GAPDH Antibody

Images

 
Western Blot: GAPDH Antibody [NB100-56875] - analysis of GAPDH in the multiple human tumor cell line lysate INSTA-Blot using this antibody. 25 ug/ml.
Western Blot: GAPDH Antibody [NB100-56875] - Analysis using HeLa cells.
Western Blot: GAPDH Antibody [NB100-56875] - analysis of GAPDH using this antibody at 1:500 in nuclear and cytoplasmic fractions made from Drosophila head extracts. Data courtesy of Dr. Jerry Lin, University of ...read more
Simple Western: GAPDH Antibody [NB100-56875] - Simple Western lane view shows a specific band for GAPDH in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Dr, Fe, PmSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

GAPDH Antibody Summary

Immunogen
Amino acids 73-87 PITIFQERDPSKIKW of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen.
Marker
Cytosolic Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAPDH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Simple Western 1:500
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
Lung Lysate (NB820-59235)
Reviewed Applications
Read 4 Reviews rated 4.8
using
NB100-56875 in the following applications:

Publications
Read Publications using
NB100-56875 in the following applications:

  • IB
    1 publication
  • WB
    46 publications

Reactivity Notes

Cat, Chimpanzee, Macaque. The amino acid sequence used as immunogen is 100% homologous in human, baboon, chimp and macaque, 93% in pig, rat and gerbil, and 86% mouse, cat and salamander.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for GAPDH Antibody

  • aging-associated gene 9 protein
  • EC 1.2.1
  • EC 1.2.1.12
  • EC 2.6.99.-
  • G3PD
  • G3PDH
  • GAPDH
  • GAPDPeptidyl-cysteine S-nitrosylase GAPDH
  • glyceraldehyde 3-phosphate dehydrogenase
  • glyceraldehyde-3-phosphate dehydrogenase
  • MGC88685

Background

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for GAPDH Antibody (NB100-56875)(59)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Drosophila, Hamster, Primate.

We have publications tested in 2 applications: IB, WB.


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IB
(1)
WB
(46)
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Filter By Species
Human
(28)
Mouse
(9)
Rat
(9)
Canine
(1)
Drosophila
(1)
Hamster
(1)
Primate
(1)
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Showing Publications 1 - 10 of 59. Show All 59 Publications.
Publications using NB100-56875 Applications Species
Salcher S, Hermann M, Kiechl-Kohlendorfer U et al. C10ORF10/DEPP-mediated ROS accumulation is a critical modulator of FOXO3-induced autophagy. Mol. Cancer May 25 2017 [PMID: 28545464] (IB, Human) IB Human
Armbruster N, Krieg J, Weibenberger M et al. Rescued Chondrogenesis of Mesenchymal Stem Cells under Interleukin 1 Challenge by Foamyviral Interleukin 1 Receptor Antagonist Gene Transfer. Front Pharmacol May 24 2017 [PMID: 28536528] (WB, Human) WB Human
Liang X, Yang LX, Guo R et al. Atorvastatin attenuates plaque vulnerability by downregulation of EMMPRIN expression via COX-2/PGE2 pathway. Exp Ther Med. Mar 1 2017 [PMID: 28450907] (WB, Mouse) WB Mouse
Li Y, He X, Li J et al. Proliferation and differentiation of direct co-culture of bone marrow mesenchymal stem cells and pigmented cells from the ciliary margin. Mol Med Rep. Apr 19 2017 [PMID: 28440470] (WB, Rat) WB Rat
Shao Z, Lee X, Huang G et al. LINGO-1 Regulates Oligodendrocyte Differentiation through the Cytoplasmic Gelsolin Signaling Pathway. J. Neurosci. 2017 Feb 13 [PMID: 28193690] (WB, Human) WB Human
Vonk JJ, Yeshaw WM, Pinto F et al. Drosophila Vps13 Is Required for Protein Homeostasis in the Brain PLoS ONE Jan 20 2017 [PMID: 28107480] (WB, Drosophila) WB Drosophila
Zhong L, Simoneau B, Huot J, Simard MJ. p38 and JNK pathways control E-selectin-dependent extravasation of colon cancer cells by modulating miR-31 transcription. Oncotarget. Jan 3 2017 [PMID: 27926494] (WB, Human) WB Human
Gunatillake T, Yong HE, Dunk C et al. Homeobox gene TGIF-1 is increased in placental endothelial cells of human fetal growth restriction Reproduction Aug 18 2016 [PMID: 27539603] (WB, Human) WB Human
Vargas DA, Sun M, Sadykov K et al. The Integrated Role of Wnt/beta-Catenin, N-Glycosylation, and E-Cadherin-Mediated Adhesion in Network Dynamics PLoS Comput. Biol. 2016 Jul 01 [PMID: 27427963] (WB) WB
Jagessar SA, Holtman IR, Hofman S et al. Lymphocryptovirus Infection of Nonhuman Primate B Cells Converts Destructive into Productive Processing of the Pathogenic CD8 T Cell Epitope in Myelin Oligodendrocyte Glycoprotein J. Immunol. 2016 Jul 13 [PMID: 27412414] (WB) WB
Show All 59 Publications.

