schlicker J et al. Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice. Small. Dec 23 2016 [PMID: 28009478]
Show All 13 Publications.

Review for iNOS Antibody (NBP2-22119) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-22119:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot iNOS NBP2-22119
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3
reviewed by:
Anonymous
WB Human 01/01/2017
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Summary

ApplicationWestern Blot
Sample TestedPlacental tissue
SpeciesHuman
LotA-4

Comments

CommentsNon induced, placental tissue on delivery.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 2 of 2 FAQs).

  1. We need to evaluate the level of iNOS in Rat sciatic nerve by Western Blot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
    • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.
  2. We are having trouble figuring out the correct dilution for IHC. What are the recommended dilutions for this product’s applications?
    • The dilutions for IHC can vary by catalog number with suggested ranges of anywhere from 1:10 to 1:1000 across our iNOS antibody products. Our recommended dilutions for each application are listed in our products’ data sheets, which can be found on their product webpages. If no dilution guidelines are listed, we recommend running preliminary tests to find the optimal dilution for your experiments.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22119AF350
Alexa Fluor 405 NBP2-22119AF405
Alexa Fluor 488 NBP2-22119AF488
Alexa Fluor 532 NBP2-22119AF532
Alexa Fluor 594 NBP2-22119AF594
Alexa Fluor 647 NBP2-22119AF647
Alexa Fluor 700 NBP2-22119AF700
Alexa Fluor 750 NBP2-22119AF750
Allophycocyanin NBP2-22119APC
Biotin NBP2-22119B
DyLight 350 NBP2-22119UV
DyLight 405 NBP2-22119V
DyLight 488 NBP2-22119G
DyLight 550 NBP2-22119R
DyLight 594 NBP2-22119DL594
DyLight 650 NBP2-22119C
DyLight 680 NBP2-22119FR
DyLight 755 NBP2-22119IR
FITC NBP2-22119F
HRP NBP2-22119H
Janelia Fluor 549 NBP2-22119JF549
Janelia Fluor 646 NBP2-22119JF646
PE NBP2-22119PE
PerCP NBP2-22119PCP

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Bioinformatics Tool for iNOS Antibody (NBP2-22119)

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Blogs on iNOS.

Toll-like receptors in the intestinal epithelial cells
By Jamshed Arslan, Pharm. D., PhD. Toll-like receptors (TLRs) are microbe-sensing proteins that act as first responders to danger signals. TLRs help the intestinal epithelial cells (IECs) recognize commensal bacteria...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Recent Reviews

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5
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4
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1
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Anonymous
01/01/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol NOS2
Entrez
Uniprot