GAPDH Antibody

Images

 
Western Blot: GAPDH Antibody [NB100-56875] - Analysis of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using this antibody. 25 ug/ml.
Western Blot: GAPDH Antibody [NB100-56875] - Analysis using HeLa cells.
Western Blot: GAPDH Antibody [NB100-56875] - Analysis of GAPDH using this antibody at 1:500 in nuclear and cytoplasmic fractions made from Drosophila head extracts. Data courtesy of Dr. Jerry Lin, University of ...read more
Simple Western: GAPDH Antibody [NB100-56875] - Lane view shows a specific band for GAPDH in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Dr, Fe, Ha, Pm, Po, GeSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GAPDH Antibody Summary

Immunogen
Amino acids 73-87 PITIFQERDPSKIKW of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen.
Marker
Cytosolic Marker
Predicted Species
Porcine (93%), Gerbil (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAPDH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:500
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Lysate (NB800-PC1)
Lung Lysate (NB820-59235)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-56875 in the following applications:

Publications
Read Publications using
NB100-56875 in the following applications:

  • IB
    1 publication
  • WB
    52 publications

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in baboon, chimp and macaque, (86%) salamander.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for GAPDH Antibody

  • aging-associated gene 9 protein
  • EC 1.2.1
  • EC 1.2.1.12
  • EC 2.6.99.-
  • G3PD
  • G3PDH
  • GAPDH
  • GAPDPeptidyl-cysteine S-nitrosylase GAPDH
  • glyceraldehyde 3-phosphate dehydrogenase
  • glyceraldehyde-3-phosphate dehydrogenase
  • MGC88685

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67503
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP1-31470
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB100-64792
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr
NBP1-33527
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-501
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Fe, Fi, GP, Ha, Le, Ma, Pm, Rb, Sh, Sq, Ze
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC-Fr, IP
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00026330-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NB500-317
Species: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
7270-IL/CF
Species: Hu
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
H00005478-M01
Species: Hu
Applications: WB, ELISA
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
H00002023-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, IP
NBP2-12933
Species: Hu, Mu, Rt, Po
Applications: WB, PEP-ELISA
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-56875
Species: Hu, Mu, Rt, Ca, Dr, Fe, Ha, Pm, Po, Ge
Applications: WB, Simple Western
Supplier Logo

Publications for GAPDH Antibody (NB100-56875)(68)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Drosophila, Feline, Hamster, Primate.

We have publications tested in 2 applications: IB, WB.


Filter By Application
IB
(1)
WB
(52)
All Applications
Filter By Species
Human
(29)
Mouse
(12)
Rat
(11)
Canine
(1)
Drosophila
(1)
Feline
(1)
Hamster
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 68. Show All 68 Publications.
Publications using NB100-56875 Applications Species
Yokomori H, Ando W, Yoshimura K et al. Increases in endothelial caveolin-1 and cavins correlate with cirrhosis progression Micron 2015 Mar 30 (WB, Human) WB Human
Gyorgy B, Loov C, Zaborowski MP et al. CRISPR/Cas9 Mediated Disruption of the Swedish APP Allele as a Therapeutic Approach for Early-Onset Alzheimer's Disease Mol Ther Nucleic Acids Jun 1 2018 [PMID: 29858078] (WB, Mouse) WB Mouse
Casselbrant A, Softeland JM, Hellstrom M et al. Luminal polyethylene glycol alleviates intestinal preservation injury irrespective of molecular size J. Pharmacol. Exp. Ther. May 8 2018 [PMID: 29739826] (Rat) Rat
Salem K. Copper-zinc superoxide dismutase and glucose metabolism as redox targets for bortezomib resistance in multiple myeloma Thesis. 2014 (WB, Human) WB Human
MatsuYama S, Shirato k, kawase M et al. Middle East respiratory syndrome coronavirus spike protein is not activated directly by cellular furin during viral entry into target cells. J. Virol. Jul 18 2018 [PMID: 30021905] (WB, Human) WB Human
Ochaba J, Morozko EL, O'Rourke JG, Thompson LM. Fractionation for Resolution of Soluble and Insoluble Huntingtin Species. J Vis Exp 2018 Feb 27 [PMID: 29553509]
Gurda BL, Bagel JH, Fisher SJ et al. LC3 Immunostaining in the Inferior Olivary Nuclei of Cats With Niemann-Pick Disease Type C1 Is Associated With Patterned Purkinje Cell Loss J. Neuropathol. Exp. Neurol. 2018 Jan 13 [PMID: 29346563] (WB, Feline) WB Feline
Takakura K, Ito S, Sonoda J et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions Heliyon Jul 9 1905 [PMID: 29264419] (WB, Rat) WB Rat
Yong HEJ, Murthi P, Kalionis B et al. Decidual ACVR2A regulates extravillous trophoblast functions of adhesion, proliferation, migration and invasion in vitro. Pregnancy Hypertens. 2017 Nov 14 [PMID: 29203340] (WB) WB
Mavanji V, Butterick TA, Duffy CM et al. Orexin/hypocretin treatment restores hippocampal-dependent memory in orexin-deficient mice Neurobiol Learn Mem. 2017 Dec 01 [PMID: 29107703] (Mouse) Mouse
Show All 68 Publications.

