Enolase 1 Antibody (8G8)

Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.Lane 1: ENO1 transfected lysate(47 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 1 ug/ml.

Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - Analysis of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ENO1 MaxPab rabbit polyclonal antibody.

Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8 Analysis of ENO1 expression in MCF-7.

Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - ENO1 monoclonal antibody (M01), clone 8G8. Analysis of ENO1 expression in human colon.

ELISA: Enolase 1 Antibody (8G8) [H00002023-M01] - Detection limit for recombinant GST tagged ENO1 is approximately 0.3ng/ml as a capture antibody.

Western Blot: Enolase 1 Antibody (8G8) [H00002023-M01] - LHPP suppresses aerobic glycolysis. Protein expression levels of Enolase 1 in LHPP-overexpressing or LHPP-knockdown MGC-803 cells and control cells on western blot. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35568711/) licensed under a CC-BY license.

• Reactivity: Hu, Fe
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC, IP
• Clone: 8G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Enolase 1 Antibody (8G8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK
• Specificity: ENO1 - enolase 1, (alpha)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein, transfected lysate and cell lysate for WB. It has been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Enolase 1 Antibody (8G8)

• 2-phospho-D-glycerate hydro-lyase
• Alpha enolase
• C-myc promoter-binding protein
• c-myc promoter-binding protein-1
• EC 4.2.1
• EC 4.2.1.11
• ENO1L1
• Enolase 1
• enolase 1, (alpha)
• MBP-1
• MBPB1
• MPB-1
• MPB1c-myc promoter-binding protein 1
• MYC promoter-binding protein 1
• NNE
• Non-neural enolase
• Phosphopyruvate hydratase
• Plasminogen-binding protein
• PPHalpha enolase like 1
• tau-crystallin

Background

This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Enolase 1 Antibody (H00002023-M01)(10)

Publications using H00002023-M01

• Huppertz I, Perez-Perri JI, Mantas P et al. Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation Molecular cell 2022-06-10 [PMID: 35709751] (WB)
• Lin J, Lian N, Gao Y Et Al. m6A Methylation Mediates LHPP Acetylation as a Tumour Aerobic Glycolysis Suppressor to Improve the Prognosis of Gastric Cancer Cell Death Dis 2022-05-14 [PMID: 35568711]
• McLeland S, Quimby J, Lappin M et al. Alpha-enolase staining patterns in the renal tissues of cats with and without chronic kidney disease. Vet Immunol Immunopathol. 2019-05-01 [PMID: 31213248]
• Zakrzewicz D, Didiasova M, Kruger M et al. Protein arginine methyltransferase 5 mediates enolase-1 cell surface trafficking in human lung adenocarcinoma cells. Biochim Biophys Acta 2018-03-02 [PMID: 29501774]
• Tohgasaki T, Ozawa N, Yoshino T et al. Enolase-1 expression in the stratum corneum is elevated with parakeratosis of atopic dermatitis and disrupts the cellular tight junction barrier in keratinocytes. Int J Cosmet Sci 2018-02-11 [PMID: 29430682]
• Zakrzewicz D, Didiasova M, Zakrzewicz A et al. The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization. Biochem J. 2014-06-01 [PMID: 24628430]
• Chu PY, Hsu NC, Liao AT et al. Overexpression of alpha-enolase correlates with poor survival in canine mammary carcinoma. BMC Vet Res. 2011-10-21 [PMID: 22014164]
• Yu Y, Shen H, Yu H et al. Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis. Mol Biosyst 2011-04-06 [PMID: 21468425]
• Ho JA, Chang HC, Shih NY et al. Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor. Anal Chem. 2010-06-17 [PMID: 20557064]
• Zhao J, Chang AC, Li C et al. Comparative proteomic analysis of Barrett's metaplasia and esophageal adenocarcinomas using 2-D liquid mass mapping. Mol Cell Proteomics 2006-07-08 [PMID: 16829691]

Bioinformatics

• Gene Symbol: ENO1
• Entrez:
  • 2023(Human)
• OMIM:
  • 172430(Human)
• Uniprot:
  • AAH22545(Human)