FXR/NR1H4 Antibody

Images

 
Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.
Immunocytochemistry/ Immunofluorescence: FXR Antibody [NB400-153] - FXR antibody was tested in HEK293 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
5.7 mg/ml

FXR/NR1H4 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus of the human FXR protein. [UniProt# Q96RI1]
Localization
Cytoplasmic, nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR1H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
This FXR antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis, where a band is seen at ~66 kDa. In ICC/IF nuclear and cytoplasmic staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC9)
Control Peptide
FXR/NR1H4 Peptide (NB400-153PEP)
Publications
Read Publication using NB400-153.

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
5.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FXR/NR1H4 Antibody

  • BAR
  • bile acid receptor
  • farnesoid X receptor
  • Farnesoid X-activated receptor
  • Farnesol receptor HRR-1
  • FXR
  • FXRBAR
  • HRR-1
  • HRR1MGC163445
  • NR1H4
  • Nuclear receptor subfamily 1 group H member 4
  • nuclear receptor subfamily 1, group H, member 4
  • Retinoid X receptor-interacting protein 14
  • RIP14
  • RIP14RXR-interacting protein 14

Background

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR is mainly expressed in liver, intestine, kidney, and adrenal glands. FXR heterodimerizes with retinoid X receptor (RXR alpha/NR2B1), associates with FXREs (FXR response elements) that are usually but not exclusively inverted repeat-1 (IR-1), and regulates the transcription of its target genes. FXR interacts with CARM1, SMARD1 and after activation by agonist binding, it interacts with a coactivator, NCOA1 or NCOA2. FXR has been shown to repress transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase gene) through induction of NR0B2 or FGF19 expression, via two distinct mechanisms. FXR activates the intestinal bile acid-binding protein (IBABP) and transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Altogether, FXR is critical for maintaining a homeostasis in bile acid, cholesterol, lipoprotein, and triglyceride metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FXR/NR1H4 Antibody (NB400-153)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

View specific protocols for FXR/NR1H4 Antibody (NB400-153): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FXR/NR1H4 Antibody (NB400-153) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-153AF405
Alexa Fluor 488 Labeled NB400-153AF488
Alexa Fluor 647 Labeled NB400-153AF647
Alexa Fluor 700 Labeled NB400-153AF700
Biotin Labeled NB400-153B
DyLight 405 Labeled NB400-153V
DyLight 488 Labeled NB400-153G
DyLight 550 Labeled NB400-153R
DyLight 650 Labeled NB400-153C
FITC Labeled NB400-153F
HRP Labeled NB400-153H

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Bioinformatics

Gene Symbol NR1H4
Entrez