Reviews for GAPDH Antibody (NB100-56875) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Chinese Hamster, Feline.

Reviews using NB100-56875:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(1)
Chinese Hamster
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GAPDH NB100-56875
Enlarge
reviewed by:
Anonymous
WB Chinese Hamster 10/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedprotein extracts from Lec-2 cells
SpeciesChinese Hamster
Lot04231895B-10

Comments

CommentsI used GAPDH antibody NB100-56875 for Western blot on Lec-2 cells extracts before. It worked really well. Last lot, 04231895B-10, does not recognize hamster GAPDH. I cannot blame Novus since it is polyclonal antibody and they also did not claim that it recognizes hamster GAPDH.
Western Blot GAPDH NB100-56875
Enlarge
reviewed by:
Anonymous
WB Feline 12/09/2016
View

Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesFeline
Western Blot GAPDH NB100-56875
Enlarge
reviewed by:
Anonymous
WB Human 09/01/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
 
reviewed by:
Matt Stratton
WB 05/20/2014
View

Summary

ApplicationWestern Blot

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GAPDH Antibody (NB100-56875). (Showing 1 - 6 of 6 FAQs).

  1. We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
    • Well GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are lookin at. GAPDH antibody is a good choice for low to mid MW targets as GAPDH detects around 35 kDa.
  2. I want to know why GAPDH is used as a loading control antibody in WB techniques.
    • Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
  3. I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
    • This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
  4. I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?
    • Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
  5. What secondary antibody should I use for visualization of the GAPDH antibody?
    • This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
  6. Do you offer a smaller size for the GAPDH antibodies?
    • We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for GAPDH Antibody (NB100-56875)

Discover related pathways, diseases and genes to GAPDH Antibody (NB100-56875). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for GAPDH Antibody (NB100-56875)

Discover more about diseases related to GAPDH Antibody (NB100-56875).
 

Pathways for GAPDH Antibody (NB100-56875)

View related products by pathway.

PTMs for GAPDH Antibody (NB100-56875)

Learn more about PTMs related to GAPDH Antibody (NB100-56875).
 

Research Areas for GAPDH Antibody (NB100-56875)

Find related products by research area.

Blogs on GAPDH. Showing 1-10 of 12 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing west blots. However, this pri...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

Beta Actin and GAPDH: The Importance of Loading Controls
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

New Primers Available for ChIP Procedures
Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
10/13/2017
Application: WB
Species: Chinese Hamster

Anonymous
12/09/2016
Application: WB
Species: Feline

Anonymous
09/01/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol GAPDH
Entrez
Uniprot