iNOS Antibody (4E5)

Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - Analysis of MCF-7 cells using iNOS mouse mAb (green) and negative control (red).

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.

Western Blot: iNOS Antibody (4E5) [NBP2-22119] - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)

Immunohistochemistry-Paraffin: iNOS Antibody (4E5) [NBP2-22119] - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow (Intracellular): iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on A549 cells with NBP2-22119PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).

Flow Cytometry: iNOS Antibody (4E5) [NBP2-22119] - An intracellular stain was performed on MCF7 cells with iNOS Antibody (4E5) NBP2-22119APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

ELISA: iNOS Antibody (4E5) [NBP2-22119] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, Flow-IC, IHC, IHC-P
• Clone: 4E5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

iNOS Antibody (4E5) Summary

• Immunogen: Purified recombinant fragment of human iNOS (C-terminal) expressed in E. coli. [UniProt# P35228]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NOS2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Flow (Intracellular)
• Application Notes: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Immunohistochemistry reported in scientific literature (PMID: 29891729).
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  iNOS Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for iNOS Antibody (4E5)

• EC 1.14.13.39
• Hepatocyte NOS
• HEP-NOSPeptidyl-cysteine S-nitrosylase NOS2
• Inducible NO synthase
• Inducible NOS
• iNOS
• nitric oxide synthase 2, inducible
• nitric oxide synthase 2A (inducible, hepatocytes)
• nitric oxide synthase, inducible
• nitric oxide synthase, macrophage
• NOS
• NOS, type II
• NOS2
• NOS2A
• NOS2ANOS type II

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for iNOS Antibody (NBP2-22119)(7)

Publications using NBP2-22119

• Galvez I, Martin-Cordero L, Hinchado MD Anti-inflammatory effect of beta-2 adrenergic stimulation on circulating monocytes with a pro-inflammatory state in high-fat diet-induced obesity Brain Behav. Immun. May 3 2019 [PMID: 31055173] (Flow, Mouse)
• Bastian C, Zaleski J, Stahon K et al. NOS3 Inhibition Confers Post-Ischemic Protection to Young and Aging White Matter Integrity by Conserving Mitochondrial Dynamics and Miro-2 Levels. J. Neurosci. Jul 11 2018 [PMID: 29891729] (IHC, Mouse)
• Pistorius K, Souza PR, De Matteis R et al. PDn-3 DPA Pathway Regulates Human Monocyte Differentiation and Macrophage Function Cell Chem Biol May 16 2018 [PMID: 29805036] (Flow) Cell Chem Biol. 2018 May 24 [PMID: 29805036] (Flow)
  
  Details:
  Citation using the Alexa Fluor 647 form of this antibody.
• Omlor AJ, Le DD, Schlicker J et al. Local Effects on Airway Inflammation and Systemic Uptake of 5 nm PEGylated and Citrated Gold Nanoparticles in Asthmatic Mice. Small. Dec 23 2016 [PMID: 28009478]
• Okuyama N, Matsuda S, Yamashita A et al. Human Coronary Thrombus Formation Is Associated With Degree of Plaque Disruption and Expression of Tissue Factor and Hexokinase II. Circ. J. 2015 Sep 03 [PMID: 26346032] (IHC-P, Human)
• Padgett Le, Burg Ar, Lei W, Tse Hm. Loss of nADPH Oxidase-Derived Superoxide Skews Macrophage Phenotypes to Delay Type 1 Diabetes. Diabetes. 2014 Oct 06 [PMID: 25288672] (WB)
• Wang Y, Wang W, Zhang Y et al. Clinical significance of a myeloperoxidase gene polymorphism and inducible nitric oxide synthase expression in cirrhotic patients with hepatopulmonary syndrome. J Huazhong Univ Sci Technolog Med Sci. 2010 Aug [PMID: 20714866] (IHC-P, Human)

Review for iNOS Antibody (NBP2-22119) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 01/01/2017

Summary

• Application: Western Blot
• Sample Tested: Placental tissue
• Species: Human
• Lot: A-4

Comments

• Comments: Non induced, placental tissue on delivery.

FAQs for iNOS Antibody (NBP2-22119). (Showing 1 - 1 of 1 FAQs).

1. We need to evaluate the level of iNOS in Rat sciatic nerve by westernblot. I see that you have few antibodies suitable for that, could you please recommend the best what you have for our purposes?
   • We have 4 iNOS antibodies that are suitable for your experiment. The best choice for you will depend on your specific preferences, in this case the main difference will be the immunogen location. If the immunogen is not important to you, then I would recommend choosing NB300-605 as it gives you the best value and we are able to provide the exact immunogen.

Bioinformatics

• Gene Symbol: NOS2
• Entrez:
  • 4843(Human)
• Uniprot:
  • P35228()