Reviews for GAPDH Antibody (NB100-56875) (4) 4.84

Average Rating: 4.8
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Chinese Hamster, Feline.
We have 4 reviews tested in 1 application: WB.

Reviews using NB100-56875:
Filter by Applications
WB
(4)
All Applications
Filter by Species
Human
(1)
Chinese Hamster
(1)
Feline
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot GAPDH NB100-56875
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reviewed by:
Anonymous
WB Chinese Hamster 10/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedprotein extracts from Lec-2 cells
SpeciesChinese Hamster
Lot04231895B-10

Comments

CommentsI used GAPDH antibody NB100-56875 for Western blot on Lec-2 cells extracts before. It worked really well. Last lot, 04231895B-10, does not recognize hamster GAPDH. I cannot blame Novus since it is polyclonal antibody and they also did not claim that it recognizes hamster GAPDH.
Western Blot GAPDH NB100-56875
Enlarge
reviewed by:
Anonymous
WB Feline 12/09/2016
View

Summary

ApplicationWestern Blot
Sample TestedHeart lysates
SpeciesFeline
Western Blot GAPDH NB100-56875
Enlarge
reviewed by:
Anonymous
WB Human 09/01/2015
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF
 
reviewed by:
Matt Stratton
WB 05/20/2014
View

Summary

ApplicationWestern Blot

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GAPDH Antibody (NB100-56875). (Showing 1 - 6 of 6 FAQs).

  1. We need a loading control antibody for WB working with HeLa cell line, will this GAPDH antibody work for that?
    • Well GAPDH is expressed in HeLa cell lines but whether or not it is the best option for a loading control antibody depends on the size of the protein you are lookin at. GAPDH antibody is a good choice for low to mid MW targets as GAPDH detects around 35 kDa.
  2. I want to know why GAPDH is used as a loading control antibody in WB techniques.
    • Our GAPDH antibody makes a good loading control because GAPDH is  a housekeeping gene essential to metabolism that is globally expressed in most tissue types and cell lines.
  3. I need a suitable loading control antibody that I can use in western blot of rat nerve extracts, which do you recommend?
    • This GAPDH antibody (NB300-221) is a good choice for a loading control antibody and has been cited in over 180 publications and used on rat lysates with good results.
  4. I'm looking for a loading control between 24kDa - 65kDa for use in simple western, we tried an actin antibody  we already had but it didn't work. What can you recommend?
    • Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements.
  5. What secondary antibody should I use for visualization of the GAPDH antibody?
    • This GAPDH antibody is raised in mouse so you would want to use an anti-mouse secondary with the dye of your choice.
  6. Do you offer a smaller size for the GAPDH antibodies?
    • We offer sample sizes for many of our GAPDH loading control antibodies including NB300-221.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional GAPDH Products

Bioinformatics Tool for GAPDH Antibody (NB100-56875)

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Diseases for GAPDH Antibody (NB100-56875)

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Blogs on GAPDH. Showing 1-10 of 12 blog posts - Show all blog posts.

The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...  Read full blog post.

Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...  Read full blog post.

GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing west blots. However, this pri...  Read full blog post.

A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...  Read full blog post.

GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...  Read full blog post.

GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...  Read full blog post.

Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...  Read full blog post.

Beta Actin and GAPDH: The Importance of Loading Controls
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti...  Read full blog post.

The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...  Read full blog post.

New Primers Available for ChIP Procedures
Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

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Recent Reviews

4.8
5
3
4
1
3
0
2
0
1
0

Anonymous
10/13/2017
Application: WB
Species: Chinese Hamster

Anonymous
12/09/2016
Application: WB
Species: Feline

Anonymous
09/01/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol GAPDH
Entrez
Uniprot