FXR/NR1H4 Antibody

Images

 
Western Blot: FXR/NR1H4 Antibody [NB400-153] - Total protein from human A549, U2OS cell and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. ...read more
Immunocytochemistry/ Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - A549 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more
Western Blot: FXR Antibody [NB400-153] - Detection of FXR in HeLa nuclear extract using NB400-153. ECL 30 minute detection.
Immunocytochemistry/ Immunofluorescence: FXR/NR1H4 Antibody [NB400-153] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

FXR/NR1H4 Antibody Summary

Immunogen
A synthetic peptide made to the C-terminus of the human FXR protein. [UniProt# Q96RI1]
Localization
Cytoplasmic, nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR1H4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 4 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Nuclear Cell Lysate
Control Peptide
FXR/NR1H4 Peptide (NB400-153PEP)
Reviewed Applications
Read 3 Reviews rated 4.7
using
NB400-153 in the following applications:

Publications
Read Publications using
NB400-153 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29491146).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine, 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FXR/NR1H4 Antibody

  • BAR
  • bile acid receptor
  • farnesoid X receptor
  • Farnesoid X-activated receptor
  • Farnesol receptor HRR-1
  • FXR
  • FXRBAR
  • HRR-1
  • HRR1MGC163445
  • NR1H4
  • Nuclear receptor subfamily 1 group H member 4
  • nuclear receptor subfamily 1, group H, member 4
  • Retinoid X receptor-interacting protein 14
  • RIP14
  • RIP14RXR-interacting protein 14

Background

FXR (farnesoid X receptor; NR1H4) belongs to nuclear receptor superfamily of ligand-activated transcription factors (NR1 subfamily) and acts as a receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. FXR is mainly expressed in liver, intestine, kidney, and adrenal glands. FXR heterodimerizes with retinoid X receptor (RXR alpha/NR2B1), associates with FXREs (FXR response elements) that are usually but not exclusively inverted repeat-1 (IR-1), and regulates the transcription of its target genes. FXR interacts with CARM1, SMARD1 and after activation by agonist binding, it interacts with a coactivator, NCOA1 or NCOA2. FXR has been shown to repress transcription of CYP7A1 (cholesterol 7-alpha-hydroxylase gene) through induction of NR0B2 or FGF19 expression, via two distinct mechanisms. FXR activates the intestinal bile acid-binding protein (IBABP) and transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Altogether, FXR is critical for maintaining a homeostasis in bile acid, cholesterol, lipoprotein, and triglyceride metabolism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-153
Species: Hu, Mu
Applications: WB, ICC/IF

Publications for FXR/NR1H4 Antibody (NB400-153)(3)

Reviews for FXR/NR1H4 Antibody (NB400-153) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NB400-153:
Filter by Applications
ICC
(1)
WB
(2)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry FXR/NR1H4 NB400-153
Enlarge
reviewed by:
Anonymous
ICC Human 04/04/2020
View

Summary

ApplicationImmunocytochemistry
Sample Tested hela cell
SpeciesHuman
LotA
Western Blot FXR/NR1H4 NB400-153
Enlarge
reviewed by:
Anonymous
WB Human 12/27/2019
View

Summary

ApplicationWestern Blot
Sample Tested Hela whole cell lysate
SpeciesHuman
LotA
Western Blot FXR/NR1H4 NB400-153
Enlarge
reviewed by:
Anonymous
WB Human 02/19/2019
View

Summary

ApplicationWestern Blot
Sample TestedHepG2,Human liver cancer cell line (HuH7)
SpeciesHuman
LotA

Product General Protocols

View specific protocols for FXR/NR1H4 Antibody (NB400-153): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FXR/NR1H4 Antibody (NB400-153) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-153AF350
Alexa Fluor 405 NB400-153AF405
Alexa Fluor 488 NB400-153AF488
Alexa Fluor 532 NB400-153AF532
Alexa Fluor 594 NB400-153AF594
Alexa Fluor 647 NB400-153AF647
Alexa Fluor 700 NB400-153AF700
Alexa Fluor 750 NB400-153AF750
Biotin NB400-153B
DyLight 350 NB400-153UV
DyLight 405 NB400-153V
DyLight 488 NB400-153G
DyLight 550 NB400-153R
DyLight 594 NB400-153DL594
DyLight 650 NB400-153C
DyLight 680 NB400-153FR
DyLight 755 NB400-153IR
FITC NB400-153F
HRP NB400-153H
Janelia Fluor 549 NB400-153JF549
Janelia Fluor 646 NB400-153JF646

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Bioinformatics Tool for FXR/NR1H4 Antibody (NB400-153)

Discover related pathways, diseases and genes to FXR/NR1H4 Antibody (NB400-153). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for FXR/NR1H4 Antibody (NB400-153)

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PTMs for FXR/NR1H4 Antibody (NB400-153)

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Research Areas for FXR/NR1H4 Antibody (NB400-153)

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Blogs on FXR/NR1H4

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Anonymous
04/04/2020
Application: ICC
Species: Human

Anonymous
12/27/2019
Application: WB
Species: Human

Anonymous
02/19/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol NR1H4